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. 2019 May 15;24(10):1866. doi: 10.3390/molecules24101866

Figure 5.

Figure 5

The effect of VT on the expression of genes involved in Akt signaling pathway. Insulin receptor substrate 1 (Irs1), RAC-beta serine/threonine-protein kinase (Akt2), mammalian target of rapamycin (Mtor), and regulatory-associated protein of Mtor (Rptor) were examined by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), and the glyceraldehyde-3-phosphate dehydrogenase (Gapdh) gene, as a standard internal reference gene, was simultaneously examined for qRT-PCR normalization. Schematic diagram of the indicators related to the Akt signaling pathway in this study (A); the level of serum insulin in all groups (B); mRNA expression of Irs1 (C), Akt2 (D), Mtor (E) and Rptor (F) in the liver of each group. The data are presented as floating bars (min-max), and the CON group was used to standardize. * p < 0.05, *** p < 0.001 in comparison to the CON group, # p < 0.05, ## p < 0.01, ### p < 0.001 compared to the MOD group, & p < 0.05 contrasted to the MET group, n = 6, three replicates.