a, Representative immunofluorescence images of oligodendrocytes (Olig2), astrocytes (GFAP), and microglia (Iba1) in cortical brain sections of conventional (conv.) and NBC chimeras, with a wild-type, non-chimeric mouse for comparison. The non-neuronal cells are shown in red; DAPI-stained nuclei are in blue. Scale bar, 10 μm. These experiments were repeated on 9 mice (n=3, NBC chimeras; n=3, conventional chimeras; n=3, non-chimeric wild-type mice). b, Quantification Olig2-, GFAP-, or Iba1-positive cells in cortical brain sections of conventional chimeras, NBC chimeras, and wild-type non-chimeric mice (n=3 each). Data represent mean and SEM; ns, not significant, P>0.05 (one-way ANOVA, Tukey’s post hoc correction for multiple comparisons).