Figure 7: Tau45-230 delayed neurite elongation in cultured hippocampal neurons.
(A & B) Tau45-230-transfected cultures were fixed and double-stained using GFP (A) and tubulin (B) antibodies. Most of GFP-tau45-230 (+) neurons (arrows) remained in stage 1 and 2, failing to elongate their axons after 2 days in culture. Scale bar: 20 μm. (C) Quantification of tau45-230 (−) and tau45-230 (+) neurons in stage 1, 2, and 3. Neurons in 30 fields from 3 independent culture preparations per experimental condition were counted. **Differs from untransfected controls, p < 0.01. ax: axon