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The Journal of Neuroscience logoLink to The Journal of Neuroscience
. 1991 Apr 1;11(4):918–927. doi: 10.1523/JNEUROSCI.11-04-00918.1991

Cloning and expression of an Aplysia K+ channel and comparison with native Aplysia K+ currents

PJ Pfaffinger 1, Y Furukawa 1, B Zhao 1, D Dugan 1, ER Kandel 1
PMCID: PMC6575383  PMID: 2010814

Abstract

We describe here the cloning of the Aplysia K+ channel AK01a.AK01a codes for a protein of 515 amino acids, shows considerable homology to other cloned potassium channels, and can be classified as a member of the ShakerK+ channel family. Expression of the AK01a channel in Xenopus oocytes produces a rapidly inactivating outward potassium current (IAK01a) resembling the A-type currents of Drosophila Shaker. Gating for this current is shifted to potentials considerably more positive than the traditional A-currents of Aplysia; we have, however, identified a novel transient potassium current (IAdepoI) in a subset of Aplysia neurons that has similar gating and pharmacological properties to IAK01a.


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