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. Author manuscript; available in PMC: 2019 Aug 11.
Published in final edited form as: Oncogene. 2019 Feb 11;38(24):4700–4714. doi: 10.1038/s41388-019-0745-2

Figure 2. KPNB1 knockdown inhibits PCa growth.

Figure 2.

A and B) Crystal violet staining showing the growth of indicated cell lines with KPNB1 knockdown. Both cell lines were transfected with KPNB1 siRNA (50 nM) or control siRNA for 24 hours, 48 hours and 72 hours respectively. C) MTT assay showing the relative cell viabilities of indicated cell lines that were transfected with KPNB1 siRNA (50 nM) or control siRNA for 48 hours. D and E) Flow cytometry showing the cell cycle stage distribution of indicated cell lines that were transfected with KPNB1 siRNA (50 nM) or control siRNA for 48 hours. F) Immunoblotting results showing protein level of KPNB1, Cyclin D1, Cyclin B1, CDK1, RCC1 and pRCC1 of indicated cell lines that were transfected with KPNB1 siRNA (50 nM) or control siRNA for 48 hours. GAPDH was used as internal control. G) Immunoblotting results showing physical interaction between KPNB1 and c-Myc proteins by immunoprecipitation assay in both of C42B and PC3 cell lines. H) Immunoblotting results showing KPNB1 and c-Myc from nuclear extracts of C42B and PC3 that were transfected with KPNB1 siRNA (50 nM) or control siRNA for 48 hours. *p <0.05, **p<0.01, *** p<0.001, ****p<0.0001.