Aw M, Armstrong TM, Nawata CM, Bodine SN, Oh JJ, Wei G, Evans KK, Shahidullah M, Rieg T, Pannabecker TL. Body mass-specific Na+-K+-ATPase activity in the medullary thick ascending limb: implications for species-dependent urine concentrating mechanisms. Am J Physiol Regul Integr Comp Physiol 314: R563–R573, 2018. First published January 3, 2018; doi:10.1152/ajpregu.00289.2017.—The values for the Fig. 5 y-axis were omitted from the final publication. The correct figure is shown below. The online version has been corrected.
Fig. 5.
Na+-K+-ATPase α1-protein expression in the ISOM of the kangaroo rat and the Sprague-Dawley rat, the latter with water ad libitum (control) or water restricted for 72 h (see methods). A: immunoblots with 10 μg of protein applied to each lane; each lane represents a different animal. Black lines denote where blot was cut for rearrangement of lanes. B: quantification of Na+-K+-ATPase α1-immunoblot normalized to β-actin; n = 5 (kangaroo rat) and n = 3 (Sprague-Dawley rats). Kangaroo rat and water-restricted Sprague-Dawley rat data are expressed relative to the mean of the control Sprague-Dawley rat, which was set to 1. Kangaroo rats were mixed gender. The statistical significance between sample means was determined with a one-way ANOVA and Tukey’s post hoc test. Mean values that share the same letter are not significantly different from each other.