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. 2019 Mar 1;15(8):1322–1332. doi: 10.1080/15548627.2019.1580511

Figure 3.

Figure 3.

Colocalization of autophagosomes and lysosomes. (a) Representative confocal z-sections of the optic tectum regions of 3-dpf larvae that underwent the indicated treatment for 24 h followed by 1 h of LysoTracker Red staining. Arrowheads indicate examples of puncta that are double positive for both eGFP-Map1lc3b and LysoTracker Red, i.e. autolysosomes. Scale bars: 10 μm. (b) Percentage of eGFP-Map1lc3-positive puncta that colocalize with LysoTracker Red-positive puncta; mean ± s.e.m. shown; n ≥ 40 for each group. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05, p > 0.05 (n.s.), one-way ANOVA with Tukey’s multiple comparison analysis (GraphPad Prism) .