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. 2017 Feb 27;32(1):80–88. doi: 10.1007/s12250-016-3936-9

A new strain of Crimean-Congo hemorrhagic fever virus isolated from Xinjiang, China

Rong Guo 1, Shu Shen 2, Yanfang Zhang 2, Junming Shi 2, Zhengyuan Su 2, Dan Liu 3, Jinliang Liu 2, Juan Yang 2, Qiguo Wang 1, Zhihong Hu 1, Yujiang Zhang 1,✉, Fei Deng 2
PMCID: PMC6598972  PMID: 28251517

Abstract

Crimean-Congo hemorrhagic fever virus (CCHFV) is a highly pathogenic tick-borne virus with a fatality rate of up to 50% in humans. CCHFV is widely distributed in countries around the world. Outbreaks of CCHFV infection in humans have occurred in prior years in Xinjiang Province, China. Epidemiological surveys have detected CCHFV RNA in ticks and animals; however, few isolates were identified. In this study, we identified and isolated a new CCHFV strain from Hyalomma asiaticum asiaticum ticks collected from north of Tarim Basin in Xinjiang, China. A preliminary investigation of infection and antigens expression of CCHFV was performed in newborn mice. The target tissues for CCHFV replication in newborn mice were identified. The analysis of the phylogenetic relationships with other Chinese strains suggested that diverse genotypes of CCHFV have circulated in Xinjiang for years. These findings provide important insights into our understanding of CCHFV infection and evolution as well as disease prevention and control for local residents. graphic file with name 12250_2016_3936_Fig1_HTML.jpg

Electronic Supplementary Material

Supplementary material is available for this article at 10.1007/s12250-016-3936-9 and is accessible for authorized users.

Keywords: Crimean-Congo hemorrhagic fever virus (CCHFV), newborn mice, genotype, phylogenetic analysis

Electronic supplementary material

12250_2016_3936_MOESM1_ESM.pdf (116.3KB, pdf)

A new strain of Crimean-Congo hemorrhagic fever virus isolated from Xinjiang, China

Acknowledgments

This work was supported by the Science and Technology Basic Work Program (2013FY113500) from the Ministry of Science and Technology of China.

Footnotes

These authors contributed equally to this work.

ORCID: 0000-0001-9436-2595

ORCID: 0000-0002-5385-083X

Compliance with Ethics Guidelines

The authors declare that they have no conflict of interest. Animal experiments in this study were approved by the ethics committees of Wuhan Institute of Virology, Chinese Academy of Sciences (Approval number: WIVH01201 501).

Author Contributions

RG, JMS, JLL, ZYS, and QGW performed the ticks collection in Xinjiang and prepared the homogenates for newborn mice inoculation; RG and YFZ performed the mice inoculation and harvested the tissue samples, and performed the virus passage and IFA on cells; DL conducted the tissue sections from sacrificed mice and pathology analysis of CCHFV; ZYS and JY performed the CCHFV RNA detection in mice tissues. JMS and SS performed sequence of the genome with the phylogenetic analysis. YJZ and FD conceived of the study; RG and SS wrote the manuscript; YJZ and FD checked and finalized the manuscript.

Supplementary Table S1 is available on the websites of Virologica Sinica: www.virosin.org; link.springer.com/journal/12250.

Contributor Information

Yujiang Zhang, Email: xjsyzhang@163.com.

Fei Deng, Email: df@wh.iov.cn.

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Associated Data

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Supplementary Materials

12250_2016_3936_MOESM1_ESM.pdf (116.3KB, pdf)

A new strain of Crimean-Congo hemorrhagic fever virus isolated from Xinjiang, China

Articles from Virologica Sinica are provided here courtesy of Wuhan Institute of Virology, Chinese Academy of Sciences

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