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. 2019 Jul;104(7):e322–e325. doi: 10.3324/haematol.2018.202374

Figure 2.

Figure 2.

Analysis of STAT3, IL10, BCL3, IL2RA, and SOCS3 mRNA expression in Epstein-Barr virus (EBV)-transformed B-cell lines. (A-E) EBV-transformed B cell lines from two healthy donors or the STAT3 gain-of-function (GOF) patient were kept overnight in serum-free medium and then either untreated or stimulated for 1 hour with IL-6 (200 ng/mL) and IL-10 (200 ng/mL) to investigate STAT3, IL10, BCL3, IL2RA, and SOCS3 mRNAs by RT-qPCR. (F) Western blot analysis of STAT1 and STAT2 levels in cytoplasmic extracts from EBV-transformed B cells from a control subject or the STAT3 GOF patient. Cells were cultured overnight without serum and then stimulated with IFNα (1000 U/mL) for 30 minutes. Levels of p-STAT1, p-STAT2, total STAT1 and STAT2 in the cytoplasmic fractions were evaluated. Samples were also immunoblotted for tubulin, a cytoplasmic marker.