Abstract
Phosphorylation of brain Na+ channel α subunits by cAMP-dependent protein kinase (PKA) decreases peak Na+ current in cultured brain neurons and in mammalian cells and Xenopus oocytes expressing cloned brain Na+ channels. We have studied PKA regulation of Na+ channel function by activation of D1-like dopamine receptors in acutely isolated hippocampal neurons using whole-cell voltage-clamp recording techniques. The D1 agonist SKF 81297 reversibly reduced peak Na+ current in a concentration-dependent manner. No changes in the voltage dependence or kinetics of activation or inactivation were observed. This effect was mediated by PKA, as it was mimicked by application of the PKA activator Sp-5,6-dichloro-1-β-d-ribofuranosylbenzimidazole-3′,5′-monophosphorothioate(cBIMPS) and was inhibited by the specific PKA inhibitor peptide PKAI5–24. cBIMPS had similar effects on type IIA brain Na+ channel α subunits expressed in tsA-201 cells, but no effect was observed on a mutant Na+ channel α subunit in which serine residues in five PKA phosphorylation sites in the intracellular loop connecting domains I and II (LI–II) had been replaced by alanine. A single mutation, S573A, similarly eliminated cBIMPS modulation. Thus, activation of D1-like dopamine receptors results in PKA-dependent phosphorylation of specific sites in LI–II of the Na+channel α subunit, causing a reduction in Na+current. Such modulation is expected to exert a profound influence on overall neuronal excitability. Dopaminergic input to the hippocampus from the mesocorticolimbic system may exert this influence in vivo.
Keywords: Na+ current, neuromodulation, cAMP-dependent protein kinase, hippocampus, dopamine receptors, phosphorylation
Voltage-dependent Na+ current is the primary inward current underlying excitability in the hippocampus and throughout the CNS but is not often recognized as a target for neuromodulation. The neuronal Na+ channel α subunit is phosphorylated by cAMP-dependent protein kinase (PKA) (Costa et al., 1982; Costa and Catterall, 1984; Rossie and Catterall, 1987) on a family of sites in the intracellular loop connecting domains I and II (Rossie et al., 1987; Rossie and Catterall, 1989; Murphy et al., 1993). Activation of PKA reduces Na+ flux in synaptosomes (Costa and Catterall, 1984) and peak Na+ current in cultured rat brain neurons (Li et al., 1992). PKA also reduces current through type IIA brain Na+ channels heterologously expressed in mammalian cells (Li et al., 1992, 1993) or in Xenopusoocytes (Gershon et al., 1992; Smith and Goldin, 1996). These results suggest that Na+ channels might be modulated by neurotransmitters coupled to signaling cascades that alter PKA activity.
Neuronal release of dopamine is a primary modulator of PKA activity in the CNS. In particular, the hippocampus receives a rich dopaminergic innervation from the mesocorticolimbic dopamine system (Meador-Woodruff et al., 1994). Five dopamine receptor subtypes (D1–D5) have been identified (Sibley and Monsma, 1992; Civelli et al., 1993; O’Dowd, 1993), and all are expressed in the hippocampus (Meador-Woodruff et al., 1994). These may be broadly classified as D1-like (D1 and D5) or D2-like (D2–D4) (Stoof and Kebabian, 1981, 1984; Sibley and Monsma, 1992; O’Dowd, 1993). Both classes of receptor couple to adenylate cyclase, but with antagonistic effects. D1-like receptors stimulate adenylate cyclase activity, whereas D2-like receptors inhibit it (Stoof and Kebabian, 1981). Thus, activation of D1-like dopamine receptors could modulate Na+ currents by activating PKA and phosphorylating the Na+ channel α subunit in hippocampal pyramidal neurons. Activation of dopamine receptors reduces peak Na+ current in rat neostriatal neurons through a PKA-dependent pathway (Surmeier et al., 1992; Schiffmann et al., 1995); however, no neurotransmitter receptor has been identified that activates PKA resulting in modulation of Na+ channel activity in hippocampal pyramidal neurons.
We have examined modulation of Na+ currents by D1-like dopamine receptors in acutely isolated rat hippocampal pyramidal neurons. Activation of D1-like dopamine receptors rapidly and reversibly reduces peak Na+ current with no effect on the voltage dependence or kinetics of activation or inactivation. This effect requires the activation of PKA and is mimicked by the extracellular application of direct activators of PKA. Furthermore, we show that the modulation requires phosphorylation of serine 573 in the intracellular loop connecting homologous domains I and II (LI–II) of the Na+ channel α subunit. Dopaminergic input to the hippocampus from the mesocorticolimbic system may activate this pathway in vivo, thus contributing to the regulation of excitability in the hippocampus. A preliminary report of these results has been presented (Cantrell et al., 1996b).
MATERIALS AND METHODS
Acute dissociation of hippocampal neurons.Hippocampal neurons from adult (>25 d postnatal) male rats were acutely isolated using procedures described previously (Bargas et al., 1995; Howe and Surmeier, 1995; Cantrell et al., 1996a). In brief, rats were decapitated under metofane anesthesia. Brains were quickly removed, iced, and blocked before slicing. Slices (400–500 μm) were cut and transferred to a low-calcium (100 μm), HEPES-buffered saline solution containing (in mm): 140 sodium isethionate, 2 KCl, 4 MgCl2, 0.1 CaCl2, 23 glucose, and 15 HEPES, pH 7.4, 300–305 mOsm/l. All solutions were bubbled with 100% O2 before slicing. Slices were then incubated for 1–6 hr in NaHCO3-buffered Earle’s balanced salt solution (Sigma, St. Louis, MO) bubbled with 95% O2 and 5% CO2, pH 7.4, 300–305 mOsm/l. Single slices were transferred to the low-calcium buffer. With the aid of a dissecting microscope, portions of the hippocampus were dissected and placed in a treatment chamber containing protease type XIV (Sigma; 0.7 mg/ml) in HEPES-buffered HBSS (Sigma) at 35°C, pH 7.4, 300–305 mOsm/l. After 5–10 min of enzyme treatment, the tissue was rinsed several times in the low-calcium buffer and mechanically dissociated using a series of fire-polished Pasteur pipettes. The cell suspension was then plated into a 35 mm tissue culture dish (Corning, Corning, NY) mounted on the stage of an inverted microscope containing 1 ml of HEPES-buffered, phosphate-free HBSS. After allowing the cells to settle (about 5 min), the bathing solution was changed to normal external recording solution.
Mammalian cell transfection. tsA-201 cells, which are human embryonic kidney cells (HEK293 cells) that have been stably transfected with simian virus 40 large tumor antigen (Robert Dubridge, Cell Genesis, Foster City, CA), were used for transfection experiments. tsA-201 cells were maintained in DMEM/F12 medium (Life Technologies, Gaithersburg, MD) supplemented with 10% fetal calf serum (HyClone, Logan, UT), 25 U of penicillin/ml, and 25 μg of streptomycin/ml (Sigma). They were cotransfected with cDNA encoding the human CD8 marker protein (EBO-pCD-leu2; American Type Culture Collection, Rockville, MD) and a plasmid encoding either the wild-type (WT) rat brain type IIA Na+ channel α subunit, a mutant Na+ channel α subunit in which serines in each of the consensus PKA phosphorylation sites in LI–II have been mutated to alanine (PKATOT; designated PKACOMP-A bySmith and Goldin, 1996, 1997), or a mutant Na+channel α subunit in which serine 573 has been mutated to alanine (S573A). Cells were transfected using the calcium phosphate precipitation method as described previously (Margolskee et al., 1993). Successfully transfected cells were then identified with magnetic polystyrene microspheres coated with anti-CD8 antibody (Jurman et al., 1994; M-450 CD8 Dynabeads, Dynal, Great Neck, NY).
Whole-cell voltage-clamp recording. Whole-cell currents were recorded from pyramidally shaped hippocampal neurons that had at most one or two short processes or from tsA-201 cells expressing WT or mutant Na+ channel α subunits (Hamill et al., 1981; Bargas et al., 1995; Howe and Surmeier, 1995). Electrodes were pulled from 75 μl micropipette glass (VWR Scientific, Westchester, PA) and fire-polished before use. A low Na+ extracellular recording solution was used for recording from hippocampal neurons to reduce current magnitude. It contained (in mm): 20 NaCl, 10 HEPES, 1 MgCl2, 0.4 CdCl2, 55 CsCl, 5 BaCl2, and 80 glucose, pH 7.3, with NaOH, 300–305 mOsm/l. For recording from tsA-201 cells, the extracellular solution contained (in mm): 140 NaCl, 10 HEPES, 1 MgCl2, 0.4 CdCl2, 25 CsCl, and 5 BaCl2, pH 7.3, with NaOH, 300–305 mOsm/l. The intracellular recording solution contained (in mm): 189N-methyl d-glucamine, 40 HEPES, 4 MgCl2, 1 NaCl, 0.1 BAPTA, 25 phosphocreatine, 2–4 Na2ATP, 0.2 Na3GTP, and 0.1 leupeptin, pH 7.2, with H2S04, 270–275 mOsm/l. SKF 81297, SKF 38393, and SCH 23390 (Research Biochemicals, Natick, MA) were prepared as concentrated stocks in water and frozen in aliquots before use. Tetrodotoxin (TTX; Calbiochem, San Diego, CA), PKA inhibitor (PKAI) and PKC inhibitor (PKCI) (PKAI5–24 and PKCI19–36, respectively; Peninsula Labs, Belmont, CA) were prepared as concentrated stocks in water and diluted immediately before recording. Sp-5,6-dichloro-1-β-d-ribofuranosylbenzimidazole-3′,5′-monophosphoro-thioate (cBIMPS; BioLog, LaJolla, CA) was prepared as a concentrated stock in DMSO and diluted before use. Appropriate vehicle controls were performed where necessary.
Electrode resistances were typically 3–6 MΩ in the bath. Recordings were obtained using an Axon Instruments (Foster City, CA) 1C patch clamp. Voltage pulses were delivered, and currents were recorded using a personal computer running Basic-FASTLAB software to control an analog-to-digital/digital-to-analog interface (IDA, Indec Systems, Capitola, CA). Series resistance compensation (70–80%) was routinely used.
Pharmacological compounds were applied using a gravity-fed “sewer pipe” system. The array of application capillaries (∼150 mm inner diameter) was positioned a few hundred micrometers away from the cell under study. Solutions were changed by altering the position of the array with a microprocessor-controlled DC motor (Newport-Klinger, Inc., Irvine, CA). Complete solution changes were achieved within 1 sec, as estimated from the rate of block by TTX or the rate of change in reversal potential after a solution change.
Pulse protocols and data analysis. Data were collected using standard voltage step protocols. Conductance (g) was calculated from peak current measured during test pulses to varying voltages according tog(V) = I/(V −Vrev), where I was the current, V was the test pulse voltage, andVrev was the measured reversal potential. The voltage dependence of inactivation was measured using 20-msec-long conditioning pulses to a varying potential followed by a test pulse to −10 mV. Peak test pulse current was plotted as a function of conditioning pulse voltage. Both conductance-voltage and inactivation-voltage curves were fit with a Boltzmann function of the form:
where Vh was the half-activation or inactivation voltage, k was a slope factor, andgmax was the maximum conductance.
Dose–response curves were fit with a Langmuir isotherm of the form:
where Ipeak was the maximum current elicited by the pulse, Isensitive was the blockable current, [M] was the agonist concentration, EC50 was the concentration of agonist producing 50% block,Iresistant was the portion of the current resistant to modulation, and n was usually set to 1 (varyingn between 0.5 and 1.5 did not significantly enhance the fits for the data described here). Least squares curve fitting and statistical analysis were done using Sigma Plot (Jandel Scientific, Corte Madera, CA). Statistics are presented as mean ± SEM. When the sample sizes allowed, box plots of the data were drawn. In the box plot, the median is represented as the central bar of the box, the edges of the box are the interquartiles (technically fourths), and the “whiskers” are lines drawn to the most extreme points in the sample that are not outliers (defined as points beyond interquartile ± 1.5 × interquartile range); outliers are shown as circles or asterisks (Tukey, 1977).
RESULTS
Na+ current in hippocampal neurons is modulated by activation of D1-like dopamine receptors
As an initial step in studying dopaminergic modulation of Na+ channels by phosphorylation, we examined the effect of agonists of D1-like dopamine receptors. Whole-cell Na+ currents were recorded in acutely dissociated hippocampal neurons using solutions that isolated voltage-dependent Na+ current (see Materials and Methods). Under these recording conditions, all of the remaining voltage-dependent current was blocked by the specific Na+ channel blocker TTX (see Cantrell et al., 1996a). Application of the D1-like dopamine receptor agonist SKF 81297 (Arnt et al., 1992) rapidly and reversibly inhibited the whole-cell Na+ current (Fig.1A,B). Significant reductions in current were observed in 73.4% of the cells tested (n = 34). In the responding cells, saturating concentrations of SKF 81297 (5 μm) reduced current an average of 22.2 ± 1.7% (n = 25). The magnitude of the response varied from cell to cell with a range of 7.1 to 36.8%, as indicated by the box plot in Figure 1B,inset. Similar effects were observed for a second agonist, SKF 38393 (Fig. 1C,D). Saturating concentrations of this agonist (5–10 μm) reduced current an average of 26.8 ± 2.8% (n = 10). These results are in good agreement with data previously reported for the modulation of Na+ current by dopamine receptor agonists in the neostriatum (Surmeier et al., 1992; Schiffmann et al., 1995). To confirm that these effects were mediated via D1-like dopamine receptors, the concentration dependence of the effect and its sensitivity to specific antagonists were tested. The effects of SKF 81297 were concentration-dependent, as shown in Figure2A,B. Concentration–response plots for SKF 81297 were fit with a single Langmuir isotherm having a mean IC50 of 825.4 ± 128.8 nm (Fig. 2A; n = 8 independent experiments). Modulation by 5–10 μm SKF 81297 was prevented in the presence of the D1-like dopamine receptor antagonist SCH 23390 (10 μm; Fig. 2C,D). It was recovered after SCH 23390 removal, confirming that the tested cell was responsive to SKF 81297. These results demonstrate that the observed effects were mediated by the activation of D1-like dopamine receptors. Similar effects of SCH 23390 were observed in four other experiments. Taken together, these data confirm that SKF 81297 acts at D1-like dopamine receptors on hippocampal pyramidal neurons to produce a dose-dependent reduction in tetrodotoxin-sensitive, voltage-dependent Na+ current.
Fig. 1.
D1-like dopamine receptor agonists reduce whole-cell Na+ current. A, The absolute value of peak inward Na+ current is plotted as a function of time during the experiment. The cell was exposed to SKF 81297 (5 μm) by rapid perfusion during the period indicated by the solid bar. Test pulses to −20 mV (40 msec long) were applied once every 2 sec from a holding potential of −75 mV. The same pulse protocol was used in subsequent figures unless otherwise indicated. B, Representative current traces obtained under control conditions (1) and in the presence of SKF 81297 (2) recorded at the times indicated in A. Inset, Box plot showing the extent of current reduction in cells that responded to SKF 81297.C, Effect of SKF 38393 (10 μm) on peak inward Na+ current as described in A.D, Representative current traces obtained under control conditions (1) and in the presence of SKF 38393 (2) at the times indicated in C.Inset, Box plot showing the extent of reduction in cells that responded to SKF 38393.
Fig. 2.
Involvement of a D1-like dopamine receptor in the signaling pathway. A, Dose–response curve for SKF 81297 reduction in current. The solid line is a least squares fit of a Langmuir isotherm to the data withKI = 745 nm.Inset, Box plot summarizing similar data from eight independent experiments. B, Representative current traces from the cell in A obtained in control and in the presence of the indicated SKF 81297 concentrations. C, Peak Na+ current as a function of time as described for Figure 1A. The cell was first exposed to 10 μm SCH 23390, then to a mixture of SCH 23390 and 10 μm SKF 81297, and finally to SKF 81297 alone, as indicated by the bars. D, Representative current traces from the experiment shown in C.
Characterization of effects of D1-like dopamine receptor activation on Na+ current properties
The catalytic subunit of PKA was previously found to reduce peak Na+ current in excised patches from cultured neurons with no discernible effects on the voltage dependence or kinetics of activation or inactivation (Li et al., 1992). As a comparison with those results, we tested whether D1-like dopamine receptor agonists altered these properties of the current in hippocampal pyramidal neurons. The time course of Na+ currents was similar before and after application of 5 μm SKF 81297 (Figs.1B, 3A–D), and the reduction in peak Na+ current was comparable at all test potentials (Fig. 3A–E). This indicates that the voltage dependence of activation of the Na+ current was unchanged, as illustrated by the superimposition of current–voltage curves before and after agonist application (Fig.3E). The half-activation voltage and slope factor in control conditions were −44.2 ± 5.3 mV (n = 8) and 5.3 ± 0.3 mV (n = 8), respectively, and −44.9 ± 5.4 mV (n = 8) and 5.5 ± 0.3 mV (n = 8) in the presence of 5 μm SKF 81297 (Fig. 3F,G). Likewise, the voltage dependence of inactivation during 50 msec prepulses was also unaffected, having a half-inactivation voltage of −51.8 ± 1.5 mV (n = 13) and slope factor of 9.2 ± 0.7 mV (n = 13) in control and a half-inactivation voltage of −54.7 ± 1.6 mV and slope factor of 10.8 ± 0.9 mV (n = 13) in the presence of SKF 81297 (n = 13) (Fig. 3F,G). Thus, the effects of D1-like receptor activation occur without dramatic alterations in the voltage-dependent properties of the Na+ channel, consistent with the characteristics of Na+ channel modulation by PKA (Li et al., 1992,1993).
Fig. 3.
Voltage dependence of D1-like dopamine receptor effect on Na+ currents. A–D, Whole-cell Na+ current elicited by test pulses to the indicated potentials from a holding potential of −75 mV in the absence and presence of 5 μm SKF 81297. In each panel, the smaller current was obtained in the presence of SKF 81297.E, Current–voltage relationship for peak Na+ current in control (closed squares) and in the presence of SKF 81297 (closed circles). The SKF 81297 points have been scaled to facilitate comparison of the voltage dependence of the Na+current in the absence and presence of the compound (shaded triangle). F, Boltzmann fits of the activation and inactivation curves for the same cell in control (closed symbols) and in the presence of SKF 81297 (open symbols). G, Box plot showing the range of half-activation and half-inactivation voltages obtained in control and in the presence of SKF 81297.
In previous experiments, the effects of PKA activation on brain Na+ channels were studied by applying the purified catalytic subunit of PKA to the cytoplasmic surface of excised inside-out patches (Li et al., 1992, 1993). To demonstrate that direct activation of PKA produced the same effects as activation of D1-like dopamine receptors in hippocampal neurons, the modulation of Na+ current by the potent and membrane-permeant activator of PKA cBIMPS (Sandberg et al., 1991) was studied. Application of cBIMPS (20–50 μm) rapidly and reversibly reduced Na+ currents in rat hippocampal neurons (Fig. 4A,B). Currents were reduced in all seven cells treated with 50 μmcBIMPS, with a mean reduction of 21.7 ± 4.5%. Consistent with the preceding studies, the reduction in current attributable to activation of PKA occurred without changes in the voltage-dependent properties or kinetics in rat hippocampal neurons.
Fig. 4.
Reduction of Na+ current by PKA activation. A, Peak Na+ currents elicited in control and in the presence of 50 μm cBIMPS plotted as a function of time during the experiment. B, Representative current traces in control (1) and in the presence of cBIMPS (2) recorded at the times indicated in A. Inset, Box plot showing the extent of current reduction in response to cBIMPS.C, Peak Na+ currents plotted as a function of time. The cell was first exposed to 5 μm SKF 81297, then 50 μm cBIMPS, and finally to a mixture of SKF 81297 and cBIMPS as indicated by the bars. The time course was corrected for a 20% rundown in the currents by subtraction of an exponential fit to the currents during control periods.D, Representative current traces in cBIMPS (2) and in the presence of cBIMPS plus SKF 81297 (3) recorded at the times indicated inC.
The physiological effects of D1-like dopamine receptor activation require PKA
To test directly for the involvement of PKA in the signaling pathway mediating the effects of D1-like dopamine receptor activation in hippocampal pyramidal neurons, we have used the specific peptide inhibitor of PKA, PKAI5–24 (Cheng et al., 1986). When PKAI5–24 was included in the pipette solution at a concentration of 10 μm, and 5–7 min were allowed for intracellular dialysis, SKF 81297 no longer modulated whole-cell Na+ current in hippocampal neurons (Fig.5B). Although a slight increase in Na+ current is observed for the cell in Figure 5B, an average of results on six cells showed that SKF 81297 reduced peak current by 5.5 ± 0.7% (p < 0.006; n = 6). During the same series of experiments, cells dialyzed with control internal solutions responded to SKF 81297 treatment with an average Na+ current reduction of 22.1 ± 4.2% (n = 8; Fig. 5A). The loss of SKF 81297 modulation was not attributable to loss of critical cellular components during the dialysis period, because a similar dialysis period was included in the control experiments. As an additional negative control for the effects of intracellular dialysis with a peptide fragment, we repeated the above experiment using the PKC-specific inhibitor peptide PKCI19–36 (House and Kemp, 1987). When PKCI19–36 was added to the pipette solution at a concentration of 2 μm, and 5–7 min were allowed for intracellular dialysis, a protocol sufficient to block Na+ channel modulation by PKC (Cantrell et al., 1996a), 5 μm SKF 81297 still reduced whole-cell Na+ current an average of 28.2 ± 10.5% (n = 6; Fig. 5C). These data demonstrate that the block of SKF 81297 modulation by PKAI5–24 is attributable to the inhibition of PKA and not to nonspecific effects of the peptide itself. Consistent with this result, pre-exposure to 50 μm cBIMPS to activate PKA occluded any further modulation by SKF 81297 (Fig. 4C,D; n = 5). Thus, activation of PKA is required for D1-like dopamine receptor-mediated reduction of Na+ current in hippocampal neurons.
Fig. 5.
Block of D1-like dopaminergic Na+ current modulation by an inhibitor of PKA. Modulation of peak Na+ current by 10 μm SKF 81297 with a control pipette solution (A) and using pipette solutions containing the PKA inhibitor PKAI5–24 (B) or the PKC inhibitor PKCI19–36 (C). Experimental procedures were as described in Figure1A.
Modulation of Na+ current by PKA requires phosphorylation of the Na+ channel α subunit in one or more identified PKA sites in LI–II
The next set of experiments examined the molecular substrate for the PKA phosphorylation initiated by activation of the D1-like dopamine receptor. The α subunit of the neuronal Na+ channel is phosphorylated at up to four sites by PKA in intact neurons (Rossie and Catterall, 1987). Serine residues 573, 610, 623, and 687 are phosphorylated by PKA both in vitro and in intact cells (Rossie et al., 1987; Rossie and Catterall, 1989; Murphy et al., 1993). A mutant channel in which each of these serines plus nearby serine 554 had been mutated to alanine was resistant to the effects of PKA activation when expressed inXenopus oocytes (Smith and Goldin, 1996). Because there may be significant differences between PKA modulation in Xenopusoocytes and mammalian cells, it was important to determine that these same sites were responsible for the modulatory effects of PKA in mammalian cells under the conditions we had used to activate PKA in hippocampal neurons. For these experiments, we used the same mutant type IIA rat brain Na+ channel α subunit in which the serine residues in each of the identified PKA sites had been mutated to alanine (PKATOT; designated PKACOMP-A bySmith and Goldin, 1996). Plasmids containing cDNA encoding either WT or PKATOT mutant rat brain type IIA Na+channel α subunits were transiently expressed in the tsA-201 cell subclone of HEK293 cells. Application of 20 and 50 μmcBIMPS to tsA-201 cells expressing WT type IIA Na+channel α subunits reduced the expressed Na+current in a concentration-dependent manner (Fig.6A,B). The mean reduction by 50 μm cBIMPS in these cells was 21.3 ± 2.3% (n = 25; Fig. 6F). In contrast, the mutant Na+ channel PKATOT was not modulated by cBIMPS application (Fig. 6C,D,F). The mean reduction was 2.7 ± 0.9% (50 μm;n = 16; p < 0.0005, Student’st test) (Fig. 6F). Recent work has indicated that the PKA site centered at serine 573 is required to mediate the effects of PKA on rat brain type IIA Na+channels expressed in Xenopus oocytes (Smith and Goldin, 1997). To test whether the same site is required for PKA modulation when the channel is expressed in mammalian cells, we used a mutant type IIA rat brain Na+ channel α subunit in which serine 573 had been mutated to alanine (S573A). When transiently expressed in tsA-201 cells, mutant S573A channels were also not modulated significantly by cBIMPS application (Fig.6E,F). The mean reduction was 3.9 ± 2.1% (50 μm; n = 11; p< 0.0005, Student’s t test) (Fig. 6F). These experiments demonstrate that PKA-dependent modulation of neuronal Na+ channels expressed in mammalian cells requires phosphorylation of serine 573 of the Na+ channel α subunit.
Fig. 6.
Modulation by PKA activation requires direct phosphorylation of the Na+ channel α subunit.A, tsA-201 cell expressing WT rat brain type IIA α subunits was exposed to cBIMPS (20–50 μm) during the periods indicated by the solid bars. B, Representative current traces obtained under control conditions (1) and in the presence of cBIMPS (2, 3) at the times indicated in A. The absolute value of peak inward Na+ current is plotted as a function of time during the experiment. C, A tsA-201 cell expressing expressing mutant PKATOT was exposed to cBIMPS (20–50 μm) during the periods indicated by thesolid bars. The absolute value of peak inward Na+ current is plotted as a function of time during the experiment. D, Representative current traces obtained under control conditions (1) and in the presence of cBIMPS (2, 3) at the times indicated inC. E, A tsA-201 cell expressing expressing mutant S573A was exposed to cBIMPS (50 μm) during the periods indicated by the solid bar. The absolute value of peak inward Na+ current is plotted as a function of time during the experiment. F, Bar graphs showing the response to 50 μm cBIMPS in tsA-201 cells expressing WT, PKATOT, or S573A mutant Na+ channels.
DISCUSSION
Activation of D1-like dopamine receptors in hippocampal pyramidal neurons reduces Na+ current via phosphorylation of the Na+ channel by PKA
This study describes a complete signaling pathway linking activation of a neurotransmitter receptor on the cell surface of a neuron to PKA-dependent phosphorylation of a specific site on the Na+ channel α subunit for the first time. Earlier work had shown that Na+ currents in cultured rat brain neurons and in mammalian cell lines expressing the α subunit of the rat brain type IIa Na+ channel were reduced by application of the catalytic subunit of PKA to the cytoplasmic surface of excised patches of membrane (Li et al., 1992). That reduction occurred without significantly altering the voltage-dependent properties of the channel or its rate of inactivation. The present work demonstrates that D1-like dopamine receptor activation in acutely isolated hippocampal neurons produces a similar modulation of Na+ current and that the modulation requires activation of PKA. Furthermore, we show that PKA-dependent reduction of Na+ current is abolished in a mutant Na+ channel α subunit in which each of the serines in PKA consensus sites that have been shown to be phosphorylated by PKA in biochemical experiments (Rossie et al., 1987; Rossie and Catterall, 1989; Murphy et al., 1993) has been mutated to alanine (Smith and Goldin, 1996, 1997), as well as in the single serine mutant S573A. Thus, the reduction in Na+ current by activation of D1-like dopamine receptors requires phosphorylation of serine 573. Experiments with other single-site mutants will be required to determine whether the other phosphorylated serine residues may also have significant effects in mammalian cells. In Xenopusoocytes, phosphorylation of serines 610 and 623 has a small effect, whereas phosphorylation of serines 554 and 687 has none (Smith and Goldin, 1997).
There have been few reports of receptor-mediated modulation of Na+ channel activity in neurons. Na+ currents in Aplysia neurons are modulated by the peptide FMRFamide (Brussaard et al., 1991), but the mechanism of receptor coupling to Na+ channel modulation is unknown. Consistent with the present results in hippocampal neurons, activation of dopaminergic receptors modulates whole-cell Na+ current in neostriatal neurons via a PKA-dependent signaling pathway (Surmeier et al., 1992; Schiffmann et al., 1995). Because striatal neurons are specialized for response to dopaminergic neurotransmission, and dopamine has regulatory effects in them that are not observed in other neurons, extension of this regulatory mechanism for Na+ channels to hippocampal neurons suggests that it may be a widely distributed component of the response to dopamine and other neurotransmitters that activate adenylate cyclase.
In hippocampal neurons work from our laboratory has previously shown that activation of muscarinic receptors modulates whole-cell Na+ current (Cantrell et al., 1996a). Those effects occur by a pathway requiring activation of PKC but not activation of PKA. The dopaminergic pathway described here is a distinct pathway for Na+ channel modulation that is distinguished by its dependence on PKA phosphorylation. Thus, two biochemically distinct pathways for Na+ current modulation by neurotransmitters are present in hippocampal neurons.
The effects of PKA activation on Na+ currents demonstrated in the present paper are similar to previous results using cultured rat brain cells or expressed rat brain type IIA Na+ channel α subunits (Li et al., 1992, 1993). A notable exception is that phosphorylation of Ser1506 in the intracellular loop connecting homologous domains III and IV by PKC was required to observe measurable PKA-dependent modulation in a mammalian cell line in the previous study (Li et al., 1993). In the present work, PKA-dependent modulation was not blocked by inclusion of the peptide inhibitor of PKC, PKCI19–36, in the recording pipette. We have found that this difference in the requirement for PKC activity to observe inhibition of Na+ currents by activation of PKA is caused by differences in the holding potential in the two studies (Cantrell et al., 1997). At a holding potential of −110 mV, as used in the studies of Li et al. (1993), little effect of PKA on Na+ current is observed unless PKC is also activated. At more positive holding potentials, the requirement for PKC activation is progressively reduced and is insignificant at the holding potential of −75 mV used here. These results show that the convergent regulation of Na+ channels by the PKA and PKC pathways is voltage-dependent. The voltage dependence of these effects of phosphorylation provides an additional element of specificity and sensitivity to modulation of brain Na+ channels.
Dopaminergic receptor subtypes responsible for modulation
All five of the known dopamine receptor subtypes are expressed in the hippocampus (Meador-Woodruff et al., 1994). The D1-like (D1 and D5) dopamine receptors stimulate adenylate cyclase and increase PKA activity (Stoof and Kebabian, 1981, 1984; Sibley and Monsma, 1992;O’Dowd, 1993). Although we have not yet determined which receptor subtype is responsible for mediating the effects we observe, the D1 and D5 dopamine receptor subtypes are good candidates for mediating the PKA-dependent reduction of Na+ current that we have characterized in hippocampal pyramidal neurons. A more detailed determination, however, must await the development and availability of better subtype-specific blockers.
Functional implications of Na+ channel modulation through D1-like dopamine receptors in the hippocampus
The hippocampus receives a rich dopaminergic innervation from the mesocorticolimbic dopamine system, and multiple dopamine receptor subtypes are expressed on the cell surface of hippocampal pyramidal neurons (Civelli et al., 1993; Meador-Woodruff et al., 1994). The effects of dopamine in the hippocampus are primarily inhibitory, resulting in elevation of spike threshold, slower firing rates, and in some cases membrane hyperpolarization (Biscoe and Straughan, 1966;Herrling, 1981; Stanzione et al., 1984; Pockett, 1985; Malenka and Nicoll, 1986; Berretta et al., 1990). Although it is currently postulated that these effects are primarily a consequence of enhancement of a slow Ca2+-dependent K+ conductance (Bernardo and Prince, 1982; Hass and Konnerth, 1983; Pockett, 1985; Malenka and Nicoll, 1986), it is likely that these effects are mediated by a concerted action of the transmitter on several conductances including the Na+ channel. Reduction of neuronal Na+ currents would be expected to influence the excitability of the target neurons strongly. For example, reduction of peak Na+ current would be expected to shift the voltage threshold for action potential generation toward more depolarized potentials. Thus, a stronger depolarization would be required to elicit a response. The frequency at which the cell is capable of generating action potentials might also be reduced. Additionally, reduction in peak Na+ current by PKA could serve as a neuroprotective safeguard, preventing excitotoxicity resulting from prolonged hyperexcitability. The effects of activation of D1-like receptors are consistent with a role for modulation of Na+ currents as an important contributing factor to the inhibitory effects of dopamine in the hippocampus.
Footnotes
This research was supported by National Institutes of Health Grant NS15751 to W.A.C., a National Institutes of Health postdoctoral fellowship from Training Grant T32 DA07278 and Individual Postdoctoral Fellowship NS10147 to A.R.C., and National Institutes of Health Grant NS26729 and an Established Investigator Award from the American Heart Association to A.L.G.
Correspondence should be addressed to Dr. William A. Catterall, Department of Pharmacology, University of Washington, Box 357280, Seattle, WA 98195-7280.
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