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. 2019 May 21;4(4):e00202-19. doi: 10.1128/mSystems.00202-19

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Copyright © 2019 Lee et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — Complementation assay for resistant strains performed with KPHS_33600. (A) A meropenem disk diffusion assay showed an inhibitory zone diameter increase (from 10 to 27 mm) resulting from complementation of K56 with KPHS_33600. (B) A meropenem Etest strip assay revealed that the MICs of K56::ZpUC19 and K56::ZpUC19-KPHS_33600 were ≥32 and 0.25 μg/ml, respectively, as indicated by the red arrows. The data were confirmed by CLSI broth dilution tests. Upon complementation, a 128-fold reduction in the MIC for the resistant strain was seen as indicated.