Table 1.
Plasmids used for the generation of the GNrep polarity construct
| Plasmid | Name | Origin |
|---|---|---|
| 1 | pUC57‐2A | Generated in the lab by synthesis |
| 2 | pGTS‐mCherry | https://www.addgene.org/55052/ |
| 3 | pCAGGS‐G2 m | Gift from Holger Gerhardt Lab |
| 4 | pUC57‐GNrep | Generated in the lab |
| 5 | pME‐GNrep | Generated in the lab |
| 6 | pEF1‐GNrep | Generated in the lab |
| 7 | pROSA26‐DV2 | Nyabi et al. (2009) |
| 8 | p5E‐CAGGS‐MCS | Gift from Holger Gerhardt Lab |
| 9 | p5E‐CAGGS‐LSL | Generated in the lab |
| 10 | p3E‐pA‐FRT‐PGK.Neo‐pA‐FRT | Generated in the lab |
| 11 | pROSA26‐GNrep | Generated in the lab |
| 12 | pJFRC172‐10XUAS‐loxP‐dSTOP‐loxP‐myr::GFP | https://www.addgene.org/32145/ |
| 13 | pEF1‐DEST51 | ThermoFisher (12285011) |