Skip to main content
. 2019 May 31;9(14):4006–4018. doi: 10.7150/thno.32720

Figure 1.

Figure 1

Illustration of lentiviral constructs and characterization of HMGN1-AFP-expressing lentivirus (lenti-HA) in vitro. (A) Illustration of HMGN1-AFP (HA) -expressing constructs. CMVp refers to CMV promoter. Insulin represents Insulin signal peptide. (B) Western blot analysis to show the expression of HA, AFP and HMGN1 in the supernatant and cell lysates. HMGN1 was used as a control. Total protein (20 μg) from supernatants or cell lysates was loaded and GAPDH was used as a loading control. (C) Flow cytrometric analysis of surface markers and co-stimulatory molecules on bone marrow-derived DCs (BMDCs) transduced with lenti-HA, lenti-AFP or lenti-vector (empty vector used as a negative control). (D) Measurement of IL-2 and IFN-γ in supernatants of splenic T lymphocytes primed by lenti-HA, lenti-AFP, lenti-HMGN1 or lenti-vector-transduced DCs with ELISA. The comparison was conducted between lenti-HA and other groups or lenti-AFP and other groups (n=5, **P<0.01). (E) Cytolysis assay for murine hepa1-6 cells with effector T cells at the E: T (Effector: Target) ratio of 10:1. The comparison was conducted between lenti-HA and other groups or lenti-AFP and other groups (n=5, **P<0.01). N.s refers to not significant. Two-tailed t test was used for statistical analysis and all experiments were repeated twice (two repeated experiments yielded similar results and thus one representative result was shown).