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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Methods. 2019 Mar 26;162-163:42–53. doi: 10.1016/j.ymeth.2019.03.024

Figure 4.

Figure 4.

Kinetic stimulation and stabilization of mRNA binding to the translation preinitiation complex by eIF4A. The fraction of capped mRNA bound to the preinitiation complex was monitored by native gel shift at varying concentrations of eIF4A and plotted over time to derive the pseudo-first order apparent rate constant kapp. The kapp was plotted versus eIF4A concentration and fit to a hyperbolic function to determine the concentration of eIF4A that stimulates half-maximal rate, K1/2 or apparent affinity of eIF4A for the initiation complex.