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. 2019 Jun 26;10:1434. doi: 10.3389/fmicb.2019.01434

Structural Diversity and Bioactivities of Peptaibol Compounds From the Longibrachiatum Clade of the Filamentous Fungal Genus Trichoderma

Tamás Marik 1, Chetna Tyagi 1, Dóra Balázs 1, Péter Urbán 2, Ágnes Szepesi 3, László Bakacsy 3, Gábor Endre 1, Dávid Rakk 1, András Szekeres 1, Maria A Andersson 4, Heidi Salonen 4, Irina S Druzhinina 5,6, Csaba Vágvölgyi 1, László Kredics 1,*
PMCID: PMC6606783  PMID: 31293557

Abstract

This study examined the structural diversity and bioactivity of peptaibol compounds produced by species from the phylogenetically separated Longibrachiatum Clade of the filamentous fungal genus Trichoderma, which contains several biotechnologically, agriculturally and clinically important species. HPLC-ESI-MS investigations of crude extracts from 17 species of the Longibrachiatum Clade (T. aethiopicum, T. andinense, T. capillare, T. citrinoviride, T. effusum, T. flagellatum, T. ghanense, T. konilangbra, T. longibrachiatum, T. novae-zelandiae, T. pinnatum, T. parareesei, T. pseudokoningii, T. reesei, T. saturnisporum, T. sinensis, and T. orientale) revealed several new and recurrent 20-residue peptaibols related to trichobrachins, paracelsins, suzukacillins, saturnisporins, trichoaureocins, trichocellins, longibrachins, hyporientalins, trichokonins, trilongins, metanicins, trichosporins, gliodeliquescins, alamethicins and hypophellins, as well as eight 19-residue sequences from a new subfamily of peptaibols named brevicelsins. Non-ribosomal peptide synthetase genes were mined from the available genome sequences of the Longibrachiatum Clade. Their annotation and product prediction were performed in silico and revealed full agreement in 11 out of 20 positions regarding the amino acids predicted based on the signature sequences and the detected amino acids incorporated. Molecular dynamics simulations were performed for structural characterization of four selected peptaibol sequences: paracelsins B, H and their 19-residue counterparts brevicelsins I and IV. Loss of position R6 in brevicelsins resulted in smaller helical structures with higher atomic fluctuation for every residue than the structures formed by paracelsins. We observed the formation of highly bent, almost hairpin-like, helical structures throughout the trajectory, along with linear conformation. Bioactivity tests were performed on the purified peptaibol extract of T. reesei on clinically and phytopathologically important filamentous fungi, mammalian cells, and Arabidopsis thaliana seedlings. Porcine kidney cells and boar spermatozoa proved to be sensitive to the purified peptaibol extract. Peptaibol concentrations ≥0.3 mg ml−1 deterred the growth of A. thaliana. However, negative effects to plants were not detected at concentrations below 0.1 mg ml−1, which could still inhibit plant pathogenic filamentous fungi, suggesting that those peptaibols reported here may have applications for plant protection.

Keywords: Trichoderma, Longibrachiatum, peptaibol, brevicelsin, mass spectrometry, antifungal activity, Arabidopsis, mammalian cells

Introduction

At present, more than 300 species of the genus Trichoderma (Ascomycota, Hypocreales, Hypocreaceae) have been described (Bissett et al., 2015; Zhang and Zhuang, 2018). The majority of these species were described after the year 2000, as only a few species were initially included in the genus (Bisby, 1939; Rifai, 1969). Section Longibrachiatum of the genus was one of the five Trichoderma sections according to Bissett (1984, 1991a,b,c). It forms a monophyletic group phylogenetically separated from the other four Trichoderma sections (Kuhls et al., 1997; Samuels et al., 1998) and is designated recently as the Longibrachiatum Clade (Samuels et al., 2012). It is one of the youngest clades of the genus (Kubicek et al., 2011) and has the largest number of available whole-genome sequence data. This clade is ecologically highly versatile as it contains prominent clinically relevant and ecologically restricted species. Trichoderma longibrachiatum, T. orientale, and T. citrinoviride are opportunistic human pathogens causing infections, mainly in immunocompromised patients (Kuhls et al., 1999; Kredics et al., 2003; Hatvani et al., 2013). T. longibrachiatum or its transformants have also been suggested for use as biocontrol agents against plant pathogens like Pythium ultimum or members of the Fusarium solani species complex (Migheli et al., 1998; Rojo et al., 2007). T. longibrachiatum and T. orientale are sympatric species but have different reproductive strategies, the former being strictly clonal, whereas the latter recombines sexually (Druzhinina et al., 2008). The cellulase producer T. reesei is also capable of sexual reproduction (Seidl et al., 2009), whereas its sympatric species T. parareesei is genetically isolated and has a clonal lifestyle (Atanasova et al., 2010; Druzhinina et al., 2010). While T. longibrachiatum and T. orientale are cosmopolitan, the related T. pinnatum and T. aethiopicum are rare and restricted species (Druzhinina et al., 2010). Numerous other species, including T. reesei, T. parareesei, T. pseudokoningii, T. sinense, T. effusum, T. konilangbra, T. andinense, or T. novae-zelandiae are also geographically restricted (Druzhinina et al., 2012).

Several secondary metabolites are produced by Trichoderma species from the Longibrachiatum Clade. Probably the best known species is T. reesei, which produces hydrolytic enzymes degrading cellulose or hemicellulose (Harman and Kubicek, 1998; Kubicek et al., 2009). Peptaibols are membrane-active compounds with the ability to aggregate and form ion channels in lipid bilayer membranes. They are usually short peptides of 8–20 residues with non-proteinogenic amino acids and are biosynthesised by non-ribosomal peptide synthetases (NRPSs) (Marahiel, 1997; Marahiel et al., 1997; May et al., 2002; Degenkolb et al., 2003, 2007; Bushley and Turgeon, 2010; Marik et al., 2017b). In the case of NRPSs, a single large protein is responsible for the activation, incorporation and elongation of the peptides. NRPSs can also incorporate non-proteinogenic residues, thus increasing the chemical diversity of the products. The lack of specificity of the recognition sites and the three-dimensional structure of the enzyme lead to the acceptance of closely related residues (such as Vxx vs. Lxx). Consequently, the number of positionally isomeric and homologous peptaibols biosynthesised by a single NRPS can be large. The repair mechanisms, which usually operate during biosynthesis, are also absent in NRPS pathways, thus further increasing the variability of the products. Characteristic residues of peptaibols include α-aminoisobutyric acid (Aib) and isovaline (Iva), as well as 1,2-amino alcohols such as Leuol, Valol, Pheol, Tyrol, Ileol, Alaol, and Prool at the C-terminus (Degenkolb et al., 2008; Stoppacher et al., 2013). Peptaibols usually form short, linear helical structures, several of which aggregate to form ion channels and may damage lipid membranes. Investigation of the structural and dynamic properties of peptaibol molecules is important for the understanding of their biological activities. Computational molecular dynamics-based simulation is a popular technique for investigating a molecule's dynamic behavior and predicting its three-dimensional structure. Peptaibols like trichobrachins (Násztor et al., 2013), harzianins (Putzu et al., 2017), alamethicin (Leitgeb et al., 2007; Kredics et al., 2013), tripleurin (Tyagi et al., 2019), and others have been investigated using such techniques. Knowledge about the structure of peptaibols might also facilitate the design of bioactive peptides for future applications. The characteristic non-proteinogenic amino acid residues of peptaibols (Aib and C-terminal alcohols) can be parameterised quantum-mechanically, and the effects of their presence can be evaluated. In general, long molecular time scales are required to effectively simulate peptide folding processes. An all-atom enhanced sampling technique known as accelerated molecular dynamics (aMD) can be used, which provides a non-negative boost to the potential energy and speeds up the process of peptide folding.

Trichoderma species are widely used against various plant pathogenic fungi as biocontrol agents because of their fast growth and reproduction, their mycoparasitism and their production of secondary metabolites (Chaverri et al., 2015; Degenkolb et al., 2015; Waghunde et al., 2016). Species like T. viride, T. virens, T. atroviride, T. asperellum, and T. harzianum are frequently studied due to their production of enzymes and antibiotics valuable in agriculture (Schuster and Schmoll, 2010; Contreras-Cornejo et al., 2016) and their antagonistic effects against pathogenic fungi such as Botrytis cinerea, Alternaria solani and Rhizoctonia solani (Harman et al., 2004). Incubation of a “T. harzianum” strain later re-identified as T. atroviride (Röhrich et al., 2014) with B. cinerea cell walls resulted in the secretion of cell wall hydrolytic enzymes and antibiotic fractions of peptaibols, which inhibited B. cinerea spore germination, causing a fungicidal effect. Peptaibols and hydrolytic enzymes were found to work synergistically in this antagonistic interaction (Schirmböck et al., 1994).

Trichoderma species also interact with plants through secondary metabolites. Although several studies reported positive effects of Trichoderma species on the physiological and biochemical responses of plants (Contreras-Cornejo et al., 2016), inhibition of plant growth and primary root development have also been described (Rippa et al., 2010; Shi et al., 2016). The most thoroughly investigated model plant, Arabidopsis thaliana, is frequently used to test the bioactivity of the secondary metabolites of Trichoderma species (Kottb et al., 2015). Peptaibols can induce auxin production and disruption of the auxin response gradient in root tips (Shi et al., 2016). The most thoroughly studied peptaibol, alamethicin, was shown to induce resistance in plants (Leitgeb et al., 2007; Kredics et al., 2013) but can also be toxic, causing lesions on Arabidopsis leaves (Rippa et al., 2010). However, it should also be considered that the commercially available alamethicin mixture (Sigma-Aldrich A4665) may also contain the trichothecene-type mycotoxin harzianum A produced by the strain T. brevicompactum used for alamethicin fermentations (Degenkolb et al., 2006).

This study aimed at revealing the genomic background, structural diversity and bioactivity of peptaibol compounds produced by different species from the ecologically diverse Longibrachiatum Clade of the genus Trichoderma.

Materials and Methods

Strains and Culture Conditions

Twenty-two strains from 17 Trichoderma species belonging to the Longibrachiatum Clade of the genus were selected from the TU Collection of Industrially Important Microorganisms, Vienna, Austria (TUCIM, www.vt.tuwien.ac.at/tucim/) and the Szeged Microbiology Collection, Szeged, Hungary (SzMC; www.szmc.hu) for investigation of their peptaibol production (Table 1). For testing the antifungal activity of peptaibol extracts, filamentous fungal strains of clinical relevance (Aspergillus fumigatus SzMC 23245, Fusarium falciforme SzMC 11407 and Fusarium keratoplasticum SzMC 11414 from human keratomycosis, India) or phytopathological relevance (Alternaria alternata SzMC 16085, F. solani species complex SzMC 11467 and Phoma cucurbitacearum SzMC 16088) were selected. The strains were maintained and cultured as described by Marik et al. (2017a).

Table 1.

Trichoderma strains from the Longibrachiatum Clade involved in the study.

SzMC identifier Other identifier Subclade* Species Origin References
1773 CECT 2412 Longibrachiatum/Orientale T. longibrachiatum Mushroom compost, Wales Druzhinina et al., 2008
1775 CECT 2937 Longibrachiatum/Orientale T. longibrachiatum Antarctica Kuhls et al., 1997
1776 CECT 20105 Longibrachiatum/Orientale T. longibrachiatum Biocontrol strain, Spain Antal et al., 2005
12546 UAMH 7956 Longibrachiatum/Orientale T. longibrachiatum Bone marrow transplant recipient Richter et al., 1999
12556 UAMH 9573 Longibrachiatum/Orientale T. orientale Peritoneal catheter tip, Canada Kredics et al., 2003
22602 TUCIM 1817 Longibrachiatum/Orientale T. aethiopicum Coffea arabica rhizosphere; Jimma, Ethiopia Druzhinina et al., 2008
22603 TUCIM 3421 Longibrachiatum/Orientale T. pinnatum Sri Lanka Samuels et al., 2012
22614 TUCIM 917, QM6a Parareesei/Reesei T. reesei canvas of US army; Solomon Islands Reese et al., 1950
22616 QM9414 Parareesei/Reesei T. reesei Mutant of QM9123 (which is mutant of QM6a) Kuhls et al., 1996
22617 QM9414 G2Δlae1 Parareesei/Reesei T. reesei lae1 null mutant (Δlae1) of T. reesei QM9414 Seiboth et al., 2012
22615 TUCIM 661 Parareesei/Reesei T. parareesei Subtropical rain forest; Iguazu Falls, Argentina Atanasova et al., 2010
22606 TUCIM 1267 Saturnisporum T. saturnisporum Italy Samuels et al., 2012
22607 TUCIM 132 Konilangbra/Sinensis T. konilangbra Uganda Samuels et al., 1998
22608 TUCIM 3350 Konilangbra/Sinensis T. flagellatum Coffea arabica rhizosphere; Ethiopia Belayneh Mulaw et al., 2010
22609 TUCIM 527 Konilangbra/Sinensis T. sinensis Taiwan Bissett et al., 2003
22618 SJ40 Citrinoviride/Pseudokoningii T. citrinoviride Office bookshelf, settled dust, Espoo, Finland Castagnoli et al., 2018
22613 TUCIM 1277 Citrinoviride/Pseudokoningii T. pseudokoningii the bark of Beilschmiedia tawa Samuels et al., 1998
22612 TUCIM 4158 Novae-zelandiae/ Saturnisporopsis T. novae-zelandiae Native Notophagus forest, New Zealand Samuels et al., 1998
22604 TUCIM 2057 T. ghanense** Agaricus compost; Hungary Hatvani et al., 2007
22605 TUCIM 2883 T. capillare** Wall of a mushroom growing cellar; Hungary Hatvani et al., 2007
22610 TUCIM 1291 T. andinense** Venezuela, high elevation Samuels et al., 1998
22611 TUCIM 254 T. effusum** Soil isolation; Himalaya, India Bissett et al., 2003
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*

Subclades were defined based on Samuels et al. (2012).

**

Considered as lone lineages.

Peptaibol Extraction

Peptaibols were extracted according to Marik et al. (2017a). For large quantity peptaibol production and purification, T. reesei QM9414 (SzMC 22616) was cultured according to Marik et al. (2018). The samples were purified on a Flash chromatograph (CombiFlash EZ Prep UV-VIS Teledyne Isco). The cartridge (CombiFlash EZ Prep) was filled with 60 cm3 silica (30–40 μm), and 1.5 g of crude peptaibol extract was applied above the septum. The flow rate was set to 35 ml min−1 and the wavelength of the UV detector to 270/320 nm. Solvents A and B were chloroform and methanol, respectively (gradient solvent B: 0%, 0 min; 0%, 5 min; 100%, 15 min; 100%, 18 min). Fractions were automatically collected into collector tubes (18 × 180 mm, 30 ml) based on the slope of the UV signal. Fractions were evaporated, dissolved in methanol (100 mg ml−1) and stored at −20°C. The purity of the samples was checked by HPLC-MS as described by Van Bohemen et al. (2016). For this analysis, the appearing y7-ion fragments were quantified and compared to alamethicin (Sigma-Aldrich A-4665, Hungary) dissolved in methanol (VWR, Hungary).

Analytical Procedures and Data Analysis

Crude peptaibol extracts were subjected to HPLC-ESI-MS using a Varian 500 MS equipment with the parameters described previously (Marik et al., 2018). The excitation storage level (m/z)/excitation amplitude (V) conditions during the MS2 measurements of selected y7 fragments were: m/z of 774.4 (209.4/3.02), m/z of 775.4 (209.7/3.03), m/z of 788.4 (212.9/3.08), and m/z of 789.4 (213.2/3.08). The method of peptaibol identification followed the protocol described previously by Marik et al. (2013, 2017a). The initial Varian 500 MS data were further confirmed by HPLC-Orbitrap-MS: Dionex UltiMate 3000 system (Thermo Scientific, CA, USA) controlled by the Xcalibur 4.2 software (Thermo Scientific, CA, USA) and equipped with a quaternary pump, a vacuum degasser, an autosampler and a column heater. Gemini NX-C18 HPLC column (50 × 2.0 mm, 3 μm; Phenomenex Inc., Torrance, CA, USA) was used for the separation. Solvent A was H2O:MeOH:MeCN 8:1:1 with 10 mM ammonium-acetate and 0.1% (v/v) acetic acid, while solvent B was acetonitrile/methanol 1:1 (v/v) with 10 mM ammonium-acetate and 0.1% (v/v) acetic acid. The flow rate was set to 0.2 ml min−1 and the gradient program for Solvent B was 10%−0 min, 10%−2 min, 78%−3 min, 89%−16 min, 95%−16.5 min, 95%−19.5 min, 10%−20 min, 10%−24 min. The column temperature was kept at 30°C and the injection volume was 5 μl. An Orbitrap-MS: Thermo Scientific Q Exactive Plus (Thermo Scientific, CA, USA) with HESI source in positive mode controlled by Xcalibur 4.2 software (Thermo Scientific, CA, USA) was used for the MS measurements. The HESI parameters were: spray voltage−3 kV, sheath gas flow rate−30 arbitrary units, aux gas flow rate−15 arbitrary units, capillary temperature−350°C, aux gas heater−250°C. The acquisition mode was Full-MS-ddMS2. Full-MS paramteres were: resolution−70,000 at m/z 200, AGC target−3e6, maximum injection time−100 ms, scan range−350-2200 m/z. The ddMS2 parameters: fixed first scan at m/z 80, resolution 17500 at m/z 200, AGC target−1e6, maximum injection time−50 ms, isolation window−1 m/z, collusion energy−30 NCE. The minimum AGC target for ddMS2 triggering was 1e5. As no amino acid analysis was carried out for the determination of the Val/Iva and Leu/Ile isomers, the Vxx/Lxx nomenclature was used in the peptaibol sequences. The newly identified peptaibol compounds were named according to the group to which they belong (A or B) and the elution order of the compounds on the HPLC-Varian MS system (I, II, …, n), appended to “Pept.” Compounds with the same retention time but different sequences were considered as variants and named with small latin letters (a, b, …, n; in decreasing order of amount the variants were produced). Group C peptaibols were named as brevicelsins and numbered according to their elution order.

Peptaibol profiles of individual strains were analyzed using cluster analysis in the ClustVis web tool (Metsalu and Vilo, 2015), and a heat map was constructed using the complete linkage and Euclidian distance settings applied to the columns (strains).

Degenkolb et al. (2006) reported that the Sigma alamethicin standard (A-4665) may be contaminated by the trichothecene mycotoxin harzianum A. In the case of the batch used in this study as a reference compound, the detection of harzianum A was carried out based on a previous article (Nielsen et al., 2005). The flow rate was set to 0.2 ml min−1 on a Phenomenex Gemini 50 × 2 mm, 3 μm HPLC column. The column heater was set to 30°C and the injection volume was 5 μl. An Orbitrap-MS detector was attached to the HPLC system and the parameters were set according to the Orbitrap MS parameters described above. The measurements ran in negative ionization mode, the spray voltage was set to −3 kV.

Bioinformatic Analysis of Peptaibol Synthetase Genes

Peptaibol synthetases of Trichoderma species from the Longibrachiatum Clade with accessible full genome sequences, T. reesei, T. parareesei, and T. citrinoviride (GenBank Assembly accession numbers GCA_000167675.2, GCA_001050175.1 and GCA_003025115.1, respectively) and two strains of T. longibrachiatum (GCA_003025155.1, GCA_000332775.1) were identified using the Secondary Metabolites from InterProScan (SMIPS) online software, and 20 as well as 14 module NRPSs were selected (Wolf et al., 2016). In the case of T. longibrachiatum, T. citrinoviride, T. reesei, and T. parareesei, the extracted sequences were analyzed using the Antibiotics and Secondary Metabolites Analysis Shell (antiSMASH), the PKS-NRPS Analysis Web-site, the NRPS/PKS substrate predictor and the NRPSPredictor3 SVM, as described by Marik et al. (2017a).

Accelerated Molecular Dynamics Simulations of 20- and 19-Residue Peptaibols

Calculation of the partial charges for the non-standard residues Aib and Pheol and the preparation of unfolded conformations of four selected peptaibols in water were carried out as described by Tyagi et al. (2019). The Leu and Val positions in brevicelsin sequences were predicted based on their positionally isomeric 20-residue paracelsin counterparts. For the Paracelsin B system, 3910 water molecules were added with a box size of 55.05 × 46.82 × 62.33 Å and a volume of 160676.0 Å3, whereas 3557 TIP3P water molecules were added with a box size of 55.05 × 42.11 × 63.40 Å and a volume of 147021.35 Å3 to prepare the Paracelsin H system. Similarly, 4725 water molecules were added to the Brevicelsin I system with a box size of 67.57 × 50.93 × 54.97 Å and a volume of 189190.34 Å3, whereas 4536 water molecules were added to the Brevicelsin IV system with a box size of 68.52 × 45.96 × 58.30 Å and a volume of 183623.0 Å3.

The four systems were prepared for aMD simulations used to enhance sampling with a boost to the whole potential energy and an extra boost to torsional energy. The values of coefficients a1 and a2 were set to 4, whereas b1 and b2 were set to 0.16, based on previous studies (Pierce et al., 2012).

Peptaibol Bioactivity Assays

For inhibition tests with filamentous fungi, malt extract agar medium completed with yeast extract was used at 25°C, following the method described by Marik et al. (2018). The purified peptaibol extract of T. reesei QM9414 was tested in an agar plate well-diffusion assay with methanol as a control, as well as alamethicin (Sigma-Aldrich A-4665, Hungary) and nystatin (Nystatin 2-hydrate BioChemica, AppliChem A3811,0025, Germany) as reference compounds. All solutions were prepared in two-step dilution series from 0.4 mg ml−1 to 0.0036125 mg ml−1. The inhibition zones were measured as the distance between the edge of the fungal colonies and the edge of the holes containing the peptaibol solutions at the time when the edge of the colony reached the edge of the control hole filled with methanol. At the same time, plates were photographed with a Coolpix S2600 digital camera (Nikon). Minimum inhibitory concentration (MIC) values were defined as the lowest concentrations where an inhibition zone could be detected. Experiments were carried out in triplicate.

In order to investigate the biological effects of peptaibols on plants, A. thaliana (Col-0 ecotype) seeds were planted on 0.5 × Murashige and Skoog agar (8%) medium (Horváth et al., 2015) with the addition of 0.5% sucrose (w/v) (pH adjusted to 5.5 with NaOH) in plastic Petri dishes (90 × 17 mm) five seeds per Petri dish in one line. Seeds were surface sterilized with 70% ethanol for 1 min, treated with 4% hypochlorite for 15 min and washed with sterile distilled water. After vernalisation at 4°C for 24 h, seeds were sown onto the agar plates. Arabidopsis plants were placed in a greenhouse with a photoperiod of 12 h of light and 12 h of darkness, a light intensity of 300 μmol m−2 s−2 and a temperature of 25 ± 1°C. After the third day post germination, plates were placed at an angle of 50° to allow root growth along the agar surface and to promote aerial growth of the hypocotyls. Four 5 mm holes were bored with a sterile cork borer 0.5 cm from the root tips of 5-day-old Arabidopsis seedlings (five seedlings per plate) and filled with 40 μl of peptaibol extract. The growth of primary roots was measured every 24 h for 4 days. Photographs of 15-day-old plants were taken using a Coolpix S2600 digital camera (Nikon). The fresh weights of the plants from each plate were measured, and photosynthetic pigments were quantified as described by Lichtenthaler (1987). Statistical analyses were performed using Bonferroni's multiple comparison tests with the GraphPad Prism software version 6.00 (GraphPad Software, San Diego, CA, USA; www.graphpad.com) using 25 samples.

Bioassays using porcine kidney cells (PK-15) and assays of cell membrane integrity disruption in boar sperm cells were carried out as described previously (Bencsik et al., 2014; Marik et al., 2017b).

Results

Identification of Peptaibols Produced by Trichoderma Species From the Longibrachiatum Clade

Peptaibols produced by species from the Longibrachiatum Clade of genus Trichoderma were identified using the strategy described by Marik et al. (2013, 2017a). Extracted ion chromatograms (EIC) resulting from full scan measurements of crude extracts from the examined Trichoderma strains are shown in Supplementary Figures 1–22. Singly-charged pseudomolecular ions, such as [M+Na]+ or [M+H]+, were scarcely detectable in the spectra, whereas doubly charged ([M+2Na]2+) ions were present and could be used for identification. Full scan MS spectra contained the series of the fragment ions from the N-terminal part (b1—b6 and b8-b13, Supplementary Figure 23) except for b7, where the stable Gln-Aib bond is present in the compounds (Krause et al., 2006a). The C-terminal y7 fragment was consistently observed and provided a good reference for the quantification of the peptides in the mixture. The first 13 amino acid residues could be identified from the full scan MS spectra, but MS2 experiments were performed for the identification of residues at the C-terminus. The last four residues could be identified directly from the MS2 spectra (Supplementary Figure 24). The y7-AA(19-15) ions were not shown on these spectra, therefore another MS2 fragmentation was performed on an Orbitrap-MS system from the y7 ions, which proved Vxx and Aib in positions 15 and 16, respectively (Supplementary Figure 25). All the detected peaks could also be reidentified at high resolution on the HPLC-Orbitrap-MS system, except for y7-H2O (Supplementary Tables 1–6). Instead of [M+Na]+ and [M+2Na]2+ ions, [M+H]+ could be observed on these spectra.

The peptaibol sequences could be categorized into three groups, designated as A (Table 2; Supplementary Tables 1, 4), B (Table 3; Supplementary Tables 2, 5) and C (Table 4; Supplementary Tables 3, 6). Groups A and B contain 20-residue peptaibols, whereas group C sequences had lost a residue in position R6. The novelty of the sequences was validated according to the “Comprehensive Peptaibiotics Database” (Stoppacher et al., 2013) as well as the last, offline version of the “Peptaibiotics Database.” The former online resource (Neumann et al., 2015) is unavailable since the autumn of 2017, therefore PubMed searches of publications since 2017 were performed with the keyword “peptaibol.” Several sequences proved to be homologous or positionally isomeric to the peptaibol subfamilies of trichobrachins, paracelsins, suzukacillins, saturnisporins, trichoaureocins, trichocellins, longibrachins, hyporientalins, trichokonins, trilongins, metanicins, trichosporins, gliodeliquescins, alamethicins, and hypophellins. Some sequences had amino acid exchanges in comparison with previously described compounds from the peptaibol groups listed above.

Table 2.

Sequences of the newly identified group A peptaibol compounds from Trichoderma species of the Longibrachiatum Clade and their similarities to known peptaibols available in the “Comprehensive Peptaibiotics Database.”

Peptide M [M+Na]+ [M+2Na]2+ b13 y7 rt-GK (min) R R1 R2 R3 R4 R5 R6 R7 R8 R9 R10 R11 R12 R13 R14 R15 R16 R17 R18 R19 R20 Compound identical or positionally isomeric with References
Pept-A-Ia 1922 1945 984 1149 774 35.35 Ac Aib Ala Aib Ala Aib Ala Gln Aib Vxx Ala Gly Lxx Aib Pro Vxx Aib Aib Gln Gln Pheol Trichoaureocin 1d Brückner et al., 2002
Pept-A-Ib 1922 1945 984 1149 774 36.88 Ac Aib Ala Aib Ala Aib Aib Gln Aib Vxx Ala Gly Vxx Aib Pro Vxx Aib Aib Gln Gln Pheol New: Trichoaureocin 1d: [Lxx]12 → [Vxx]12 Brückner et al., 2002
Pept-A-IIa 1923 1946 984.5 1149 775 38.26 Ac Aib Ala Aib Ala Aib Ala Gln Aib Vxx Aib Gly Vxx Aib Pro Vxx Aib Aib Glu Gln Pheol New: Longibrachin B II: [Lxx]12 → [Vxx]12 Leclerc et al., 1998
New: Trilongin CI: [Lxx]12 → [Vxx]12 Mikkola et al., 2012
New: Hypophellin 2: [Lxx]12 → [Vxx]12 Röhrich et al., 2013
New: Longibrachin B II; Trilongin CI: [Lxx]12 → [Vxx]12 Tamandegani et al., 2016
Pept-A-IIb 1923 1946 984.5 1149 775 37.46 Ac Aib Ala Aib Ala Aib Ala Gln Aib Vxx Ala Gly Lxx Aib Pro Vxx Aib Aib Glu Gln Pheol New: Trichoaureocin 1d: [Gln]17 → [Glu]17 Brückner et al., 2002
Pept-A-IIIa 1936 1959 991 1149 788 39.82 Ac Aib Ala Aib Ala Aib Ala Gln Aib Vxx Aib Gly Vxx Aib Pro Vxx Aib Vxx Gln Gln Pheol New: Longibrachin A II: [Leu]9 → [Vxx]9 Leclerc et al., 1998
New: Paracelsin F: [Aib]12 → [Vxx]12 Pócsfalvi et al., 1997
New: Suzukacillin A 03: [Aib]12 → [Vxx]12 Krause et al., 2006b
New: Suzukacillin A 10a: [Lxx]12 → [Vxx]12 Krause et al., 2006b
New: Trichoaureocin 4: [Lxx]12 → [Vxx]12 Brückner et al., 2002
New: Trichobrachin II 07, 08, 09 IIb B: [Lxx]12 → [Vxx]12 Krause et al., 2007
New: Trichokonin VII: [Leu]12 → [Vxx]12 Huang et al., 1996
New: Trilongin BII: [Lxx]12 → [Vxx]12 Mikkola et al., 2012
New: Metanicin B: [Leu]12 → [Vxx]12 Kimonyo and Brückner, 2013
New: Hypophellin 3: [Lxx]12 → [Vxx]12 Röhrich et al., 2013
New: Pept-1951-c: [Lxx]12 → [Vxx]12 Tamandegani et al., 2016
New: Hyporientalin A: [Aib]12 → [Vxx]12 Touati et al., 2018
Pept-A-IIIb 1936 1959 991 1149 788 38.17 Ac Aib Ala Aib Ala Aib Ala Gln Aib Vxx Ala Gly Lxx Aib Pro Vxx Aib Vxx Gln Gln Pheol New: Longibrachin A II: [Aib]10 → [Ala]10 Leclerc et al., 1998
New: Suzukacillin A 10a: [Aib]10 → [Ala]10 Krause et al., 2006b
New: Trichoaureocin 4: [Aib]10 → [Ala]10 Brückner et al., 2002
New: Trichoaureocin 1d: [Aib]17 → [Ala]17 Brückner et al., 2002
New: Trichobrachin II 07, 08, 09, IIb B: [Aib]10 → [Ala]10 Krause et al., 2007
New: Trichokonin VII: [Aib]10 → [Ala]10 Huang et al., 1996
New: Trilongin BII: [Aib]10 → [Ala]10 Mikkola et al., 2012
New: Metanicin B: [Aib]10 → [Ala]10 Kimonyo and Brückner, 2013
New: Hypophellin 3: [Aib]10 → [Ala]10 Röhrich et al., 2013
New: Pept-1951-c: [Aib]10 → [Ala]10 Tamandegani et al., 2016
Pept-A-IIIc 1936 1959 991 1149 788 39.89 Ac Aib Ala Aib Ala Aib Aib Gln Aib Vxx Ala Gly Vxx Aib Pro Vxx Aib Vxx Gln Gln Pheol New: Pept-1965-c-1,−2: [Lxx]9 → [Vxx]9 and [Lxx]12 → [Vxx]12 Tamandegani et al., 2016
New: Hyporientalin A: [Aib]10 → [Ala]10 Touati et al., 2018
Pept-A-IVa 1936 1959 991 1163 774 40.21 Ac Aib Ala Aib Ala Aib Ala Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Aib Gln Gln Pheol Longibrachin A I (Positional isomer of Pept-A-VIIa) Leclerc et al., 1998
Trichoaureocin 3 Brückner et al., 2002
Trichobrachin II 05, 06 IIb A Krause et al., 2007
Trichokonin VI Huang et al., 1994
Trilongin BI Mikkola et al., 2012
Metanicin A Kimonyo and Brückner, 2013
Gliodeliquescin A Brückner and Przybylski, 1984
Hypophellin 1 Röhrich et al., 2013
Longibrachin A I, Trilongin BI Tamandegani et al., 2016
Pept-A-IVb 1936 1959 991 1163 774 40.18 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Ala Gly Lxx Aib Pro Vxx Aib Aib Gln Gln Pheol New: Suzukacillin A 11a, 09: [Aib]10 → [Ala]10 Krause et al., 2006b
New: Trichocellin-TC-A-V; -VII: [Aib]10 → [Ala]10 Wada et al., 1994
Pept-A-Va 1950 1973 998 1177 774 40.73 Ac Aib Ala Aib Ala Aib Aib Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Aib Gln Gln Pheol Trichosporin TS-B-IVc (Position isomer of Pept-A-XVIa) Iida et al., 1990
Longibrachin A III Leclerc et al., 1998
Trichoaureocin 5 Brückner et al., 2002
Trichobrachin IIb C Krause et al., 2007
Trichokonin VIII Huang et al., 1996
Trilongin BIII Mikkola et al., 2012
Metanicin C Kimonyo and Brückner, 2013
Hypophellin 5 Röhrich et al., 2013
Longibrachin A III. Tamandegani et al., 2016
Pept-A-Vb 1950 1973 998 1177 774 41.40 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Aib Gly Lxx Aib Pro Vxx Aib Aib Gln Gln Pheol Suzukacillin A 11a, A 09 Krause et al., 2006b.
Trichocellin TC-A-V, TC-A-VII Wada et al., 1994
Pept-A-VIa 1937 1960 991.5 1163 775 41.46 Ac Aib Ala Aib Ala Aib Ala Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Aib Glu Gln Pheol Longibrachin B II Leclerc et al., 1998
Trilongin CI Mikkola et al., 2012
Hypophellin 2 Röhrich et al., 2013
Longibrachin B II., Trilongin CI. Tamandegani et al., 2016
Pept-A-VIb 1937 1960 991.5 1163 775 41.50 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Ala Gly Lxx Aib Pro Vxx Aib Aib Glu Gln Pheol New: Longibrachin B II: [Val]9 → [Lxx]9 and [Aib]10 → [Ala]10 Leclerc et al., 1998
New: Trilongin CI: [Vxx]9 → [Lxx]9 and [Aib]10 → [Ala]10 Mikkola et al., 2012
New: Hypophellin 2: [Vxx]9 → [Lxx]9 and [Aib]10 → [Ala]10 Röhrich et al., 2013
New: Longibrachin B II., Trilongin CI.: [Vxx]9 → [Lxx]9 and [Aib]10 → [Ala]10 Tamandegani et al., 2016
New: Trichocellin TC-B-I: [Aib]10 → [Ala]10 and [Aib]12 → [Lxx]12 Wada et al., 1994
Pept-A-VIIa 1936 1959 991 1163 774 41.00 Ac Aib Ala Aib Ala Aib Ala Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Aib Gln Gln Pheol (Positional isomer of Pept-A-IVa) → Pept-A-IVa
Pept-A-VIIb 1936 1959 991 1163 774 42.53 Ac Aib Ala Vxx Ala Aib Ala Gln Aib Vxx Ala Gly Lxx Aib Pro Vxx Aib Aib Gln Gln Pheol New: Trichoaureocin 1d: [Aib]3 → [Vxx]3 Brückner et al., 2002
Pept-A-VIIIa 1950 1973 998 1177 774 42.29 Ac Aib Ala Vxx Ala Aib Ala Gln Aib Lxx Ala Gly Lxx Aib Pro Vxx Aib Aib Gln Gln Pheol New: Suzukacillin A 11a, 09: [Aib]3 → [Vxx]3and [Aib]10 → [Ala]10 Krause et al., 2006b
New: Trichocellin-TC-A-V; -VII: [Aib]3 → [Vxx]3 and [Aib]10 → [Ala]10 Wada et al., 1994
New: Trichoaureocin 1d: [Aib]3 → [Vxx]3 and [Val]9 → [Lxx]9 (Brückner et al., 2002)
Pept-A-VIIIb 1950 1973 998 1177 774 42.46 Ac Aib Ala Vxx Ala Aib Ala Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Aib Gln Gln Pheol New: Longibrachin A I: [Aib]3 → [Vxx]3 Leclerc et al., 1998
New: Trichoaureocin 3: [Ala]3 → [Vxx]3 Brückner et al., 2002
New: Trichobrachin II 03: [Aib]3 → [Vxx]3 Krause et al., 2007
New: Trichobrachin II 05, 06 IIb A: [Aib]3 → [Vxx]3 Krause et al., 2007
New: Trichokonin IIc: [Ala]3 → [Vxx]3 Huang et al., 1996
New: Trichokonin VI: [Aib]3 → [Vxx]3 Huang et al., 1994
New: Trilongin BI: [Aib]3 → [Vxx]3 Mikkola et al., 2012
New: Metanicin A: [Aib]3 → [Vxx]3 Kimonyo and Brückner, 2013
New: Gliodeliquescin A: [Aib]3 → [Vxx]3 Brückner and Przybylski, 1984
New: Hypophellin 1: [Aib]3 → [Vxx]3 Röhrich et al., 2013
New: Longibrachin A I, Trilongin BI: [Aib]3 → [Vxx]3 Tamandegani et al., 2016
Pept-A-IXa 1950 1973 998 1163 788 42.76 Ac Aib Ala Aib Ala Aib Ala Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Vxx Gln Gln Pheol Longibrachin A II (Position isomer of Pept-A-XVa and Pept-A-XVIIb) Leclerc et al., 1998
Suzukacillin A 10a Krause et al., 2006b
Trichoaureocin 4 Brückner et al., 2002
Trichobrachin II 07, 08, 09, IIb B Krause et al., 2007
Trichokonin VII Huang et al., 1996
Trilongin BII Mikkola et al., 2012
Metanicin B Kimonyo and Brückner, 2013
Hypophellin 3 Röhrich et al., 2013
Pept-1951-c Tamandegani et al., 2016
Hyporientalin A Touati et al., 2018
Pept-A-IXb 1950 1973 998 1163 788 42.84 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Ala Gly Lxx Aib Pro Vxx Aib Vxx Gln Gln Pheol New: Suzukacillin A 10b, 11b, 13: [Aib]10 → [Ala]10 Krause et al., 2006b
New: Trichocellin TC-A-VI, TC-A-VIII: [Aib]10 → [Ala]10 Wada et al., 1994
Pept-A-Xa 1964 1987 1005 1177 788 43.28 Ac Aib Ala Aib Ala Aib Aib Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Vxx Gln Gln Pheol Longibrachin A IV (Position isomer of Pept-A-XIVb Pept-A-XVIIa, Pept-A-XXIa, and Pept-XXVa) Leclerc et al., 1998
Trichoaureocin 6 Brückner et al., 2002
Trichobrachin II 10, IIb D Krause et al., 2007
Trichokonin IX Huang et al., 1995
Trilongin BIV Mikkola et al., 2012
Metanicin D Kimonyo and Brückner, 2013
Hypophellin 7 Röhrich et al., 2013
Pept-A-Xb 1964 1987 1005 1177 788 42.89 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Aib Gly Lxx Aib Pro Vxx Aib Vxx Gln Gln Pheol Suzukacillin A 10b, 11b, 13 Krause et al., 2006b
Trichocellin TC-A-VI, TC-A-VIII Wada et al., 1994
Pept-A-XIa 1951 1974 998.5 1177 775 43.60 Ac Aib Ala Vxx Ala Aib Ala Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Aib Glu Gln Pheol New: Longibrachin B II: [Aib]3 → [Vxx]3 Leclerc et al., 1998
New: Trilongin CI: [Aib]3 → [Vxx]3 Mikkola et al., 2012
New: Hypophellin 2: [Aib]3 → [Vxx]3 Röhrich et al., 2013
New: Longibrachin B II., Trilongin CI.: [Aib]3 → [Vxx]3 Tamandegani et al., 2016
Pept-A-XIb 1951 1974 998.5 1177 775 43.60 Ac Aib Ala Aib Ala Aib Aib Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Aib Glu Gln Pheol Trilongin CIII (Positional isomer of Pept-A-XIXa) Mikkola et al., 2012
Hypophellin 6 Röhrich et al., 2013
Longibrachin B III., Trilongin CIII. Tamandegani et al., 2016
Pept-A-XIc 1951 1974 998.5 1177 775 43.62 Ac Aib Ala Vxx Ala Aib Ala Gln Aib Lxx Ala Gly Lxx Aib Pro Vxx Aib Aib Glu Gln Pheol New: Longibrachin B II: [Aib]3 → [Vxx]3, [Val]9 → [Lxx]9, and [Aib]10 → [Ala]10 Leclerc et al., 1998
New: Trilongin CI: [Aib]3 → [Vxx]3, [Vxx]9 → [Lxx]9, and [Aib]10 → [Ala]10 Mikkola et al., 2012
New: Hypophellin 2: [Aib]3 → [Vxx]3, [Vxx]9 → [Lxx]9, and [Aib]10 → [Ala]10 Röhrich et al., 2013
New: Longibrachin B II., Trilongin CI.: [Aib]3 → [Vxx]3, [Vxx]9 → [Lxx]9, and [Aib]10 → [Ala]10 Tamandegani et al., 2016
New: Trichocellin TC-B-I: [Aib]3 → [Vxx]3, [Aib]10 → [Ala]10, and [Aib]12 → [Lxx]12 Wada et al., 1994
Pept-A-XII 1937 1960 991.5 1163 775 42.81 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Ala Gly Lxx Aib Pro Vxx Aib Aib Glu Gln Pheol (Positional isomer of Pept-A-VIb) → Pept-A-VIb
Pept-A-XIIIa 1951 1974 998.5 1163 789 44.14 Ac Aib Ala Aib Ala Aib Ala Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Vxx Glu Gln Pheol Longibrachin B III Leclerc et al., 1998
Trilongin CII Mikkola et al., 2012
Hypophellin 4 Röhrich et al., 2013
Pept-1952-d Tamandegani et al., 2016
Longibrachin A II., Trilongin BII. Tamandegani et al., 2016
Pept-A-XIIIb 1951 1974 998.5 1163 789 44.16 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Ala Gly Lxx Aib Pro Vxx Aib Vxx Glu Gln Pheol New: Longibrachin B III: [Val]9 → [Lxx]9 and [Aib]10 → [Ala]10 Leclerc et al., 1998
New: Trilongin CII: [Vxx]9 → [Lxx]9 and [Aib]10 → [Ala]10 Mikkola et al., 2012
New: Hypophellin 4: [Vxx]9 → [Lxx]9 and [Aib]10 → [Ala]10 Röhrich et al., 2013
New: Pept-1952-d: [Vxx]9 → [Lxx]9 and [Aib]10 → [Ala]10 Tamandegani et al., 2016
New: Longibrachin B II., Trilongin CI.: [Vxx]9 → [Lxx]9 and [Aib]10 → [Ala]10 Tamandegani et al., 2016
New: Trichocellin TC-B-II: [Aib]10 → [Ala]10 and [Aib]12 → [Lxx]12 Wada et al., 1994
Pept-A-XIVa 1965 1988 1005.5 1177 789 44.22 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Ala Gly Lxx Aib Pro Vxx Aib Vxx Glu Gln Pheol Pept-1966-d Tamandegani et al., 2016
Pept-A-XIVb 1964 1987 1005 1177 788 44.13 Ac Aib Ala Aib Ala Aib Aib Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Vxx Gln Gln Pheol (Position isomer of Pept-A-Xa, Pept-A-XVIIa, Pept-A-XXIa, and Pept-XXVa) → Pept-A-Xa
Pept-A-XVa 1950 1973 998 1163 788 45.00 Ac Aib Ala Aib Ala Aib Ala Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Vxx Gln Gln Pheol (Position isomer of Pept-A-IXa and Pept-A-XVIIb) → Pept-A-IXa
Pept-A-XVb 1964 1987 1005 1177 788 44.74 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Ala Gly Lxx Aib Pro Vxx Aib Vxx Gln Gln Pheol Pept-1965-c-1, c-2 (Position isomer of Pept-A-XXIb) Tamandegani et al., 2016
Pept-A-XVIa 1950 1973 998 1177 774 45.21 Ac Aib Ala Aib Ala Aib Aib Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Aib Gln Gln Pheol (Position isomer of Pept-A-Va) → Pept-A-Va
Pept-A-XVIb 1950 1973 998 1177 774 45.33 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Ala Gly Lxx Aib Pro Vxx Aib Aib Gln Gln Pheol New: Trichosporin TS-B-VIa: [Aib]10 → [Ala]10 Iida et al., 1990
New: Trichoderma citrinoviride sequence 7: [Aib]10 → [Ala]10 Maddau et al., 2009
New: Pept-1965-c-1, c-2: [Vxx]17 → [Aib]17 Tamandegani et al., 2016
Pept-A-XVIIa 1964 1987 1005 1177 788 46.21 Ac Aib Ala Aib Ala Aib Aib Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Vxx Gln Gln Pheol (Position isomer of Pept-A-Xa, Pept-A-XIVb, Pept-A-XXIa, and Pept-XXVa) → Pept-A-Xa
Pept-A-XVIIb 1950 1973 998 1163 788 46.18 Ac Aib Ala Aib Ala Aib Ala Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Vxx Gln Gln Pheol (Position isomer of Pept-A-XVa and Pept-A-IXa) → Pept-A-IXa
Pept-A-XVIII 1978 2001 1012 1191 788 46.36 Ac Aib Ala Vxx Ala Aib Aib Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Vxx Gln Gln Pheol New: Trichosporin TS-B-IVd: [Ala]3 → [Vxx]3 (Position isomer of Pept-A-XXIV and Pept-XXVI) Iida et al., 1990
New: Longibrachin A IV: [Aib]3 → [Vxx]3 Leclerc et al., 1998
New: Trichoaureocin 6: [Aib]3 → [Vxx]3 Brückner et al., 2002
New: Trichobrachin II 10, IIb D: [Aib]3 → [Vxx]3 Krause et al., 2007
New: Trichokonin IX: [Aib]3 → [Vxx]3 Huang et al., 1995
New: Trilongin BIV: [Aib]3 → [Vxx]3 Mikkola et al., 2012
New: Metanicin D: [Aib]3 → [Vxx]3 Kimonyo and Brückner, 2013
New: Hypophellin 7: [Aib]3 → [Vxx]3 Röhrich et al., 2013
Pept-A-XIXa 1951 1974 998.5 1177 775 46.67 Ac Aib Ala Aib Ala Aib Aib Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Aib Glu Gln Pheol (Positional isomer of Pept-A-XIb) → Pept-A-XIb
Pept-A-XIXb 1951 1974 998.5 1177 775 46.86 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Ala Gly Lxx Aib Pro Vxx Aib Aib Glu Gln Pheol New: Trichosporin TS-B-VIa: [Aib]10 → [Ala]10 and [Gln]18 → [Glu]18 Iida et al., 1990
New: Trichoderma citrinoviride sequence 7, [Aib]10 → [Ala]10 and [Gln]18 → [Glu]18 Maddau et al., 2009
New: Pept-1965-c-1, c-2: [Vxx]17 → [Aib]17 and [Gln]18 → [Glu]18 Tamandegani et al., 2016
Pept-A-XX 1964 1987 1005 1191 774 47.30 Ac Aib Ala Vxx Ala Aib Aib Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Aib Gln Gln Pheol New: Trichosporin TS-B-IVc: [Aib]3 → [Vxx]3 (Position isomer of Pept-A-XXIIa) Iida et al., 1990
New: Longibrachin A III: [Aib]3 → [Vxx]3 Leclerc et al., 1998
New: Trichoaureocin 5: [Aib]3 → [Vxx]3 Brückner et al., 2002
New: Trichobrachin, IIb C: [Aib]3 → [Vxx]3 Krause et al., 2007
New: Trichokonin VIII: [Aib]3 → [Vxx]3 Huang et al., 1996
New: Trichosporin TS-B-IIId: [Ala]3 → [Vxx]3 Iida et al., 1990
New: Trilongin BIII: [Aib]3 → [Vxx]3 Mikkola et al., 2012
New: Metanicin C: [Aib]3 → [Vxx]3 Kimonyo and Brückner, 2013
New: Hypophellin 5: [Aib]3 → [Vxx]3 Röhrich et al., 2013
New: Longibrachin A III.: [Aib]3 → [Vxx]3 Tamandegani et al., 2016
Pept-A-XXIa 1964 1987 1005 1177 788 47.85 Ac Aib Ala Aib Ala Aib Aib Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Vxx Gln Gln Pheol (Position isomer of Pept-A-Xa, Pept-A-XIVb, Pept-A-XVIIa, and Pept-XXVa) → Pept-A-Xa
Pept-A-XXIb 1964 1987 1005 1177 788 47.75 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Ala Gly Lxx Aib Pro Vxx Aib Vxx Gln Gln Pheol (Position isomer of Pept-A-XVb) → Pept-A-XVb
Pept-A-XXIIa 1964 1987 1005 1191 774 48.93 Ac Aib Ala Vxx Ala Aib Aib Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Aib Gln Gln Pheol New: (Position isomer of Pept-A-XX) → Pept-A-XX
Pept-A-XXIIb 1964 1987 1005 1191 774 48.79 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Lxx Aib Pro Vxx Aib Aib Gln Gln Pheol Trichosporin TS-B-VIa Iida et al., 1990
Trichoderma citrinoviride sequence 7 Maddau et al., 2009
Pept-A-XXIII 1965 1988 1005.5 1177 789 49.13 Ac Aib Ala Aib Ala Aib Aib Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Vxx Glu Gln Pheol Trilongin CIV (Positional isomer of Pept-A-XXVIIa) Mikkola et al., 2012
Hypophellin 8 Röhrich et al., 2013
Pept-A-XXIV 1978 2001 1012 1191 788 49.89 Ac Aib Ala Vxx Ala Aib Aib Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Vxx Gln Gln Pheol (Position isomer of Pept-A-XVIII and Pept-XXVI) → Pept-A-XVIII
Pept-A-XXVa 1964 1987 1005 1177 788 49.65 Ac Aib Ala Aib Ala Aib Aib Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Vxx Gln Gln Pheol (Position isomer of Pept-A-Xa, Pept-A-XIVb, Pept-A-XVIIa, and Pept-XXIa) → Pept-A-Xa
Pept-A-XXVb 1978 2001 1012 1191 788 49.72 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Lxx Aib Pro Vxx Aib Vxx Gln Gln Pheol Suzukacillin A 12 (Position isomer of Pept-A-XXVIb and Pept-XXVIIb) Krause et al., 2006b
Pept-A-XXVIa 1978 2001 1012 1191 788 51.29 Ac Aib Ala Vxx Ala Aib Aib Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Vxx Gln Gln Pheol (Position isomer of Pept-A-XVIII and Pept-XXIV) → Pept-A-XVIII
Pept-A-XXVIb 1978 2001 1012 1191 788 50.85 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Lxx Aib Pro Vxx Aib Vxx Gln Gln Pheol (Position isomer of Pept-A-XXVb and Pept-XXVIIb) → Pept-A-XVb
Pept-A-XXVIIa 1965 1988 1005.5 1177 789 51.44 Ac Aib Ala Aib Ala Aib Aib Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Vxx Glu Gln Pheol (Positional isomer of Pept-A-XXIII) → Pept-A-XXIII
Pept-A-XXVIIb 1978 2001 1012 1191 788 51.59 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Lxx Aib Pro Vxx Aib Vxx Gln Gln Pheol (Position isomer of Pept-A-XXVb and Pept-XXVIb) → Pept-A-XVb
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Variable residues are UNDERLINED in the table header; minor sequence variants are UNDERLINED in the sequences. Amino acid exchanges in new compounds are set in italic.

Table 3.

Sequences of the newly identified group B peptaibol compounds from Trichoderma species of the Longibrachiatum Clade and their similarities to known peptaibols available in the “Comprehensive Peptaibiotics Database.”

Peptide M [M+Na]+ [M+2Na]2+ b13 y7 rt-GK (min) R R1 R2 R3 R4 R5 R6 R7 R8 R9 R10 R11 R12 R13 R14 R15 R16 R17 R18 R19 R20 Compound identical or positionally isomeric with References
Pept-B-I 1908 1931 977 1135 774 22.59 Ac Aib Ala Ala Ala Aib Ala Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol New: Paracelsin B: [Aib]3 → [Ala]3 (Positional isomer of Pept-B-II, III, and V) Pócsfalvi et al., 1997
New: Saturnisporin SA I: [Aib]3 → [Ala]3 Rebuffat et al., 1993
New: Suzukacillin A 02, A 06: [Aib]3 → [Ala]3 Krause et al., 2006b
New: Trichocellin TC-A-I, TC-A-III: [Aib]3 → [Ala]3 Wada et al., 1994
Pept-B-II 1908 1931 977 1135 774 24.79 Ac Aib Ala Ala Ala Aib Ala Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol New: (Positional isomer of Pept-B-I, III, and V) → Pept-B-I
Pept-B-III 1908 1931 977 1135 774 25.62 Ac Aib Ala Ala Ala Aib Ala Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol New: (Positional isomer of Pept-B-I, II, and V) → Pept-B-I
Pept-B-IV 1922 1945 984 1135 788 25.72 Ac Aib Ala Ala Ala Aib Ala Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol New: Paracelsin H: [Aib]3 → [Ala]3 (Positional isomer of Pept-B-VII) Pócsfalvi et al., 1997
New: Saturnisporin SA II: [Aib]3 → [Ala]3 Rebuffat et al., 1993
New: Suzukacillin A 04, A 08: [Aib]3 → [Ala]3 Krause et al., 2006b
New: Trichocellin TC-A-II, TC-A-IV: [Aib]3 → [Ala]3 Wada et al., 1994
Pept-B-V 1908 1931 977 1135 774 26.35 Ac Aib Ala Ala Ala Aib Ala Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol New: (Positional isomer of Pept-B-I, II, and III) → Pept-B-I
Pept-B-VI 1922 1945 984 1149 774 27.22 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol Paracelsin B (Positional isomer of Pept-B-XII, XVIII, and XXIII) Pócsfalvi et al., 1997
Saturnisporin SA I Rebuffat et al., 1993
Suzukacillin A 02, A 06 Krause et al., 2006b
Trichocellin TC-A-I, TC-A-III Wada et al., 1994
Pept-B-VII 1922 1945 984 1135 788 27.80 Ac Aib Ala Ala Ala Aib Ala Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol New: (Positional isomer of Pept-B-IV) → Pept-B-IV
Pept-B-VIII 1936 1959 991 1149 788 27.27 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol Paracelsin H (Positional isomer of Pept-B-XVII, XIX, XXII, and XXIXb) Pócsfalvi et al., 1997
Saturnisporin SA II Rebuffat et al., 1993
Suzukacillin A 04, A 08 Krause et al., 2006b
Trichocellin TC-A-II, TC-A-IV Wada et al., 1994
Pept-B-IXa 1908 1931 977 1135 774 28.44 Ac Aib Ala Aib Ala Aib Ala Gln Aib Vxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol Paracelsin A Pócsfalvi et al., 1997
Suzukacillin A 01 Krause et al., 2006b
Pept-B-IXb 1908 1931 977 1135 774 28.38 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Aib Gly Ala Aib Pro Vxx Aib Aib Gln Gln Pheol New: Trichoderma citrinoviride sequence 1: [Vxx]17 → [Aib]17 Maddau et al., 2009
Pept-B-X 1922 1945 984 1135 788 28.77 Ac Aib Ala Aib Ala Aib Ala Gln Aib Vxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol Paracelsin F (Positional isomer of Pept-B-XI, XIII, and XVa) Pócsfalvi et al., 1997
Suzukacillin A 03 Krause et al., 2006b
Pept-B-XI 1922 1945 984 1135 788 29.25 Ac Aib Ala Aib Ala Aib Ala Gln Aib Vxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol (Positional isomer of Pept-B-X, XIII, and XVa) → Pept-B-X
Pept-B-XII 1922 1945 984 1149 774 29.90 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol (Positional isomer of Pept-B-VI, XVIII, and XXIII) → Pept-B-VI
Pept-B-XIII 1922 1945 984 1135 788 30.28 Ac Aib Ala Aib Ala Aib Ala Gln Aib Vxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol (Positional isomer of Pept-B-X, XI, and XVa) → Pept-B-X
Pept-B-XIVa 1923 1946 984.5 1149 775 31.36 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Glu Gln Pheol Trichocellin TC-B-I Wada et al., 1994
Pept-B-XIVb 1923 1946 984.5 1149 775 31.40 Ac Aib Ala Aib Ala Aib Aib Gln Aib Vxx Aib Gly Aib Aib Pro Vxx Aib Aib Glu Gln Pheol New: Trichocellin TC-B-I: [Ala]6 → [Aib]6 and [Leu]9 → [Vxx]9 Wada et al., 1994
Pept-B-XVa 1922 1945 984 1135 788 31.48 Ac Aib Ala Aib Ala Aib Ala Gln Aib Vxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol (Positional isomer of Pept-B-X, XI, and -XIII) → Pept-B-X
Pept-B-XVb 1922 1945 984 1135 788 31.53 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Aib Gly Ala Aib Pro Vxx Aib Vxx Gln Gln Pheol Trichoderma citrinoviride sequence 1 Maddau et al., 2009
Pept-B-XVI 1922 1945 984 1149 774 31.98 Ac Aib Ala Aib Ala Aib Aib Gln Aib Vxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol Paracelsin C Pócsfalvi et al., 1997
Pept-B-XVII 1936 1959 991 1149 788 32.67 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol (Positional isomer of Pept-B-VIII, XIX, XXII, and XXIXb) → Pept-B-VIII
Pept-B-XVIII 1922 1945 984 1149 774 33.49 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol (Positional isomer of Pept-B-VI, XII, and XXIII) → Pept-B-VI
Pept-B-XIX 1936 1959 991 1149 788 33.55 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol (Positional isomer of Pept-B-VIII, XVII, XXII, and XXIXb) → Pept-B-VIII
Pept-B-XX 1936 1959 991 1163 774 34.41 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol Paracelsin D (Positional isomer of Pept-B-XXXIIIa, XXXVa, XLIIb, XLVIa, and LVIII) Pócsfalvi et al., 1997
Saturnisporin SA III Rebuffat et al., 1993
Suzukacillin A 05 Krause et al., 2006b
Pept-B-XXI 1937 1960 991.5 1149 789 34.15 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Glu Gln Pheol Trichocellin TC-B-II Wada et al., 1994
Pept-B-XXII 1936 1959 991 1149 788 34.59 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol (Positional isomer of Pept-B-VIII, XVII, XIX, and XXIXb) → Pept-B-VIII
Pept-B-XXIII 1922 1945 984 1149 774 35.25 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol (Positional isomer of Pept-B-VI, XII, and XVIII) → Pept-B-VI
Pept-B-XXIV 1950 1973 998 1163 788 35.59 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol Saturnisporin SA IV (Positional isomer of Pept-B-XXVII, XXXIIa, XXXVIII, and XLVa) Rebuffat et al., 1993
Suzukacillin A 07 Krause et al., 2006b
Pept-B-XXV 1937 1960 991.5 1163 775 35.97 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Glu Gln Pheol New: Trichocellin TC-B-I: [Ala]6 → [Aib]6 (Positional isomer of Pept-B-XXXVII) Wada et al., 1994
New: Paracelsin D: [Gln]18 → [Glu]18 Pócsfalvi et al., 1997
New: Saturnisporin SA III: [Gln]18 → [Glu]18 Rebuffat et al., 1993
New: Suzukacillin A 05: [Gln]18 → [Glu]18 Krause et al., 2006b
Pept-B-XXVI 1950 1973 998 1177 774 36.65 Ac Aib Ala Vxx Ala Aib Aib Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol New: Paracelsin D: [Aib]3 → [Vxx]3 Pócsfalvi et al., 1997
New: Saturnisporin SA III: [Aib]3 → [Vxx]3 Rebuffat et al., 1993
New: Suzukacillin A 05: [Aib]3 → [Vxx]3 Krause et al., 2006b
Pept-B-XXVII 1950 1973 998 1163 788 37.31 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol (Positional isomer of Pept-B-XXIV, XXXIIa, XXXVIII, and XLVa) → Pept-B-XXIV
Pept-B-XXVIII 1950 1973 998 1177 774 37.89 Ac Aib Ala Aib Ala Vxx Aib Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol New: Paracelsin D: [Aib]5 → [Vxx]5 Pócsfalvi et al., 1997
New: Saturnisporin SA III: [Aib]5 → [Vxx]5 Rebuffat et al., 1993
New: Suzukacillin A 05: [Aib]5 → [Vxx]5 Krause et al., 2006b
Pept-B-XXIXa 1936 1959 991 1149 788 38.30 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Ala Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol New: Paracelsin D: [Aib]10 → [Ala]10 Pócsfalvi et al., 1997
New: Saturnisporin SA III: [Aib]10 → [Ala]10 Rebuffat et al., 1993
New: Suzukacillin A 05: [Aib]10 → [Ala]10 Krause et al., 2006b
Pept-B-XXIXb 1936 1959 991 1149 788 37.80 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol (Positional isomer of Pept-B-VIII, XVII, XIX, and XXII) → Pept-B-VIII
Pept-B-XXX 1950 1973 998 1177 774 38.51 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Vxx Aib Pro Vxx Aib Aib Gln Gln Pheol Trichoderma citrinoviride sequence 4 (Positional isomer of Pept-B-XXXIIIc, XLIIa, XLVIb, and LIII) Maddau et al., 2009
Pept-B-XXXI 1951 1974 998.5 1163 789 39.13 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Glu Gln Pheol New: Trichocellin TC-B-II: [Ala]6 → [Aib]6 (Positional isomer of Pept-B-XXXIVb and LII) Wada et al., 1994
New: Saturnisporin SA IV: [Gln]18 → [Glu]18 Rebuffat et al., 1993
New: Suzukacillin A 07: [Gln]18 → [Glu]18 Krause et al., 2006b
Pept-B-XXXIIa 1950 1973 998 1163 788 39.15 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol (Positional isomer of Pept-B-XXIV, XXVII, XXXVIII, and XLVa) → Pept-B-XXIV
Pept-B-XXXIIb 1964 1987 1005 1177 788 39.20 Ac Aib Ala Aib Ala Aib Vxx Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol New: Paracelsin H: [Ala]6 → [Vxx]6 (Positional isomer of Pept-B-XLIb) Pócsfalvi et al., 1997
New: Saturnisporin SA II: [Ala]6 → [Vxx]6 Rebuffat et al., 1993
New: Saturnisporin SA IV: [Aib]6 → [Vxx]6 Rebuffat et al., 1993
New: Suzukacillin A 04, 08: [Ala]6 → [Vxx]6 Krause et al., 2006b
New: Suzukacillin A 07: [Ala]6 → [Vxx]6 Krause et al., 2006b
New: Trichocellin TC-A-II, TC-A-IV: [Ala]6 → [Vxx]6 Wada et al., 1994
Pept-B-XXXIIIa 1936 1959 991 1163 774 38.98 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol (Positional isomer of Pept-B-XX, XXXVa, XLIIb, XLVIa, and LVIII) → Pept-B-XX
Pept-B-XXXIIIb 1936 1959 991 1163 774 39.25 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Aib Gly Vxx Aib Pro Vxx Aib Aib Gln Gln Pheol Trichoderma citrinoviride sequence 2 (Poitional isomer of Pept-XXXVb) Maddau et al., 2009
Pept-B-XXXIIIc 1950 1973 998 1177 774 39.20 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Vxx Aib Pro Vxx Aib Aib Gln Gln Pheol (Positional isomer of Pept-B-XXX, XLIIa, XLVIb, and LIII) → Pept-B-XXX
Pept-B-XXXIIId 1951 1974 998.5 1177 775 39.31 Ac Aib Ala Aib Ala Vxx Aib Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Glu Gln Pheol New: Paracelsin D: [Aib]5 → [Vxx]5 and [Aib]18 → [Glu]18 Pócsfalvi et al., 1997
New: Saturnisporin SA III: [Aib]5 → [Vxx]5 and [Aib]18 → [Glu]18 Rebuffat et al., 1993
New: Suzukacillin A 05: [Aib]5 → [Vxx]5 and [Aib]18 → [Glu]18 Krause et al., 2006b
Pept-B-XXXIVa 1937 1960 991.5 1149 789 39.59 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Ala Gly Aib Aib Pro Vxx Aib Vxx Glu Gln Pheol New: Pept-1966-d: [Lxx]12 → [Aib]12 Tamandegani et al., 2016
Pept-B-XXXIVb 1951 1974 998.5 1163 789 39.13 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Glu Gln Pheol New: (Positional isomer of Pept-B-XXXI and LII) → Pept-B-XXXI
Pept-B-XXXVa 1936 1959 991 1163 774 39.17 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol (Positional isomer of Pept-B-XX, XXXIIIa, XLIIb, XLVIa, and LVIII) → Pept-B-XX
Pept-B-XXXVb 1936 1959 991 1163 774 39.78 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Aib Gly Vxx Aib Pro Vxx Aib Aib Gln Gln Pheol (Positional isomer of Pept-B-XXXIIIb) → Pept-B-XXXIIIb
Pept-B-XXXVI 1964 1987 1005 1191 774 39.85 Ac Aib Ala Vxx Ala Vxx Aib Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol New: Paracelsin D: [Aib]3 → [Vxx]3 and [Aib]5 → [Vxx]5 Pócsfalvi et al., 1997
New: Saturnisporin SA III: [Aib]3 → [Vxx]3 and [Aib]5 → [Vxx]5 Rebuffat et al., 1993
New: Suzukacillin A 05: [Aib]3 → [Vxx]3 and [Aib]5 → [Vxx]5 Krause et al., 2006b
Pept-B-XXXVII 1937 1960 991.5 1163 775 40.54 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Glu Gln Pheol New: (Positional isomer of Pept-B-XXV) → Pept-B-XXV
Pept-B-XXXVIII 1950 1973 998 1163 788 40.13 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol (Positional isomer of Pept-B-XXIV, XXVII, XXXIIa, and XLVa) → Pept-B-XXIV
Pept-B-XXXIX 1964 1987 1005 1177 788 39.57 Ac Aib Ala Aib Ala Vxx Aib Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol New: Saturnisporin SA IV: [Aib]5 → [Vxx]5 Rebuffat et al., 1993
New: Suzukacillin A 07: [Aib]5 → [Vxx]5 Krause et al., 2006b
Pept-B-XL 1950 1973 998 1163 788 40.55 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Aib Gly Vxx Aib Pro Vxx Aib Vxx Gln Gln Pheol New: Trichoderma citrinoviride sequence 5: [Aib]6 → [Ala]6 (Positional isomer of Pept-B-XLVIIIa) Maddau et al., 2009
New: Trichoderma citrinoviride sequence 6: [Aib]6 → [Ala]6 Maddau et al., 2009
New: Trichoderma citrinoviride sequence 8: [Aib]6 → [Ala]6 Maddau et al., 2009
Pept-B-XLIa 1964 1987 1005 1177 788 40.98 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Vxx Aib Pro Vxx Aib Vxx Gln Gln Pheol Trichoderma citrinoviride sequence 5 (Positional isomer of Pept-B-XLVIIIb, LV, LVI, LXb, and LXI) Maddau et al., 2009
Trichoderma citrinoviride sequence 6 Maddau et al., 2009
Trichoderma citrinoviride sequence 8 Maddau et al., 2009
Pept-B-XLIb 1964 1987 1005 1177 788 40.82 Ac Aib Ala Aib Ala Aib Vxx Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol New: (Positional isomer of Pept-B-XXXIIb) → Pept-B-XXXIIb
Pept-B-XLIIa 1950 1973 998 1177 774 41.54 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Vxx Aib Pro Vxx Aib Aib Gln Gln Pheol (Positional isomer of Pept-B-XXX, XXXIIIc, XLVIb, and LIII) → Pept-B-XXX
Pept-B-XLIIb 1936 1959 991 1163 774 41.64 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol Paracelsin D (Positional isomer of Pept-B-XX, XXXIIIa, XXXVa, XLVIa, and LVIII) → Pept-B-XX
Pept-B-XLIII 1964 1987 1005 1191 774 41.26 Ac Aib Ala Aib Ala Vxx Vxx Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol New: Paracelsin B: [Aib]5 → [Vxx]5 and [Ala]6 → [Vxx]6 Pócsfalvi et al., 1997
New: Paracelsin D: [Aib]5 → [Vxx]5 and [Aib]6 → [Vxx]6 Pócsfalvi et al., 1997
New: Saturnisporin SA I: [Aib]5 → [Vxx]5 and [Ala]6 → [Vxx]6 Rebuffat et al., 1993
New: Saturnisporin SA III: [Aib]5 → [Vxx]5 and [Aib]6 → [Vxx]6 Rebuffat et al., 1993
New: Suzukacillin A 02, A 06: [Aib]5 → [Vxx]5 and [Ala]6 → [Vxx]6 Krause et al., 2006b
New: Suzukacillin A 05: [Aib]5 → [Vxx]5 and [Aib]6 → [Vxx]6 Krause et al., 2006b
New: Trichocellin TC-A-I, TC-A-III: [Aib]5 → [Vxx]5 and [Ala]6 → [Vxx]6 Wada et al., 1994
Pept-B-XLIV 1965 1988 1005.5 1191 775 41.65 Ac Aib Ala Aib Ala Vxx Vxx Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Glu Gln Pheol New: Trichocellin TC-B-I: [Aib]5 → [Vxx]5 and [Ala]6 → [Vxx]6 (Positionar isomer of Pept-B-L) Wada et al., 1994
Pept-B-XLVa 1950 1973 998 1163 788 41.92 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol (Positional isomer of Pept-B-XXIV, XXVII, XXXIIa and XXXVIII) → Pept-B-XXIV
Pept-B-XLVb 1978 2001 1012 1191 788 42.47 Ac Aib Ala Vxx Ala Aib Vxx Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol New: Paracelsin H: [Aib]3 → [Vxx]3 and [Ala]5 → [Vxx]5 (Positional isomer of Pept-B-XLIX) Pócsfalvi et al., 1997
New: Saturnisporin SA II: [Aib]3 → [Vxx]3 and [Ala]6 → [Vxx]6 Rebuffat et al., 1993
New: Saturnisporin SA IV: [Aib]3 → [Vxx]3 and [Aib]6 → [Vxx]6 Rebuffat et al., 1993
New: Suzukacillin A 04, 08: [Aib]3 → [Vxx]3 and [Ala]6 → [Vxx]6 Krause et al., 2006b
New: Suzukacillin A 07: [Aib]3 → [Vxx]3 and [Aib]6 → [Vxx]6 Krause et al., 2006b
New: Trichocellin TC-A-II, A-IV: [Aib]3 → [Vxx]3 and [Ala]6 → [Vxx]6 Wada et al., 1994
Pept-B-XLVIa 1936 1959 991 1163 774 42.42 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol (Positional isomer of Pept-B-XX, XXXIIIa, XXXVa, XLIIb, and LVIII) → Pept-B-XX
Pept-B-XLVIb 1950 1973 998 1177 774 42.42 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Vxx Aib Pro Vxx Aib Aib Gln Gln Pheol (Positional isomer of Pept-B-XXX, XXXIIIc, XLIIa, and LIII) → Pept-B-XXX
Pept-B-XLVII 1965 1988 1005.5 1177 789 42.13 Ac Aib Ala Aib Ala Vxx Aib Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Glu Gln Pheol New: Trichocellin TC-B-II: [Aib]5 → [Vxx]5 and [Gln]18 → [Glu]18 Wada et al., 1994
New: Saturnisporin SA IV: [Aib]5 → [Vxx]5 and [Gln]18 → [Glu]18 Rebuffat et al., 1993
New: Suzukacillin A 07: [Aib]5 → [Vxx]5 and [Gln]18 → [Glu]18 Krause et al., 2006b
Pept-B-XLVIIIa 1950 1973 998 1163 788 43.00 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Aib Gly Vxx Aib Pro Vxx Aib Vxx Gln Gln Pheol New: (Positional isomer of Pept-B-XL) → Pept-B-XL
Pept-B-XLVIIIb 1964 1987 1005 1177 788 42.85 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Vxx Aib Pro Vxx Aib Vxx Gln Gln Pheol (Positional isomer of Pept-B-XLIa, LV, LVI, LXb, and LXI) → Pept-B-XLIa
Pept-B-XLIX 1978 2001 1012 1191 788 42.56 Ac Aib Ala Vxx Ala Aib Vxx Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol New: (Positional isomer of Pept-B-XLVb) → Pept-B-XLVb
Pept-B-L 1965 1988 1005.5 1191 775 42.67 Ac Aib Ala Aib Ala Vxx Vxx Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Glu Gln Pheol New: (Positionar isomer of Pept-B-XLIV) → Pept-B-XLIV
Pept-B-LI 1978 2001 1012 1205 774 43.24 Ac Aib Ala Vxx Ala Vxx Vxx Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol New: Paracelsin B: [Aib]3 → [Vxx]3, [Aib]5 → [Vxx]5, and [Ala]6 → [Vxx]6 Pócsfalvi et al., 1997
New: Paracelsin D: [Aib]3 → [Vxx]3, [Aib]5 → [Vxx]5, and [Aib]6 → [Vxx]6 Pócsfalvi et al., 1997
New: Saturnisporin SA I: [Aib]3 → [Vxx]3, [Aib]5 → [Vxx]5, and [Ala]6 → [Vxx]6 Rebuffat et al., 1993
New: Saturnisporin SA III: [Aib]3 → [Vxx]3, [Aib]5 → [Vxx]5, and [Aib]6 → [Vxx]6 Rebuffat et al., 1993
New: Suzukacillin A 02, A 06: [Aib]3 → [Vxx]3, [Aib]5 → [Vxx]5, and [Ala]6 → [Vxx]6 Krause et al., 2006b
New: Suzukacillin A 05: [Aib]3 → [Vxx]3, [Aib]5 → [Vxx]5, and [Aib]6 → [Vxx]6 Krause et al., 2006b
New: Trichocellin TC-A-I, TC-A-III: [Aib]3 → [Vxx]3, [Aib]5 → [Vxx]5, and [Ala]6 → [Vxx]6 Wada et al., 1994
Pept-B-LII 1951 1974 998.5 1163 789 43.38 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Glu Gln Pheol New: (Positional isomer of Pept-B-XXXI and XXXIVb) → Pept-B-XXXI
Pept-B-LIII 1950 1973 998 1177 774 43.99 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Vxx Aib Pro Vxx Aib Aib Gln Gln Pheol (Positional isomer of Pept-B-XXX, XXXIIIc, XLIIa, and XLVIb) → Pept-B-XXX
Pept-B-LIV 1978 2001 1012 1191 788 44.00 Ac Aib Ala Aib Ala Vxx Vxx Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol New: Paracelsin H: [Aib]5 → [Vxx]5 and [Ala]6 → [Vxx]6 Pócsfalvi et al., 1997
New: Saturnisporin SA II: [Aib]5 → [Vxx]5 and [Aib]6 → [Vxx]6 Rebuffat et al., 1993
New: Saturnisporin SA IV: [Aib]5 → [Vxx]5 and [Ala]6 → [Vxx]6 Rebuffat et al., 1993
New: Suzukacillin A 04, 08: [Aib]5 → [Vxx]5 and [Ala]6 → [Vxx]6 Krause et al., 2006b
New: Suzukacillin A 07: [Aib]5 → [Vxx]5 and [Aib]6 → [Vxx]6 Krause et al., 2006b
New: Trichocellin TC-A-II, A-IV:[Aib]5 → [Vxx]5 and [Ala]6 → [Vxx]6 Wada et al., 1994
Pept-B-LV 1964 1987 1005 1177 788 44.44 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Vxx Aib Pro Vxx Aib Vxx Gln Gln Pheol (Positional isomer of Pept-B-XLIa, XLVIIIb, LVI, LXb, and LXI) → Pept-B-XLIa
Pept-B-LVI 1950 1973 998 1163 788 45.07 Ac Aib Ala Aib Ala Aib Ala Gln Aib Lxx Aib Gly Vxx Aib Pro Vxx Aib Vxx Gln Gln Pheol (Positional isomer of Pept-B-XLIa, XLVIIIb, LV, LXb, and LXI) → Pept-B-XLIa
Pept-B-LVII 1979 2002 1012.5 1191 789 45.36 Ac Aib Ala Aib Ala Vxx Vxx Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Glu Gln Pheol New: Trichocellin TC-B-II: [Aib]5 → [Vxx]5 and [Ala]6 → [Vxx]6 Wada et al., 1994
Pept-B-LVIII 1936 1959 991 1163 774 45.81 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol (Positional isomer of Pept-B-XX, XXXIIIa, XXVc, XLIIb, and XLVIa) → Pept-B-XX
Pept-B-LIX 1992 2015 1019 1205 788 45.74 Ac Aib Ala Vxx Ala Vxx Vxx Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol New: Paracelsin H: [Aib]3 → [Vxx]3, [Aib]5 → [Vxx]5, and [Ala]6 → [Vxx]6 Pócsfalvi et al., 1997
New: Saturnisporin SA II: [Aib]3 → [Vxx]3, [Aib]5 → [Vxx]5, and [Ala]6 → [Vxx]6 Rebuffat et al., 1993
New: Saturnisporin SA IV: [Aib]3 → [Vxx]3, [Aib]5 → [Vxx]5, and [Aib]6 → [Vxx]6 Rebuffat et al., 1993
New: Suzukacillin A 04, 08: [Aib]3 → [Vxx]3, [Aib]5 → [Vxx]5, and [Ala]6 → [Vxx]6 Krause et al., 2006b
New: Suzukacillin A 07: [Aib]3 → [Vxx]3, [Aib]5 → [Vxx]5, and [Aib]6 → [Vxx]6 Krause et al., 2006b
New: Trichocellin TC-A-II, A-IV: [Aib]3 → [Vxx]3, [Aib]5 → [Vxx]5, and [Ala]6 → [Vxx]6 Wada et al., 1994
Pept-B-LXa 1964 1987 1005 1177 788 46.50 Ac Aib Ala Aib Ala Aib Aib Gln Aib Vxx Aib Gly Lxx Aib Pro Vxx Aib Vxx Gln Gln Pheol (Positional isomer of Pept-B-XLIXa) → Pept-B-XLIXa
Pept-B-LXb 1964 1987 1005 1177 788 46.29 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Vxx Aib Pro Vxx Aib Vxx Gln Gln Pheol (Positional isomer of Pept-B-XLIa, XLVIIIb, LV, LVI, and LXI) → Pept-B-XLIa
Pept-B-LXI 1964 1987 1005 1177 788 48.35 Ac Aib Ala Aib Ala Aib Aib Gln Aib Lxx Aib Gly Vxx Aib Pro Vxx Aib Vxx Gln Gln Pheol (Positional isomer of Pept-B-XLIa, XLVIIIb, LV, LVIII, and LXb) → Pept-B-XLIa
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Variable residues are UNDERLINED in the table header; minor sequence variants are UNDERLINED in the sequences. Amino acid exchanges in new compounds are set in italic.

Table 4.

Sequences of the newly identified brevicelsins (group C) from Trichoderma species of the Longibrachiatum Clade and their similarities to known peptaibols available in the “Comprehensive Peptaibiotics Database.”

Peptide M [M+Na]+ [M+2Na]2+ b13 y7 rt-GK (min) R R1 R2 R3 R4 R5 R6 R7 R8 R9 R10 R11 R12 R13 R14 R15 R16 R17 R18 R19 R20 Compound identical or positionally isomeric with References
Brevicelsin-I 1851 1874 948.5 1078 774 28.72 Ac Aib Ala Aib Ala Aib - Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol New: Hypophellin 18, 35, 39: [Lxx]11 → [Aib]11 Röhrich et al., 2013
New: Paracelsin B, D: [Ala]6, [Aib]6 Pócsfalvi et al., 1997
New: Saturnisporin SA I;, SA III: [Ala]6, [Aib]6 Rebuffat et al., 1993
New: Suzukacillin A 02, A 06, A 05: [Ala]6, [Aib]6 Krause et al., 2006b
New: Trichocellin TC-A-I, -III: [Ala]6 Wada et al., 1994
Brevicelsin-II 1865 1888 955.5 1092 774 29.98 Ac Aib Ala Vxx Ala Aib - Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol New: HPV-9,−12,−20b: [Ala]3 → [Vxx]3, [Lxx]11 → [Aib]11 Röhrich et al., 2012
New: HPV-3: [Ser]3 → [Vxx]3, [Lxx]11 → [Aib]11 Röhrich et al., 2012
New: HPV-5: [Ala]3 → [Vxx]3, [Vxx]11 → [Aib]11 Röhrich et al., 2012
New: Hypophellin 17, 34: [Ala]3 → [Vxx]3, [Lxx]11 → [Aib]11 Röhrich et al., 2013
New: Hypophellin 20, 40: [Aib]3 → [Vxx]3, [Lxx]11 → [Aib]11 Röhrich et al., 2013
New: Hypophellin 18, 35, 39: [Aib]3 → [Vxx]3, [Lxx]11 → [Aib]11 Röhrich et al., 2013
New: Hypophellin 31: [Ser]3 → [Vxx]3, [Lxx]11 → [Aib]11 Röhrich et al., 2013
New: Paracelsin B, D: [Aib]3 → [Vxx]3 [Ala]6, [Aib]6, Pócsfalvi et al., 1997
New: Saturnisporin SA I; SA III: [Aib]3 → [Vxx]3, [Ala]6, [Aib]6 Rebuffat et al., 1993
New: Suzukacillin A 02, A 06, A 05: [Aib]3 → [Vxx]3, [Ala]6, [Aib]6 Krause et al., 2006b
New: Trichocellin TC-A-I, -III: [Aib]3 → [Vxx]3, [Ala]6 Wada et al., 1994
Brevicelsin-III 1852 1875 949 1078 775 30.56 Ac Aib Ala Aib Ala Aib - Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Glu Gln Pheol New: Hypophellin 38: [Lxx]11 → [Aib]11 Röhrich et al., 2013
New: Trichocellin TC-A-I, -III: [Ala]6, [Aib]6 Wada et al., 1994
Brevicelsin-IV 1865 1888 955.5 1078 788 31.82 Ac Aib Ala Aib Ala Aib - Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol New: Hypophellin 20, 40: [Lxx]11 → [Aib]11 Röhrich et al., 2013
New: Paracelsin H: [Ala]6 Pócsfalvi et al., 1997
New: Saturnisporin SA II, IV: [Ala]6, [Aib]6 Rebuffat et al., 1993
New: Suzukacillin A 04, A 08, 07: [Ala]6, [Aib]6 Krause et al., 2006b
New: Trichocellin TC-A-II, -IV: [Ala]6 Wada et al., 1994
Brevicelsin-V 1879 1902 962.5 1092 788 32.82 Ac Aib Ala Aib Ala Vxx - Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol New: Hypophellin 22, 45: [Lxx]11 → [Aib]11 (Positional isomer of Brevicelsin VIII) Röhrich et al., 2013
New: Paracelsin H: [Aib]5 → [Vxx]5, [Ala]6 Pócsfalvi et al., 1997
New: Saturnisporin SA II, IV: [Aib]5 → [Vxx]5, [Ala]6, [Aib]6 Rebuffat et al., 1993
New: Suzukacillin A 04, A 08, 07: [Aib]5 → [Vxx]5, [Ala]6, [Aib]6 Krause et al., 2006b
New: Trichocellin TC-A-II, -IV: [Aib]5 → [Vxx]5, [Ala]6 Wada et al., 1994
Brevicelsin-VI 1865 1888 955.5 1092 774 33.20 Ac Aib Ala Aib Ala Vxx - Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Aib Gln Gln Pheol New: Hypophellin 4: [Lxx]11 → [Aib]11 Röhrich et al., 2013
New: Paracelsin B, D: [Aib]5 → [Vxx]5, [Ala]6, [Aib]6, Pócsfalvi et al., 1997
New: Saturnisporin SA I;, SA III: [Aib]5 → [Vxx]5, [Ala]6, [Aib]6 Rebuffat et al., 1993
New: Suzukacillin A 02, A 06, A 05: [Aib]5 → [Vxx]5, [Ala]6, [Aib]6 Krause et al., 2006b
New: Trichocellin TC-A-I, -III: [Aib]5 → [Vxx]5, [Ala]6 Wada et al., 1994
Brevicelsin-VII 1866 1889 956 1078 789 33.65 Ac Aib Ala Aib Ala Aib - Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Glu Gln Pheol New: Hypophellin 21, 43: [Lxx]11 → [Aib]11 Röhrich et al., 2013
New: Hypocitrinin-7: [Lxx]11 → [Aib]11 Röhrich et al., 2014
Brevicelsin-VIII 1879 1902 962.5 1092 788 36.19 Ac Aib Ala Aib Ala Vxx - Gln Aib Lxx Aib Gly Aib Aib Pro Vxx Aib Vxx Gln Gln Pheol New: (Positional isomer of Brevicelsin V) → Brevicelsin V
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Variable residues are UNDERLINED in the table header; minor sequence variants are UNDERLINED in the sequences. Amino acid exchanges in new compounds are set in italic.

Of the 49 sequences from group A consisting exclusively of 20-residue peptaibols, 27 have been previously described in the literature, and 22 were new, differing by 1–3 amino acids from known sequences (Table 2). Group B also comprises 20-residue sequences (Table 3). The main difference between group B and group A peptaibols is located at the R12 position, where Aib instead of Lxx is present in most of the group B sequences. Another major difference from group A is that the R5 position is not conserved due to a high percentage of Vxx instead of Aib. Of the 86 group B sequences, 37 were identified as new. An entirely new compound, Pept-B-LIX, with a mass of 1992 Da was detected in the crude extracts of three strains (T. konilangbra SzMC 22607, T. flagellatum SzMC 22608 and T. sinensis SzMC 22609). All sequences of group C produced by three strains (T. flagellatum SzMC 22608, T. sinensis SzMC 22609 and T. parareesei SzMC 22615) proved to belong to a new group of peptaibols, which was named brevicelsins, as they are similar to, but one amino acid shorter than paracelsins (Brückner and Graf, 1983; Pócsfalvi et al., 1997) (Table 4).

Qualitative and Semi-quantitative Peptaibol Profiles of the Strains

After investigation of all strains producing peptaibols from group A, “a” and “b” versions of their peptaibol compounds were apparent. Pept-A-XI has a “c” version of the compound, and a few others are represented by only a single sequence (Supplementary Table 7). Compounds such as Pept-A-IV-a and -b were produced constantly in high quantities by all strains. Both Pept-A-IX-a and -b were produced in high quantities by all strains except T. aethiopicum SzMC 22602, T. pinnatum SzMC 22603 and T. longibrachiatum SzMC 1775. Similarly, Pept-A-XVI-a and -b were produced by all strains. In this group, seven mainly produced peptaibol varieties appeared on the spectra, Pept-A-IV-a and -b, Pept-A-VI-a and -b, Pept-A-IX-a and -b, Pept-A-XV-a and -b, Pept-A-XVI-a and -b, Pept-A-XIXa as well as Pept-A-XXI-a and -b.

The analysis of the four T. longibrachiatum strains (SzMC 1773, 1775, 1776, and 12546) revealed similar, but still different, profiles (Supplementary Table 8). Environmental isolates of T. longibrachiatum produced more similar profiles, whereas the peptaibol profile of the clinical isolate was different from those of the three environmental strains. Pept-B-XX and Pept-B-XXVII were produced by all of the strains examined, whereas the other compounds were produced only by certain strains. Five peptaibol compounds (Pept-B-VII, Pept-B-XVII, Pept-B-XX, Pept-B-XXVII, and Pept-XLV-a and b) were produced at high levels. Certain strains could also produce other compounds, such as Pept-B-XXVIII, Pept-B-XXIX-a and b, Pept-B-XXXIIIa, Pept-A-IVb, Pept-XLIb, Pept-XLIII, Pept-B-XLVa, Pept-B-LI, Pept-B-LIV, and Pept-B-LVIb, at high levels. The most diverse peptaibol profile was observed in T. reesei QM6a (SzMC 22614), which produced 41 different peptaibol compounds, whereas the least diverse profiles were that of T. effusum SzMC 22611 and T. konilangbra SzMC 22607, which produced 11 and 12 sequences, respectively. Some species producing mostly group B peptaibols, T. reesei QM6A (SzMC 22614), T. saturnisporum SzMC 22606 and T. konilangbra SzMC 22607 could also produce peptaibols from group A. Interestingly, group A sequences could not be detected from the two mutant strains of T. reesei SzMC 22614 (T. reesei SzMC 22616 and SzMC 22617). Brevicelsins from group C were only produced by three species, T. sinensis, T. flagellatum and, to a lesser extent, T. parareesei. Brevicelsin I and Brevicelsin IV were produced by the examined strains (T. flagellatum SzMC 22608, T. sinensis SzMC 22609 and T. parareesei SzMC 22615) of all three species, but T. parareesei produced only these two compounds of group C in addition to the group B sequences.

We carried out a cluster analysis of the peptaibol diversity profiles in different Trichoderma species of the Longibrachiatum Clade based on the production levels of different peptaibols by various fungal producers (Supplementary Tables 7, 8). According to their peptaibol profiles, members of the Longibrachiatum Clade were divided into two main clusters (Figure 1). The first cluster involves species producing exclusively group A peptaibols. Among them, T. novae-zelandiae is characterized with a relatively poor, but sharply distinct, profile of abundantly produced peptaibol compounds from group A, like Pept-A-XXIb, XVIb, XII, Vb, Ib, and IIIc. Further species in this cluster include members of the phylogenetic subclades Longibrachiatum/Orientale and Citrinoviride/Pseudokoningii, along with the lone lineages T. ghanense and T. capillare (Table 1). This cluster is consisting of three subclusters, the first one containing the closely related species T. aethiopicum and T. pinnatum and the second one involving T. longibrachiatum and T. orientale—all belonging to the phylogenetic subclade Longibrachiatum/Orientale—while the third subcluster is corresponding with the subclade Citrinoviride/Pseudokoningii (Table 1; Figure 1). The second main cluster is comprised of species producing mainly group B peptaibols and includes 2 subclusters, with the first containing the phylogenetic subclades Parareesei/Reesei, Saturnisporum and the lone lineages T. andinense and T. effusum, while the second harboring the three examined species from subclade Konilangbra/Sinensis (Table 1; Figure 1). All three examined members of this subclade produced the entirely new compound Pept-B-LIX (1992 Da).

Figure 1.

Figure 1

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Heatmap showing the correlation between the production of peptaibols and the phylogenetic relationship between the strains. Monophyletic species are indicated by the bottom bar of the same color, species attributed to single phylogenetic lineages are marked with a star, while mutant strains are indicated with a filled pentagon. The color scale denotes production level increasing from zero (deep blue) to high (deep red).

Annotation of NRPS Domains From the Genomes of T. longibrachiatum, T. citrinoviride, T. reesei, and T. parareesei

The NRPS gene sequences from T. longibrachiatum (https://genome.jgi.doe.gov/Trilo1/Trilo1.home.html, Xie et al., 2014), T. citrinoviride (https://genome.jgi.doe.gov/Trici4/Trici4.home.html), T. reesei (https://genome.jgi.doe.gov/Trire2/Trire2.home.html, Martinez et al., 2008) and T. parareesei (NCBI Bioproject Id: PRJNA287603, Yang et al., 2015) predicted by the SMIPS software were analyzed using the fungiSMASH software pipeline (Blin et al., 2017), which was designed to identify gene clusters of secondary metabolite biosynthesis from nucleotide sequences and to predict the products of the clusters identified. The T. longibrachiatum, T. citrinoviride, T. reesei, and T. parareesei genome sequences contain genes encoding 20-module NRPSs of 69.505, 68.508, 69.516, and 69.516 bp, as well as 14-module NRPSs of 43.422, 44.196, 49.386, and 52.395 bp with adenylation, condensation, thiolation, single acyl transferase and thioesterase domains. Figure 2 shows the schematic structure of the 20-mer NRPS gene cluster and the encoded modular enzyme from T. longibrachiatum. The 5′ ends of 20-module synthetase sequences contain a ketide synthase, whereas a Phe-specific permease-like and an aldo/keto reductase-like gene can be found downstream from the NRPS gene cluster. These two genes were also identified in the region downstream of the 18-module peptaibol synthetase gene clusters of the mushroom green mold agents T. aggressivum and T. pleuroti (Marik et al., 2017a). The identification of the presence of Pro in the peptaibol sequences and the close proximity of a Pro-specific permease gene to the NRPS gene cluster in these six Trichoderma species suggests that the permease may have a role in the secretion of these secondary metabolites.

Figure 2.

Figure 2

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Schematic structure of the 20-residue non-ribosomal peptide synthase (NRPS) from T. longibrachiatum. (A) Up- and downstream regions of the gene encoding the NRPS enzyme; pink pentagons indicate genes of additional putative biosynthetic enzymes (cyt: cytochrome P450, phe: phenylalanine-specific permease, ald: aldo/keto reductase). (B) The 20 modules of the NRPS. AT, acyl transferase; C, condensation module; A, adenylation module; TD, thioesterase domain.

Table 5 shows the incorporated amino acids predicted by the NRPS/PKS substrate predictor and NRPSPredictor3, based on the annotated adenylation domains and the eight amino acid residue signature sequences. The four 20-module NRPSs from the Longibrachiatum Clade were identical in positions R15 and R16 according to the signature sequences and the incorporated amino acids, respectively. Two positions (R6 and R9) were different only in T. longibrachiatum, whereas position R17 showed identity between T. longibrachiatum/T. citrinoviride, and T. reesei/T. parareesei. The most variable position was predicted to be R12, in which all signature sequences differed, and the incorporated amino acid was different in the case of T. citrinoviride.

Table 5.

Comparison of signature sequences of NRPS modules and predicted amino acid incorporations with the detected amino acid composition of the 20-residue peptaibols in the case of four Trichoderma species from the Longibrachiatum Clade.

Trichoderma reesei Trichoderma citrinoviride Trichoderma longibrachiatum Trichoderma parareesei
Amino acid position in peptaibols Sequence of amino acid binding pocket in NRPS module Possible amino acids predicted NRPS/PKS substrate predictor/ NRPSPredictor3 SVM Amino acids detected in peptaibol sequences Sequence of amino acid binding pocket in NRPS module Possible amino acids predicted NRPS/PKS substrate predictor /NRPSPredictor3 SVM Amino acids detected in peptaibol sequences Sequence of amino acid binding pocket in NRPS module Possible amino acids predicted NRPS /PKS substrate predictor /NRPSPredictor3 SVM Amino acids detected in peptaibol sequences Sequence of amino acid binding pocket in NRPS module Possible amino acids predicted NRPS /PKS substrate predictor/ NRPSPredictor3 SVM Amino acids detected in peptaibol sequences
1 D L G Y L A G V Aib, Iva/Iva Aib D L G Y L A G V Aib, Iva/Iva Aib D L G Y L A G V Aib, Iva/Iva Aib D L G Y L A G V Aib, Iva/Iva Aib
2 D I L F N G L I Ala/– Ala D I L F N G L I Ala/– Ala D I L F N G L I Ala/– Ala D I L F N G L I Ala/– Ala
3 D L G F L A G V Aib, Iva/Iva Aib, Ala D L G F L A G V Aib, Iva/Iva Aib D L G F L A G V Aib, Iva/Iva Aib, Iva D L G F L A G V Aib, Iva/Iva Aib
4 D V G F V A G V Aib, Iva/ala Ala D V G F V A G V Aib, Iva/ala Ala D V G F V A G V Aib, Iva/ala Ala D V G F V A G V Aib, Iva/ala Ala
5 D L G F L A G V Aib, Iva/Iva Aib D L G F L A G V Aib, Iva/Iva Aib D L G F L A G V Aib, Iva/Iva Aib D L G F L A G V Aib, Iva/Iva Aib
6 D V G C I E G V Aib, Iva/Iva Ala D V G C I E G V Aib, Iva/Iva Ala D V G C I A G V Aib, Iva/– Ala D V G C I E G V Aib, Iva/Iva Ala
7 D G G M V G G N Gln/Gln Gln D G G M V G G N Gln/Gln Gln D G G M V G G N Gln/Gln Gln D G G M V G G N Gln/Gln Gln
8 D L G Y L A G V Aib, Iva/Iva Aib D L G Y L A G V Aib, Iva/Iva Aib D L G Y L A G V Aib, Iva/Iva Aib D L G Y L A G V Aib, Iva/Iva Aib
9 D A F L I G G V Aib, Iva/Leu Iva, Ile D A F L I G G V Aib, Iva/Leu Iva, Ile D A F L L G A V Ala/Als Iva, Ile D A F L I G A V Aib, Iva/Leu Iva, Ile
10 D L G Y L A G V Aib, Iva/Iva Aib D L G Y L A G V Aib, Iva/Iva Aib D L G Y L A G V Aib, Iva/Iva Aib D L G Y L A G V Aib, Iva/Iva Aib
11 D V G Y L I A V Aib, Iva/– Gly D V G Y L I A V Aib, Iva/– Gly D V G Y L I A V Aib, Iva/– Gly D V G Y L I A V Aib, Iva/– Gly
12 D L G Y L A G - Aib, Iva/– Ile, Iva, Aib D L G Y L A G V Aib, Iva/ala Ile D F G F L G A V Aib, Iva/– Ile, Iva D L A Y L A G - Aib, Iva/– Ala, Iva, Aib
13 D L G F L A G V Aib, Iva/Iva Aib D L G F L A G V Aib, Iva/Iva Aib D L G F L A G V Aib, Iva/Iva Aib D L G Y L A G V Aib, Iva/Iva Aib
14 D V L F C G L I Pro/– Pro D V L F C G L I Pro/– Pro D V L F C G L I Pro/– Pro D V L F C G L I Pro/– Pro
15 D A G M I I G V Aib, Iva/Iva Iva D A G M I I G V Aib, Iva/Iva Iva D A G M I I G V Aib, Iva/Iva Iva D A G M I I G V Aib, Iva/Iva Iva
16 D L G F L A G V Aib, Iva/Iva Aib D L G F L A G V Aib, Iva/Iva Aib D L G F L A G V Aib, Iva/Iva Aib D L G F L A G V Aib, Iva/Iva Aib
17 D M G W F A G - Aib, Iva/Iva Aib, Iva D M G W F A G V Aib, Iva/Iva Aib, Iva D M G W F A G V Aib, Iva/Iva Aib, Iva D M G W F A G - Aib, Iva/Iva Aib, Iva
18 D G G M V G G N Gln/Gln Glu, Gln D G G M V G G N Gln/Gln Glu, Gln D G G M V G G N Gln/Gln Glu, Gln D G G M V G G N Gln/Gln Glu, Gln
19 D G G M V G G N Gln/Gln Gln D G G M V G G N Gln/Gln Gln D G G M V G G N Gln/Gln Gln D G G M I G G N Gln/Gln Gln
20 D A A F I M G V –/– Pheol D A A F I M G V –/– Pheol D A A F I M G V –/– Pheol D A A F I M G V –/– Pheol
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Comparison of the amino acids predicted by the NRPS/PKS substrate predictor and the ones detected showed agreement at 11 positions in all four species. In positions R6, R11 and R18, the prediction did not match with the detected Ala, Gly and Glu, respectively. Position R11 of the four species showed identity with position R10 of T. aggressivum (Marik et al., 2017a) in its signature sequence (DVGYLIAV), but the amino acid prediction in these positions was incorrect in all cases. At the last position, the predictor software identified the signature sequence of adenylation domains, but the amino acid prediction failed. These unsuccessful predictions suggest that these signature sequences are missing from the database. Based on the signature sequences, the highest variability is in position R12, where the amino acids detected are also variable.

Structural Characterization of 20- and 19-Residue Peptaibols

Two previously described sequences, Paracelsin B (AcAib-Ala- Aib-Ala-Aib-Ala-Gln-Aib-Leu-Aib-Gly-Aib-Aib-Pro-Val-Aib-Aib-Gln-Gln-Pheol) and Paracelsin H (AcAib-Ala-Aib-Ala-Aib-Ala-Gln-Aib-Leu-Aib-Gly-Aib-Aib-Pro-Val-Aib-Val-Gln-Gln-Pheol), together with their 19-residue counterparts Brevicelsin I (AcAib-Ala-Aib-Ala-Aib-Gln-Aib-Leu-Aib-Gly-Aib-Aib-Pro- Val-Aib-Aib-Gln-Gln-Pheol) and Brevicelsin IV (AcAib-Ala- Aib-Ala-Aib-Gln-Aib-Leu-Aib-Gly-Aib-Aib-Pro-Val-Aib-Val-Gln-Gln-Pheol) were selected for structural characterization. Based on their sequences, Paracelsin B and H appear to correspond with Pept-B-XII and Pept-B-XVIII, respectively, both of which were produced by six examined species (T. reesei, T. saturnisporum, T. andinense, T. effusum, T. parareesei, and T. flagellatum). Our aim was to observe structural differences resulting from the loss of Ala at the R6 position.

All peptides show a strong tendency to form right-handed helical structures with a slight bend at the Aib-Pro position (Figure 3). Cluster analysis of the simulation trajectories of all four peptaibols revealed different energetically stable conformations that occur during folding, and the representative structures of the most populated cluster are provided for each peptaibol. All peptides fold into an energetically favored, highly bent helical conformation along with a linear helical conformation. Based on the reweighted potential of mean force (PMF) values calculated for end-to-end distance (distance in Å from the N-terminus to the C-terminus), it can be speculated that a highly curved conformation for all peptaibols, except for Paracelsin H, lies in the energy minimum and requires an energy “jump” of <1 kcal mol−1 to attain the linear backbone conformation (Figure 4A). Overall, the end-to-end distance values as low as 5 to 27 Å, that lie close to the energy minima, show that all conformations starting from a hairpin-like helix structure to a straight backbone with just a slight bend are easily accessible. The PMF values increase rapidly beyond these two points for all four peptaibols, as shown in the inset image focusing only on PMF values up to 2 kcal mol−1. However, the sequences Paracelsin B and Brevicelsin I, with an Aib residue in position R17, have higher PMF values for higher end-to-end distance values; the energy cost for attaining linearity of the helical backbone is slightly higher than in Paracelsin H and Brevicelsin IV, where a Val residue replaces Aib in the R17 position. The energy minimum for Paracelsin H lies at an end-to-end distance of 22 Å, whereas Brevicelsin IV exhibits a slight fall at this point, even though its energy minimum also lies at 10 Å. The presence of Aib residue in position R17 (in Paracelsin B and Brevicelsin I) results in a highly dynamic folding process, which means that many conformations were visited during the trajectory, whereas Val in the same position (in Paracelsin H and Brevicelsin IV) led to fewer energetically stable conformers. The root-mean-square-atomic fluctuation (RMSF) graph (Figure 4B) shows higher fluctuation of N-terminus region for all peptides. No other significant differences were observed between the RMSF values of the 19-residue peptaibols, Brevicelsins I and IV, in comparison to 20-residue peptaibols, Paracelsins B and H, except that the sequences containing more Aib residues show a slight elevation in atomic fluctuation at the corresponding sequence position. For example, at R16 for Brevicelsin I and R17 for Paracelsin B, also, the R6 Aib in Paracelsins B and H shows higher average atomic fluctuation than the R6 Gln of Brevicelsins I and IV. This observation establishes the fluctuating and dynamic nature of the Aib residue in peptaibol sequences which can be explained by its tendency to oscillate between right- and left-handed helical forms. The Gln residues at R7 and R6 positions of paracelsins and brevicelsins, respectively, show a sharp dip in atomic fluctuation indicating higher stability in comparison to the C-terminal Gln residues and highlights importance of glutamines in ion-channel stabilization (Whitmore and Wallace, 2004).

Figure 3.

Figure 3

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Representative structures of the most populated clusters for each peptaibol simulation. The simulations were carried out for 1,000 ns in each case.

Figure 4.

Figure 4

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(A) The reweighted potential of mean force (PMF in kcal mol−1) values calculated for end-to-end distance (in Å). The 20-residue paracelsins have been plotted in black, whereas 19-residue brevicelsins are shown in red. The zoomed version of the plot is provided in the inset. The end-to-end values where PMF is zero or close to zero are the energetically stable values and define the most favorable linear conformation. (B) The root-mean-square deviation (in Å) calculated for each residue for all sequences gives an idea of the average fluctuation undergone by the system. Par, Paracelsin; Brev, Brevicelsin.

Antifungal Effects of T. reesei Peptaibols on Filamentous Fungi

The purified peptaibol extracts of T. reesei QM9414 were tested on human and plant pathogenic filamentous fungi, furthermore, the producer strain itself, as well as its Δlae1 mutant (Table 6). Treatment with 0.4 and 0.2 mg ml−1 purified peptaibol solution resulted in growth inhibition of all strains, whereas a weaker, but still notable, inhibition was detected after treatment with the purified extract at a concentration of 0.1 mg ml−1. The peptaibol extract from T. reesei QM9414 exhibited an inhibition profile highly similar to that of alamethicin.

Table 6.

Antifungal activity of the purified peptaibol extract from T. reesei QM9414 to filamentous fungi.

Tested filamentous fungal strain MIC of purified peptaibol extract (mg ml−1) MIC of alamethicin* standard (mg ml−1) MIC of nystatin standard (mg ml−1)
Alternaria alternata SzMC 16085 0.1 0.05 0.003125
Aspergillus fumigatus SzMC 23245 0.1 0.1 0.0125
Fusarium falciforme SzMC 11407 0.05 0.05 0.025
Fusarium keratoplasticum SzMC 11414 0.1 0.1 0.05
Fusarium solani SC SzMC 11467 0.1 0.1 0.05
Phoma cucurbitacearum SZMC 16088 0.05 0.05 0.1
Trichoderma reesei QM9414 0.1 0.05 0.00625
Trichoderma reesei QM9414 G2Δlae1 0.05 0.05 0.00625
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SC, species complex.

*

Harzianum A contamination could not be detected in the alamethicin standard based on the exact mass of its deprotonated molecular ion ([M-H]−, m/z = 399.1808).

Bioactivities of T. reesei Peptaibols on Arabidopsis thaliana Plants

In order to evaluate the value of peptaibols as antifungal agents for plant protection, the purified (98%) peptaibol extract of T. reesei QM9414 was investigated for toxicity in the model plant A. thaliana. The extract was diluted to 50, 10, 5, 1, 0.5, 0.3, 0.1, and 0.05 mg ml−1. All of the treated plants were inhibited after treatment with the peptaibol extract at concentrations of 50, 10, and 5 mg ml−1. Root growth was observed only at concentrations ≤ 1 mg ml−1; however, inhibited growth could be observed down to concentrations of 0.1 mg/ml (Figure 5). Treatment with 1 mg ml−1 peptaibol solution resulted in a hook formation of the primary roots. Chlorophyll-a, -b and carotenoid levels decreased after treatment with extracts of ≥0.3 mg ml−1 (Figure 6). Treatment with a peptaibol solution of 0.1 mg ml−1 resulted in a similar rate of production of photosynthetic pigments but an increased anthocyanin level in 15-day-old plants. The root growth of these plants was suppressed in 6- to 9-day-old plants, although the plants showed normal biomass and could probably eventually survive this minimal toxicity because of the increased levels of anthocyanin (Figure 7).

Figure 5.

Figure 5

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Primary root growth of 6 (A), 7 (B), 8 (C), and 9 (D) days old Arabidopsis thaliana plants after treatment with peptaibol extract from Trichoderma reesei QM9414. Methanol was used for the control plants as all peptaibol extracts were prepared in this solvent. Significance is assessed based on P-values: *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001 and ****P ≤ 0.0001.

Figure 6.

Figure 6

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Pigment content of 15-day-old Arabidopsis thaliana leaves after treatment with peptaibol extract from Trichoderma reesei QM9414: chlorophyll-a (A), chlorophyll-b (B), carotenoids (C) and anthocyanins (D). Methanol was used for the control plants. Significance is assessed based on P-values: *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001 and ****P ≤ 0.0001.

Figure 7.

Figure 7

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Biomass of 15-day-old Arabidopsis thaliana plants after treatment with peptaibol extract from Trichoderma reesei QM9414. Methanol was used for the control plants. Significance is assessed based on P-values: *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001 and ****P ≤ 0.0001.

Bioactivities of T. reesei Peptaibols on Mammalian Cells

The endpoint of toxic concentration—the last dilution step of the purified peptaibol solution which is toxic to mammalian cells—was determined for the peptaibol extract of T. reesei QM9414 (Table 7). After 20 min incubation at 37°C or 24 h at room temperature, the boar sperm motility inhibition end point was detected after treatment with 3 μg ml−1 peptaibol solution. The acrosome of the exposed sperm cells reacted at the same concentration, which inhibited motility, indicating that the toxic effect involves the plasma membrane. The inhibition end point of proliferation in porcine kidney PK-15 cells was observed at a concentration of 8 μg ml−1 peptaibol solution.

Table 7.

Toxicity of the peptaibol extract from T. reesei QM9414 to boar sperm and porcine kidney cells.

EC50 (μg ml−1)
Purified peptaibol extract Sperm motility inhibition Acrosome reaction Inhibition of proliferation of Porcine kidney cells PK-15
20 min 24 h
T. reesei QM9414 3 3 3 8
REFRENCE SUBSTANCE
Alamethicin 5 0.2 0.2 8
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The values are the median of three measurements, represented by four microscopic fields. The variation between measurements was one dilution step.

Discussion

In this study, the structural diversity and bioactivity of peptaibol compounds produced by Trichoderma species belonging to the Longibrachiatum Clade were investigated and compared. The Longibrachiatum Clade is ecologically highly versatile as it contains both environmental and opportunistically pathogenic species, some of which can be found worldwide, whereas others are ecologically restricted. In total, 143 20-residue peptaibols could be identified from the 17 species examined, including 59 new and 76 recurrent compounds, as well as eight new 19-residue sequences. The peptaibols can be categorized into groups A, B and C, based on their primary structure, where groups A and B consist of 20-residue peptaibols, whereas group C is comprised exclusively of 19-residue sequences. The main difference between peptaibols of group A in relation to group B is in the R12 position. Sequence analysis identified several conserved regions along with some variable positions (R3, R5, R6, R10, R12, and R17), which have also been reported in a previous study (Pócsfalvi et al., 1997). Vxx was usually found instead of Ala and Aib at certain variable positions like R3, R5 and R6, which has never been observed among similar peptaibols. Although all of these amino acids have helix-forming properties, a substitution by Val would render a more linear and less fluctuating helical conformation owing to its bulkier sidechain. The highly curved backbone conformation is not energetically favored with increasing number of Val in peptaibol sequences. It has been hypothesized that the equilibrium between the bent (closed form) and linear conformations (open amphipathic form) may act as a “conformational switch” of voltage gating in ion channels across bilayers (North et al., 1995). Clearly, such substitutions have an important functional relevance, especially at subterminal positions like R3 and R17.

Brevicelsins from group C form a new family of 19-residue peptaibols similar to, but one amino acid shorter than group B sequences. They are not N-terminally truncated derivatives of their full-length precursors—like it is the case for the 16-residue brevikindins deriving from 18-residue trichokindin-like peptaibols (Degenkolb et al., 2016) – but differ from group B sequences by the internal deletion of position 6. This position is critical, since the following Gln plays an important role in the formation of ion channels (Wilson et al., 2011). Brevicelsins could be found only in three species: T. flagellatum, T. sinense and T. parareesei. A full genome sequence is available for T. parareesei, analysis of this sequence, however, revealed no extra 19-module NRPS synthetases but only a 20-module enzyme. The 19-residue peptaibols could be produced by the same, 20-module NRPS via the interaction of non-neighboring modules known as internal module skipping. The mechanisms of this phenomenon resulting in additional classes of 10-, 13-, 18-, and 19-residue peptaibols were proposed by Degenkolb et al. (2012). R6 is also skipped in T. phellinicola peptaibols (Röhrich et al., 2013), which does, however, contain Lxx in position R12, similar to group A peptaibols and unlike brevicelsins with Aib in this position.

The unique group A peptaibol profile of T. novae-zelandiae (Figure 1) may be related to the geographical origin of this species, which is endemic to New Zealand, and to its occupying a basal position in the Longibrachiatum Clade (Samuels et al., 2012). This species has tuberculate conidia, a trait also found in the Viride Clade (Jaklitsch et al., 2006), and it may be an ancestral trait of the Longibrachiatum Clade (Druzhinina et al., 2012). Our results suggest that the production of group A peptaibols may be another ancestral trait of the Longibrachiatum Clade, while the switch to the production of group B peptaibols might have occurred multiple times and seems therefore to be the result of convergent evolution. This switch from group A to group B has not fully completed in certain species: wild-type T. reesei as well as T. saturnisporum and T. konilangbra are also producing some group A compounds in addition to group B peptaibols.

Except from T. reesei, which was separated from its closest relative T. parareesei, the clustering based on peptaibol profiles reflected the close relationships within phylogenetic subclades in most of the cases (e.g., within subclades Longibrachiatum/Orientale, Citrinoviride/Pseudokoningii, or Konilangbra/Sinensis). For example, the species from the Konilangbra/Sinensis subclade are phylogenetically close to each other and are only known from the Paleotropical/Asian areas including Ethiopia (T. flagellatum), Uganda (T. konilangbra) and Taiwan (T. sinensis) (Samuels et al., 2012). The very close relationship of T. sinensis and T. flagellatum is also reflected by their ability to produce group C peptaibols in addition to group B sequences. The phylogenetic relationships between the subclades are less reflected by the clustering based on peptaibol profiles. Distantly related subclades (e.g., Longibrachiatum/Orientale and Citrinoviride/Pseudokoningii) may share similar profiles, while closely related subclades may exhibit substantially different ones—e.g., members of subclade Citrinoviride/Pseudokoningii produce group A peptaibols, while group B compounds are produced by their close relative T. effusum. This could be explained by multiple events of switching from the production of group A to group B during the evolution of the Longibrachiatum Clade.

Based on molecular dynamics simulations, 20-residue peptaibols result in higher linearity of helices than their 19-residue counterparts and are also relatively stable in terms of the atomic fluctuations of each residue. Paracelsins B, H and their 19-residue deletion sequences Brevicelsin I and IV all fold into right-handed helical structures with a slight bend at the Aib-Pro bond, except for Brevicelsin IV where the bend occurs at the Aib11-Aib12 bond. The Aib-Pro bond at R13-R14 in the case of 20-residue sequences is important for the secondary structure of the bent molecule. An important observation was made with respect to Val substitution instead of Aib at R17 which seems to hinder the formation of a bent backbone in close proximity to the N-terminal side-chains, because it is a chiral, hydrophobic amino acid with a bulkier side-chain than that of the achiral Aib. Frequent occurrence of Aib could be detected at the termini of the sequences, which are very important for the determination of the formation of helical structures including α- or 310-helices (De Zotti et al., 2010; Gessmann et al., 2012a,b). The other promotor of the helical structure, D-Iva, is most often found close to the N-terminus, prior to the Gln-Aib bond in position R6, based on different previously described peptaibols such as boletusin 1, chrysospermins, peptaivirins, trichorzianins TA and TB, or the TA1938, 1924, 1910 and 1909a compounds (El-Hajji et al., 1987; Rebuffat et al., 1989; Dornberger et al., 1995; Lee et al., 1999; Yun et al., 2000; Panizel et al., 2013).

The growth of filamentous fungi pathogenic to plants or humans could be inhibited by the purified peptaibol extract of T. reesei QM9414. A stronger inhibition was observed in the case of the Δlae1 mutant of T. reesei than in the case of the other strains, suggesting that the mutation in the methyl transferase gene, which is known as a global epigenetic regulator of gene expression, may also affect tolerance to these metabolites. A previous study (Marik et al., 2018), in which crude peptaibol extracts were tested on several bacterial, yeast and filamentous fungal strains showed similar results. The inhibitory effects of peptaibols to bacteria and filamentous fungi have previously been reviewed (Szekeres et al., 2005; Daniel and Rodrigues Filho, 2007). It has also been demonstrated that purified trichokonin VI triggers a change of fungal membrane permeability and disintegration of subcellular structures, has an effect on mitochondrial membrane permeabilisation and intracellular ROS production, induces phosphatidylserine exposure and eventually triggers metacaspase-independent apoptosis in F. oxysporum (Shi et al., 2012).

Alamethicin, the most studied peptaibol was shown to induce resistance in plants (Leitgeb et al., 2007; Kredics et al., 2013), although it can also be toxic, causing lesions on Arabidopsis leaves (Rippa et al., 2010). At higher concentration, it induces rRNA cleavage-associated rapid death (Rippa et al., 2007). Alamethicin could permeabilise mainly the apical meristem and epidermis cells of the root tips, but not the basal meristem cells, cortex cells or the root cap of A. thaliana (Dotson et al., 2018). If the root was pretreated with cellulase, permeabilisation could not be observed. This study proved cellulose-induced resistance and cell-specific alamethicin permeabilisation of A. thaliana roots. Engelberth et al. (2001) successfully demonstrated the high biological activity of alamethicin that caused emission of volatile compounds from lima beans (Phaseolus lunatus) placed under low concentration of the peptaibol solution. When it was applied to Bryonia dioica tendrils at the same concentration, it elicited jasmonate-induced tendril coiling. Therefore, peptaibols may be used as potential elicitors of plant defense responses. Recently, antiviral activity of trichorzins was also reported on cowpea plants against Cucumber mosaic virus (Kai et al., 2018). In this recent study, bioactivity tests with the selected, purified peptaibol extract of T. reesei QM9414 demonstrated toxicity to A. thaliana plants at higher concentrations. An interesting effect of the peptaibol extract was the induction of hook formation in the root tips. A previous study revealed similar results, where the inoculation of A. thaliana with T. atroviride resulted in shortened primary root growth of the plants and ended in a hook formation, although the lateral root numbers were increased (Pelagio-Flores et al., 2017). An inhibitory effect on primary root growth in A. thaliana was also observed after interaction with T. longibrachiatum SMF2, and its peptaibols induced auxin production and disruption of the auxin response gradients in root tips (Shi et al., 2016).

Boar sperm cells are frequently used for the detection of toxins, which affect plasma membranes (Vicente-Carrillo, 2018; Castagnoli et al., 2018). Due to the high sensitivity of boar sperm cells to toxins, many studies have concluded that these tests are appropriate for toxin detection (Peltola et al., 2004; Andersson et al., 2009, 2010). Similar measurements of peptaibol extracts produced by T. longibrachiatum Thb have been reported, and a mixture of trilongins proved to be a stronger inhibitor of motility than trilongins alone, or any of the crude extracts (Mikkola et al., 2012). Single ion channels remained in an open state for a longer time when exposed to a combination of the long peptaibols (trilongins BI–BIV) with the short ones (trilongin AI), than for the long peptaibols alone. Furthermore, peptaibols (trichokonin VI) could inhibit HepG2 cancer cells by inducing autophagy and apoptosis through an influx of Ca2+, which triggered the activation of μ-calpain and proceeded to the translocation of Bax to mitochondria and the subsequent promotion of apoptosis (Shi et al., 2010). Another peptaibol, emericellipsin A, which is a short lipopeptaibol, exhibited selective cytotoxic activity against HepG2 and HeLa cell lines (Rogozhin et al., 2018), similar to culicinin D, another short linear peptaibol which has been described as a potent anticancer compound (He et al., 2006). In the present study, the partially purified peptaibol extract of T. reesei QM9414 proved to inhibit boar spermatozoa and porcine kidney PK-15 cells at 0.1 mg ml−1, which rises the question of a possible in vivo toxicity. Degenkolb et al. (2008) discussed this issue in detail and suggested that the toxicity of peptaibols may be well below the threshold of human consequence, and it may require direct contact with cell membranes, like in the case of common amphiphilic detergents. This is supported by previous observations demonstrating the very low toxicity of various peptaibols orally administered to rodents and ruminants (Hou et al., 1972; Nayar et al., 1973; Hino et al., 1994).

In conclusion, negative effects on Arabidopsis plants could not be detected below a certain concentration of the purified peptaibol extract from T. reesei QM9414, which could still inhibit plant pathogenic filamentous fungi. This observation suggests that purified peptaibol extracts may have potential value for plant protection. T. reesei is a well-characterized, widely used cellulase producer in the biotechnological industry, and so its peptaibols could be produced as the main product, or a valuable by-product of fermentation.

Data Availability

All datasets generated for this study are included in the manuscript and/or the Supplementary Files.

Author Contributions

LK, TM, AS, and CV designed the study and coordinated the draft of the manuscript. TM and DB took part in the extraction, HPLC separation, sequence determination, and antifungal activity testing of the peptaibol compounds. GE, DR, and AS conducted the mass spectrometry measurements. ID performed the sequence alignments and the comparative sequence analysis of peptaibol profiles. PU performed the annotation and bioinformatic analysis of NRPS gene clusters. CT contributed with the molecular dynamics simulations of peptaibols. TM, ÁS, and LB designed and performed the bioactivity tests on A. thaliana. MA and HS conducted the bioactivity assays on mammalian cells. TM, LK, and CV analyzed the results and designed the figures and tables. All authors read and approved the final manuscript.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

The authors wish to thank Prof. Cristian P. Kubicek and the Joint Genome Institute for the opportunity to use the genomes of T. longibrachiatum and T. citrinoviride for the bioinformatic analysis of NRPSs, Prof. Rainer Schuhmacher for kindly providing the offline version of the Peptaibiotics Database, as well as Dr. Ekaterina Shelest for her valuable suggestions regarding NRPS sequence mining.

Footnotes

Funding. This work was supported by the Hungarian Scientific Research Fund by grant K-116475, by the Széchenyi 2020 Programme (GINOP-2.3.2-15-2016-00052) as well as by the COST action 16110 (HuPlantControl). LK is grantee of the János Bolyai Research Scholarship (Hungarian Academy of Sciences) and the New National Excellence Programme (ÚNKP-18-4). Open access publication was supported by the University of Szeged.

Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2019.01434/full#supplementary-material

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Associated Data

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Supplementary Materials

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Data Availability Statement

All datasets generated for this study are included in the manuscript and/or the Supplementary Files.

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