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. 2019 Feb 20;15(7):1214–1233. doi: 10.1080/15548627.2019.1580103

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Figure 6. — Loss of VAPs affects PtdIns4P and the endosome pathway. (a) Immunofluorescence staining of PtdIns4P (red) in Vap33 mutant clones (non-green) of Drosophila fat body and salivary gland cells. (b) Immunofluorescence staining of PtdIns4P in control or VAPA/B siRNA-transfected HEK293T cells. (c) Immunofluorescence staining of Rab5 and Rab7 (red) in Vap33 mutant clones (non-green) of Drosophila salivary glands (d) Immunofluorescence staining of Rab7 (red) in Drosophila muscles of control and Vap33 mutant animals (e) Immunofluorescence staining of RAB5 in control or VAPA/B siRNA-transfected HEK293T cells. (f) Sgs3-GFP (green, a marker for secretory granules) in Vap33 mutant clones of Drosophila salivary gland. Wild-type nuclei are labeled with RFP.