Summary
Hippocampal place cells are spatially tuned neurons that serve as elements of a “cognitive map” in the mammalian brain1. To detect the animal’s location, place cells are thought to rely upon two interacting mechanisms: sensing the animal’s position relative to familiar landmarks2,3 and measuring the distance and direction that the animal has traveled from previously occupied locations4–7. The latter mechanism, known as path integration, requires a finely tuned gain factor that relates the animal’s self-movement to the updating of position on the internal cognitive map, with external landmarks necessary to correct positional error that accumulates8,9. Path-integration-based models of hippocampal place cells and entorhinal grid cells treat the path integration gain as a constant9–14, but behavioral evidence in humans suggests that the gain is modifiable15. Here we show physiological evidence from hippocampal place cells that the path integration gain is indeed a highly plastic variable that can be altered by persistent conflict between self-motion cues and feedback from external landmarks. In a novel, augmented reality system, visual landmarks were moved in proportion to the animal’s movement on a circular track, creating continuous conflict with path integration. Sustained exposure to this cue conflict resulted in predictable and prolonged recalibration of the path integration gain, as estimated from the place cells after the landmarks were extinguished. We propose that this rapid plasticity keeps the positional update in register with the animal’s movement in the external world over behavioral timescales. These results also demonstrate that visual landmarks not only provide a signal to correct cumulative error in the path integration system4,8,16–19, but also rapidly fine-tune the integration computation itself.
Path integration is an evolutionarily conserved strategy that allows an organism to maintain an internal representation of its current location by integrating over time a movement vector representing distance and direction traveled4–7. Place cells and entorhinal grid cells have been implicated as key components of a path integration system in the mammalian brain20–22. We recorded place cells from area CA1 (Extended Data Fig. 1) in 5 rats as they ran laps on a 1.5 m diameter circular track. The track was enclosed within a planetarium-style dome where an array of three visual landmarks was projected onto the interior surface to create an augmented reality environment (Fig. 1a,b). In contemporary virtual reality systems3,23–25, head- or body-fixed rats fictively locomote on a stationary air-cushioned ball or treadmill. Notwithstanding the flexibility of these systems to manipulate the visual experience of the animal, we built the dome apparatus to instead more completely preserve natural self-motion cues, such as vestibular, proprioceptive, and motor efference copy. This system enabled us to test the a priori hypothesis that manipulating the animal’s perceived movement speed relative to the landmarks results in a predictable recalibration of the path integration gain.
To create the visual illusion that the animal was running faster or slower, the array of landmarks was rotated coherently as a function of the animal’s movement speed. Movement of the landmarks was controlled by an experimental gain, G, which set the ratio between the rat’s travel distance with respect to the landmarks (landmark reference frame) and its travel distance along the stationary circular track (laboratory reference frame) (Fig. 1c). Recording sessions began with G = 1 (Epoch 1), a control condition with landmarks held stationary, so that the rat traveled the same distance in both the landmark and lab frames (Fig. 1d). The gain was then ramped over the course of multiple laps (Epoch 2) to values less than or greater than one. For G < 1, the landmarks moved at a speed proportional to (but slower than) the rat in the same direction; hence, the rat ran a shorter distance in the landmark frame than in the lab frame. For G > 1, the landmarks moved in the opposite direction; hence, the rat ran a greater distance in the landmark frame than in the lab frame. In Epoch 3, G was held at a steady-state target value (Gfinal). In some sessions, the landmarks were then extinguished (Epoch 4) to assess whether the effects of gain adjustment persisted in the absence of the landmarks.
Under gain-adjusted conditions, CA1 units (mean 7.2 ± 5.8 S.D. units/session) tended to fire in normal, spatially specific place fields when the firing was plotted in the landmark frame, but not when plotted in the lab frame (Fig. 1e). The strength and continuity of visual cue control over the place fields is highlighted by special cases of G (Fig. 2). As G was ramped down to 0, the place fields became increasingly large in the lab frame, eventually spanning multiple laps (Fig. 2a; Supplementary Video 1), but they maintained normal spatial selectivity in the landmark frame (Fig. 2b). At G = 0, the animal’s position became locked to the landmark frame, as the landmarks moved in precise register with the rat. Consequently, a unit that was active at that moment would typically remain active throughout Epoch 3, (e.g. yellow unit, Fig. 2a); in contrast, a unit that was inactive at that moment would typically remain silent throughout Epoch 3 (e.g. red unit, Fig 2a). When G was clamped at integer ratios such as 3/1 (Fig. 2c) or 1/2 (Fig. 2e), the units maintained the typical pattern of one field/lap in the landmark frame, while firing at the expected periodicity such as 3 times per lap (Fig. 2d) or every other lap (Fig. 2f) in the lab frame. Remapping events sometimes caused different populations of place cells to be active at different times. For example, place cells active during the initial part of the session sometimes went silent (loss of field; Fig. 2e, yellow unit), and place cells silent during the initial part of the session sometimes began firing at a preferred location (gain of field; Fig. 2e, red unit). The remapped cells exhibited normal place fields only in the landmark frame. These examples illustrate that the landmark array exercised robust control over the place fields, outweighing any subtle, local cues on the apparatus as well as nonvisual path integration cues, such as vestibular or proprioceptive cues.
To quantify the degree of landmark control over the population of recorded place cells, we developed a novel decoding algorithm that was robust to the remapping events described above. We estimated the gain, Hi, for each individual unit, i, by measuring its spatial frequency (i.e., the frequency of repetition of its spatially periodic firing pattern). The median value of Hi over all simultaneously recorded active units during a given set of laps was taken as a population estimate of the hippocampal gain, H, for those laps. Just as G quantifies the ratio between the rat’s travel distance in the landmark frame versus lab frame, H quantifies the ratio between the rat’s travel distance in the internal hippocampal “cognitive map” frame1 versus the lab frame. An ensemble coherence score for each unit was computed as the mean value over the session of | 1 - Hi / H |, measuring the deviation of Hi from H (Methods). The distribution of coherence scores (Fig. 2g) shows that Hi was within 2% of H for 80% (399/500) of individual units, and deviations greater than 5% were rare. Even when individual cells remapped, they still exhibited spatial periodicity at gain factors Hi that were close to H (see red and yellow units in Fig. 2c). Hence, the population of place cells acted as a rigidly coordinated ensemble from which a precise estimate of H could reliably be computed, despite occasional remapping by some place cells.
The degree of cue control in each session was quantified by the mean ratio H/G for Epochs 1–3 of a session; a ratio close to 1 indicates that the cognitive map was anchored to the landmark frame (i.e., G = H). The majority of sessions (83.33%) exhibited H/G near 1, but the rest showed substantially larger ratios (H/G > 1.1) indicating loss of landmark control (Fig. 2h; Extended Data Fig. 2). For sessions with H/G < 1.1, the spatial information per spike in the landmark frame far exceeded that in the lab frame (Fig. 2i). Further quantitative analyses was restricted to these sessions demonstrating ‘landmark control’. These results indicate that the augmented reality dome was successful in producing the desired illusion by strongly controlling the spatial firing patterns of the hippocampal cells in the majority of sessions (Extended Data Figs. 3, 4).
Despite strong cue control in the majority of sessions, place fields nonetheless tended to drift systematically by a small amount against the landmark frame on each successive lap (Extended Data Fig. 5; also visible in Figs. 2a,c,e and 3a,b) leading to total drifts of up to ~80° over the course of a session. The direction of this bias was consistent with a continuous conflict between the dynamic landmark reference frame and a path-integration-based estimate of position (although we cannot rule out the possible contribution of subtle uncontrolled external cues on the track or in the laboratory). That is, when path integration presumably undershot the landmark-defined location systematically (G < 1), the place fields shifted slightly backwards in the landmark frame; conversely, when path integration overshot the landmarks (G > 1), the place fields shifted forward. The shift may reflect a conflict resolution that is weighted heavily, but not completely, in the direction predicted by the landmark frame.
Given the apparent influence of path integration on place cells, revealed by systematic place-field drift despite strong landmark control, we tested whether anchoring of the cognitive map to the gain-altered landmark frame induced a recalibration of the path integrator that persisted in the absence of landmarks. Such recalibration would be evidenced by a predictable change in the hippocampal gain H when visual landmarks were extinguished (Fig. 1d, Epoch 4). The baseline hippocampal gain H was measured for each animal after extinguishing landmarks in sessions where the rat ran ~30 laps with stationary landmarks (G = 1). As expected, the baseline value of H was close to 1 (range 0.997 – 1.036). In subsequent gain manipulation sessions, if the path integrator circuit were unaltered, one would expect the place fields to revert to the lab frame (H ≈ 1) when landmarks were extinguished, as in the baseline sessions. Alternatively, if the path integrator gain were recalibrated perfectly, one would expect that the place fields would continue to fire as if the landmarks were still present and rotating at the final experimental gain (i.e., H ≈ Gfinal). We found that the hippocampal representation during Epoch 4 was intermediate between these extremes (Fig. 3a,b; Supplementary Video 2): there was a clear, linear relationship between Gfinal and the hippocampal gain H estimated during the first 12 laps after the landmarks were turned off (Fig. 3c). Moreover, this linear relationship was maintained when H was estimated during the next 12 laps Extended Data Fig. 6f). The values of H for the first and second 12 laps were highly correlated (Fig. 3d) with a slope near 1 (1.03). Thus, H was stable over at least 18 laps (i.e., the middle of the second estimation window). Despite this overall stability, there were still fluctuations in H in the absence of landmarks (Fig. 3e, Extended Data. Fig. 6). We tested whether changes in behavior could account for the hippocampal gain recalibration by computing several behavioral measures for each epoch (Extended Data, Behavioral Analysis). Multiple regression analysis showed that Gfinal strongly predicted H, whereas the behavioral variables had negligible influences on H (Extended Data Table 1).
Using a novel augmented reality dome apparatus, we show here that the path integration system employs a modifiable gain factor that can be recalibrated to a new value that can remain stable for at least several minutes in the absence of salient landmarks. Recalibration of this nature has been described extensively in other systems. The cerebellum plays a key role in recalibration of feedforward motor commands26. Similarly, the gain of the vestibulo-ocular reflex adapts to changes in the magnitude of retinal slip caused by magnifying glasses, an effect that persists even after the glasses are removed27. As with our own results, the recalibration is not perfect in these motor adaptation tasks; i.e., the gain measured after the training trials are biased towards, but not precisely the same as, the experimental gain implemented during the training trials. To our knowledge, such gain recalibration has not been demonstrated physiologically in cognitive phenomena such as spatial representation and path integration (but see15). The lack of complete recalibration may be due to an insufficient number of training laps during Epoch 3, or may reflect inherent limits on the plasticity of the path integrator gain variable.
It is widely accepted that visual landmarks provide a signal to correct error that accumulates during path integration28. The results in this paper demonstrate physiological evidence for a role of vision in the path integration computation itself by providing an error signal analogous to retinal slip in the VOR27. Specifically, this error signal fine-tunes the gain of the path integrator15, minimizing the accumulation of error in the first place. Although recalibration of the path integrator gain may be expected over developmental time scales, these results indicate that the path integration gain is fine-tuned even at behavioral time scales. This fine-tuning may be required to (a) maintain accuracy of the path integration signal under different behavioral conditions (e.g., locomotion on different surfaces that provide varying degrees of slip and cause alterations in the self-motion inputs to the path integrator); (b) synchronize the different types of self-motion signals (e.g., vestibular, optic flow, motor copy, or proprioception) thought to underlie path integration; and (c) coordinate the discrete set of different path integration gains thought to underlie the expansion of grid scales along the dorsal-ventral axis of the medial entorhinal cortex12,29,30. The recalibration might be implemented by changes to the head direction31 or speed32,33 signals that provide input to a path integration circuit. Alternatively, these representations may be unaltered and the gain changes are implemented by changing the synaptic weights between the inputs and putative attractor networks that perform the path integration9–11,13. The augmented reality system described here will allow the investigation of mechanisms underlying the interaction between external sensory input and the internal neural dynamics at the core of the path integration system.
Extended Data
Extended Data Table 1:
Mean vel (°/sec) | Pauses/lap | Pause Duration (s) | Interpause Interval (s) | Interpause Distance (°) | Gfinal | |
---|---|---|---|---|---|---|
Mean (S.E.M.) | ||||||
Epoch 1 | 24.6 (0.7) | 0.9 (0.2) | 8.8 (1.0) | 55.8 (8.2) | 887 (136) | -- |
Epoch 2 | 25.2 (0.9) | 1.0 (0.1) | 6.5 (0.5) | 61.8 (18.0) | 1119 (399) | -- |
Epoch 3 | 25.0 (1.0) | 1.5 (0.2) | 8.8 (1.0) | 26.3 (3.5) | 461 (79) | -- |
Epoch 4 | 24.2 (1.0) | 1.5 (0.3) | 9.2 (0.8) | 34.9 (9.4) | 531 (125) | -- |
Epochs 3–1 | 0.4 (0.5) | *0.5 (0.2) | 0(1.5) | *−29.6 (7.9) | *−426 (121) | -- |
Epochs 4–3 | −0.8 (0.4) | 0.1 (0.2) | 0.3 (1.3) | 8.6 (9.8) | 69 (134) | -- |
Multiple regression | ||||||
EDOCh 4 - EDOCh 3 | ||||||
β | −0.01 | 0 | 0 | 0 | 0 | 0.65 |
S.E. | 0.01 | 0.02 | 0 | 0 | 0 | 0.05 |
EDOCh 3 - Epoch 1 | ||||||
β | 0.01 | −0.01 | 0.01 | 0 | 0 | 0.66 |
S.E. | 0.01 | 0.03 | 0 | 0 | 0 | 0.05 |
Two-sided Wilcoxon Signed Rank tests were performed on the differences between values in Epochs 3 and 1 and Epochs 4 and 3 with null hypothesis that the difference = 0. Pauses/lap (n = 37 sessions; p = 0.035); Interpause Interval (n = 37 sessions; p = 0.001); Interpause Distance (n = 37 sessions; p = 0.003). All other tests for Epochs 3–1 and Epochs 4–3 were not significant
Supplementary Material
Acknowledgments.
We thank Bill Nash and Bill Quinlan for assistance with constructing the apparatus; Marissa Ferreyros, Macauley Breault, Nick Lukish, Jeremy Johnson, Balazs Vagvolgyi and Douglas GoodSmith for technical assistance in running experiments; Geeta Rao, Vyash Puliyadi, Cheng Wang, Heekyung Lee, Robert Nickl, Adrian Haith and Jonathan Bohren for discussions and technical advice. This research was supported by NIH grants R01 MH079511 (HTB, JJK), R21 NS095075 (NJC, JJK), and R01 NS102537 (NJC, JJK, FS), a JHU Discovery Award (NJC, JJK), a JHU Science of Learning Institute Award (JJK, NJC), a JHU Kavli NDI Postdoctoral Distinguished Fellowship (MSM) and a JHU Mechanical Engineering Departmental Fellowship (RPJ).
Footnotes
The authors declare that they have no competing financial or non-financial interests.
References
- 1.O’Keefe J & Nadel L The Hippocampus as a Cognitive Map (Oxford University Press, 1978). [Google Scholar]
- 2.Acharya L et al. Causal Influence of Visual Cues on Hippocampal Article Causal Influence of Visual Cues on Hippocampal Directional Selectivity. Cell 164, 197–207 (2016). [DOI] [PubMed] [Google Scholar]
- 3.Chen G, King J a, Burgess, N. & O’Keefe, J. How vision and movement combine in the hippocampal place code. Proc. Natl. Acad. Sci. U. S. A 110, 378–83 (2013). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 4.Etienne AS & Jeffery KJ Path integration in mammals - Etienne - 2004 - Hippocampus - Wiley Online Library. Hippocampus (2004). at <http://onlinelibrary.wiley.com/doi/10.1002/hipo.10173/full%5Cnpapers2://publication/uuid/BE99CA4B-E84A-4A34-A3F6-D924E35CA5B6> [DOI] [PubMed] [Google Scholar]
- 5.Wehner R & Menzel R Do Insects Have Cognitive Maps? Annu. Rev. Neurosci 13, 403–414 (1990). [DOI] [PubMed] [Google Scholar]
- 6.Wittlinger M, Wehner R & Wolf H The ant odometer: Stepping on stilts and stumps. Science (80-.) 312, 1965–1967 (2006). [DOI] [PubMed] [Google Scholar]
- 7.Mittelstaedt ML & Mittelstaedt H Homing by path integration in a mammal. Naturwissenschaften 67, 566–567 (1980). [Google Scholar]
- 8.Gallistel CR The organization of learning Cambridge: Bradford Books / MIT Press; (1990). [Google Scholar]
- 9.Samsonovich a & McNaughton BL. Path integration and cognitive mapping in a continuous attractor neural network model. J. Neurosci 17, 5900–5920 (1997). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 10.Fuhs MC & Touretzky DS A spin glass model of path integration in rat medial entorhinal cortex. J. Neurosci 26, 4266–4276 (2006). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 11.McNaughton BL, Battaglia FP, Jensen O, Moser EI & Moser MB Path integration and the neural basis of the ‘cognitive map’. Nat Rev Neurosci 7, 663–78 (2006). [DOI] [PubMed] [Google Scholar]
- 12.Hasselmo ME, Giocomo LM & Zilli EA Grid cell firing may arise from interference of theta frequency membrane potential oscillations in single neurons. Hippocampus 17, 1252–1271 (2007). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 13.Blair HT, Gupta K & Zhang K Conversion of a phase- to a rate-coded position signal by a three-stage model of theta cells, grid cells, and place cells. Hippocampus 18, 1239–55 (2008). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 14.Burgess N, Barry C & O’Keefe J An oscillatory interference model of grid cell firing. Hippocampus 812, 801–812 (2007). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 15.Tcheang L, Bulthoff HH & Burgess N Visual influence on path integration in darkness indicates a multimodal representation of large-scale space. Proc. Natl. Acad. Sci 108, 1152–1157 (2011). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 16.Knierim JJ, Kudrimoti HS & McNaughton BL Interactions Between Idiothetic Cues and External Landmarks in the Control of Place Cells and Head Direction Cells. J. Neurophysiol 80, 425–446 (1998). [DOI] [PubMed] [Google Scholar]
- 17.Zugaro MB, Arleo A, Berthoz A & Wiener SI Rapid spatial reorientation and head direction cells. J. Neurosci 23, 3478–3482 (2003). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 18.Hardcastle K, Ganguli S & Giocomo LM Environmental Boundaries as an Error Correction Mechanism for Grid Cells. Neuron 86, 827–839 (2015). [DOI] [PubMed] [Google Scholar]
- 19.Etienne AS, Maurer R & Séguinot V Path integration in mammals and its interaction with visual landmarks. J. Exp. Biol 199, 201–209 (1996). [DOI] [PubMed] [Google Scholar]
- 20.Moser EI, Moser M-B & McNaughton BL Spatial representation in the hippocampal formation: a history. Nat. Neurosci 20, 1448–1464 (2017). [DOI] [PubMed] [Google Scholar]
- 21.Gil M et al. Impaired path integration in mice with disrupted grid cell firing. Nat. Neurosci 21, 81–93 (2018). [DOI] [PubMed] [Google Scholar]
- 22.Tennant SA et al. Stellate Cells in the Medial Entorhinal Cortex Are Required for Spatial Learning. Cell Rep 22, 1313–1324 (2018). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 23.Hölscher C, Schnee A, Dahmen H, Setia L & Mallot HA Rats are able to navigate in virtual environments. J. Exp. Biol 208, 561–9 (2005). [DOI] [PubMed] [Google Scholar]
- 24.Harvey CD, Collman F, Dombeck DA & Tank DW Intracellular dynamics of hippocampal place cells during virtual navigation. Nature 461, 941–946 (2009). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 25.Ravassard P et al. Multisensory Control of Hippocampal Spatiotemporal Selectivity. Science 1–7 (2013). doi: 10.1126/science.1232655 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 26.Bastian AJ Learning to predict the future: the cerebellum adapts feedforward movement control. Curr. Opin. Neurobiol 16, 645–649 (2006). [DOI] [PubMed] [Google Scholar]
- 27.Miles FA & Lisberger SG Plasticity in the vestibulo-ocular reflex: a new hypothesis. Annu. Rev. Neurosci 4, 273–99 (1981). [DOI] [PubMed] [Google Scholar]
- 28.Etienne a S., Maurer R & Séguinot V. Path integration in mammals and its interaction with visual landmarks. J. Exp. Biol 199, 201–9 (1996). [DOI] [PubMed] [Google Scholar]
- 29.Terrazas A et al. Self-motion and the hippocampal spatial metric. J. Neurosci 25, 8085–96 (2005). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 30.Maurer AP, VanRhoads SR, Sutherland GR, Lipa P & McNaughton BL Self-motion and the origin of differential spatial scaling along the septo-temporal axis of the hippocampus. Hippocampus 15, 841–852 (2005). [DOI] [PubMed] [Google Scholar]
- 31.Cullen KE & Taube JS Our sense of direction: Progress, controversies and challenges. Nat. Neurosci 20, 1465–1473 (2017). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 32.Kropff E, Carmichael JE, Moser M-B & Moser EI Speed cells in the medial entorhinal cortex. Nature 523, 419–424 (2015). [DOI] [PubMed] [Google Scholar]
- 33.Hinman JR, Brandon MP, Climer JR, Chapman GW & Hasselmo ME Multiple Running Speed Signals in Medial Entorhinal Cortex. Neuron 91, 666–679 (2016). [DOI] [PMC free article] [PubMed] [Google Scholar]
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