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Effects of SeqA and IHF on ppGpp-induced block of DNA replication. Shown are representative flow cytometry profiles of the (A) wild-type or (B) ΔseqA, (C) ΔihfA, and (D) ΔihfB strains grown to an OD600 of ∼0.2 in LB. Pretreatment values are shown to the left, and results 90 min postinduction of RelA′ with 100 μM IPTG are shown to the right.
