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The Journal of Neuroscience logoLink to The Journal of Neuroscience
. 1997 Mar 1;17(5):1616–1624. doi: 10.1523/JNEUROSCI.17-05-01616.1997

Coexpression of Two Odorant-Binding Protein Homologs inDrosophila: Implications for Olfactory Coding

Daria S Hekmat-Scafe 1, R Alexander Steinbrecht 2, John R Carlson 1
PMCID: PMC6573375  PMID: 9030621

Abstract

Odorant-binding proteins (OBPs) are small soluble proteins present in the aqueous medium surrounding olfactory receptor neurons. Their function in olfaction is still unknown: they have been proposed to facilitate the transit of hydrophobic molecules to olfactory receptors, to deactivate the odorant stimulus, and/or to play a role in chemosensory coding. In this study we examine the genomic organization and expression patterns of twoolfactory-specific genes (OS-E and OS-F) ofDrosophila melanogaster, the products of which are members of a protein family in Drosophila sharing sequence similarity with moth OBPs. We show that theOS-E and OS-F transcription units are located <1 kb apart. They are oriented in the same direction and display a similar intron–exon organization. Expression of both OS-E and OS-F proteins is restricted spatially to the ventrolateral region of the Drosophila antenna. Within this region both OS-E and OS-F proteins are expressed within two different types of sensory hairs: in most, if not all, sensilla trichodea and in ∼40% of the interspersed small sensilla basiconica. We consistently observe that OS-E and OS-F are coexpressed, indicating that an individual sensillum can contain more than one odorant-binding protein. The functional significance of the observed expression pattern and its implications for olfactory coding are discussed.

Keywords: odorant-binding protein, Drosophila, sensilla, olfaction, antenna, pheromone-binding protein


In the olfactory systems of terrestrial animals, olfactory receptor neurons extend dendrites into an aqueous phase (vertebrate nasal mucus or insect sensillar lymph) that protects them from desiccation (Farbman, 1992; Hildebrand, 1995). It is not known how hydrophobic odorant molecules traverse these aqueous barriers to reach the odorant receptors. However, both vertebrate olfactory mucus and insect sensillar lymph contain families of small, abundant water-soluble odorant-binding proteins (OBPs) that have been proposed to shuttle hydrophobic odorants to and/or from odorant receptors (Pelosi, 1994; Pelosi and Maida, 1995; Prestwich et al., 1995).

In addition to potentially transporting odorant molecules, OBPs also may play a role in olfactory coding. Multiple related OBPs have been found within the same vertebrate or moth species (Pelosi, 1994; Pelosi and Maida, 1995). These different OBPs may recognize different odorants: two OBPs expressed in the moth Antheraea pernyihave been shown to display different binding preferences for two moth pheromones (Du and Prestwich, 1995; Prestwich et al., 1995). If OBPs with different odorant specificities are restricted to different regions of the olfactory system, they might influence which odorants have access to the underlying olfactory receptors in these regions (Vogt et al., 1991).

Moths contain two classes of OBPs: pheromone-binding proteins (PBPs), which bind pheromones in vitro (Vogt and Riddiford, 1981), and general odorant-binding proteins (GOBPs), the ligands of which are unknown (Breer et al., 1990). PBPs and GOBPs are present at high concentrations in the sensillar lymph surrounding the dendrites of receptor cells tuned to pheromones or general odorants, respectively (Laue et al., 1994; Steinbrecht et al., 1992, 1995).

In Drosophila, a family of genes has been isolated, the predicted products of which share sequence similarity with moth OBPs and the transcripts of which are restricted to various subregions of the olfactory system (McKenna et al., 1994; Pikielny et al., 1994). We have concentrated on two such olfactory-specific genes (OS-Eand OS-F), the products of which are structurally similar (68% amino acid identity). OS-E and OS-Ftranscripts both are observed specifically in the ventrolateral region of the antenna (McKenna et al., 1994; Pikielny et al., 1994). This pattern is similar to the distribution of one class of olfactory sensory hairs, the sensilla trichodea (Venkatesh and Singh, 1984). The ventrolateral region of the Drosophila antenna also contains a number of small sensilla basiconica, although most sensilla basiconica are located in the reciprocal dorsomedial region of the antenna (Venkatesh and Singh, 1984; Stocker, 1994).Drosophila sensilla basiconica respond to general odorants (Siddiqi, 1987), as has been observed in other insects.

In this study, we show that the OS-E and OS-Fgenes are located <1 kb apart and contain a similar intron–exon organization. We demonstrate that expression of both OS-E and OS-F proteins is restricted spatially to two distinct types of olfactory hairs located in the ventrolateral region of the antenna: most, if not all, sensilla trichodea and a subset of the small sensilla basiconica. Most interestingly, our immunocytochemical EM studies reveal that OS-E and OS-F are coexpressed, in the sense that all sensilla examined that express one also express the other. Thus, an individual sensillum can contain more than one odorant-binding protein. This finding has potential implications for the roles of odorant-binding proteins in olfactory coding.

MATERIALS AND METHODS

Mapping the OS-E and OS-F genomic region. The λGE1 and λGE3 clones, which carry OS-Eand OS-F genomic DNA, were isolated (McKenna et al., 1994) from a Canton-S genomic library in λEMBL3 (kindly provided by I. Dawson, Yale University) with an OS-E cDNA probe. Plasmid subclones of portions of these genomic DNAs were prepared as follows: A 4.7 kb HindIII fragment of λGE1 (containingOS-E as well as the first exon of OS-F) was cloned into XbaI-cut pBluescript KS+(Stratagene, La Jolla, CA) to create pDH58; a 1.9 kb SalI fragment of λGE1 (containing the first exon ofOS-F) was cloned into BamHI-cut pBluescript KS+ to create pDH59; and a 12 kbSalI fragment of λGE3 (containing the last three exons ofOS-F) was cloned into BamHI-cut pBluescript KS+ to create pDH60. In all cases, the recessed termini of the vector and insert were partially filled in with Klenow and the first two dNTPs to create cohesive termini, as described (Hung and Wensink, 1984). Restriction mapping of pDH58–60 was performed by standard procedures (Sambrook et al., 1989).

A series of 5′ and 3′ primers was designed to span the OS-Eand OS-F cDNA sequences (McKenna et al., 1994) (GenBank accession numbers U02543 and U02542, respectively). These primers were used to amplify OS-E and OS-F genomic sequences (from pDH58 or pDH59–60, respectively) or cDNA sequences [from pDH50 or FF4 (McKenna et al., 1994), respectively], using PCR. PCR conditions were as follows: denaturation at 94°C for 5 min, followed by 35 cycles of 48°C for 1 min, 72°C for 2 min, and 94°C for 45 sec, and then one cycle of 48°C for 3 min and 72°C for 10 min. PCR reaction products were analyzed by 1.2% agarose/Tris borate EDTA gel electrophoresis. In cases in which PCR amplification of the corresponding genomic and cDNA clones produced fragments of identical size, we concluded that no intron was present in the corresponding stretch of genomic DNA. When the PCR product produced from a genomic template was larger than one made from the cDNA template, we concluded that one or more introns were present in the stretch of genomic DNA. Both strands of the corresponding stretch of genomic DNA were subjected to dideoxynucleotide sequence analysis with the appropriate PCR primers and a Sequenase 2.0 kit (Stratagene), according to the manufacturer’s directions. In each case, the intron insertion site was determined from both directions. Additional genomic sequence analysis of portions of the long 5′ untranslated region of OS-F (present in pDH59) was performed to ensure that no additional introns were present. Sequence analysis of the OS-E and OS-F 5′ upstream regions in pDH58 and pDH60, respectively, was performed in an analogous manner. Additional sequencing of the third OS-Fintron and the intervening region between OS-E andOS-F was done by the W. M. Keck Foundation Biotechnology Resource Laboratory at Yale University. The OS-E andOS-F genomic sequences have been submitted to GenBank (U81502 and U81503, respectively).

Two bacteriophage P1 (DS00431 and DS06666) carrying ∼80 kb inserts ofDrosophila genomic DNA (Smoller et al., 1991), which cross-hybridized to a hexamer-labeled (Feinberg and Vogelstein, 1983)OS-F probe, were identified by Southern hybridization (Sambrook et al., 1989). Bacteriophage DNAs were prepared as described (Pierce and Sternberg, 1992) from a series of isolates of theDrosophila P1 library known to map to chromosomal region 83CD (Berkeley Drosophila Genome Project) and were bound to nitrocellulose with a Hybri-Slot filtration manifold (BRL, Bethesda, MD), essentially as described by the manufacturer. The position and orientation of the OS-E and OS-F genomic locus on a deduced restriction map encompassing DS06666 and a portion of DS00431 were used to determine the orientation of the OS-E andOS-F locus relative to the centromere of Chromosome III.

Production and purification of His-tagged OS-E and OS-F proteins.A DNA fragment corresponding to mature OS-E carrying a 6xHis tag at its C terminus was produced by PCR amplification of pDH50 (McKenna et al., 1994) with the 5′ primer E9 (5′ GGG ATT CCA TAT GCA GGA ACC AAG GCG CGA TGG) and the 3′ primer E5 (5′ GCT CTA GAT TAA TGG TGA TGG TGA TGG TGG ACC AAA AAG TAG TGG ACA GG). One corresponding to mature OS-F carrying a 6xHis tag at its C terminus was produced by PCR amplification of FF4 (McKenna et al., 1994) with the 5′ primer F7 (5′ GGA ATT CCA TAT GCT GAT CCT GCC GCC GGC TGC) and the 3′ primer F4 (5′ GCT CTA GAT TAA TGG TGA TGG TGA TGG TGC GGC AAG AAG TAG TGC TTG G). Vent DNA polymerase (New England Biolabs, Beverly, MA) was used as recommended by the manufacturer. PCR conditions were as follows: denaturation at 94°C for 5 min, followed by 15 cycles of 55°C for 45 sec, 72°C for 1 min, and 94°C for 45 sec, and then one cycle of 55°C for 45 sec and 72°C for 5 min. TaggedOS-E and OS-F fragments were subcloned into the T7-SS expression plasmid (a kind gift of S. J. Smerdon, Yale University) to create pDH97 and pDH98, respectively.

Cultures of Escherichia coli strain BL21 (λDE3) carrying either pDH97 or pDH98 were harvested after 3 hr of induction with 4 mm isopropyl thiogalactoside (IPTG). Frozen cell pellets were resuspended in 10 ml of buffer A (6 mm guanidinium HCl, 0.1 m Na-phosphate, and 0.01 m Tris, pH 8.0) containing 10 mm β-mercaptoethanol (β-ME), and His-tagged OS-E and OS-F proteins were isolated on Ni-NTA resin (Qiagen, Hilden, Germany) under denaturing conditions as recommended by the manufacturer, except that a batch procedure (Nonet et al., 1993) was used. Proteins were renatured under oxidizing conditions used successfully for Antheraea polyphemus PBP (Prestwich, 1993), except that dialysis was concurrent with renaturation. Briefly, 5.5 ml of each eluate was brought to 14.5 mm cysteine, pH 8, and incubated on a room temperature rotator for 10 min to oxidize the residual β-ME. Then the resulting samples were dialyzed overnight at 4°C in 0.1 m Tris, 5 mm cysteine, and 0.02% sodium azide (NaN3), pH 8. The samples were concentrated to ∼1 mg/ml in PBS, pH 8, using Centriprep-10 filters (Amicon, Beverly, MA), as described by the manufacturer.

Production and affinity purification of anti-E and anti-F antisera. For production of each antiserum, four mice were immunized with 50 μg of the corresponding His-tagged protein, boosted with an additional 50 μg of protein after 3 and 5 weeks, and then boosted with 10 μg of protein every 4 weeks thereafter. The protein preparations were mixed with equal volumes of Complete Freund’s adjuvant for the initial immunizations and of Incomplete Freund’s adjuvant for the subsequent boosts. Aliquots (100 μl) of anti-E and anti-F antisera were affinity-purified on 100 μg of the corresponding His-tagged protein coupled to Reacti-Gel 6x beads (Pierce) in 0.1m Na-borate, pH 8.5, as indicated by the manufacturer. The affinity purification procedure of Harlow and Lane (1988) was adapted for a microfuge tube; all steps were performed at room temperature. Each serum sample was diluted fivefold with 10 mm Tris, pH 7.5, and incubated with the corresponding beads for 45 min on a rotator, compacted (∼0.5 min at 500 × g), and washed with 10 mm Tris, pH 7.5, and then with 10 mmTris, pH 7.5/0.5 mm NaCl. Bound protein was eluted by a 15 min incubation with 0.1 m glycine, pH 2.5 (corresponding to 2.5× the original volume of serum), and the eluate was combined immediately with an equal volume of 1 m Tris, pH 8.0, 100 μg/ml bovine serum albumin, and 0.02% NaN3.

For preparation of subtracted anti-E antibody, the primary anti-E serum (diluted fivefold with 10 mm Tris, pH 7.5) was added to OS-F-coupled beads and incubated on a rotator for 1.5 hr at room temperature. Then the beads were compacted and the unbound antibody fraction applied to OS-E-coupled beads and affinity-purified as described above, except that bound protein was eluted with a volume of 0.1 m glycine, pH 2.5, which corresponded to 12.5× the original volume of serum. The reciprocal purification scheme was used to prepare the subtracted anti-F antibody. All affinity-purified antibodies were stored at 4°C.

Western analysis and immunolocalization. TheDrosophila strain used for all immunological analyses, D222, is a derivative of Canton S-5 (Helfand and Carlson, 1989) made isogenic for Chromosome III (where OS-E and OS-F are located). Drosophila antennae were collected from equal numbers of males and females after immersion in liquid nitrogen as described by Störtkuhl et al. (1994). SDS-PAGE sample buffer (Sambrook et al., 1989) was added directly to the frozen antennae, the sample heated to 95°C for 10 min, and the sample microfuged at top speed for 10 min to pellet cellular debris.

SDS-PAGE and Western transfer to BA83 nitrocellulose (Schleicher & Schuell, Keene, NH) were done by standard methods (Sambrook et al., 1989). Protein was detected with a Western light chemiluminescent detection system (Tropix, Bedford, MA) essentially as described by the manufacturer, except that blocking was done overnight at 4°C. Preimmune sera were diluted 1:1000, the 5× more dilute affinity-purified antisera 1:200, and the 25× more dilute subtracted antisera 1:40.

Immunohistochemistry on Drosophila head sections was performed as described by Raha and Carlson (1994), except that tissue fixation was accomplished by a 1 min incubation in Histochoice (AMRESCO, Solon, OH), and detection was performed with a Vectastain ABC elite kit (Vector Labs, Burlingame, Ca) essentially as described by the manufacturer. Preimmune sera were diluted 1:2000 and affinity-purified antisera, 1:200. The secondary antibody, biotinylated anti-mouse IgG (Vector Labs), was diluted 1:300.

For immunocytochemistry at the electron microscopic level,Drosophila antennae and maxillary palps were cryofixed by plunging the heads with the attached appendages into super-cooled propane at −180°C. Then specimens were freeze-substituted in acetone (pure or containing 3% glutaraldehyde) at −80°C, embedded in LR White resin (London Resin) at room temperature and polymerized at 60°C (Steinbrecht, 1993). Ultrathin sections were cut with a diamond knife on a Reichert OmU2 ultramicrotome and picked up on Formvar-coated single-hole grids. The affinity-purified anti-E and anti-F antisera were diluted from 1:200 to 1:1000; the corresponding subtracted antibodies were diluted from 1:60 to 1:200. Preimmune sera, used as control, were diluted from 1:200 to 1:10000. Goat anti-mouse IgG conjugated with 10 nm of colloidal gold (BioCell, Cardiff, UK) was used as the secondary antibody and was diluted 1:20. Silver intensification (Danscher, 1981) enlarged the grains to ∼40 nm. Further details of the immunocytochemical protocol are described by Steinbrecht et al. (1995).

RESULTS

OS-E and OS-F genes are located in close proximity to each other

A detailed analysis of the genomic region encompassing OS-Eand OS-F is shown in Figure1A. The OS-E andOS-F transcription units are 0.64 and 3.7 kb, respectively. The two transcription units are oriented in the same direction ∼930 bp apart (Fig. 1A). Two small introns are present in the OS-E coding region: one of 62 bp between E24and W25 and another of 50 bp between D49 and E50 (Fig. 1B). OS-F has three introns in its coding region: one of 79 bp between N38 and Y39, one of 429 bp between E63 and A64, and one of 54 bp between K149 and H150. OS-F has a fourth intron of ∼2.2 kb in its 5′ noncoding region. The two introns in OS-E are present at locations identical to those of two of the introns inOS-F (Fig. 1B).

Fig. 1.

Fig. 1.

Genomic organization of OS-E andOS-F genes. A, Genomic organization of the OS-E and OS-F transcription units. Both genes are transcribed from left toright, as indicated by the arrows. Exons of cDNAs are indicated by thick boxes, and introns are shown as thin lines below these boxes. Coding sequences are shown as striated boxes; noncoding sequences are indicated by solid boxes.B, Sequence of a portion of OS-E andOS-F showing the location of two introns found at identical positions in each gene. The sequence alignment betweenOS-E and OS-F corresponds to amino acids 24–51 of OS-E and 38–65 of OS-F. The first of six conserved cysteine residues is boxed. Intron sizes and insertion sites (arrowheads) are indicated. Note that the two introns in OS-E (62 and 50 bp in size) are located at positions corresponding to those of two introns in OS-F (79 and 429 bp in size).

Genomic DNA upstream of the presumptive OS-E andOS-F transcriptional start sites, as determined by 5′ reverse transcriptase amplification of cDNA (McKenna et al., 1994;Pikielny et al., 1994), revealed a TATAAA sequence at −29 relative to the 5′ end of the OS-F cDNA. Although no clear TATA box was seen in theOS-E upstream DNA, the sequence ATAAAA was present at −32 from the 5′ end of the cDNA. Analysis of several hundred nucleotides of genomic DNA sequence upstream of OS-E and OS-Fdoes not reveal extensive sequence similarities between the upstream regions of the two genes. However, the 225 bp upstream of theOS-E 5′ end contains five precise repeats and one variant repeat (containing one different nucleotide) of the octamer PyCATTTPuPy (data not shown), which may represent a repeated enhancer motif. The 425 bp upstream of the OS-F 5′ end contains three precise and three variant repeats of the related heptamer CATTTPuPy (data not shown).

Low-stringency Southern hybridization of EcoRI,HindIII, BamHI, or XbaI-digested genomic DNA with either an OS-E or OS-F cDNA probe revealed only the expected OS-E and OS-F fragments (data not shown), suggesting that there are no other genes closely related toOS-E/OS-F either in this cluster or elsewhere in theDrosophila genome.

OS-E and OS-F are found in two types of sensilla

Recombinant bacterially expressed proteins corresponding to mature OS-E or OS-F (each with a “6xHis tag” of six Histidine residues at the C terminus) were used to generate polyclonal anti-E and anti-F antisera in mice. Western analysis revealed that affinity-purified anti-E and anti-F antisera both recognize small proteins expressed in antennae, but not heads (Fig. 2). The sizes of the antennal proteins detected with the anti-E and anti-F antisera were 14.5 and 13.5, respectively, which are in reasonable agreement with those expected for mature OS-E and OS-F—14.4 and 14.1 kDa, respectively (McKenna et al., 1994). Neither preimmune serum demonstrates discernible reactivity with proteins present in either the antennal or head extract. Male and female antennae showed no significant differences in the sizes or abundance of OS-E and OS-F proteins (data not shown).

Fig. 2.

Fig. 2.

Western analysis of OS-E and OS-F proteins inDrosophila antennae and heads. Body parts were collected from equal numbers of male and female Drosophila. Fractions of the resulting extracts corresponding to 25 antennae (A) or 1½ heads depleted of antennae (H) were subjected to 15% SDS-PAGE and transferred to nitrocellulose. Primary antisera were either affinity-purified anti-OS-E (α-E), affinity-purified anti-OS-F (α-F), or preimmune sera from the corresponding mice. Positions of molecular weight markers are indicated on the right.

Immunohistochemical analysis with the affinity-purified anti-E and anti-F antisera shows labeling in the lateral and ventral region of the antenna (Fig. 3A), where their transcripts are also present (McKenna et al., 1994). Labeling often was found beneath individual sensilla trichodea (Fig. 3B) and, occasionally, sensilla basiconica (data not shown). Although the resolution of this analysis does not allow a precise identification of the cells labeled, we note that the location of the label directly beneath the cuticle corresponds to the position of the sensillar auxiliary cells and the sensillum lymph cavities; neuronal cell bodies are located further below the cuticle (R. A. S., unpublished observations). In some cases staining extends into the sensory hair itself (Fig. 3B). No discernible staining was observed in the brain or other chemosensory organs (maxillary palps and proboscis) with either the anti-E or anti-F antiserum (Fig. 3C and data not shown). The anti-F antiserum did show some staining in the eye. Because no OS-F transcript is detectable in the eye by RNAin situ hybridization (McKenna et al., 1994) nor OS-F protein in heads by Western (Fig. 2), this staining most likely reflects nonspecific labeling.

Fig. 3.

Fig. 3.

Immunohistochemical staining of OS-E and OS-F inDrosophila antennae. A, Representative immunohistochemical staining of a Drosophila antenna with affinity-purified α-E (1:200). The antenna is oriented such that lateral is right and ventral is down. Strong staining is observed beneath the cuticle in the lateral portion of the antenna. The strongest staining is in the region whose boundaries are indicated by arrows; weaker staining is observed in the area indicated by the dotted lines. Magnification is 200×. B, Representative immunohistochemical staining of a Drosophila antenna with affinity-purified α-F (1:200). Again, lateral isright and ventral is down. Staining observed beneath three individual sensilla trichodea is indicated byarrows. Magnification is 400×. C, Immunohistochemical staining of a Drosophila head section with affinity-purified α-E (1:200). The right half of the head is shown such that lateral is right and ventral isdown. Note the absence of discernible specific staining in the brain (left, center totop) and eye (right). Magnification is 50×.

The sensillar and subcellular distribution of OS-E and OS-F proteins was examined at higher resolution with immunocytochemical electron microscopy. In the electron microscope, sensilla trichodea can be distinguished from sensilla basiconica by the thicker cuticular wall of the sensilla trichodea. In addition, sensilla trichodea have essentially unbranched outer dendritic segments and a smaller number of pores per unit of surface area, as compared with the sensilla basiconica, which display branched dendrites and a high pore density (Venkatesh and Singh, 1984; R. A. S., unpublished observations). However, on a single section this discrimination may not always be unequivocal, because the sensilla trichodea display progressively thinner walls and some dendritic branching toward the tip. Hence, accurate assessment of sensillar type requires a reconstruction of a series of sections through the same sensillum. Another potential complication is that intermediate sensillar types have been observed in other insect species and most likely occur in Drosophila as well (Steinbrecht, 1996a).

Sensilla that were labeled by anti-E or anti-F antibodies displayed the highest labeling density over the extracellular sensillum lymph in the lumen of the sensory hair as well as in the sensillum lymph cavity below the base of the hair (Fig.4A–C). We did not observe labeling of either dendrites or cell bodies of olfactory receptor neurons. Some intracellular labeling was found in the sensillar auxiliary cells (Fig.4B). Neither epidermal cells nor hemolymph was labeled. The cuticle often showed some nonspecific background, which also was observed when preimmune serum was substituted for affinity-purified antibody.

Fig. 4.

Fig. 4.

Immunocytochemical localization of OS-E and OS-F in Drosophila olfactory sensilla. A, Longitudinal section through the base of a sensillum trichodeum labeled with α-F (1:1000). The sensillum lymph in the hair lumen and in the cavity below the hair base is strongly labeled. P, Pore;D, dendrite, C, cuticle.B, Cross section of an olfactory sensillum below the hair base labeled with α-E (1:200). Most gold grains are found over the extracellular sensillum lymph (SL) between the microvilli and microlamellae of the auxiliary cells, but there is also some intracellular labeling in the tormogen cell (To). Part of another labeled sensillum is seen in thebottom right corner. Dendrites (D) and epidermal cells (E) are not labeled. The few grains found over the cuticle (C) represent nonspecific background. C, Representative cross section through the distal portion of the third antennal segment after immunolabeling with α-E (1:200). A sensillum trichodeum (T) and three unspecified sensilla (•) are labeled, showing a high density of gold grains. A sensillum basiconicum (B), a sensillum coeloconicum (Co), and two unspecified sensilla (O) are not labeled. The few scattered gold grains observed in nonlabeled areas represent nonspecific background.

The immunolabeled sections revealed no differences between males and females, and the data from both sexes were, therefore, pooled. OS-E and OS-F were labeled in all typical sensilla trichodea (n= 102) and in 8 of 10 intermediate cases in which discrimination between sensilla trichodea and basiconica was uncertain. A fraction of the small sensilla basiconica, which are found intermingled between the sensilla trichodea on the ventrolateral portion of the antenna, also was labeled (15 of 34 sensilla examined). The labeling density on these sensilla was consistently lower than on the sensilla trichodea (Fig.5). No labeling was observed in the large sensilla basiconica (n = 24), which are found primarily in the dorsomedial portion of the antenna, nor in hairs of another class, the sensilla coeloconica (n = 17).

Fig. 5.

Fig. 5.

Representative immunocytochemical labeling of two classes of olfactory sensilla with subtracted antiserum against OS-E (1:60). Two sensilla trichodea (T) are strongly labeled; a sensillum basiconicum (B) shows weaker labeling (reduced density of gold grains). In the bottom left corner, part of another labeled sensillum is visible.S, Spinule (noninnervated microtrichium).

OS-E and OS-F colocalize in individual sensilla

Antisera that are specific for OS-E or OS-F were produced by removing the fraction of one antiserum that bound to the second protein (cross-linked to beads), followed by affinity purification of the subtracted antiserum on beads containing the first protein (Fig.6A). The antisera were specific for either OS-E or OS-F, respectively, on Western blots of extracts from bacteria expressing OS-E or OS-F (Fig. 6B). We noted the presence of faintly labeled dimer-sized bands of ∼30 kDa in extracts prepared from bacteria expressing either OS-E or OS-F.

Fig. 6.

Fig. 6.

Subtracted anti-OS-E and anti-OS-F antisera are specific. A, Preparation of the subtracted anti-OS-E antiserum (α-E). (The reciprocal procedure was used to prepare subtracted anti-OS-F antiserum.) Briefly, the primary anti-OS-E antiserum was incubated with purified OS-F (conjugated to beads) to deplete it of antibodies that also recognize OS-F. Then the depleted anti-OS-E antiserum was affinity-purified by binding to purified OS-E (conjugated to beads) and then by subsequent elution. B, Western analysis showing that the subtracted anti-OS-E and anti-OS-F antisera are each specific for the corresponding purified protein. 0.1 μg of OS-E (E) and OS-F (F) protein, purified from E. coli, were subjected to 15% SDS-PAGE and transferred to nitrocellulose. The subtracted affinity-purified α-E (left) or α-F (right) antiserum served as the primary antibody. Positions of molecular weight markers are indicated on theright. Note the presence of a faint dimer-sized band of ∼30 kDa in the extreme left lane.

Using these subtracted affinity-purified antisera on alternating sections (Fig. 7A,B), we were able to check for the presence of both OS-E and OS-F in a series of individual sensilla. Whenever a sensillum was labeled by subtracted anti-OS-E, it also was labeled by anti-OS-F, and vice versa (n = 113 sensilla). There were also a number of sensilla that were not labeled by either antiserum, in particular those in the dorsomedial region of the antenna. The simplest interpretation of these results is that at least some individual sensilla contain more than one odorant-binding protein.

Fig. 7.

Fig. 7.

Immunocytochemical demonstration of OS-E and OS-F colocalization in olfactory hairs. A andB represent sections (∼0.4 μm apart) of a section series through the same two sensilla. A is labeled by subtracted α-F (1:60), whereasB is labeled by subtracted α-E (1:60). In both sections, the sensillum trichodeum (T) is strongly labeled, whereas the sensillum basiconicum (B) remains unlabeled. S, Spinule.

DISCUSSION

In this article we present evidence that OS-E andOS-F, two members of a Drosophila family of genes that encode proteins structurally related to moth OBPs, are located <1 kb apart and are coordinately expressed. We find that both OS-E and OS-F proteins are expressed in the same region of theDrosophila antenna and that both are present within two distinct classes of olfactory sensilla. Moreover, we consistently observe coexpression of OS-F in the same sensilla that express OS-E. This is the first demonstration of colocalization of two different OBPs within the same olfactory sensilla.

The localization of OS-E and OS-F proteins is consistent with the notion that they represent Drosophila homologs of moth OBPs. Both OS-E and OS-F are present in the sensillum lymph of sensory hairs housing olfactory receptor neurons as well as in the underlying auxiliary cells. Most likely, OS-E and OS-F are soluble proteins synthesized by the sensillar auxiliary cells, which then secrete them into the sensillar lymph surrounding the olfactory receptor neurons, as also is believed to be the case for the moth OBPs (Steinbrecht et al., 1992, 1995; Laue et al., 1994).

Expression of OS-E and OS-F is restricted to sensilla located in the ventrolateral region of the antenna. Within this region two sensillar types contain OS-E and OS-F proteins: most, if not all, sensilla trichodea and ∼40% of the interspersed small sensilla basiconica. We do not know whether the proteins have an identical function in the two sensillar types. However, expression of OS-E and OS-F may reflect a similar functional specialization of the sensilla that express them. In moths, different functional classes of sensilla express different classes of OBPs. The pheromone-binding proteins are associated with pheromone-sensitive sensilla—primarily the specialized sensilla trichodea of the males (Steinbrecht et al., 1992)—whereas another group of OBPs, termed general odorant-binding proteins, is associated with sensilla that respond to general odorants—primarily the sensilla basiconica present in males and females (Laue et al., 1994; Steinbrecht et al., 1995). This distinction between PBP or GOBP expression seems to reflect a difference in sensillar function rather than simply a property of the morphological type. In Bombyx mori, PBPs are expressed in the sensilla trichodea of males, which respond to female pheromones, whereas GOBPs are expressed in the sensilla trichodea of females, which in this species respond to general plant odors (Steinbrecht et al., 1995).

The coexpression of different Drosophila OBPs (such as OS-E and OS-F) in subsets of sensory hairs could be important for the coding of olfactory information. Different moth OBPs can bind preferentially to distinct odorants (De Kramer and Hemberger, 1987; Du and Prestwich, 1995). Also, comparative immunocytochemical studies suggest a role for OBPs in olfactory coding (Steinbrecht, 1996b). Unlike the olfactory receptor cells of vertebrates, which project into a common OBP-filled mucus layer (Pevsner et al., 1988; Pevsner and Snyder, 1990), those of insects are compartmentalized into separate sensilla with distinct sensillum–lymph cavities. Expression of different combinations of OBPs within these various sensilla thus could influence which odorants have access to the enclosed olfactory receptor neurons. The coexpression of OS-E and OS-F within the same sensillum potentially could broaden the range of odorants to which the olfactory receptor neurons can respond. Different Drosophila sensilla may contain different combinations of the various OBP family members. In this regard, we note that the OS-E and OS-F proteins may not be the only OBPs expressed in the sensilla we examined. For example, there is at least one other OBP gene, PBPRP-1, the transcripts of which also are expressed in the ventrolateral region of the antenna (Pikielny et al., 1994) and which well may be expressed in a subset of the sensilla that express OS-E and OS-F. We note the formal possibility that the subtracted anti-E and anti-F antisera, which appear specific on Western blots (Fig. 6B) do cross-react to some degree in immuno–EM experiments. However, this is unlikely, because then we should have observed reciprocal differences in the intensity of the labeling of different hairs with the two antisera.

Another means of combinatorial coding suggested by our results is the possibility that OBPs coexpressed within a single sensillum could multimerize. We note that faint dimer-sized OS-E and OS-F bands are observed consistently in extracts prepared from bacteria that overexpress one or the other protein (Fig. 6B). If OS-E and OS-F are capable of dimerization, three distinct dimers (E-E, F-F, and E-F) and two monomers potentially could be present, each of which might have a different odorant speci- ficity. If different heterodimers transport different odorants to receptors, the possibility for heterodimer formation among a variety of OBPs that are expressed in overlapping sets of sensory hairs might provide a means of combinatorial coding of olfactory information.

The close proximity, similar sequences, common orientation, and shared intron positions of the OS-E and OS-F genes suggest that the two genes arose by a gene duplication event. The two additional introns in OS-F might have arisen after such a duplication event. The coordinated expression of OS-E and OS-F proteins may result from the close proximity of the OS-E andOS-F genes. OS-E and OS-F likely share enhancer elements, some of which may have been duplicated during the initial expansion. The other presumptive Drosophila OBP genes are located at different chromosomal locations (Pikielny et al., 1994), and it is unknown whether their intron–exon organization resembles that of OS-E and OS-F. InCaenorhabditis elegans (Troemel et al., 1995) and mouse (Sullivan et al., 1996), families of genes that encode chemosensory G-protein-coupled receptors are also present in clusters. However, genes within a single cluster are not regulated coordinately, because they are expressed in different chemosensory neurons (Troemel et al., 1995; Sullivan et al., 1996).

The close proximity of the OS-E and OS-F genes may present the opportunity to manipulate both genes simultaneously via a variety of genetic and molecular means (Ashburner, 1989). Electrophysiological or behavioral analyses of the odorant responses of the resulting Drosophila strains may be useful in addressing the function(s) of OS-E and OS-F proteins. It also may be possible to compare electrophysiologically the odorant responses of individual trichoid and basiconic sensilla that express altered levels of OS-E and OS-F via single-unit recordings (Siddiqi, 1987; P. Clyne and J. Carlson, unpublished data). Such experiments may provide additional insight into the roles of OBPs in olfactory signal transduction and chemosensory coding.

Footnotes

This work was supported by a National Institute of Deafness and Other Communication Disorders grant to J.C. (R01 DC02174-10) and a research service award to D.H. (DC00139). We are grateful to Barbara Müller for her skillful assistance at the immunocytochemical studies. We thank Michael McKenna, Charles Scafe, and Michael Laue for helpful discussions.

Correspondence should be addressed to Dr. Carlson at the above address.

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