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. Author manuscript; available in PMC: 2020 Jul 2.
Published in final edited form as: Structure. 2019 May 16;27(7):1156–1161.e4. doi: 10.1016/j.str.2019.04.005

Figure 2.

Figure 2.

Binding affinity between Nrd1 CID and Sen1 NIMs. (a). Fluorescence anisotropy binding data for wild-type NIM2 and the Y2058F mutant. The observed Kd values are indicated. (b). Fluorescence anisotropy competition binding data for wild-type NIM2 and three mutants. (c). Fluorescence anisotropy binding data for NIM2 with wild-type and mutant CID. (d). Fluorescence anisotropy competition binding data for NIM1, NIM2, NIM3 and the Pol II Ser5P CTD. Error bars represent standard deviations from triplicate measurements.