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. 2019 May 15;212(3):711–728. doi: 10.1534/genetics.119.302262

Figure 6.

Figure 6

The FET4 locus is regulated by Paf1 and transcription of ncDNA upstream of the coding region. (A) Diagram of the FET4 locus and the position of a transcription termination sequence (HIS3 TTS) inserted 400 bp upstream of the FET4 start codon to block CUT 794/793 transcription. (B) Northern analysis of FET4 mRNA, CUT 794/793 and SCR1 RNA (loading control) from WT, paf1Δ, trf4Δ, and paf1Δ trf4Δ strains without the inserted TTS (KY2276, KY1702, KY2012, KY2016) or with the TTS (KY3466, KY2846, KY2851, KY2845). (C and D) Quantification of northern blot results for FET4 and CUT 794/793 normalized to SCR1. Error bars represent SEM and all statistically significant results are reported as asterisks that represent P values from Students t-test as in Figure 4. (E) Diagram of the observed effects of PAF1 and CUT794/793 at the FET4 locus.