Abstract
Purpose: To investigate whether Niban protein plays a role in renal interstitial fibrosis by regulating renal tubular epithelial cell apoptosis and explore the underlying mechanism.
Methods: Unilateral ureteral obstruction (UUO) model was performed in C57B/6J mice, and divided into sham operation group and groups of days 3, days 7, and days 14. Niban expression was detected by immunohistochemistry and Western blot. TUNEL assays were used to detected apoptosis. Niban siRNA and overexpression Niban plasmid were transfected in HK-2 cells respectively to explore apoptosis related mechanisms of Niban during angiotensin II (AngII) – and endoplasmic reticulum (ER) stress-induced injury.
Results: With the development of obstruction, Niban’s expression decreased gradually while apoptosis increased. Silencing of Niban not only increased the AngII- and ER stress-induced apoptosis, but also promoted the expression of caspase 8, caspase 9, Bip, and Chop. Overexpression of Niban reduced AngII-induced apoptosis and the expression of caspase 8 and caspase 9.
Conclusions: Niban protein is involved in apoptosis regulation in HK-2 cells, and most likely via caspase-dependent pathway.
Keywords: Apoptosis, renal interstitial fibrosis, Niban, chronic kidney disease, unilateral ureteral obstruction
Introduction
Chronic kidney disease (CKD) is increasingly considered a global public health problem [1–3]. Renal interstitial fibrosis (RIF) is the common pathway and the main pathological basis for the progression of various CKDs to end-stage renal disease (ESRD) [4]. The cellular mechanisms leading to renal fibrosis are complex, including inflammation, oxidative stress, apoptosis, and senescence in the form of proximal tubule cell death [5]. A number of studies have shown that cell stress or damage caused by fibrotic stimuli mainly occurs in the renal tubular epithelial compartment, especially the proximal tubule [6,7]. Evidence from the mouse acute kidney injury (AKI) model indicates that apoptosis is more closely related to necrosis than the level of renal insufficiency [8]. Apoptosis can be activated by binding to various death receptors such as Fas/CD95 ligand, tumor necrosis factor (TNF)-α and TNF (ligand) superfamily, members 10 (TNFSF10). Renal tubular cells express these cell surface death receptors, activate caspases in the kidney and initiate apoptosis [9]. Studies have shown that knocking out Bax and Bak genes (pro-apoptotic factors) can block renal tubular cell apoptosis without affecting tubular necrosis, thereby achieving the effect of protecting renal tubular cells [10]. Apoptosis is considered one of the most important mechanisms that promote fibrosis, constituting a research hotspot.
Niban, also termed FAM129A or C1orf24, was originally identified from a rat with renal carcinoma displaying TSC2 gene mutation. It is elevated in animal models and patients with various tumors, playing an important role in regulating gene transcription, protein synthesis, cell proliferation, and apoptosis in tumor cells [11–13]. Our previous study first found that Niban not only regulates apoptosis in tumor cells, but also participates in in the apoptosis of renal tubular epithelial cells, and may be involved in RIF, but the specific mechanism of action remains unclear [14]. Therefore, the present study investigates the mechanism by which Niban alters renal interstitial fibers by affecting apoptosis in renal tubular epithelial cells.
Methods
Animal model establishment
A total of 24 SPF grade C57BL/6J male mice (18–25 g) were purchased from Hunan Slack Jingda Experimental Animal Limited (Changsha, China). The entire animal study was performed at the Animal Experimental Department of Central South University. Mice were divided into four groups according to the principle of complete randomization, including sham operation animals, and UUO model mice treated at 3, 7, and 14 days. Six mice were caged to provide feed and water without restriction. UUO surgery was performed one week after adaptive feeding, and fasting was started one day before surgery. According to animal weight, 0.3–0.4 mL/100 g of 10% chloral hydrate was intraperitoneally injected. The operation was completed in aseptic conditions. Mice were sacrificed at 3, 7, and 14 days. Half of the obstructed kidney tissue was soaked with 4% formalin, for H&E staining, Masson staining, and immunohistochemistry; the other half of the kidney tissue was stored in liquid nitrogen for subsequent Western blot experiments.
Cell culture experiments
The human proximal renal tubular epithelial HK-2 cells line was purchased from American Type Culture Collections (ATCC, Rockville, MD, USA). HK-2 cells were grown in DMEM/F12 (Gibco, Carlsbad, CA, lot 8117270) supplemented with 10% fetal bovine serum (FBS, BI), 100 U/mL penicillin (Gibco, Carlsbad, CA, BRL), and 100 U/mL streptomycin (Gibco, Carlsbad, CA, BRL), in an incubator containing 5% CO2 at 37 °C. HK-2 cells were plated in six-well plates the day before staining and treated with OPTI (Gibco, Carlsbad, CA, 1869014). After overnight incubation (50–70% confluency), the cells were transfected with the Niban plasmid and siRNA, respectively, using liposome lip2000 (Invitrogen, Carlsbad, CA, lot1888645). After 5–6 h of incubation as above, serum-free complete culture medium was used to replace the transfection medium. After one day of synchronized culture, the cells were stimulated with AngII (10–6 mol/L) for 48 h, and proteins were collected for Western blot. Using TM (3 μg/mL) to stimulated HK-2 cells for 24 h, proteins were collected for Western blot.
Antibodies and reagents
The Niban plasmid was purchased from Shandong Weizhen Biotechnology Ltd. (Zibo, China) and Vigene Biosciences (Rockville, MD, USA); siRNA was from Guangzhou Ribobio Biological Ltd. (Guangzhou, China). Antibodies against collagens I and III, and α-SMA were purchased from Abcam (Cambridge, UK). Anti-Niban (21333-1-AP); cleaved-caspase 12 (55238-1-AP); b-actin (20536-1-AP); E-cadherin (20874-1-AP); Bip (115871-1-AP); Chop (15204-1-AP); P53 (10442-1-AP) antibodies were obtained from Proteintech (Rosemont, IL). Antibodies targeting cleaved-caspase 3 (CST 9664) were purchased from Cell Signaling Technology (Danvers, MA). Antibodies targeting cleaved-caspase 8 (ab108333) and cleaved-caspase 9 (ab202068) were purchased from Abcam (Cambridge, UK). Anti-AngII antibody was purchased from Santa Cruz (Santa Cruz, CA). Tunicamycin (TM) was purchased from Meilunbio (Nanning, China, MB 5419). Lip2000 was purchased from Invitrogen (Carlsbad, CA).
Immunohistochemistry
Animal tissue samples were embedded in paraffin, and dewaxed (59 °C) and hydrated overnight. Sodium citrate buffer solution was used at boiling for antigen retrieval. After blocking with 1% BSA, primary antibodies (Niban, 1:100; E-cadherin, 1:200; COL I, 1:500; COL III, 1:500) were applied overnight, respectively. Then, secondary antibodies were applied for 1 h. After DAB development, the samples were counterstained with hematoxylin, resin sealed and observed under a microscope.
TUNEL assay
Apoptosis of renal tissue of UUO mouse was measured with terminal deoxynucleotidyl transferase-mediated (dUTP) nick end-labeling (TUNEL) staining (Cell Death Detection Kit, Roche, Penzberg, Germany). Paraffin-embedded tissue was treated in protocols from TUNEL Kit. Slides were developed with DAB and counterstained with 10% hematoxylin in the end. Apoptosis rate analysis was performed by Image-pro plus 6.0 (Media Cybernetics, Inc., Rockville, MD, USA).
Western blot
As described above, protein samples were collected from animal experiments and cell assays for immunoblot. Anti-Niban, E-cadherin, COL I, α-SMA, cleaved-caspase 3, cleaved-caspase 8, cleaved-caspase 9, cleaved-caspase 12, and β-actin primary antibodies were all diluted at 1:1000 and incubated with samples at 4 °C overnight. Development was performed on an Odyssey two-color infrared laser imaging system. The Image J software was used for protein quantification; results are expressed as percentage change in average band density compared to the corresponding control value.
Transfection
Cells were seeded in six-well plates (6 × 105 cells/well) and cultured with OPTI overnight to 50–70% confluency. Then, transfection was performed with siRNA (50 ng/well) and Niban plasmid (4 µg/well) in HK-2 cells using lipo2000 (5 µL/well). After culture with normal serum without antibiotics, the medium was replaced by complete medium after 5–6 h. Then, synchronized cultures were performed for 24 h, and cells were stimulated with AngII (10–6 mol/L) for 48 h. Finally, cell total protein was extracted for subsequent experiments; overexpression and silencing efficiencies were detected by Western blot.
Statistical analysis
Student's t test was used for group pair comparisons, while multiple groups were compared by analysis of variance (ANOVA). Each experiment was repeated at least three times with similar results. Statistical analysis was performed by the SPSS 18.0 software (SPSS, Chicago, IL, USA). p < .05 was considered statistically significant.
Results
Renal interstitial fibrosis in UUO mouse models
Morphological changes in the obstructed kidney could be observed by the naked eye after 14 days of UUO modeling. Compared with the control group, the UUO group showed significantly enlarged and swollen kidney with brighter surface, thinner cortex, increased amounts of urine, and fluid obstruction (Figure 1(b)). H&E staining, Masson staining, and immunohistochemistry were used to assess interstitial fibrosis in UUO mice. H&E staining was performed respectively in SHM group and UUO group at days 3, 7, and 14, and qualitative damage to the kidney is shown in Figure 1(a). With the extension of UUO modeling time, renal tubular cells gradually shrank, and luminal vacuolization, myofibroblast proliferation, and inflammatory cell accumulation were observed, these pathological changes were most significant at 14 days. At 14 days after UUO, the score of tubulointerstitial injury in mice was significantly higher than that in SHM mice (Figure 1(c)).
Collagen and E-cadherin are effective indicators of fibrosis. Masson staining showed that, compared with SHM control group, collagen expression levels increased with the development of UUO obstruction, and collagen deposition was greater, especially at 14 days, which was consistent with the immunohistochemical results of COL I, COL III, and E-cadherin (Figure 1(a)). In addition, Western blot detecting col I (Figure 1(d)) and E-cadherin (Figure 1(e)) further confirmed the fibrosis induction effect, especially at 14 days in UUO mice.
Expression levels of Niban in UUO mice
Immunohistochemistry and Western blot were used to detect the expression levels of Niban in UUO mice. Immunohistochemical data (Figure 2(a)) showed that Niban in SHM group was significantly expressed, in both glomeruli and renal tubules, but mainly found in renal tubular epithelial cells. With the development of UUO obstruction, Niban expression was gradually decreased. This was consistent with Western blot findings (Figure 2(b)), indicating that Niban levels decreased with the development of renal fibrosis.
Apoptosis in the UUO model
As shown in Figure 3(a), TUNEL assay showed that apoptosis increased gradually with the extension of UUO obstruction time and peaked at seven days. The expression of cleaved-caspase 3 was detected by Western blot in UUO mice, as shown in Figure 3(b,c). Compared with SHM group, the level of cleaved-caspase 3 was significantly increased in UUO group on day 3 and day 7 (both p < .05). There was no statistical significance on day 14 (p > .05).
Endoplasmic reticulum (ER) stress occurs during the course of UUO-induced renal fibrosis
The expression of Bip, Chop, and P53 in UUO mice was detected by Western blot. As shown in Figure 4, the levels of Bip, Chop, and P53 in UUO group on day 7 were significantly higher than those in SHM group (both p < .01); there was no statistical significance on days 3 and 14 (p > .05).
Expression levels of Niban in HK-2 cells stimulated by AngII
AngII is a common inducer of apoptosis. First, we stimulated HK-2 cells with different concentrations of AngII (10−5, 10−6, and 10−7 µmol/L) for 48 h. Then, the expression levels of Niban, E-cadherin, and α-SMA were detected by Western blot (Figure 5(c)). When cells were stimulated with AngII at 10−6 µmol/L, the expression levels of Niban and E-cadherin were decreased, while α-SMA expression was increased. Therefore, we selected 10−6 µmol/L for subsequent experiments. In order to evaluate the expression of Niban in HK-2 cells and further investigate its role in fibrosis, we silenced Niban by siRNA technology and transfected the overexpressed Niban plasmid, respectively. Then, the cells were stimulated with AngII (10−6 µmol/L) to induce apoptosis. After 48 h, the total protein was obtained for Western blot (Niban, E-cadherin, and α-SMA).
Silencing the expression of Niban increased apoptosis and fibrosis in HK-2 cells
As shown in Figure 5(a,b), AngII (10−6 µmol/L) stimulated normal HK-2 cells for 48 h, and observed cell morphology under the microscope. HK-2 cells in control group were oval and grew like a paving stone in a six-well plate. After stimulation with AngII, the cells gradually formed a fusiform shape, and overtly long spindles were observed under the microscope. In addition, the cell density was not as high as that of the control group. The Niban siRNA group also had morphological changes, with cells becoming long with stripped spindles, especially, the number of cells in the Niban siRNA + AngII group was significantly lower than that in the control group. The morphology of cells transfected with the Niban plasmid showed no overt changes, even after AngII stimulation, cell morphology was basically elliptical, and cell number was slightly lower than that of the control group (p > .05). This may be due to the toxic side effects of the transfection reagent.
After stimulation of HK-2 cells with AngII (10−6 µmol/L) for 48 h, the expression levels of Niban and E-cadherin were significantly decreased, while α-SMA expression was markedly increased (all p < .05). After Niban silencing, E-cadherin expression was significantly lower than the control group (p < .01), while α-SMA levels were significantly increased (p < .01). There was no significant difference between the Niban siRNA and Niban siRNA + AngII groups. These results are summarized in Figure 6.
In order to evaluate the role of Niban in regulating the apoptosis of HK-2 cells, we further examined the marker proteins cleaved-caspase 8, cleaved-caspase 9, and cleaved-caspase 12 in three classical apoptotic pathways induced by AngII, including the Fas/FasL pathway, the mitochondrial stress pathway, and ER stress pathway, respectively [15–17]. As shown in Figure 6, the expression levels of cleaved-caspase 8 and cleaved-caspase 9 were increased after stimulation with AngII (p < .05). Especially in the Niban siRNA and Niban siRNA + AngII groups, the expression levels of cleaved-caspase 8 and cleaved-caspase 9 were significantly increased (p < .01). Meanwhile, there was no significant change in the expression of cleaved-caspase 12 compared with the control group.
Overexpression of Niban reduced apoptosis and relieved renal fibrosis
Compared with the control group, the expression levels of Niban and E-cadherin were decreased after AngII stimulation, while the expression levels of α-SMA were increased (all p < .05) (Figure 7). After Niban was transfected into HK-2 cells, its expression was significantly increased compared with that of the control group (p < .01). When AngII was used to stimulate HK-2 cells, the levels of Niban, E-cadherin, α-SMA, cleaved-caspase 8, and cleaved-caspase 9 did not change significantly compared with the Niban pENTER group (p > .05); cleaved-caspase 12 also showed no changes.
Silencing the expression of Niban increased endoplasmic reticulum stress
Using TM as an inducer of ER stress; as shown in Figure 8, after stimulation with TM, the expression of Niban in HK-2 cells decreased, while the expression of ER stress marker proteins Bip and Chop was significantly increased (p < .01). After inhibiting Niban expression, Bip and Chop of HK-2 cells increased before TM stimulation, but there was no statistical significance (p > .05). After TM stimulation, Bip and Chop of HK-2 cells were significantly increased (p < .001). When the Niban siRNA + TM group was compared with the control + TM group, Niban decreased (p < .05), while the Bip and Chop increased (both p < .05).
Discussion
Ureteral obstruction leads to kidney damage and eventually causes irreversible RIF, with extracellular matrix deposition, inflammatory cell infiltration, tubular cell damage, and apoptosis. Apoptosis of renal tubular epithelial cell apoptosis occurs in the early stage of ureteral obstruction. A series of signals, including inflammatory factors and chemokines, are released after cell apoptosis, eventually leading to inflammatory reactions and fibrosis [18,19]. Therefore, inhibiting the early apoptosis of renal tubular cells may prevent the multiple signals from occurring, and thus inhibit the development of fibrosis. The present work aimed to explore new molecular mechanisms, hoping to achieve kidney protection by regulating apoptosis in renal tubular epithelial cells.
The Niban protein encoded by the FAM129A gene is expressed in various cancers and can inhibit apoptosis [20,21]. Increasing evidence indicates that Niban can not only regulate cell death signals at the transcriptional level to play an inhibitory role in apoptosis [22], but also suppress p53-dependent apoptosis by reducing the formation of NPM–MDM2 complex [23]. In addition, lowering Niban expression can lead to up-regulation of p21 and induce apoptosis by inhibiting cell cycle and cell proliferation [24]. Studies have also shown that vasomotor dysfunction leads to decreased phosphorylation of anti-apoptotic protein Niban and promotes apoptosis, while recovery and increase of Niban phosphorylation can upregulate the anti-apoptotic protein Akt and reduce caspase 3/7 activities, restoring vasomotor function and inhibiting apoptosis [25]. Further studies demonstrated that Niban is involved in ER stress and induces autophagy by regulating the mTOR pathway to inhibit apoptosis [22,26]. Due to the anti-apoptotic effect of Niban in various cells, we hypothesize whether Niban plays a role in renal fibrosis by regulating HK-2 cells apoptosis. Our previous study found that Niban is reduced in obstructive nephropathy, UUO rats, as well as in TGF-β1-stimulated HK-2 cells; meanwhile, inhibition of Niban induces apoptosis in HK-2 cells, indicating that Niban may be involved in HK-2 cells apoptosis, but the specific mechanism remains unclear [14].
Apoptosis as a process of regulating cell death, is mainly composed of complex intracellular signal networks. There are three different triggering pathways, including the exogenous (classical death receptor), endogenous (subdivided into mitochondria- and ER stress-induced types) and non-caspase-dependent pathways [27–30]. The Fas/FasL pathway is the most classical death receptor pathway and plays an important role in apoptosis [29,31]. As a marker protein of this pathway, the activation of caspase 8 not only directly triggers apoptosis by activating caspase 3, 6, and 7 (type 1 mechanism), but also induces apoptosis indirectly by activating caspase 9 to stimulate the mitochondrial pathway (type 2 mechanism) [32–34].
In vivo experiments, with the development of UUO obstruction, Niban decreased while apoptosis increased in renal tubular epithelial cells. This result is consistent with our previous UUO rat experiment, suggesting that Niban may be involved in renal tubular epithelial cell apoptosis and thus participate in RIF. As the mechanism is still unclear, we have validated and further explored the results of previous studies. We found that Niban is a functional protein which regulates apoptosis. Our results showed that silencing of Niban up-regulated the levels of cleaved-caspase 8, cleaved-caspase 9, α-SMA, and apoptosis, while down-regulated the level of E-cadherin. Overexpression of Niban down-regulated the level of cleaved-caspase 8, cleaved-caspase 9, α-SMA, and apoptosis, while up-regulated the levels of E-cadherin. Among them caspase 8 and caspase 9 are involved in the Fas/FasL pathway and the mitochondrial stress pathway, respectively, and are marker proteins on these two apoptotic pathways [16,31]. These findings indicated that Niban may inhibit apoptosis in HK-2 cells through caspase-dependent pathway, which is mainly related to the Fas/FasL pathway and the mitochondrial stress pathway. In addition, E-cadherin plays an important role in renal fibrosis as an epithelial marker, α-SMA is a marker protein of myofibroblasts, and it is generally believed that myofibroblasts are the main cell type that produces collagen during fibrosis. References showed that increasing the expression of α-SMA and decreasing the expression of E-cadherin may promote fibrosis [35,36]. Our results showed that overexpression of Niban can reduce AngII-induced apoptosis, decreased expression of α-SMA, and increased expression of E-cadherin. Therefore, we speculated that Niban may relieve fibrosis by inhibiting apoptosis, indicating that Niban may become a new potential target for the treatment of renal fibrosis.
ER stress is a response which multiple stimuli induce the activation of an unfolded protein in the body, which leads to the accumulation of unfolded proteins in the cell, and apoptosis occurs when the stimulation is too severe to be restored by the internal environment. Common ER stress inducers include TM [37–39]. Since caspase12 is involved in the ER stress pathway and is one of its molecular markers [17,30], we examined the marker protein cleaved-caspase 12 of the ER stress pathway. Our results showed that there was no change in the expression of cleaved-caspase 12 after AngII-induced injury. We hypothesize that AngII is not a classical ER stress-inducing factor, but a common inducer of apoptosis. Therefore, AngII induced many other possible apoptotic network pathways, which affected the experimental results.
To investigate whether Niban regulates apoptosis in HK-2 cells through the ER stress pathway, TM was used to stimulate HK-2 cells to detect the marker proteins Bip and Chop of ER stress pathway [37]. We found that after the silencing of Niban, the Bip and Chop of HK-2 cells were significantly increased, especially under the synergistic intervention of TM. The same trend was confirmed in UUO mice, where we found that seven days after UUO modeling, the levels of Bip, Chop, and P53 were significantly increased. P53 is a protein that induces apoptosis. When the damage of the gene is too serious to repair, the p53 protein regulates the repair of cellular DNA and induces apoptosis [40]. These results suggested that silencing the expression of Niban can promote the degree of ER stress in HK-2 cells. Our findings are consistent with the results of Zhang et al. [41], who showed that Chop-related ER stress is associated with the development of renal fibrosis in both CKD patients and unilateral ureteral obstruction (UUO)-induced animals. Studies revealed that loss of Chop protected tubular cells from UUO-induced apoptosis, thereby slowing the progression of renal fibrosis. In view of the role of ER stress in the apoptosis of renal tubular epithelial cells, we speculate that Niban may also regulate the apoptosis of renal tubular epithelial cells by affecting the ER stress pathway.
In summary, our previous study found that Niban plays a role in the apoptosis of renal tubular epithelial cells, which may be involved in RIF, but the specific mechanism of action remains unclear. Based on previous studies, we once again confirmed that Niban is involved in the regulation of HK-2 cells apoptosis. We further discovered that Niban inhibits apoptosis through caspase-dependent pathway, not only via the Fas/FasL and mitochondrial stress pathways, but also ER stress pathways. By regulating these apoptosis pathways, Niban ultimately affected fibrosis. However, our current mechanism research is mainly focused on the cells level, and Niban knockout mice can be further verified and discussed in the future.
Ethical approval
Animal experiments conform to internationally accepted standards and have been approved by the Ethics Committee of Central South University Department of Laboratory Animals, permit number is 2018sydw0227.
Funding Statement
This project was supported by the National Natural Science Foundation of China [81600535] and the National Natural Science Foundation of Hunan Province [2016jj4106].
Disclosure statement
No potential conflict of interest was reported by the authors.
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