Abstract
Background
Osteosarcoma is one of the most common bone tumors, with strong local aggressiveness and early metastasis. The aim of this study was to describe the epidemiological data and evaluate the prognostic factors for overall survival (OS) and cause-specific survival (CSS) in patients with non-metastatic osteosarcoma.
Material/Methods
Patients histologically diagnosed with non-metastatic osteosarcoma between 2005 and 2014 were selected from the Surveillance, Epidemiology, and End Results (SEER) database. Survival analysis, machine learning, and Lasso regression were used to identify the prognostic factors for OS and CSS, and the accuracy of the nomograms was tested and compared with the American Joint Committee on Cancer (AJCC) staging systems.
Results
The entire cohort comprised 1000 patients with non-metastatic osteosarcoma. The multivariable analysis suggested that age, tumor size, grade, and American Joint Committee on Cancer (AJCC) T staging were independent prognostic factors for OS and CSS. Additionally, the nomograms based on these results could better predict probability of OS (Internal validation C-index, 0.7095) and CSS (0.7100) compared with the sixth (OS: 0.613; CSS: 0.628) and seventh edition AJCC staging systems (0.602, 0.613).
Conclusions
Relatively young age and low histopathological grade were favorable factors for both OS and CSS. Nomograms based on multivariable models worked well in predicting the probability of death for patients with non-metastatic osteosarcoma.
MeSH Keywords: Nomograms, Osteosarcoma, Prognosis, Survival Analysis
Background
Osteosarcoma is one of the most common categories of bone malignancies, with an incidence of 0.2% to 0.5% [1,2]. It most often occurs in teenagers and arises from bone tissue in both extremities and the spine, with the most common site being the metaphysis of long bones. With regard to osteosarcoma of the extremities, approximately 90%–95% of patients can be successfully treated with limb-sparing surgery and three-quarters can be cured with current multimodality treatment [3–6]. However, it is not easy to perform an en bloc tumor resection for spine osteosarcoma, and it exhibits strong local aggressiveness with a high rate of local recurrence. Moreover, osteosarcoma is likely to metastasize early through hematogenous spread (about 15–20%) [3,7,8]. Therefore, osteosarcoma has disastrous effects on individuals and society due to its major cohorts affected, undesirable prognosis, and early metastasis.
To improve the prognosis of non-metastatic osteosarcoma, it may be extremely important to find the truly significant prognostic factors. In previous case series, factors such as age at diagnosis, tumor size and location, pathology, presence and location of metastases, surgical strategy, surgical margin, and histologic respond to chemotherapy have been reported to affect overall survival (OS) [4,8–13]. However, criteria used for evaluation of surgical treatment vary among centers and even different surgeons, which may increase the heterogeneity of samples. Additionally, the relatively small sample size in a single center may lower the accuracy of the constructed nomograms of osteosarcoma.
Therefore, it was necessary to construct more precise prediction models and explore the most significant prognostic factors in patients with non-metastatic osteosarcoma after primary site surgery using big data. In this study, we used the SEER database and combined regression analysis methods (Kaplan-Meier method, Cox proportional hazards regression model, and Lasso regression) and a machine learning model (random forest) to explore the most significant predictors based on a relatively large sample size to identify the prognostic factors and construct nomograms for non-metastatic osteosarcoma.
Material and Methods
Patient selection
The study was approved by the Ethics Committee of the First Affiliated Hospital of Zhengzhou University (No. KEYAN-2018-LW-039). We selected patients from the SEER database, which contains data on cancer occurrences in 18 areas of the United States and covers approximately 26% of the population, and the characteristics of the SEER population are representative for the general US population. The SEER program’s standard for case completeness is 98% and all patients were surveilled for 10 years after routine treatment until death or loss to follow-up [2,14]. Only patients histologically diagnosed with osteosarcoma from 2005 to 2014 were included. Exclusion criteria included tumor size code 000/888/989-999 (unknown or inaccurate) and extent codes 00 (in situ) or 99 (unknown extent). We also excluded patients who were not diagnosed with osteosarcoma by biopsy, who were at N0 or M0 stage, and who did not undergo primary site surgery. Patients with non-primary osteosarcoma or with missing data (survival months, race, marital status, grade, and radiotherapy data) were excluded.
Data extraction
Variables in the study were obtained from SEER database on March 6th, 2018, including baseline demographics of patients (age at diagnosis, sex, race), characteristics of tumor (tumor size, tumor extent, primary site, histologic subtype, tumor node metastasis (TNM) state), treatment (radiotherapy, chemotherapy), and socioeconomic status (marital status, education background, family income, and employment status). As researching endpoints, we retrieved osteosarcoma cause-specific survival (CSS) and overall survival (OS) from the database.
Statistical analysis
As an initial descriptive statistic, dichotomous variables were reported as percentages, while continuous variables were reported as mean and median (range). To find the most significant predictors, we used 3 statistical methods. First, the chi-square test was used to compare outcome between the elements of each categorical variable. Continuous variables in normal distribution and homogeneity of variance were compared by using the 2-sample t test, otherwise, the Mann-Whitney U test was performed. In addition, the Kaplan-Meier method was applied to obtain OS and CSS between each type contained in a categorical variable. For Kaplan-Meier analysis, we divided continuous variables into new classifications following the National Comprehensive Cancer Network guidelines [15,16] (age: <15 years, 15–39 years, >39 years; tumor size: ≤80 mm, >80 mm). Histopathologically, osteosarcoma were divided into 12 subtypes according to the ICD-0-3 coding system: osteosarcoma, NOS (no other specific) (9180/3), chondroblastic osteosarcoma (9181/3), fibroblastic osteosarcoma (9182/3), telangiectatic osteosarcoma (9183/3), osteosarcoma in Paget disease (9184/3), small cell osteosarcoma (9185/3), central osteosarcoma (9186/3), intraosseous well-differentiated osteosarcoma (9187/3), parosteal osteosarcoma (9192/3), periosteal osteosarcoma (9193/3), high-grade surface osteosarcoma (9194/3), and intracortical osteosarcoma (9195/3), which contains both histological information and location [17]. Therefore, a subgroup Kaplan-Meier analysis based on histology and location information was also performed (histology group: osteosarcoma, NOS, chondroblastic osteosarcoma, fibroblastic osteosarcoma, high-grade surface osteosarcoma, and others (small cell osteosarcoma, telangiectatic osteosarcoma, intraosseous well-differentiated osteosarcoma) (location: parosteal osteosarcoma, periosteal osteosarcoma and central osteosarcoma). The log-rank test was applied to compare the survival curves of each type of variable. For further analysis, the random forest (Ntree=500) was constructed for all variables. Random forest is an ensemble of unpruned decision trees, induced from bootstrap samples of the data, using random feature selection in the tree induction process. The mean decrease Gini (MDG) involved in the random forest algorithm was used to rank the influencing factors with probability of death. MDG provided ways to quantify which index contributed most to classification accuracy. Greater MDG indicated the degree of impurity arising from a category could be reduced farthest by 1 variable, thus suggesting an important associated index. Out-of-bag (OOB) error is the parameter for evaluating the classification accuracy of random forest [18].
After these procedures, we selected the best subsets of significant predictors to conduct the Cox proportional hazards model. Likelihood ratio test, Ward test, and log-rank test were used for model diagnosis. Eventually, we developed a model consisting of optimum predictors. Then, Lasso regression was performed to ensure that the multifactor models were not overfitting. The nomograms based on Cox proportional hazards model were built to predict the probability of OS and CSS. The discrimination and calibration of predictors were accessed by the C-index of internal validation and calibration curve, respectively.
Only 2-sided P value <0.05 was considered as statistical significance. All statistical analyses were conducted with R version 3.3.1 software (Institute for Statistics and Mathematics, Vienna, Austria; www.r-project.org). The R packages survival, survminer, ggplot2, pwr, and randomForest were used for modeling (including Power of Hypothesis Tests) and drawing survival curves. The nomograms were drawn by the rms package.
Results
Patient characteristics
The process of data selection is shown by the flow chart in Figure 1. The cohort consisted of 1000 patients with non-metastatic osteosarcoma from the SEER database. The characteristics of all the patients are described in Supplementary Table 1. The patients included 470 females and 530 males, with a mean age of 25.3 years (median 18.0 years, range, 3.0 to 89.0 years), similar to previous studies [10,22]. These non-metastatic osteosarcomas were dominantly localized or regional (96.5%), grade IV (56.6%), and NOS histologically (59.9%), with a median size of 85.0 (range, 5.0 to 486.0) mm. During 10 years of follow-up, the median survival time was 46.8 (range, 0 to 119) months. The mean follow-up time was 46.77±37.90 months and all patients were active at follow-up. With the respect to the endpoint, 203 (20.3%) and 187 (18.7%) patients died of all and specific causes, respectively. Among all patients, most were unmarried (79.1%), while education levels and family incomes were distributed evenly.
Figure 1.
Flow diagram of patient selection.
Univariate analysis and random forest
The OS and CSS Kaplan-Meier curves of age and grade are shown in Supplementary Figure 1. The survival curves between age groups showed that OS and CSS were longest in patients under 15 years old and shortest in patients over 40 years old (P<0.001, Supplementary Figure 1A, P<0.001, Supplementary Figure 1B). Additionally, patients with grade I and grade II tumors had better OS (P<0.001, Supplementary Figure 1C) and CSS (P<0.001, Supplementary Figure 1D) compared with patients with grade III and grade IV tumors. In addition, a subgroup Kaplan-Meier analysis based on histology and location information was also performed, showing no significant difference in prognosis compared with the reference group (osteosarcoma, NOS) in each subgroup (Supplementary Figure 2A–2D).
Univariate analysis and random forest for OS (OOB=20.60%) and CSS (OOB=19.50%) are shown in Table 1. All tumor characteristics, except for tumor size classified by 8 cm, showed significant associations with the survival time of patients in both parametric and non-parametric tests and in Kaplan-Meier survival analysis. In addition, they ranked in the top 7 MDG of the random forest model, the same as the age of patients. However, as a continuous variable, tumor size was significant in the parametric and non-parametric tests, ranking first in the random forest model. Multiple evidence in the guidelines showed that tumor size was associated with prognosis [19], which was also in line with our clinical experience. Therefore, we included these 6 variables (age, primary site, grade, histological subtype, AJCC T staging, and tumor size) in our further Cox modeling.
Table 1.
Results of single-factor analysis and random forest model.
Variables | Overall survival (OS) | Cancer-specific survival (CSS) | ||||
---|---|---|---|---|---|---|
P value of parameter or non-parametric test | P value of Kaplan-Meier survival analysis | MDG | P value of parameter or non-parametric test | P value of Kaplan-Meier survival analysis | MDG | |
Categorical age | <0.001* | <0.001* | 17.9285255 | <0.001* | <0.001* | 16.1492625 |
Race | 0.812 | 0.618 | 14.6161796 | 0.932 | 0.695 | 13.4284526 |
Sex | 0.185 | 0.370 | 11.213705 | 0.093 | 0.215 | 10.166965 |
Marital status | <0.001* | 0.046 | 8.571557 | <0.001* | <0.001* | 7.672955 |
Primary site | <0.001* | <0.001* | 18.1616484 | <0.001* | <0.001* | 17.2527824 |
Grade | <0.001* | <0.001* | 20.0879203 | 0.001* | 0.001* | 18.4483283 |
Histological subtype | 0.002* | 0.005* | 26.2889922 | 0.004* | 0.006* | 23.6393802 |
AJCC.T staging | 0.009* | 0.001* | 12.1364207 | 0.003* | <0.001* | 11.6476987 |
Tumor size, mm | 0.020* | 0.110 | 66.4103101 | 0.008* | 0.068 | 64.3342381 |
Radiation | 0.002* | 0.001* | 6.437203 | 0.009* | 0.003* | 6.173988 |
Chemotherapy | 0.116 | 0.124 | 6.346112 | 0.134 | 0.138 | 5.906247 |
9th grade education | 0.100 | 0.152 | 9.34257 | 0.097 | 0.146 | 8.754056 |
High school education | 0.791 | 0.848 | 7.818148 | 0.716 | 0.772 | 7.402536 |
At least bachelor’s degree | 0.735 | 0.619 | 7.475351 | 0.809 | 0.693 | 6.842956 |
Median family income | 0.562 | 0.447 | 7.929627 | 0.599 | 0.473 | 7.066662 |
Unemployed | 0.258 | 0.675 | 8.559674 | 0.307 | 0.728 | 7.783507 |
Families below poverty level | 0.721 | 0.669 | 6.779123 | 0.991 | 0.634 | 6.658563 |
Families above poverty level | 0.967 | 0.991 | 7.147897 | 0.875 | 0.900 | 7.001368 |
Categorical variables were compared by using the Pearson chi-square test. Continuous variables in normal distribution and homogeneity of variance were compared by using the two-sample t test, otherwise, the Mann-Whitney U test was performed. OS – overall survival; CSS – cause-specific survival; MDG – Mean Decrease Gini; AJCC – American Joint Committee on Cancer.
P<0.05.
Because of the controversy over age in previous studies, we also performed univariate analysis, which suggested that older patients (>39 years) tended to have higher pathological grades (P<0.001) and higher AJCC T-stages (P=0.009) (Supplementary Tables 2, 3).
Cox proportional hazards model and Lasso regression
The Cox proportional hazard regression model was constructed to confirm the effects of the covariates mentioned above on the OS and CSS of patients (Table 2). All variables eventually incorporated into the multivariate models were shown to be essential for modeling in the Lasso regression (Figure 2). Compared with patients younger than 15 years old, older age was associated with poorer OS (15–39 years: HR, 1.616; 95% CI, 1.115 to 2.341; P=0.043) (older than 39 years: HR, 3.063; 95% CI, 2.020 to 4.644; P<0.001) and CSS (15–39 years: HR, 1.474; 95% CI, 1.012 to 2.149; P=0.043) (older than 39 years: HR, 2.845; 95% CI, 1.860 to 4.351; P<0.001). Pelvic osteosarcoma, showing a significantly worse prognosis in the survival curve, was not a prognostic indicator in our multivariate model. Furthermore, grade III and grade IV were independently associated with worse OS and CSS (OS with grade III vs. grade I: HR, 4.181; 95% CI, 1.461 to 11.968; P=0.008; CSS with grade III vs. grade I: HR, 4.702; 95% CI, 1.416 to 15.614; P=0.011; OS with grade IV vs. grade I: HR, 3.792; 95% CI, 1.347 to 10.671; P=0.012; CSS with grade IV vs. grade I: HR, 4.394; 95% CI, 1.345 to 14.353; P=0.014). In histological subtypes, only central (HR, 0.312; 95% CI, 0.099 to 0.979; P=0.046) and other subtypes of osteosarcoma (HR, 0.322; 95% CI, 0.118 to 0.881; P=0.027) trended to better OS than osteosarcoma, NOS. As for CSS, other subtypes osteosarcoma (HR, 0.341; 95% CI, 0.125 to 0.935; P=0.037) trended to better outcome than reference. Finally, increasing tumor size was associated with worse OS (HR, 1.005; 95% CI, 1.002 to 1.008; P=0.002) and CSS (HR, 1.006; 95% CI, 1.002 to 1.009; P<0.001), while the results only showed that AJCC T3 staging was a risk factor for poor prognosis of OS (HR, 2.955; 95% CI, 1.383 to 6.313; P=0.005) and CSS (HR, 3.103; 95% CI, 1.441 to 6.682; P=0.004) compared with T1 staging, but not T2 staging, which was distinguished with T1 by 8 cm of tumor size.
Table 2.
Cox proportional hazards regression model for overall survival and cancer – specific survival in patients with non-metastatic osteosarcoma.
Variable | Overall survival (OS) | P | Cancer specific survival (CCS) | P |
---|---|---|---|---|
Hazard ratio (95% CI) | Hazard ratio (95% CI) | |||
Categorical age | ||||
<15 | 1.00 (reference) | 1.00 (reference) | ||
15–39 | 1.616 (1.115 to 2.341) | 0.043* | 1.474 (1.012 to 2.149) | 0.043* |
>39 | 3.063 (2.020 to 4.644) | <0.001* | 2.845 (1.860 to 4.351) | <0.001* |
Primary site | ||||
Bones of skull and face | 1.00 (reference) | 1.00 (reference) | ||
Bones of upper or lower limb | 0.585 (0.359 to 0.953) | 0.031* | 0.629 (0.373 to 1.061) | 0.082 |
Pelvic bones, sacrum, coccyx | 1.486 (0.785 to 2.813) | 0.224 | 1.759 (0.907 to 3.412) | 0.095 |
Rib, sternum, clavicle | 0.822 (0.313 to 2.157) | 0.690 | 0.979 (0.367 to 2.607) | 0.966 |
Vertebral column | 0.842 (0.198 to 3.581) | 0.816 | 1.018 (0.237 to 4.373) | 0.981 |
Grade | ||||
Grade I | 1.00 (reference) | 1.00 (reference) | ||
Grade II | 1.192 (0.345 to 4.118) | 0.782 | 1.606 (0.410 to 6.292) | 0.496 |
Grade III | 4.181 (1.461 to 11.968) | 0.008* | 4.702 (1.416 to 15.614) | 0.011* |
Grade IV | 3.792 (1.347 to 10.671) | 0.012* | 4.394 (1.345 to 14.353) | 0.014* |
Histological subtype | ||||
Osteosarcoma, NOS | 1.00 (reference) | 1.00 (reference) | ||
Central osteosarcoma | 0.312 (0.099 to 0.979) | 0.046* | 0.337 (0.107 to 1.059) | 0.063 |
Chondroblastic osteosarcoma | 0.772 (0.517 to 1.153) | 0.206 | 0.771 (0.509 to 1.168) | 0.219 |
Fibroblastic osteosarcoma | 0.773 (0.433 to 1.382) | 0.385 | 0.631 (0.328 to 1.214) | 0.168 |
High-grade surface osteosarcoma | 0.832 (0.204 to 3.394) | 0.797 | 0.906 (0.222 to 3.703) | 0.891 |
Parosteal osteosarcoma | 0.613 (0.234 to 1.602) | 0.318 | 0.514 (0.177 to 1.493) | 0.221 |
Periosteal osteosarcoma | 0.316 (0.078 to 1.283) | 0.107 | 0.334 (0.082 to 1.358) | 0.125 |
Others | 0.322 (0.118 to 0.881) | 0.027* | 0.341 (0.125 to 0.935) | 0.037* |
AJCC T | ||||
T1 | 1.00 (reference) | 1.00 (reference) | ||
T2 | 0.913 (0.605 to 1.378) | 0.666 | 0.908 (0.593 to 1.391) | 0.657 |
T3 | 2.955 (1.383 to 6.313) | 0.005* | 3.103 (1.441 to 6.682) | 0.004* |
Tumor size | 1.005 (1.002 to 1.008) | 0.002* | 1.006 (1.002 to 1.009) | <0.001* |
OS – overall survival; CSS – cause-specific survival; MDG – Mean Decrease Gini; AJCC – American Joint Committee on Cancer.
P<0.05.
Figure 2.
The Lasso regression variable-filtering process. To avoid overfitting, Lasso regression suggested including 9 and 12 variables when overall survival (OS) (A, B) and cause-specific survival (CSS) (C, D) was the endpoint, respectively.
For sensitivity analysis, we used ANOVA to compare the models reduced by each variable and the full model including all 6 variables, showing each variable was indispensable for modeling: all 6 ANOVAs showed significant statistical results (P<0.05), indicating that after removing any of the 6 variables, the multivariable model was statistically significantly different from the previous model. Therefore, each variable was essential to the modeling process. The statistical power tests also showed the sufficiency of the sample size.
Nomogram
The nomograms predicting the probability 3- and 5-year OS (Internal validation C-index, 0.7095) and CSS (Internal validation C-index, 0.7100) (Figure 3A, 3C) were constructed based on the Cox proportional hazard regression models with the total cohort as the training dataset (available in Supplementary Materials B). The calibration plot of the CIF is shown in supplementary materials Figure 3B and 3D. Even without external validation, the points slightly further from the 45-degree line indicate some inconsistencies between predictions and observations. Additionally, compared with sixth (OS: 0.613; CSS: 0.628) and seventh edition AJCC staging systems (0.602; 0.613), the nomograms predicted the probability of OS (0.7095) and CSS (0.7100) more accurately (Supplementary Figure 3).
Figure 3.
Nomograms and calibration curves of overall survival (OS) (A, B) and cause-specific survival (CSS) (C, D). OS – overall survival; CSS – cause-specific survival; AJCC – American Joint Committee on Cancer; ICD – International Classification of Diseases; O, NOS – osteosarcoma, No other specific; C – central osteosarcoma; Cb – chondroblastic osteosarcoma; Fb – fibroblastic osteosarcoma; Hs – high grade surface osteosarcoma; Iw – intraosseous well differentiated osteosarcoma; Pa – parosteal osteosarcoma; Pe – periosteal osteosarcoma; Sc – small cell osteosarcoma; T – telangiectatic osteosarcoma; Sf – bones of skull and face; V – vertebral column; Rsc – rib, sternum, clavicle; L – bones of upper or lower limb; Psc – pelvic bones, sacrum, coccyx.
Discussion
Osteosarcoma is the most common histologic type of bone tumor, with low incidence rate but high fatality rate [14,20]. As it occurs mainly in adolescents or young adults, osteosarcoma severely harms the social workforce. Prone to early-stage metastasis, many osteosarcoma cases are diagnosed at the time when poor prognosis is inevitable, affecting patients both physically and mentally [9,21]. Thus, finding the most important prognostic factors at the pre-metastasis stage enables timely management and improves prognosis to a great extent, which can be much more effective and less costly.
In our series, we divided age into childhood (<15 years), adolescent and young adult (AYA, 15–39 years), and old adult (>40 years) according to the NCCN Guidelines for Adolescent and Young Adult Oncology [16]. We confirmed that patients older than 40 years had the poorest CSS and OS, not only in univariate and multivariate analysis, but also in the random forest model. Therefore, age was regarded as an independent prognostic factor for postoperative CSS and OS of non-metastatic osteosarcoma in this study. Age at diagnosis, a controversial factor, was not considered by Harting et al. [9] to be a significant independent prognostic variable for OS and disease-free survival in extremities and torso osteosarcoma. However, Grimer et al. [23] and Kager et al. [24] did not draw the same conclusion. It is widely accepted that osteosarcoma in childhood often tends to be highly malignant, leading to poor prognosis [16]. However, in this study, we performed univariate analysis between age and other factors, showing that older patients (>39 years) tended to have higher pathological grades, higher AJCC T-stages, and larger tumor sizes. This is a new fact based on the SEER database and it can explain why increasing age was a risk factor for poor prognosis of OS and CSS.
In many previous studies, tumor size and primary site had been demonstrated to be the most significant prognostic variables for many malignant bone tumors, especially for non-metastatic osteosarcoma [9,25,26], because both of them determine the difficulty of the operation and feasibility of en bloc tumor resection [27]. For instance, either a huge osteosarcoma or a spinal one can invade and involve fateful vessels or the spinal cord, which may compromise sufficient surgical excision range. For malignant tumors, sufficient surgical excision range is the prerequisite for prolonging survival [28,29]. However, our findings suggest that the primary site and tumor size were not independent risk indicators for poor prognosis. In this study, we selected patients with non-metastatic osteosarcoma after primary site operations; therefore, all the candidates were regarded as having sufficient surgical excision range. Consequently, the primary site and tumor size did not affect the OS and CSS in this study.
The subtypes of histologic grade are defined as well differentiated (Grade I), moderately differentiated (Grade II), poorly differentiated (Grade III), and undifferentiated (Grade IV) in the SEER database, while AJCC T staging is widely used as a tumor staging method, setting 8 cm as an important cut-off value to distinguish different T stages [28,29]. Both tumor grade and AJCC T staging were suggested to be independent prognostic factors for postoperative CSS and OS in this study. Compared with AJCC T1 staging, T3 staging (but not T2 staging) was a risk factor for poor prognosis of OS and CSS.
Histopathologically, we divided osteosarcoma into 2 subgroups according to histology and position relation between tumor and bone. In line with some previous research results, in multifactor modeling, most histological subtypes were not significantly associated with patient prognosis compared with the reference group (Osteosarcoma, NOS) in our study [11,22,25,29]. Subgroup analysis obtained similar results. This might be because histological types, such as chondroblastic and fibroblastic, are only a description of the cell components of the tumor, and location only shows the positional relationship between tumor and bone, both of which had little to do with the malignancy of the tumor. However, patients with poorly differentiated and undifferentiated pathological grading showed worse prognosis in our series. Hence, for stratifying tumor pathological grading, our findings suggested that grade and AJCC T staging were better prognostic risk factors than was histological subtype.
Primary site treatment combined with preoperative and postoperative adjuvant therapy is the standard treatment strategy for osteosarcoma, aim at resecting the primary tumor completely, relieving symptoms, reconstructing function, and preventing local recurrence [16,19]. Aggressive surgery associated with systemic chemotherapy (neoadjuvant chemotherapy and adjuvant chemotherapy) and radiotherapy are essential for cure and for controlling localized and micro-metastatic disease, which was shown to be vital to postoperative prognosis in previous research [6,30]. However, our results suggested that the addition of chemotherapy or radiotherapy contributed little to the classification of the random forest model, showing that the variables had little effect on the outcome, in accordance with some previous studies [10,11]. Although patients without metastasis who received definitive surgery were regarded as good candidates for chemotherapy, the addition of chemotherapy did not have any effect on the outcome [10,11]. However, the missing data of the 2 variables in the SEER database must also be considered.
To the best of our knowledge, this is the first study combining conventional univariate and multivariate survival analysis with a machine learning model to explore the most important prognostic factors of osteosarcoma. Nevertheless, our study has some limitations. First, although the study had large sample size and multiple variables based on the SEER database, there were still some inaccurate variables in this database. Second, it has all the limitations inherent in retrospective studies. Third, although the nomogram was verified to be applicable in terms of internal validation, calibration, and clinical usefulness, it included fewer variables than other studies [31]. Last but not least, because the database contained data from multiple centers, its inter-group heterogeneity was not processed, even though we used strict inclusion and exclusion criteria to minimize this heterogeneity. A more rigorous nomogram is required to compensate for the imperfection in survival prediction of this serious disease [32]. In this regard, the nomogram should contain the expression level of biomarkers (such as some newfound prognosis-related functional genetic single-nucleotide polymorphisms (SNPs) and transcription factors) most associated with these prognostic factors, which might be found by weighted correlation network analysis (WGCNA) and deep learning [33–35]. This will be our next research focus.
Conclusions
Despite its limitations, this study shows that increasing age, high histopathological grade, and T staging were the most significant prognostic factors of both OS and CSS in patients with non-metastatic osteosarcoma after primary site operations. In addition, for a tumor with such high mortality, a more accurate prediction model achieving more accuracy and higher safety should be developed, which might be constructed by adding the expression level of some hub-genes in the nomogram.
Acknowledgements
We thank the Surveillance, Epidemiology, and End Results Program team of the National Cancer Institute for allowing use of their data.
Appendix
Supplementary materials A
Supplementary Table 1.
Baseline characteristics of patients with non-metastatic osteosarcoma.
Demographic or characteristic | Total patients (N=1000) | Alive cohort (N=797) | Dead cohort (N=203) | |||
---|---|---|---|---|---|---|
No. | % | No. | % | No. | % | |
Age, years | ||||||
Mean | 25.3 | 23.8 | 31.5 | |||
Median (range) | 18.0 (3.0–89.0) | 17.0 (3.0–84.0) | 22.0 (3.0–89.0) | |||
Categorical age | ||||||
<15 | 316 | 31.6% | 275 | 34.5% | 41 | 20.2% |
15–39 | 483 | 48.3% | 385 | 48.3% | 98 | 48.3% |
>39 | 201 | 20.1% | 137 | 17.2% | 64 | 31.5% |
Sex | ||||||
Female | 470 | 47.0% | 383 | 48.1% | 87 | 42.9% |
Male | 530 | 53.0% | 414 | 51.9% | 116 | 57.1% |
Race | ||||||
Black | 150 | 15.0% | 117 | 14.7% | 33 | 16.3% |
Other | 99 | 9.9% | 78 | 9.8% | 21 | 10.3% |
White | 751 | 75.1% | 602 | 75.5% | 149 | 73.4% |
Tumor size, mm | ||||||
Mean | 96.0 | 93.4 | 106.2 | |||
Median (range) | 85.0 (5.0–486.0) | 85.0 (5.0–369.0) | 95.0 (14.0–486.0) | |||
Categorical tumor size | ||||||
≤80 mm | 452 | 45.2% | 369 | 46.3% | 83 | 40.9% |
>80 mm | 548 | 54.8% | 428 | 53.7% | 120 | 59.1% |
Primary site | ||||||
Bones of skull and face | 105 | 10.5% | 79 | 9.9% | 26 | 12.8% |
Bones of upper or lower limb | 823 | 82.3% | 674 | 84.6% | 149 | 73.4% |
Pelvic bones, sacrum, coccyx | 43 | 4.3% | 22 | 2.8% | 21 | 10.3% |
Rib, sternum, clavicle | 20 | 2.0% | 15 | 1.9% | 5 | 2.5% |
Vertebral column | 9 | .9% | 7 | .9% | 2 | 1.0% |
Grade | ||||||
Grade I | 58 | 5.8% | 54 | 6.8% | 4 | 2.0% |
Grade II | 92 | 9.2% | 85 | 10.7% | 7 | 3.4% |
Grade III | 284 | 28.4% | 216 | 27.1% | 68 | 33.5% |
Grade IV | 566 | 56.6% | 442 | 55.5% | 124 | 61.1% |
Histological subtype | ||||||
Central osteosarcoma | 45 | 4.5% | 42 | 5.3% | 3 | 1.5% |
Chondroblastic osteosarcoma | 151 | 15.1% | 118 | 14.8% | 33 | 16.3% |
Fibroblastic osteosarcoma | 59 | 5.9% | 46 | 5.8% | 13 | 6.4% |
High-grade surface osteosarcoma | 9 | .9% | 7 | .9% | 2 | 1.0% |
Osteosarcoma, NOS | 599 | 59.9% | 458 | 57.5% | 141 | 69.5% |
Parosteal osteosarcoma | 67 | 6.7% | 62 | 7.8% | 5 | 2.5% |
Periosteal osteosarcoma | 19 | 1.9% | 17 | 2.1% | 2 | 1.0% |
Others | 51 | 5.1% | 47 | 5.9% | 4 | 2.0% |
AJCC T staging | ||||||
T1 | 445 | 44.5% | 367 | 46.0% | 78 | 38.4% |
T2 | 534 | 53.4% | 418 | 52.4% | 116 | 57.1% |
T3 | 21 | 2.1% | 12 | 1.5% | 9 | 4.4% |
Radiation | ||||||
Beam radiation | 51 | 5.1% | 31 | 3.9% | 20 | 9.9% |
Combination of beam with implants or isotopes | 2 | 0.2% | 2 | 0.3% | 0 | 0.0% |
None/Unknown | 947 | 94.7% | 764 | 95.9% | 183 | 90.1% |
Chemotherapy | ||||||
No/Unknown | 170 | 17.0% | 143 | 17.9% | 27 | 13.3% |
Yes | 830 | 83.0% | 654 | 82.1% | 176 | 86.7% |
Survival time | ||||||
Mean | 46.8 | 51.1 | 29.7 | |||
Median (range) | 40.0 (0–119.0) | 41.0 (0–119.0) | 25.0 (3.0–89.0) | |||
Marital status | ||||||
Married | 209 | 20.9% | 145 | 18.2% | 64 | 31.5% |
Single/separated/divorced/widowed | 791 | 79.1% | 652 | 81.8% | 139 | 68.5% |
9th grade education | ||||||
Lower 50% | 480 | 48.0% | 393 | 49.3% | 87 | 42.9% |
Upper 50% | 520 | 52.0% | 404 | 50.7% | 116 | 57.1% |
High school education | ||||||
Lower 50% | 496 | 49.6% | 397 | 49.8% | 99 | 48.8% |
Upper 50% | 504 | 50.4% | 400 | 50.2% | 104 | 51.2% |
At least bachelor’s degree | ||||||
Lower 50% | 423 | 42.3% | 335 | 42.0% | 88 | 43.3% |
Upper 50% | 577 | 57.7% | 462 | 58.0% | 115 | 56.7% |
Median family income (in tens) | ||||||
Lower 50% | 496 | 49.6% | 399 | 50.1% | 97 | 47.8% |
Upper 50% | 504 | 50.4% | 398 | 49.9% | 106 | 52.2% |
Unemployed | ||||||
Lower 50% | 459 | 45.9% | 373 | 46.8% | 86 | 42.4% |
Upper 50% | 541 | 54.1% | 424 | 53.2% | 117 | 57.6% |
Families below poverty level | ||||||
Lower 50% | 489 | 48.9% | 392 | 49.2% | 97 | 47.8% |
Upper 50% | 511 | 51.1% | 405 | 50.8% | 106 | 52.2% |
Families above poverty level | ||||||
Lower 50% | 420 | 42.0% | 335 | 42.0% | 85 | 41.9% |
Upper 50% | 580 | 58.0% | 462 | 58.0% | 118 | 58.1% |
OS – overall survival; CSS – cause-specific survival; AJCC – American Joint Committee on Cancer.
Supplementary Table 2.
Univariate analysis results between age and histopathological grade.
Grade | Age | OR | 95% CI | P value |
---|---|---|---|---|
Grade II | 0–14 | 1.00 (reference) | ||
15–39 | 1.167 | 0.426–3.197 | 0.764 | |
>39 | 0.939 | 0.329–2.680 | 0.907 | |
Grade III | 0–14 | 1.00 (reference) | ||
15–39 | 0.415 | 0.181–0.950 | 0.037* | |
>39 | 0.219 | 0.091–0.525 | 0.001* | |
Grade IV | 0–14 | 1.00 (reference) | ||
15–39 | 0.380 | 0.170–0.851 | 0.019* | |
>39 | 0.167 | 0.072–0.388 | <0.001* |
Grade I is the reference group. OR – odds ratio; CI – confidence interval.
P<0.05.
Kaplan-Meier curves of overall survival (OS) (left) and cause-specific survival (CSS) (right) for age (A, B) and grade (C, D).
Kaplan-Meier curves of overall survival (OS) (left) and cause-specific survival (CSS) (right) for histological subtype (A, B) and location subtype (C, D). ICD – International Classification of Diseases; O, NOS – osteosarcoma, No other specific; C – central osteosarcoma; Cb – chondroblastic osteosarcoma; Fb – fibroblastic osteosarcoma; Hs – high-grade surface osteosarcoma; Iw – intraosseous well-differentiated osteosarcoma; Pa – parosteal osteosarcoma; Pe – periosteal osteosarcoma; Sc – small cell osteosarcoma; T – telangiectatic osteosarcoma; Other – small cell osteosarcoma, telangiectatic osteosarcoma and intraosseous well-differentiated osteosarcoma.
Kaplan-Meier curves of overall survival (OS) (left) and cause-specific survival (CSS) (right) for sixth (A, B) and seventh (C, D) edition American Joint Committee on Cancer (AJCC) staging systems.
Supplementary materials B
Supplementary Table 3.
Univariate analysis results between age and American Joint Committee on Cancer T staging.
AJCC T | Age | OR | 95% CI | P value |
---|---|---|---|---|
T2 | 0–14 | 1.00 (reference) | ||
15–39 | 0.984 | 0.736–1.316 | 0.913 | |
>39 | 0.651 | 0.454–0.933 | 0.019* | |
T3 | 0–14 | 1.00 (reference) | ||
15–39 | 0.316 | 0.116–0.864 | 0.025* | |
>39 | 0.306 | 0.084–1.113 | 0.072 |
T1 is the reference group. OR – odds ratio; CI – confidence interval; AJCC – American Joint Committee on Cancer.
P<0.05.
Footnotes
Source of support: This study was supported in part by the National Natural Science Foundation of China (Grant No. 81702659; 81772856; 81501203); the Youth Fund of Shanghai Municipal Health Planning Commission (No.2017YQ054); and the Henan Medical Science and Technology Research Project (Grant No. 201602031)
Conflicts of interest
None.
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Associated Data
This section collects any data citations, data availability statements, or supplementary materials included in this article.
Supplementary Materials
Supplementary Table 1.
Baseline characteristics of patients with non-metastatic osteosarcoma.
Demographic or characteristic | Total patients (N=1000) | Alive cohort (N=797) | Dead cohort (N=203) | |||
---|---|---|---|---|---|---|
No. | % | No. | % | No. | % | |
Age, years | ||||||
Mean | 25.3 | 23.8 | 31.5 | |||
Median (range) | 18.0 (3.0–89.0) | 17.0 (3.0–84.0) | 22.0 (3.0–89.0) | |||
Categorical age | ||||||
<15 | 316 | 31.6% | 275 | 34.5% | 41 | 20.2% |
15–39 | 483 | 48.3% | 385 | 48.3% | 98 | 48.3% |
>39 | 201 | 20.1% | 137 | 17.2% | 64 | 31.5% |
Sex | ||||||
Female | 470 | 47.0% | 383 | 48.1% | 87 | 42.9% |
Male | 530 | 53.0% | 414 | 51.9% | 116 | 57.1% |
Race | ||||||
Black | 150 | 15.0% | 117 | 14.7% | 33 | 16.3% |
Other | 99 | 9.9% | 78 | 9.8% | 21 | 10.3% |
White | 751 | 75.1% | 602 | 75.5% | 149 | 73.4% |
Tumor size, mm | ||||||
Mean | 96.0 | 93.4 | 106.2 | |||
Median (range) | 85.0 (5.0–486.0) | 85.0 (5.0–369.0) | 95.0 (14.0–486.0) | |||
Categorical tumor size | ||||||
≤80 mm | 452 | 45.2% | 369 | 46.3% | 83 | 40.9% |
>80 mm | 548 | 54.8% | 428 | 53.7% | 120 | 59.1% |
Primary site | ||||||
Bones of skull and face | 105 | 10.5% | 79 | 9.9% | 26 | 12.8% |
Bones of upper or lower limb | 823 | 82.3% | 674 | 84.6% | 149 | 73.4% |
Pelvic bones, sacrum, coccyx | 43 | 4.3% | 22 | 2.8% | 21 | 10.3% |
Rib, sternum, clavicle | 20 | 2.0% | 15 | 1.9% | 5 | 2.5% |
Vertebral column | 9 | .9% | 7 | .9% | 2 | 1.0% |
Grade | ||||||
Grade I | 58 | 5.8% | 54 | 6.8% | 4 | 2.0% |
Grade II | 92 | 9.2% | 85 | 10.7% | 7 | 3.4% |
Grade III | 284 | 28.4% | 216 | 27.1% | 68 | 33.5% |
Grade IV | 566 | 56.6% | 442 | 55.5% | 124 | 61.1% |
Histological subtype | ||||||
Central osteosarcoma | 45 | 4.5% | 42 | 5.3% | 3 | 1.5% |
Chondroblastic osteosarcoma | 151 | 15.1% | 118 | 14.8% | 33 | 16.3% |
Fibroblastic osteosarcoma | 59 | 5.9% | 46 | 5.8% | 13 | 6.4% |
High-grade surface osteosarcoma | 9 | .9% | 7 | .9% | 2 | 1.0% |
Osteosarcoma, NOS | 599 | 59.9% | 458 | 57.5% | 141 | 69.5% |
Parosteal osteosarcoma | 67 | 6.7% | 62 | 7.8% | 5 | 2.5% |
Periosteal osteosarcoma | 19 | 1.9% | 17 | 2.1% | 2 | 1.0% |
Others | 51 | 5.1% | 47 | 5.9% | 4 | 2.0% |
AJCC T staging | ||||||
T1 | 445 | 44.5% | 367 | 46.0% | 78 | 38.4% |
T2 | 534 | 53.4% | 418 | 52.4% | 116 | 57.1% |
T3 | 21 | 2.1% | 12 | 1.5% | 9 | 4.4% |
Radiation | ||||||
Beam radiation | 51 | 5.1% | 31 | 3.9% | 20 | 9.9% |
Combination of beam with implants or isotopes | 2 | 0.2% | 2 | 0.3% | 0 | 0.0% |
None/Unknown | 947 | 94.7% | 764 | 95.9% | 183 | 90.1% |
Chemotherapy | ||||||
No/Unknown | 170 | 17.0% | 143 | 17.9% | 27 | 13.3% |
Yes | 830 | 83.0% | 654 | 82.1% | 176 | 86.7% |
Survival time | ||||||
Mean | 46.8 | 51.1 | 29.7 | |||
Median (range) | 40.0 (0–119.0) | 41.0 (0–119.0) | 25.0 (3.0–89.0) | |||
Marital status | ||||||
Married | 209 | 20.9% | 145 | 18.2% | 64 | 31.5% |
Single/separated/divorced/widowed | 791 | 79.1% | 652 | 81.8% | 139 | 68.5% |
9th grade education | ||||||
Lower 50% | 480 | 48.0% | 393 | 49.3% | 87 | 42.9% |
Upper 50% | 520 | 52.0% | 404 | 50.7% | 116 | 57.1% |
High school education | ||||||
Lower 50% | 496 | 49.6% | 397 | 49.8% | 99 | 48.8% |
Upper 50% | 504 | 50.4% | 400 | 50.2% | 104 | 51.2% |
At least bachelor’s degree | ||||||
Lower 50% | 423 | 42.3% | 335 | 42.0% | 88 | 43.3% |
Upper 50% | 577 | 57.7% | 462 | 58.0% | 115 | 56.7% |
Median family income (in tens) | ||||||
Lower 50% | 496 | 49.6% | 399 | 50.1% | 97 | 47.8% |
Upper 50% | 504 | 50.4% | 398 | 49.9% | 106 | 52.2% |
Unemployed | ||||||
Lower 50% | 459 | 45.9% | 373 | 46.8% | 86 | 42.4% |
Upper 50% | 541 | 54.1% | 424 | 53.2% | 117 | 57.6% |
Families below poverty level | ||||||
Lower 50% | 489 | 48.9% | 392 | 49.2% | 97 | 47.8% |
Upper 50% | 511 | 51.1% | 405 | 50.8% | 106 | 52.2% |
Families above poverty level | ||||||
Lower 50% | 420 | 42.0% | 335 | 42.0% | 85 | 41.9% |
Upper 50% | 580 | 58.0% | 462 | 58.0% | 118 | 58.1% |
OS – overall survival; CSS – cause-specific survival; AJCC – American Joint Committee on Cancer.
Supplementary Table 2.
Univariate analysis results between age and histopathological grade.
Grade | Age | OR | 95% CI | P value |
---|---|---|---|---|
Grade II | 0–14 | 1.00 (reference) | ||
15–39 | 1.167 | 0.426–3.197 | 0.764 | |
>39 | 0.939 | 0.329–2.680 | 0.907 | |
Grade III | 0–14 | 1.00 (reference) | ||
15–39 | 0.415 | 0.181–0.950 | 0.037* | |
>39 | 0.219 | 0.091–0.525 | 0.001* | |
Grade IV | 0–14 | 1.00 (reference) | ||
15–39 | 0.380 | 0.170–0.851 | 0.019* | |
>39 | 0.167 | 0.072–0.388 | <0.001* |
Grade I is the reference group. OR – odds ratio; CI – confidence interval.
P<0.05.
Kaplan-Meier curves of overall survival (OS) (left) and cause-specific survival (CSS) (right) for age (A, B) and grade (C, D).
Kaplan-Meier curves of overall survival (OS) (left) and cause-specific survival (CSS) (right) for histological subtype (A, B) and location subtype (C, D). ICD – International Classification of Diseases; O, NOS – osteosarcoma, No other specific; C – central osteosarcoma; Cb – chondroblastic osteosarcoma; Fb – fibroblastic osteosarcoma; Hs – high-grade surface osteosarcoma; Iw – intraosseous well-differentiated osteosarcoma; Pa – parosteal osteosarcoma; Pe – periosteal osteosarcoma; Sc – small cell osteosarcoma; T – telangiectatic osteosarcoma; Other – small cell osteosarcoma, telangiectatic osteosarcoma and intraosseous well-differentiated osteosarcoma.
Kaplan-Meier curves of overall survival (OS) (left) and cause-specific survival (CSS) (right) for sixth (A, B) and seventh (C, D) edition American Joint Committee on Cancer (AJCC) staging systems.
Supplementary Table 3.
Univariate analysis results between age and American Joint Committee on Cancer T staging.
AJCC T | Age | OR | 95% CI | P value |
---|---|---|---|---|
T2 | 0–14 | 1.00 (reference) | ||
15–39 | 0.984 | 0.736–1.316 | 0.913 | |
>39 | 0.651 | 0.454–0.933 | 0.019* | |
T3 | 0–14 | 1.00 (reference) | ||
15–39 | 0.316 | 0.116–0.864 | 0.025* | |
>39 | 0.306 | 0.084–1.113 | 0.072 |
T1 is the reference group. OR – odds ratio; CI – confidence interval; AJCC – American Joint Committee on Cancer.
P<0.05.