(A) Experimentally determined sub-proteomes of M. xanthus 1622 by Venn diagram. The common proteins of all biological replicates were selected and compared with each other through the Venn diagram. There were 128 common proteins present in all three different combinations (boundary: ΔMXAN_0049-DK1622, non-boundary: DK1622-DK1622, and non-boundary: ΔMXAN_0049-ΔMXAN_0049) samples, while 373 unique proteins were observed when the boundary formed, and 72 and 45 unique proteins in the cases of both non-boundary samples (DK1622-DK1622 and ΔMXAN_0049-ΔMXAN_0049, respectively). (B) Ontological classification of the differentially regulated proteins within the boundary and the non-boundary and their comparison. The proteins were classified according to molecular function, cellular component, and biological process.