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Generation of recombinant MCMV expressing vCXCL-1Tol. (A) Bacterial artificial chromosome (BAC) schematic of vCXCL-1Tol insertion into the mck2 locus. (B) HindIII RFLP analysis of the BACs. The triplet banding patterns are boxed in red. (C) Western blot analysis of the viral supernatant of MCK2-2A-vCXCL-1Tol MCMV. (D) Single-step growth curve (MOI of 5.0). DPI, day postinfection. (E) Multistep growth curves (MOI of 0.05). Values are average titers ± SD (error bars).
