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. 2018 Jan 2;10(41):4192–4204. doi: 10.18632/oncotarget.24115

Figure 2. MiR-873 inhibits CRC cell proliferation, migration and invasion in vitro.

Figure 2

(A) qRT-PCR analysis of miR-873 levels in LoVo and HCT116 stable infected cell lines. (B) MTT assay of LoVo-control and LoVo-miR-873 cells (left) and HCT116-control and HCT116-miR-873 cells (right). (C) Representative images and quantification of colonies formed by LoVo-control and LoVo-miR-873 cells (left) and HCT116-control and HCT116-miR-873 cells (right). (D) Western blotting analysis of p-ERK1/2, ERK1/2 and CyclinD1 in LoVo/HCT116-control and LoVo/HCT116-miR-873 cells. β-actin was used as a loading control. (E, F) Representative images and quantification of cell migration (E) and invasion (F) assays of LoVo/HCT116-control and LoVo/HCT116-miR-873 cells. (G) Western blotting analysis of EMT-related markers in LoVo/HCT116-control and LoVo/HCT116-miR-873 cells. β-actin was used as a loading control. Data (mean ± SEM) are representative of 3 technique replicates. Scale bars, 100 μm. *P < 0.05; **P < 0.01; ***P < 0.001.