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. 2019 Jun 6;8(6):547. doi: 10.3390/cells8060547

Figure 2.

Figure 2

Glyoxalase 1 (Glo1) expression in anaplastic (ATC) and papillary (PTC) thyroid cancer samples. (A) Glo1 mRNA expression, evaluated by real-time PCR, after total RNA extraction from five ATC (#1–5) and five PTC samples (#6–10) and cDNA synthesis; (B) representative Western blot of Glo1 protein expression measured on lysates from five PTC (#6–10) and five ATC (#1–5) tissues. β-actin was used as loading control. The histogram, representing the densitometric analysis of the blots, indicates mean ± SD of all PTC (n = 5, #6–10) and ATC (n = 5, #1–5) samples analyzed; (C) representative immunohistochemical staining of Glo1 on PTC (n = 5, #16–20) and ATC (n = 5, #11–15) tissues (200× magnification); (D) Glo1 enzyme activity was measured in total protein extracts according to a spectrophotometric method, and increases in absorbance resulting from the formation of S-d-lactoylglutathione were monitored at 240 nm (see Materials and Methods). ** p < 0.01, *** p < 0.01.