Abstract
Objectives
Biotin is a B vitamin that is critical for the metabolization of fats, carbohydrates, and amino acids. Deficiency in this vitamin can lead to a variety of adverse conditions. For these reasons, it is important to obtain adequate biotin levels from food sources or dietary supplements. Magnesium Biotinate (MgB) is a novel biotin salt that has been shown to be 40 times more soluble than D-Biotin. The following preclinical study was carried out to further explore the absorption and bioavailability of MgB compared to D-Biotin.
Methods
Forty two male Sprague-Dawley rats were divided into the following groups (n = 7 per group): 1) 0.01 mg D-Biotin/kg body weight (BW), 2) 1.0 mg D-Biotin/kg BW, 3) 100 mg D-Biotin/kg BW, 4) 0.01 mg MgB/kg BW, 5) 1.0 mg MgB/kg BW, 6) 100 mg MgB/kg BW. Rat diets included egg whites to standardize absorbable biotin levels. The duration of the study was 35 days. At the end of the study, blood samples were collected and rats were sacrificed to obtain tissue samples.
Results
Results showed that biotin serum levels were 47% higher when rats were given 100 mg of MgB compared to 100 mg of D-Biotin (p < 0.05). Brain biotin levels were 57% and 20% higher when rats were given 1 mg and 100 mg of MgB, respectively, compared to corresponding doses of D-Biotin (p < 0.05) (Figure 1). Liver biotin levels were 27% and 17% higher when rats were given 1 mg and 100 mg of MgB, respectively, compared to corresponding doses of D-Biotin (p < 0.05). Final body weight and feed intake did not differ between groups. Safety blood panel measurements (AST, ALT, creatine, urea, cholesterol, and triglycerides) did not differ between groups. No adverse effects were observed.
Conclusions
Results from this study demonstrate that MgB is a safe, bioavailable form of biotin that has superior absorption to D-Biotin, with superior uptake in tissues at moderate and high doses.
Funding Sources
This study was funded by JDS Therapeutics, LLC.
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