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. Author manuscript; available in PMC: 2020 May 2.
Published in final edited form as: Chembiochem. 2019 Mar 14;20(9):1133–1138. doi: 10.1002/cbic.201800756

Figure 5.

Figure 5.

Activity of mutant enzymes on full-length histone H3. (A) Schematic showing chemically methylated histone H3 using thialysine approach. (B) ESI-HRMS spectrum of H3KC9me3. (C) Demethylation of full-length H3KC9me3 by N198A mutant as determined by western blot using H3K9me3 antibody (top panel); Histone H3 as loading control (bottom panel). Demethylation was significantly inhibited by the ethyl-NOG 3. (D) Quantitative representation of the western blot data shown in C. Error bars are based on two independent biological replicates.