Skip to main content
. 2019 Jun 11;8(6):573. doi: 10.3390/cells8060573

Figure 3.

Figure 3

MiR-501-3p promotes C2C12 differentiation. We measured the expression of myogenic marker genes after C2C12 were transfected with miR-501-3p mimics or inhibitor. (A) MiR-501-3p mimics increased the percentage of Myosin-positive C2C12, and miR-501-3p inhibitor decreased the percentage of Myosin-positive C2C12. Representative images of the immunofluorescent staining for differentiated C2C12 are shown. Myosin: red, a molecular marker of myogenesis; DAPI: blue, cell nuclei; Merge: C2C12 fused into primary myotubes are shown in the insets. (B) The quantitative data of Myosin-positive C2C12 in Figure 3A. (C) The qRT-PCR confirmed miR-501-3p mimics increased (p < 0.05) mRNA level of myogenic marker genes after differentiation induction. (D) The qRT-PCR confirmed miR-501-3p inhibitor decreased (p < 0.05) mRNA level of myogenic marker genes after differentiation induction. (E) Western blot was used to measure the myogenic marker protein level after differentiation induction. ** p < 0.01; *** p < 0.001. The results are presented as mean ± S.E.M. of three replicates for each group. Magnification 100×. The scale bar on the photomicrographs represents 50 μm.