Effects of androgen or estrogen treatment on Crh gene expression within the PVN of ADX’d and GDX’d males. (a) PVN CRH mRNA levels were measured in 2-day ADX’d and GDX’d male mice given TP (1 mg/kg BW, s.c.), DHTP (1 or 10 mg/kg BW, s.c.), or vehicle, daily for 2 days. (b) PVN CRH mRNA levels were measured 2 days following ADX and GDX of male mice who were also administered EB (25 or 250 μg/kg BW, s.c.), 3β-diol P (1 mg/kg BW, s.c.), or vehicle, daily for 2 days. Mean absolute levels of PVN CRH mRNA normalized to the amount of input cDNA ± SEM of n = 5 to 7 animals are shown. *P < 0.05; **P < 0.01.