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. 2019 Jun 4;11(5):965–976. doi: 10.1080/19420862.2019.1612690

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© 2019 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 7. — Fed-batch analysis of top producing stable clone derived from GSwt + MSX and GS (R324C) selection. The top producing and stable single clones isolated from GSwt + MSX (a) and R324C (b) stable pools derived from CHO-GS^−/- clone 2 were seeded in glutamine-free 50/50 medium. Cells were fed with EX-CELL Advanced Feed 1 at 7.5% (v/v) at Day 3, 5, 7, 9 and 11. Glucose levels were maintained at above 4 g/L. Viable cell numbers, viability, and antibody titers were assessed at the time points indicated in the graph, and fed-batch cultures were terminated when the viability dropped below 50%.