Table 1.

Comparing gene targeting efficiency using different nucleases and transfection methods.

Nuclease	Transfection method	Donor plasmids (ng DNA/106 cells)	Transfection efficiency (%)	Fold improvement over “no nuclease” control	Proportion double positive	Reference to figure
CRISPR	Amaxa	300	1.2	6.9x	8.4%	2C
CRISPR	Amaxa	100	0.5	10x	3.3%	2D
TALEN	Amaxa	300	1.1	6.1x	8.3%	2E
TALEN	PEI	300	0.87	1.7x	19%	2F
TALEN	Maxcyte	600	5.1	51x	7.4%	-
TALEN	Maxcyte	200	2.7	89x	3.6%	-