Skip to main content
. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Endocrinology. 2008 Sep 25;150(2):720–726. doi: 10.1210/en.2008-0941

Figure. 1. Human MRAPα interacts with and promotes trafficking of MC2R to the cell surface producing an ACTH responsive receptor.

Figure. 1

(A) HA-MC2R was detected at the cell-surface of transiently transfected CHO cells by immunofluorescent staining only when co-expressed with MRAP (Bar, 10μm). (B) Cell culture plate based fluorescent cell surface assay showing surface anti-HA staining of live cells as a percentage of total anti-HA staining in permeabilized cells. There was a significant increase in HA-MC2R detected at the cell surface when co-expressed with MRAP-FLAG. Mean ± SEM; n=3; *, P < 0.05. (C) Luciferase assay to measure cAMP response following 6 hour 10-6 M ACTH stimulation. There is a significant increase in cAMP following ACTH stimulation when HA-MC2R is co-expressed with MRAP-FLAG. Mean ± SEM; n=3; **, P < 0.005. (D and E) Co-immunoprecipitation between HA-MC2R and MRAP-FLAG. L = Lysate, IP = Immunoprecipitation. * represents Light Chain. (E) Arrow heads and vertical bar represent MC2R.