Fig. 7.

Omentectomy did not have any long-term effects on the inflammatory response after CLP. CD-1 mice (n=6 per group) were subjected to omentectomy or sham operation. A group of non-treated mice was also used as a control. Mice were then subjected to CLP (16-gauge needle perforation) 28 days after the initial procedures, and lungs were harvested 3 hours post CLP. Cytokine mRNA levels (TNF-α, IL-6, and IL-10) in the lungs were measured by quantitative real-time PCR (qPCR). Results are expressed as means±SEM (n=5–7 in each group), and statistical analysis for the comparison between groups was performed by one-way ANOVA followed by the Tukey’s Multiple Comparison Test.