Table 1. Primers for amplification and sequencing of the mitochondrial genome of Paragonimus ohirai.
| Primer | Sequence (5′->3′) | Location |
|---|---|---|
| URNLFa | AGCCAGGTTGGTTCTTATCTAT | rrnL |
| URNSRa | TACCWTGTTACGACTTAHCWCA | rrnS |
| TRECOBFa | CAGATGTCYTATTGGGCTGC | cob |
| TRECOBRa | GAACHRVCCACARYCCCTTAAAC | cob |
| JB3Fa | TTTTTTGGGCATCCTGAGGTTTAT | cox1 |
| JB4.5Ra | TAAAGAAAGAACATAATGAAAATG | cox1 |
| PO1R | ACATAGCCACTAACACAGCAC | nad2 |
| PO2F | TGGTTCGGGTGTTTTCTGCG | cob |
| PO3R | CCCACTCAAAACGAAACCTAAG | atp6 |
| PO4F | CGGTGAGGGTTCCTCTGTTG | tRNAAla |
| PO5F | GTCTCACAATCCCAAATCCTGG | cox1 |
| PO6F | GTTTGGGAAGTGTTGTCTGGG | cox1 |
| PO7F | TCCTTTTAGGGGAGTAAAGGCC | cob |
| PO8R | GCACAACCGCAACAAAGACC | atp6 |
| PO9R | AGCAGCCCAATCAGAGTTACC | rrnS |
| PO10F | CTGCACCAAATCCGAACGCC | nad3 |
| PO11F | CTTATGGAACTGCAGCACTTC | cox2 |
| PO12R | CGTACCCAACAAACATAAACC | nad5 |
| PO13F | AATTTTTCGTGTTAGAGGGAG | tRNAGlu |
| PO14R | GAAGATAACGAATTAGCCAAAC | cob |
| PO15F | GTGGGTACTTGTATTCTGTG | nad6 |
| PO16R | GTCAAAACACAAAACAAACAC | nad5 |
| PO17R | CGCCGTAATTGAAGAACC | cox3 |
| PO18F | GGTGTAGAAGGGTTGCAC | tRNAGlu |
| PO19F | GGCACTTTTTAAGGCTGGTGG | nad5 |
| PO20R | ACCTCAGATTGGAAGGAAAGCAC | NR |
| PHOR | GTTACCAAAGGATCCGCCTGC | cob |
| PHOWR | CGGTATGTACCCCAACTAAATC | rrnS |
| POHF | ATGATTTGCAGGAGATTTCGGAC | rrnS |
| PHOGLUR | CCTCTAACACGAAAAATTAAC | tRNAGlu |
| GLYFa | AGTATKYYGTCTTTCCAAGTC | tRNAGly |
| GLYRa | ACKAGACCHCYGACTTGGAAAGAC | tRNAGly |
Notes.
a
Platyhelminth-universal primers used for inital amplification of the corresponding genes/regions to get sequences to design further primers.