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. 2019 Jul 6;10:14. doi: 10.1186/s13227-019-0128-3

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Spatio-temporal expression of anterior lateral plate mesoderm (ALPM) marker tbx20 during zebrafish early development. A–C Whole-mount in situ hybridisation of zebrafish ALPM marker gene. Embryos were hybridised with tbx20 (Ai–vii, Bi–vii, Ci–vii) probes. tbx20 transcript can be first observed around bud stage in a reverse U-shaped domain (Aii). This domain covers the anterior lateral plate mesoderm. Transverse views (Ci–vii) were taken at the level shown by the dashed lines illustrated in lateral images (Bi–vii). Anterior is to the top in dorsal and lateral views. In all transverse views, dorsal side is oriented to the top. White arrowheads indicate the prospective heart primordium. 75E, 75% epiboly; hpf, hours post-fertilisation; ss, somite stage. Scale bar: 100 μm