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. 2019 Jul 8;6(4):ENEURO.0014-19.2019. doi: 10.1523/ENEURO.0014-19.2019

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Copyright © 2019 Hess et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 2. — The ALM neuron soma volume and the number and length of soma outgrowths are less effected by aging in long-lived C. elegans. A, Overview of quantified morphologic features in ALM neurons. Inset shows enlarged soma region with quantifications indicated (soma outgrowth count and length, blue; soma-volume, green). B, Soma outgrowth counts on days 1 and 8 of adulthood for WT and daf-2(e1370) mutants. Both conditions show a significant increase in soma outgrowth counts during aging (WT, p = 3.17e-15; daf-2(e1370), p = 1.94e-09). There is no significant difference between genotypes at day 1 (p = 0.376), but at day 8, soma outgrowth counts are significantly lower for daf-2(e1370) mutants compared to WT (p = 2.08e-03). For direct comparison of this data with previous studies, we replotted this data in percentage of ALM neurons with soma outgrowths (Extended Data Fig. 2-1). C, Lengths of individual soma outgrowths increase with age. This effect is statistically significant in WT animals (p = 2.20e-07), but no statistical significance is reached for daf-2(e1370) mutants (p = 0.149). At day 8 of adulthood, WT animals have significantly longer soma outgrowths (p = 3.38e-02). Error bars indicate bootstrapped 95% confidence interval of the mean, n is the number of outgrowth events scored. D, ALM soma volume decreases between day 1 and day 8 of adulthood (WT, p = 9.55e-12; daf-2(e1370), p = 1.52e-08), whereas WT somas are smaller than daf-2(e1370) somas (day 1, p = 3.60e-02; day 8, p = 2.44e-04). Error bars indicate bootstrapped 95% confidence interval of the mean, n is the number of neurons scored. E, F, Correlation of total soma outgrowth length (lengths of all soma outgrowths summed) versus soma volume of day 8 of adulthood C. elegans does not show a strong correlation for both WT (E, Pearson’s R = –0.127) and daf-2(e1370) mutants (F, Pearson’s R = –0.245). Data were collected in three independent trials and pooled for the analysis shown in this figure (B–F). ns = p > 0.05, * = p < 0.05, *** = p < 0.001, **** = p < 0.0001.Figure Contributions: Max Hess made all the figures.