In the article “Tenascin-R Is an Intrinsic Autocrine Factor for Oligodendrocyte Differentiation and Promotes Cell Adhesion by a Sulfatide-Mediated Mechanism,” by P. Pesheva, S. Gloor, M. Schachner, and R. Probstmeier, which appeared on pages 4642– 4651 of the June 15, 1997 issue, detail in Figure6A was not maintained in the printing process. The figure and legend are reprinted.
Fig. 6.
Effect of TN-R on TN-R mRNA and protein expression by cultured OLs, as determined by RT-PCR (A) and Western blot analysis of OL-conditioned culture media (B). OLs were maintained for 2 d in vitro on PLL or PLL plus TN-R 160 substrates, and poly(A+) RNA isolated from these cells was analyzed by RT-PCR. As control, GAPDH mRNA expression was analyzed in parallel. The apparent molecular weights of the DNA marker (in bp) are shown at the left margin inA. TN-R released into the culture medium from OLs maintained on PLL (B, lane 1) or PLL plus TN-R 160 (B, lane 2) was immunoprecipitated by using polyclonal antibodies to TN-R as a carrier. Immunoprecipitates and TN-R 160 used for the PLL plus TN-R 160 substrate (B, lane 3) were subjected to SDS-PAGE and Western blot analysis by using monoclonal antibody 596 recognizing both TN-R isoforms. For ECL detection the Tropix Western-Light Plus kit was used according to the manufacturer’s instructions. The apparent molecular weights of the two major TN-R isoforms (in kDa) are shown at the left margin.