Supplemental Fig. 5.

Abundance of significantly affected molecules associated with adhesion and activation of the NF-κB pathway, as found per proteomics, reduced after 1 mM 4-PBA treatment.

Quantitative real-time PCR (qPCR) analysis of RNA isolated from keratinocyte cell lines before and after 1 mM 4-PBA 24 h treatment for molecules associated with cell adhesion and activation of the NF-κB pathway. Expression of PPFIA4, HERC3, LRP1 and AREG normalized to the expression of GAPDH is shown as the percentage of expression in untreated cells. 4-PBA treatment upregulated the expression of all four genes. Values represent mean ± S.E.M., paired t-test was used [PPFIA4: for KRT14mut P = .7074, for KRT5mut *P = .0065; HERC3: for KRT14mut P = .0719, for KRT5mut P = .0657; LRP1: for KRT14mut P = .1811, for KRT5mut P = .1165; AREG: for KRT14mut P = .1007, for KRT5mut P = .3533].