Table 1.
Kinetic parameters of Na-K-ATPase transporters in IEC-18 cells.
| 0 Day | 1 Day | 2 Day | 3 Day | 4 Day | |
|---|---|---|---|---|---|
|
Vmax (picomole/mg protein·30 S) |
742.5 ± 62 | 714 ± 83.5 | 718 ± 58.4 | 713 ± 27.9 | 733 ± 44.8 |
|
Km (mM) |
0.68 ± 0.11a | 0.61 ± 0.15a | 0.39 ± 0.08b | 0.37 ± 0.04b | 0.33 ± 0.06b |
Uptake of 86Rb+ as a function of varying concentration of extracellular Rb+ (RbCl). As extracellular Rb+ concentration was increased, uptake of 86Rb+ was stimulated and subsequently became saturated in all groups. Kinetic parameters [maximal uptake (Vmax) and affinity (1/Km)] were obtained after the analysis of data with GraphPad Prism 7 software (GraphPad Software Inc., San Diego, CA, USA). Values are represented as mean ± SEM, n = 6. Values not sharing common superscripted letters are significantly different at p < 0.01.