Arctium Species Secondary Metabolites Chemodiversity and Bioactivities

Abstract

Arctium species are known for a variety of pharmacological effects due to their diverse volatile and non-volatile secondary metabolites. Representatives of Arctium species contain non-volatile compounds including lignans, fatty acids, acetylenic compounds, phytosterols, polysaccharides, caffeoylquinic acid derivatives, flavonoids, terpenes/terpenoids and volatile compounds such as hydrocarbons, aldehydes, methoxypyrazines, carboxylic and fatty acids, monoterpenes and sesquiterpenes. Arctium species also possess bioactive properties such as anti-cancer, anti-diabetic, anti-oxidant, hepatoprotective, gastroprotective, antibacterial, antiviral, antimicrobial, anti-allergic, and anti-inflammatory effects. This review aims to provide a complete overview of the chemistry and biological activities of the secondary metabolites found in therapeutically used Arctium species. Summary of pharmacopeias and monographs contents indicating the relevant phytochemicals and therapeutic effects are also discussed, along with possible safety considerations.

Introduction

Botanical and Ethnobotanical Aspects

The genus Arctium L. (Asteraceae/Compositae, tribe Cardueae, subtribe Carduinae), together with the related genera Cousinia Cass., Hypacanthium Juz. and Schmalhausenia C. Winkl, forms the so-called Arctium–Cousinia group (de Souza et al., 2004). The species of the Arctium genus, also known as ‘burdock,’ comprise biennial herbs occurring in waste places, streams and roadsides, less often in wood and forests, in temperate regions of Europe and Asia and sporadically in subtropical and tropical regions (European Scientific Cooperative on Phytotherapy, 2003). In North and South America, the genus is considered as naturalized, whereas in Africa it is quite rare. The name of the genus comes from the Greek ‘arcteion’ which means ‘bear,’ alluding to the plant habitus characterized by pronounced hairiness.

According to the Plant List¹, this genus encompasses 18 recognized species among which five are considered as hybrid species due to the frequent outbreeding occurring between its allogamous representatives (Lopez-Vinyallonga et al., 2010).

Arctium species are represented by hemicryptophyte plants equipped with a stout, erect taproot and entire (sporadically as dentate), rough, unarmed, alternate, tomentose, and cordate leaves. The stem is usually stout, erect, grooved, branched, and reddish. Inflorescences are formed by solitary or corymbose ovoid-conical to spherical capitula equipped with involucres made up of bracts ending with hooked apices. Receptacles are composed of numerous, hard scales. Florets are only tubulose, hermaphrodite, purple or white. Pollination is allowed by insects, mostly belonging to Lepidoptera. Fruits are having oblong, rugose achenes equipped with a golden-yellow pappus (European Scientific Cooperative on Phytotherapy, 2003).

The Arctium genus is highly polymorphic due to variability occurring in hairiness of leaves and capitula, length of floral peduncles, and color of capitula and florets. As a consequence, a sharp distinction between its members cannot occasionally be defined. In the Euro-Mediterranean area, six main species are found: A. atlanticum (Pomel) H. Lindb., A. lappa L., A. minus (Hill) Bernh., A. nemorosum Lej., A. palladini (Marcow) R.E.Fr. & Soderb. and Arctium tomentosum Mill. (European Scientific Cooperative on Phytotherapy, 2003; Figure 1).

FIGURE 1.

Plants of Arctium. (A) Arctium lappa; (B) Arctium minus; (C) Arctium tomentosum; (D) Arctium nemorosum.

Arctium lappa enjoys a longstanding use in the traditional medicine (mainly roots, and, to a lesser extent, leaves and seeds) due to the bioactive properties of its metabolites (Zhao et al., 2014). To the best of our knowledge, the most utilized species for therapeutic purposes, are on first place A. lappa, also known as ‘greater burdock,’ and, to a minor extent, A. minus (lesser burdock) and A. tomentosum (wooly burdock).

Arctium lappa is an herbaceous biennial plant up to 150 cm tall, with pubescent to subglabrous epigeal parts. Basal leaves are up to 50 cm in diameter, ovate, cordate and with hollow petioles. Stems are branched and end with corymbose capitula. Florets are as long as the involucral bracts. A. minus differs for the shape of inflorescence (solitary terminal capitula), dimensions of involucral bracts and capitula (smaller and shorter, respectively) and consistency of petiole (hollow). Furthermore, the bracts show a relatively hinted hairiness. A. tomentosum is characterized by petioles and peduncles covered with wooly tufts; petioles are solid. The involucral bracts are similar in dimensions to those of A. minus, but they show a dense covering of hairs. Florets are longer than bracts as in A. minus (European Scientific Cooperative on Phytotherapy, 2003). These three species are quite common in central Europe where often they undergo interspecific hybridization giving rise to questions about their integrity (European Scientific Cooperative on Phytotherapy, 2003).

Greater burdock (A. lappa) has been traditionally used in both Asian and European medicines as depurative, diuretic, carminative, anti-inflammatory, and anti-tubercular agent (Zhao et al., 2014). For therapeutic purposes, its different parts such as roots, fruits, and leaves are used. The latter have been used to treat ulcers and fester wounds (Jaric et al., 2007). They are also applied externally on the forehead to cure headache and fever, on the scalp to treat bruises and hair loss, mixed with oil and honey and applied on the chest to heal cough. In addition, under infusion, they are taken orally to treat enuresis in children (Pieroni et al., 2011). Fruits of burdock (Arctii fructus) are used to purify the blood (Lans and Turner, 2011) and to treat respiratory and infectious diseases (Bai et al., 2016). In addition, A. lappa roots, together with aerial parts of Rumex acetosella L., leaves of Ulmus rubra Muhl. and rhizomes of Rheum officinale Baill., are used to make ‘Essiac,’ a tea used by the Ojibwa tribe of Canada for the treatment of cancer (Leonard et al., 2006). In the veterinary medicine, the root is used to treat mastitis (Lans et al., 2007), whereas the whole plant is applied against endoparasites in poultry (Lans and Turner, 2011). Besides therapeutic uses, A. lappa is also appreciated as an edible plant. For the latter purpose, young leaves, and stalks are eaten raw or cooked (Pieroni et al., 2011).

Lesser burdock (A. minus) leaves are traditionally used externally to treat rheumatic pains, fever, sunstroke, wounds, general infections, skin and body inflammations, alopecia, and bladder diseases (de Souza et al., 2004; Erdemoglu et al., 2009; Neves et al., 2009). They are also disposed above the body of the patient, wetted with vinegar or milk, to stimulate sweating (Sezik et al., 2001). Roots and leaves, under infusion, are also used against snake and scorpion bites and to purify the blood (Mosaddegh et al., 2012). Basal leaves and stems are also eaten raw as a snack or stewed (Tardio et al., 2005). Due to their bitter taste, they are also used to stimulate the appetite and liver functions (Tardio et al., 2005).

Wooly burdock (A. tomentosum) leaves are used as vulnerary, to treat skin rash, ulcers, abscesses, mouth sores and against rheumatic pains, whereas root is applied against alopecia and to wash hairs (Sezik et al., 2004; Saric-Kundalic et al., 2010). Roots are also employed to make a tea used for digestive problems, ulcers, rheumatisms, to purify the blood and increase sweating and as diuretic (Saric-Kundalic et al., 2010).

Medicinal Uses of Arctium Species in Pharmacopeias and Monographs

Burdock species and in particular A. lappa are used in traditional medicine for different purposes. The main traditional use of the roots of A. lappa in Europe comprises treatment of dermatological disorders (Saric-Kundalic et al., 2010; Miglani and Manchanda, 2014) whereas in other Eastern and Asian countries A. lappa fruits and roots are used as an antidiabetic remedy (Tousch et al., 2014; Xu et al., 2014, 2015; Ahangarpour et al., 2017). In Traditional Chinese Medicine (TCM), apart from the antidiabetic activity, the roots of A. lappa are considered as a blood detoxifying agent (Qin et al., 2014). In the Japanese pharmacopeia, the fruit is included as a traditional herbal medicine with recent studies revealing the potential of its extracts in oncology (Ikeda et al., 2016). The leaves of A. lappa have also been reported as an anti-inflammatory agent to relieve gastrointestinal disorders in Brazilian traditional medicine (de Almeida et al., 2013).

According to international institutions that work in the validation of traditional herbal medicines, such as the European Medicines Agency (EMA) and the European Scientific Cooperative on Phytotherapy (ESCOP), A. lappa is recommended and approved for different indications. For example, the EMA monograph approves the use of roots of A. lappa, A. minus, and A. tomentosum as an adjuvant in minor urinary tract complaints, in temporary loss of appetite and for seborrheic skin conditions (European Medicines Agency, 2011). All these indications are based upon long-standing use. In 2016, ESCOP released a monograph where the roots of all the former three species are indicated to be internally and externally used for seborrheic skin, eczema, furuncles, acne, psoriasis and internally for minor urinary tract disorders (European Scientific Cooperative on Phytotherapy-The Scientific Foundation for Herbal Medicinal Products, 2016). For oral and internal administration, the herbal drug can be used as an infusion, extract, tincture or decoction but the fresh pulp of the roots or a decoction can also be directly applied to the skin. The later monograph reveals that A. lappa preparations should not be ingested during pregnancy, lactation, or in case of hypersensitivity to the Compositae and in patients with oedema due to impaired heart or kidney function. Although certain preclinical studies can be found in the literature, clinical trials are not available for these indications approved by ESCOP and EMA.

Phytochemistry

Non-volatile Compounds

Till date, more than two hundred non-volatile compounds have been isolated from Arctium genus. These chemical compounds include lignans, terpenoids, sterols, flavonoids, phenolics, lactones, polyacetylenes, quinic acids, and sugars (polysaccharides). In particular, lignans are the most characteristic components in the Arctium genus. The details of chemical compounds, their occurrence in different plant parts, and the analytical methods used for their quali-quantitative determinations are briefly summarized in Table 1 whereas their description is provided in this section. The chemical structures of some compounds from Arctium species are shown in Figure 2.

Table 1.

The known non-volatile constituents of Arctium species.

No	Compound name	Formula	Species	Plant origin/part	Analytical method	References
	Lignans
1	Diarctigenin	C42H46O12	A. lappa	Fruits, roots, seeds	IR/NMR/MS/TLC	Han et al., 1994; Park et al., 2007; Qin et al., 2014
2	Arctiin	C27H34O11	A. lappa, A. tomentosum	Leaves, fruits, roots, seeds	UV/IR/MS/NMR/HPLC/LCMS/ MALDI-QIT-TOF MS	Wang and Yang, 1993; Ting-Guo et al., 2001; Yu et al., 2003; Ming et al., 2004; Liu et al., 2005, 2012, 2015; Wang et al., 2005; Matsumoto et al., 2006; Boldizsar et al., 2010; Ferracane et al., 2010; Zhou et al., 2011; Qin et al., 2014; Su et al., 2015; Lou et al., 2016; Al-Shammaa et al., 2017
3	Arctigenin	C12H24O7	A. lappa, A. tomentosum	Leaves, fruits, seeds, roots	UV/MS/NMR/HPLC/LCMS/ MALDI-QIT-TOF MS/ HRESI-MS	Umehara et al., 1993; Wang and Yang, 1993; Liu et al., 2005, 2012, 2015; Matsumoto et al., 2006; Gao et al., 2008; Boldizsar et al., 2010; Ferracane et al., 2010; Predes et al., 2011; Zhou et al., 2011; Qin et al., 2014; Su et al., 2015; Al-Shammaa et al., 2017
4	Arctigenin-4-O-β-D-gentiobioside	C18 H32 O16	A. lappa	Fruits	NMR/UV/IR/ORD/HRESIMS	Yang et al., 2015
5	Arctigenin-4-O-α-D-galactopyranosyl-(1→6)-O-β-D-glucopyranoside	C18 H32 O16	A. lappa	Fruits	NMR/UV/IR/ORD/HRESIMS	Yang et al., 2015
6	Arctigenin-4-O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside	C32 H42 O15	A. lappa	Fruits	NMR/UV/IR/ORD/HRESIMS	Yang et al., 2015
7	3-benzyl-6-(1-hydroxyethyl)-2,5-piperazinedione	C13 H16 N2O3	A. lappa	Fruits	IR/HR-ESI-MS/NMR/CD	Yang et al., 2012
8	3-benzyl-2,5- piperazinedione	C13H16N2O2	A. lappa	Fruits	IR/HR-ESI-MS/NMR/CD	Yang et al., 2012
9	5′-propanediolmatairesinoside	C29 H38O13	A. lappa	Fruits	NMR/UV/IR/ORD/HRESIMS	Yang et al., 2015
10	(7′R,8R,8’R)-rafanotrachelogenin-4-O-β-D-glucopyranoside	C27 H34 O12	A. lappa	Fruits	NMR/UV/IR/ORD/HRESIMS	Yang et al., 2015
11	(7′S,8R,8′R)-rafanotrachelogenin-4-O-β-D-glucopyranoside	C27 H34 O12	A. lappa	Fruits	NMR/UV/IR/ORD/HRESIMS	Yang et al., 2015
12	(7S,8S,8’R)-4,7-dihydroxy-3,3’,4-trimethoxyl-9-oxo benzylbutyrolactone lignan-4-O-β-D-glucopyranoside	C27 H34O12	A. lappa	Fruits	NMR/UV/IR/ORD/HRESIMS	Yang et al., 2015
13	(7S,8S,8’R)-4,7- dihydroxy-3,3′,4′-trimethoxyl-9-oxo dibenzylbutyrolactone lignin	C21H24O7	A. lappa	Fruits	NMR/UV/IR/ORD/HRESIMS	Yang et al., 2015
14	(7R,8S,8′R)-4,7,4′-trihydroxy-3,3′- dimethoxyl-9-oxo dibenzylbutyrolactone lignan-4-O-β-D-glucopyranoside	C26 H32O12	A. lappa	Fruits	NMR/UV/IR/ORD/HRESIMS	Yang et al., 2015
15	7,8-didehydroarctigenin	C21H22O5	A. lappa	Fruits	HRFAB/EIMS/NMR	Matsumoto et al., 2006
16	Arctiidilactone	C20H20O8	A. lappa	Fruits	NMR/UV/IR/ORD/HRESIMS	Yang et al., 2015
17	Arctiiapolignan A	C20H28O10	A. lappa	Fruits	NMR/UV/IR/ORD/HRESIMS	Yang et al., 2015
18	Arctiisesquineolignan A	C42H52O19	A. lappa	Fruits	NMR/UV/IR/ORD/HRESIMS	Yang et al., 2015
19	Arctiisesquineolignan B	C36H46O16	A. lappa	Fruits	UV/IR/HRESIMS/NMR	He etal., 2016
20	Arctiiphenolglycoside A	C19H28O13	A. lappa	Fruits	UV/IR/HRESIMS/NMR	He etal., 2016
21	Arctignan A	C30H34O10	A. lappa	Seeds	UV/MS/NMR/HPLC	Umehara et al., 1993
22	Arctignan B	C30H34O10	A. lappa	Seeds	UV/MS/NMR/HPLC	Umehara et al., 1993
23	Arctignan C	C30H32O10	A. lappa	Seeds	UV/MS/NMR/HPLC	Umehara et al., 1993
24	Arctignan D	C30H34O10	A. lappa	Seeds	UV/MS/NMR/HPLC/LCMS/ MALDI-QIT-TOF MS	Umehara et al., 1993; Liu et al., 2012
25	Arctignan E	C40H44O13	A. lappa	Seeds	UV/IR/MS/NMR/HPLC	Umehara et al., 1993; Ming et al., 2004; Ferracane et al., 2010; Qin et al., 2014
26	Lappaol A	C30H32O9	A. lappa, A. tomentosum	Seeds/fruits	TLC/UV/IR/MS/NMR/HPLC	Ichihara et al., 1976; Ting-Guo et al., 2001; Ming et al., 2004; Ferracane et al., 2010; Liu et al., 2012; Qin et al., 2014; Su et al., 2015
27	Lappaol B	C31H34O9	A. lappa	Seeds/fruits	NMR/MS/TLC/HPLC	Ichihara et al., 1976; Qin et al., 2014
28	Isolappaol C	C30H34O10	A. lappa, A. tomentosum	Seeds/fruits	NMR/MS/TLC	Park et al., 2007; Qin et al., 2014; Su et al., 2015
29	Lappaol C	C30H34O10	A. lappa	Seeds	TLC/UV/IR/MS/NMR	Ichihara et al., 1977; Ting-Guo et al., 2001; Ming et al., 2004; Park et al., 2007; Ferracane et al., 2010; Liu et al., 2012; Su et al., 2015
30	Lappaol D	C31H36O10	A. lappa	Seeds	NMR/MS/TLC	Ichihara et al., 1977; Park et al., 2007
31	Lappaol E	C30H34O10	A. lappa	Seeds	NMR/MS/TLC	Ichihara et al., 1977; Park et al., 2007
32	Lappaol F	C42H46O12	A. lappa, A. tomentosum	Fruits, seeds	TLC/UV/IR/MS/NMR/HPLC	Ting-Guo et al., 2001; Ming et al., 2004; Park et al., 2007; Ferracane et al., 2010; Qin et al., 2014
33	Lappaol H	C40H46O14	A. lappa	Seeds/fruits	UV/MS/NMR/HPLC/LCMS/ MALDI-QIT-TOF MS	Liu et al., 2012; Qin et al., 2014
34	Neoarctin A	C42H46O12	A. lappa	Seeds	UV, IR, 1H-NMR, 13C-NMR, DEPT, 2D-NMR and MS	Wang and Yang, 1995; Yong et al., 2007
35	Neoarctin B	C42H46O12	A. lappa	Seeds	UV, IR, 1H-NMR, 13C-NMR, DEPT, 2D-NMR and MS	Wang and Yang, 1993
36	Matairesinoside	C26H32O11	A. lappa	Fruits	UV/IR/HPLC	Boldizsar et al., 2010
37	Matairesinol	C20H22O6	A. lappa	Seeds/fruits	UV/MS/NMR/HPLC/LCMS/ MALDI-QIT-TOF MS	Wang and Yang, 1993; Boldizsar et al., 2010; Ferracane et al., 2010; Liu et al., 2012; Qin et al., 2014; Su et al., 2015
38	Matairesinol-4,4′-di-O-β-D-glucopyranoside	C27 H34 O12	A. lappa	Fruits	NMR/UV/IR/ORD/HRESIMS	Yang et al., 2015
39	Pinoresinol	C20H22O6	A. lappa	Fruits	UV/IR/HPLC	Boldizsar et al., 2010
40	Phylligenin	C21H24O6	A. lappa	Fruits	UV/IR/HPLC	Boldizsar et al., 2010
41	Styraxlignolide E	C26H32O11	A. lappa	Fruits	NMR/UV/IR/ORD/HRESIMS	Yang et al., 2015
42	Styraxlignolide D	C26H32O11	A. lappa	Fruits	NMR/UV/IR/ORD/HRESIMS	Yang et al., 2015
43	Syringaresinol	C22H26O8	A. lappa	Roots	UV/IR/ESIMS/NMR	Han et al., 2013
44	(7S, 8R)-4,7,9,9′-tetrahydroxy-3,3′-dimethoxyl-7′-oxo-8-4′-oxyneolignan-4-O-β-D-glucopyranoside	C26 H34 O13	A. lappa	Roots	IR/HR-ESI-MS/NMR/CD	Yang et al., 2012
45	(7′S, 8′R, 8S)-4,4′,9′-trihydroxy-3,3′-dimethoxy-7′,9-epoxylignan-7-oxo-4-O-β-D-glucopyranosyl-4′-O-β-D-glucopyranoside	C32 H42 O17	A. lappa	Roots	IR/HR-ESI-MS/NMR/CD	Yang et al., 2012
46	(7S, 8R)-4,7,9,9′-tetrahydroxy-3,3′dimethoxy-8-O-4′-neolignan-9′-O-β-D-apiofuranosyl-(1 → 6)-O-β-D-glucopyranoside	C31H44O16	A. lappa	Fruits	IR/HR-ESI-MS/NMR/CD	Huang K. et al., 2015; Huang X.Y. et al., 2015
47	(8R)-4,9,9′-trihydroxy-3,3′-dimethoxy-7-oxo-8-O-4′-neolignan-4-O-β-D-glucopyranoside	C26 H34 O12	A. lappa	Fruits	IR/HR-ESI-MS/NMR/CD	Huang K. et al., 2015; Huang X.Y. et al., 2015
48	(7R, 8S)-dihydrodehydrodiconiferyl alcohol-7′-oxo-4-O-β-D-glucopyranoside	C26 H32 O12	A. lappa	Fruits	IR/HR-ESI-MS/NMR/CD	Huang K. et al., 2015; Huang X.Y. et al., 2015
49	(7′S, 8′R, 8S)-4,4′,9′-trihydroxy-3,3′-dimethoxy-7′,9-epoxylignan-7-oxo-4-O-β-D-glucopyranoside	C26 H32 O12	A. lappa	Fruits	IR/HR-ESI-MS/NMR/CD	Huang K. et al., 2015; Huang X.Y. et al., 2015
50	Trachelogenin	C21H24O7	A. lappa	Fruits	–	Ichikawa et al., 1986
	Terpenes/Terpenoids
51	β-eudesmol	C15H26O	A. lappa	Fruits	–	Yayli et al., 2005
52	Ursolic acid	C30H48O3	A. lappa	Root	UV/IR/ESIMS/NMR	Han et al., 2013
53	Oleanolic acid	C30H48O3	A. lappa	Roots	UV/IR/ESIMS/NMR	Han et al., 2013
54	Arctiopicrin	C19H26O6	A. lappa, A. minus	Leaves	TLC/NMR	Savina et al., 2006
55	Onopordopicrin	C19H24O6	A. lappa, A. nemorosum	Leaves/aerial parts	TLC/HPLC/NMR/HR-ESI-TOF-MS	Barbosa et al., 1993; Savina et al., 2006; Machado et al., 2012; Zimmermann et al., 2012
56	Dehydrovomifoliol	C13H18O3	A. lappa	Leaves	NMR/HR-ESI-TOF-MS	Machado et al., 2012
57	Loliolide	C11H16O3	A. lappa	Leaves	NMR/HR-ESI-TOF-MS	Machado et al., 2012
58	Dehydromelitensin-8-(4′-hydroxymethacrylate)	C15 H24 O6	A. lappa	Leaves	NMR/HR-ESI-TOF-MS	Machado et al., 2012
59	Dehydromelitensin	C15H20O4	A. lappa	Leaves	NMR/HR-ESI-TOF-MS	Machado et al., 2012
60	Melitensin	C15H22O4	A. lappa	Leaves	NMR/HR-ESI-TOF-MS	Machado et al., 2012
61	3α-acetoxyhop-22(29)-ene	C30H49O2	A. lappa	Leaves	NMR, IR and MS	Jeelani and Khuroo, 2012
62	3α-hydroxylanosta-5,15-diene	C30 H50O	A. lappa	Leaves	NMR, IR and MS	Jeelani and Khuroo, 2012
	Flavonoids
63	Baicalin	C21H18O11	A. lappa			Uchiyama et al., 2005
64	Luteolin	C25H24O12	A. lappa	Leaves/roots	UPLC/LC/MS/MS	Ferracane et al., 2010; Lou et al., 2010a; Tang et al., 2014
65	Rutin	C27H30O16	A. lappa, A. minus	Leaves	TLC/UPLC/LC/MS/MS	Saleh and Bohm, 1971; Lou et al., 2010a,b
66	Quercitrin	C21H20O11	A. lappa	Leaves/roots	UPLC/LC/MS/MS	Lou et al., 2010a
67	Quercetin	C15H10O7	A. lappa	Leaves/roots	UPLC/LC/MS/MS/HRESI-MS	Lou et al., 2010a; Predes et al., 2011; Tang et al., 2014
68	Quercetin 3-O-glucuronide	C21H18O13	A. lappa	Roots	HPTLC/LC/ESI–MS/MS	Rajasekharan et al., 2015
69	Quercetin 3-vicianoside	C26H28O16	A. lappa	Roots	HPTLC/LC/ESI–MS/MS	Rajasekharan et al., 2015
70	Quercetin rhamnoside	C21H20O11	A. lappa	roots	HPLC/LC/MS/MS	Ferracane et al., 2010
71	Quercimeritrin	C21H20O12	A. minus	Leaves	TLC	Saleh and Bohm, 1971
72	Isoquercetin	C21H20O12	A. minus	Leaves	TLC	Saleh and Bohm, 1971
73	Astragalin	C21H20O11	A. minus	Leaves	TLC	Saleh and Bohm, 1971
74	Kaempferol-3-o-rhamnoglucoside	C27 H30 O15	A. minus	Leaves	TLC	Saleh and Bohm, 1971
75	Biachanin A	C16H12O5	A. lappa	Roots	–	Tamayo et al., 2000; Eberding et al., 2007
76	Genestein	C15H10O5	A. lappa	Roots	–	Tamayo et al., 2000; Eberding et al., 2007
77	Nobiletin	C21H22O8	A. lappa	Roots	–	Tamayo et al., 2000; Eberding et al., 2007
78	Tangeretin	C20H20O7	A. lappa	Roots	–	Tamayo et al., 2000
	Sterols
79	β-sitosterol	C29H50O	A. lappa, A. tomentosum	Seeds/roots/fruits	UV/IR/MS/NMR/HPLC	Ting-Guo et al., 2001; Ming et al., 2004; Han et al., 2013
80	Sitosterol-beta-D-glucopyranoside	C35 H60 O6	A. lappa	Roots	IR/NMR/EI-MS	Miyazawa et al., 2005
81	Daucosterol	C35H60O6	A. lappa, A. tomentosum	Seeds/fruits	UV, IR, 1H-NMR, 13C-NMR, DEPT, 2D-NMR and MS/HPLC	Wang and Yang, 1993; Ting-Guo et al., 2001; Han et al., 2013
	Fatty acids
82	Docosanoic acid	C22H44O2	A. tomentosum	Seeds	GCMS	Zong et al., 2013
83	Eicosanoic acid	C20H40O2	A. tomentosum	Seeds	GCMS	Zong et al., 2013
84	cis-13-eicosenoic acid	C20H38O2	A. tomentosum	Seeds	GCMS	Zong et al., 2013
85	Methyl palmitate	C17H34O2	A. lappa	–	IR/NMR/EI-MS	Miyazawa et al., 2005
86	Methyl linoleate	C19H34O2	A. lappa	–	IR/NMR/EI-MS	Miyazawa et al., 2005
87	Methyl linolenate	C19H32O2	A. lappa	Roots	IR/NMR/EI-MS/GCMS	Miyazawa et al., 2005; Kuo et al., 2012
88	Methyl stearate	C19H38O2	A. lappa	–	IR/NMR/EI-MS	Miyazawa et al., 2005
89	Methyl oleate	C19H36O2	A. lappa	Roots	IR/NMR/EI-MS/GCMS	Miyazawa et al., 2005; Kuo et al., 2012
90	Hexadecanoic acid	C16H32O2	A. lappa, A. tomentosum	Fruits/seeds	UV/TLC/IR/NMR/EIMS/GCMS	Miyazawa et al., 2005; Boldizsar et al., 2010; Zong et al., 2013
91	9-hexadecenoic acid	C16H30O2	A. tomentosum	Seeds	GCMS	Zong et al., 2013
92	Linoleic acid	C18H32O2	A. lappa	Roots	IR/NMR/EI-MS/GCMS	Miyazawa et al., 2005; Boldizsar et al., 2010; Kuo et al., 2012
93	Linolenic acid	C18H30O2	A. lappa	Fruits	IR/NMR/EI-MS; GCMS	Miyazawa et al., 2005
94	Stearic acid	C17H35CO2H	A. lappa	Fruits	IR/NMR/EI-MS	Miyazawa et al., 2005
95	9,12-octadecadienoic acid	C18H32O2	A. tomentosum	Seeds	GCMS	Zong et al., 2013
96	Oleic acid	C18H34O2	A. lappa	Fruits	UV/IR/HPLC/NMR/EI-MS	Miyazawa et al., 2005; Boldizsar et al., 2010
97	Oxiraneoctanoic acid	C19H36O3	A. tomentosum	Seeds	GCMS	Zong et al., 2013
98	Tetracosanoic acid	C24H48O2	A. tomentosum	Seeds	GCMS	Zong et al., 2013
	Acetylenic compounds
99	Arctinone-a	C13H10O2S2	A. lappa	Roots	UV/TLC/IR/NMR/MS	Washino et al., 1986
100	Arctinone-b	C13H10OS2	A. lappa	Roots	UV/TLC/IR/NMR/MS	Washino et al., 1986
101	Arctinol-a	C13H12O2S2	A. lappa	Roots	UV/TLC/IR/NMR/MS	Washino et al., 1986
102	Arctinol-b	C13H12O2S2	A. lappa	Roots	UV/TLC/IR/NMR/MS	Washino et al., 1986
103	Arctinal	C12H8OS2	A. lappa	Roots	UV/TLC/IR/NMR/MS	Washino et al., 1986
104	Arctic acid-b	C13H8O3S2	A. lappa	Roots	UV/TLC/IR/NMR/MS	Washino et al., 1986
105	Arctic acid-c	C13H10O3S2	A. lappa	Roots	UV/TLC/IR/NMR/MS	Washino et al., 1986
106	Methyl arctate-b	C14H10O3S2	A. lappa	Roots	UV/TLC/IR/NMR/MS	Washino et al., 1986
107	Arctinone-a acetate	C15H10O3S2	A. lappa	Roots	UV/TLC/IR/NMR/MS	Washino et al., 1986
108	Dehydrodihydrocostus lactone	C15H21O2	A. lappa	Roots	UV/TLC/IR/NMR/MS	Washino et al., 1986, 1987
109	Dehydrocostus lactone	C15H19O2	A. lappa	Roots	UV/TLC/IR/NMR/MS	Washino et al., 1986, 1987
110	Lappaphen-a	C27H26O4S	A. lappa	Roots	UV/TLC/IR/NMR/MS	Washino et al., 1986, 1987
111	Lappaphen-b	C27H26O4S	A. lappa	Roots	UV/TLC/IR/NMR/MS	Washino et al., 1986, 1987
	Carboxylic acids/Quinic acids and derivatives
112	Caffeic acid	C9H8O4	A. lappa	Seeds/leaves/roots	TLC/HPLC/UPLC/LC/MS/ HRESI-MS	Chen et al., 2004; Lin and Harnly, 2008; Lou et al., 2010a,b; Ferracane et al., 2010; Predes et al., 2011; Tang et al., 2014; Lou et al., 2016; Al-Shammaa et al., 2017
113	Caffeic acid 4-O-glucoside	C15H18O9	A. lappa	Roots	LC-DAD-ESI/MS	Lin and Harnly, 2008; Lou et al., 2016
114	Chlorogenic acid	C16H18O9	A. lappa	Seeds/leaves/roots	TLC/HPTLC/HPLC/UPLC/LC/MS/MALDI-QIT-TOF MS/ HRESI-MS	Wang et al., 2001; Chen et al., 2004; Lin and Harnly, 2008; Ferracane et al., 2010; Lou et al., 2010a,b; Predes et al., 2011; Liu et al., 2012; Haghi et al., 2013; Qin et al., 2014; Liu et al., 2015; Lou et al., 2016; Al-Shammaa et al., 2017
115	p-coumaric acid	C9H8O3	A. lappa	Seeds/leaves/roots	UPLC/EIMS	Lou et al., 2010a,b; Tang et al., 2014
116	Coumaroylquinic acid	C16H18O8	A. lappa	Roots	HPTLC/LC/ESI–MS/MS	Rajasekharan et al., 2015
117	Benzoic Acid	C7H6O2	A. lappa	Leaves	UPLC/EIMS	Lou et al., 2010a,b
118	Cynarin	C25H24O12	A. lappa	Seeds/leaves/roots	UPLC/LC/MS	Ferracane et al., 2010; Lou et al., 2010a, 2016; Tang et al., 2014
119	Caffeoyl-hexose-hydroxyphenol	C21 H21O10	A. lappa	Roots	HPTLC/LC/ESI–MS/MS	Rajasekharan et al., 2015
120	1-O-caffeoylquinic acid	C16H18O9	A. lappa	Roots	GCMS/LC-DAD-ESI/MS	Lin and Harnly, 2008; Tousch et al., 2014
121	3-O-caffeoylquinic acid	C16H18O9	A. lappa	Roots	GCMS/LC-DAD-ESI/MS	Lin and Harnly, 2008; Tousch et al., 2014
122	4-O-caffeoylquinic acid	C16H18O9	A. lappa	Roots	GCMS/LC-DAD-ESI/MS	Lin and Harnly, 2008; Tousch et al., 2014
123	5-O-caffeoylquinic acid	C16H18O9	A. lappa	Roots	GCMS/LC-DAD-ESI/MS	Lin and Harnly, 2008; Jaiswal and Kuhnert, 2011; Tousch et al., 2014
124	1-O-,5-O-dicaffeoylquinic acid	C25H24O12	A. lappa	Roots	HPLC/NMR/MS	Maruta et al., 1995; Wang et al., 2001; Han et al., 2013; Rajasekharan et al., 2015
125	1-O-, 5-O-dicaffeoyl-3-O-succinylquinaiccid	C35 H40 015	A. lappa	Roots	NMR/EI-MS	Maruta et al., 1995
126	1-O,-5-O-dicaffeoyl-4-O-succinylquinic acid	C29H35015	A. lappa	Roots	NMR/MS	Maruta et al., 1995
127	1-O-,5-O-dicaffeoyl-3-O-	C33H39018	A. lappa	Roots	NMR/MS	Maruta et al., 1995
128	4-O-disuccinylquaicniidc and 1-O-,3-0-,5-O-tricaffeoyl-4-O-succinylquinic acid	C38H41018	A. lappa	Roots	NMR/MS	Maruta et al., 1995
129	1,3-di-O-caffeoylquinic acid	C25H24O12	A. lappa	Seeds/roots	LCMS/ MALDI-QIT-TOF MS	Lin and Harnly, 2008; Liu et al., 2012
130	1,5-di-O-caffeoylquinic acid	C25H24O12	A. lappa	Leaves/Seeds/roots	UPLC/HPLC/PDA/LCMS/ MALDI-QIT-TOF MS	Maruta et al., 1995; Lin and Harnly, 2008; Liu et al., 2012; Haghi et al., 2013; Tousch et al., 2014
131	1,5-di-O-caffeoyl-4-O-maloylquinic acid	C29H27O16	A. lappa	Roots	LCMS/ MALDI-QIT-TOF MS	Jaiswal and Kuhnert, 2011; Liu et al., 2012; Tousch et al., 2014
132	1,5-di-O-caffeoyl-3-O-maloylquinic acid	C25H27O16	A. lappa	Roots	LCMS/ MALDI-QIT-TOF MS	Jaiswal and Kuhnert, 2011; Liu et al., 2012; Tousch et al., 2014
133	1,5-di-O-caffeoyl-3-O-succinoylquinic acid	C29H27O15	A. lappa	Roots	LCMS/ MALDI-QIT-TOF MS	Maruta et al., 1995; Jaiswal and Kuhnert, 2011; Liu et al., 2012; Tousch et al., 2014
134	1,5-di-O-caffeoyl-3,4-di-O-succinoylquinic acid	C33H31O18	A. lappa	Roots	LCMS/ MALDI-QIT-TOF MS	Liu et al., 2012; Tousch et al., 2014
135	1,3,5-tri-O-caffeoyl-4-O-succinoylquinic acid	C38H33O18	A. lappa	Roots	GCMS/LCMS/ MALDI-QIT-TOF MS	Jaiswal and Kuhnert, 2011; Liu et al., 2012; Tousch et al., 2014
136	1,3,5-tri-O-caffeoylquinic acid	C34H29O15	A. lappa	Roots	GCMS/LCMS/ MALDI-QIT-TOF MS	Liu et al., 2012; Tousch et al., 2014
137	1,5-di-O-caffeoyl-3-O-succinoyl-4-O-maloyquinic acid	–	A. lappa	Roots	LCMS/ MALDI-QIT-TOF MS	Liu et al., 2012
138	5-sinapoylquinic acid	C18H22O10	A. lappa	Roots	LC-DAD-ESI/MS	Lin and Harnly, 2008
139	3-sinapoyl-5-caffeoylquinic acid	C27H28O13	A. lappa	Roots	LC-DAD-ESI/MS	Lin and Harnly, 2008
140	3-sinapoyl-5-caffeoyl-1-methoxyoxaloylquinic acid	–	A. lappa	Roots	LC-DAD-ESI/MS	Lin and Harnly, 2008
141	4-sinapoyl-5-caffeoyl-1-methoxyoxaloylquinic acid	–	A. lappa	Roots	LC-DAD-ESI/MS	Lin and Harnly, 2008
142	3,4-dicaffeoylquinic acid	C25H24O12	A. lappa	Roots/seeds	LC-DAD-ESI/MS	Lin and Harnly, 2008
143	1,4-di-O-caffeoylquinic acid	C25H23O12	A. lappa	Roots	GCMS/LC-DAD-ESI/MS	Lin and Harnly, 2008; Jaiswal and Kuhnert, 2011; Tousch et al., 2014
144	3,5-di-O-caffeoylquinic acid	C25H24O12	A. lappa	Roots	GCMS/LC-DAD-ESI/MS	Lin and Harnly, 2008; Jaiswal and Kuhnert, 2011; Tousch et al., 2014
145	4,5-dicaffeoylquinic acid	C25H24O12	A. lappa	Roots/seeds	LC-DAD-ESI/MS	Lin and Harnly, 2008
146	3,5-dicaffeoyl-1-methoxyoxaloylquinic acid	–	A. lappa	Roots	LC-DAD-ESI/MS	Lin and Harnly, 2008
147	3-feruloyl-5-caffeoylquinic acid	–	A. lappa	Roots	LC-DAD-ESI/MS	Lin and Harnly, 2008
148	4,5-dicaffeoyl-1-methoxyoxaloylquinic acid	–	A. lappa	Roots	LC-DAD-ESI/MS	Lin and Harnly, 2008
149	3-sinapoyl-5-caffeoyl-4-methoxyoxaloylquinic acid	–	A. lappa	Roots	LC-DAD-ESI/MS	Lin and Harnly, 2008
150	1,4,5-tricaffeoylquinic acid	C34H30O15	A. lappa	Roots	LC-DAD-ESI/MS	Lin and Harnly, 2008
151	3,4,5-tricaffeoylquinic acid	–	A. lappa	Roots	LC-DAD-ESI/MS	Lin and Harnly, 2008
152	1,4,5-tricaffeoyl-3-methoxyoxaloylquinic acid	–	A. lappa	Roots	LC-DAD-ESI/MS	Lin and Harnly, 2008
153	3-succinoyl-4,5-dicaffeoyl	–	A. lappa	Roots	LCMS	Jaiswal and Kuhnert, 2011
154	1,5-dicaffeoyl-3-succinoylquinic acid	–	A. lappa	Roots	HPLC/LCMS	Wang et al., 2001; Jaiswal and Kuhnert, 2011
155	1,5-di-O-caffeoyl-4-O-succinoylquinic acid	C29H27O15	A. lappa	Roots	GCMS/LCMS	Maruta et al., 1995; Jaiswal and Kuhnert, 2011; Liu et al., 2012; Tousch et al., 2014
156	3,4-dicaffeoyl-5-succinoylquinic acid	C29H28O15	A. lappa	Roots	LCMS	Jaiswal and Kuhnert, 2011
157	1,3-dicaffeoyl-5-fumaroylquinic acid	–	A. lappa	Roots	LCMS	Jaiswal and Kuhnert, 2011
158	1,5-dicaffeoyl-4-fumaroylquinic acid	–	A. lappa	Roots	LCMS	Jaiswal and Kuhnert, 2011
159	1,5-dicaffeoyl-3-maloylquinic acid	–	A. lappa	Roots	LCMS	Jaiswal and Kuhnert, 2011
160	1,4-di-O-caffeoyl-3-O-maloylquinic Acid	C29H27O16	A. lappa	Roots	LCMS	Jaiswal and Kuhnert, 2011
161	1,3-di-O-caffeoyl-4,5-di-O-maloylquinic	C33 H31 O20	A. lappa	Roots	GCMS/LCMS	Jaiswal and Kuhnert, 2011; Tousch et al., 2014
162	1,5-dicaffeoyl-4-maloylquinic acid	–	A. lappa	Roots	LCMS	Jaiswal and Kuhnert, 2011
163	1,4-di-O-maloyl-3,5-di-O-caffeoylquinic acid	C31H33O20	A. lappa	Roots	GCMS/LCMS	Jaiswal and Kuhnert, 2011; Tousch et al., 2014
164	1,3,5-tricaffeoyl-4-succinoylquinic acid	–	A. lappa	Roots	LCMS	Jaiswal and Kuhnert, 2011
165	1,5-dicaffeoyl-3,4-disuccinoylquinic acid	–	A. lappa	Roots	LCMS	Jaiswal and Kuhnert, 2011
166	1,5-dicaffeoyl-3-fumaroyl-4-succinoylquinic acid	–	A. lappa	Roots	LCMS	Jaiswal and Kuhnert, 2011
167	1-fumaroyl-3,5-dicaffeoyl-4-succinoylquinic acid	–	A. lappa	Roots	LCMS	Jaiswal and Kuhnert, 2011
168	1,5-di-O-caffeoyl-3-O-succinoyl-4-O-maloylquinic acid	C33H31O19	A. lappa	Roots	GCMS/LCMS	Jaiswal and Kuhnert, 2011; Tousch et al., 2014
169	Dimaloyl-dicaffeoylquinic acid isomer 1	C33H31O20	A. lappa	Roots	GCMS/LCMS	Tousch et al., 2014
170	Succinoyl-tricaffeoylquinic acid isomer	C38H33O18	A. lappa	Roots	GCMS/LCMS	Tousch et al., 2014
171	Maloyl-dicaffeoylquinic acid isomer	C29H27O15	A. lappa	Roots	GCMS/LCMS	Tousch et al., 2014
172	Dicaffeoyl-succinoyl-malonylquinic acid isomer 1	C33H31O19	A. lappa	Roots	GCMS/LCMS	Tousch et al., 2014
173	Dicaffeoyl-succinoyl-malonylquinic acid isomer 2	C33H31O20	A. lappa	Roots	GCMS/LCMS	Tousch et al., 2014
174	Dimaloyl-dicaffeoylquinic acid isomer 2	C33H31O20	A. lappa	Roots	GCMS/LCMS	Tousch et al., 2014
175	Dimaloyl-dicaffeoylquinic acid isomer 3	C33H31O20	A. lappa	Roots	GCMS/LCMS	Tousch et al., 2014
176	Maloyl-tricaffeoylquinic isomer	C28H32O19	A. lappa	Roots	GCMS/LCMS	Tousch et al., 2014
177	1,3,5-tri-O-caffeoyl-4-O-maloylquinic Acid	C38H33O19	A. lappa	Roots	GCMS/LCMS	Tousch et al., 2014
178	5-hydroxymaltol	C6H6O4	A. lappa	Roots	UV/IR/ESIMS/NMR	Han et al., 2013
179	Succinic acid	C4H6O4	A. lappa	Roots	UV/IR/ESIMS/NMR	Han et al., 2013
	Saccharides/Polysaccharides
180	Rhamnogalacturonan	C117H178O101	A. lappa, A. minus	Roots/leaves	Chromatography/NMR/sugar analysis	Kato and Watanabe, 1993; Carlotto et al., 2016
181	Xylan	(C5H8O4)n	A. lappa, A. minus	Roots/leaves	Chromatography/NMR/sugar analysis	Kato and Watanabe, 1993
182	Arabinan	C9H13N3O5	A. lappa, A. minus	Roots/leaves	Chromatography/ NMR/sugar analysis	Kato and Watanabe, 1993; Carlotto et al., 2016
183	Arabinogalactan	C20H36O14	A. lappa, A. minus	Roots/leaves	Chromatography/ NMR/sugar analysis	Kato and Watanabe, 1993; Carlotto et al., 2016
184	Galactan	C18H32O16	A. lappa, A. minus	Roots/leaves	Chromatography/ NMR/sugar analysis	Kato and Watanabe, 1993
185	Cellulose	C64H124O30	A. lappa, A. minus	Roots/leaves	Chromatography/ NMR/sugar analysis	Kato and Watanabe, 1993
186	Xyloglucan	C51H86O42	A. lappa, A. minus	Roots/leaves	Chromatography/ NMR/sugar analysis	Kato and Watanabe, 1993
187	Galacturonic acid	C6H10O7	A. lappa	Roots/leaves	Chromatography/ NMR	Carlotto et al., 2016
188	Galacturonic acid	C6H10O7	A. lappa	Roots	Chromatography	Fuchigami et al., 1990
189	Galactose	C6H12O6	A. lappa	Roots/leaves/fruits	Chromatography/ NMR	Fuchigami et al., 1990; Kardosova et al., 2003; Boldizsar et al., 2010; Carlotto et al., 2016
190	Glucose	C6H12O6	A. lappa	Roots/leaves/fruits	UV/NMR/HPLC/GCMS	Kardosova et al., 2003; Boldizsar et al., 2010; Li et al., 2013; Carlotto et al., 2016
191	Mannose	C6H12O6	A. lappa	Roots/leaves	NMR	Carlotto et al., 2016
192	Sucrose	C12H22O11	A. lappa	Roots	UV/NMR/HPLC/GCMS	Boldizsar et al., 2010; Li et al., 2013
193	Raffinose	C18H32O16	A. lappa	Fruits	UV/NMR/HPLC/GCMS	Boldizsar et al., 2010
194	Rhamnose	C6H12O5	A. lappa	Roots/leaves/fruits	UV/NMR/HPLC/GCMS	Boldizsar et al., 2010; Carlotto et al., 2016
195	Arabinose	C5H10O5	A. lappa	Roots/leaves/fruits	UV/NMR/HPLC/GCMS	Fuchigami et al., 1990; Kardosova et al., 2003; Boldizsar et al., 2010; Carlotto et al., 2016
196	Inulin (fructan)	(C6H10O5)n	A. lappa, A. tomentosum	Roots	HPTLC/MS/NMR/HPLC-ELSD	Kardosova et al., 2003; Turdumambetov et al., 2004; Milani et al., 2011; Olennikov and Tankhaeva, 2011; Li et al., 2013; Liu et al., 2014
197	Fructose	C6H12O6	A. lappa	Roots	HPLC-ELSD	Li et al., 2013
198	Sorbitol	C6H14O6	A. lappa	Fruits	UV/NMR/HPLC/GCMS	Boldizsar et al., 2010
199	Mannitol	C6H14O6	A. lappa	Fruits	UV/NMR/HPLC/GCMS	Boldizsar et al., 2010
	Others
200	Crocin	C44H64O24	A. lappa	Leaves	UPLC	Lou et al., 2016
201	b-asparagine	C4H8N2O3	A. lappa, A. tomentosum	Roots	IR/NMR	Boev, 2005

FIGURE 2.

Chemical structures of several relevant components present in Arctium species.

Lignans

Major biologically active lignans include mainly arctigenin (a dietary phytoestrogen) and its glycoside, arctiin (lignanolides) occurring commonly in seeds, roots, fruits, and leaves of A. lappa and A. tomentosum (Yu et al., 2003; Ming et al., 2004; Liu et al., 2005, 2012, 2015; Wang et al., 2005; Matsumoto et al., 2006; Gao et al., 2008; Boldizsar et al., 2010; Ferracane et al., 2010; Zhou et al., 2011; Qin et al., 2014; Su et al., 2015; Lou et al., 2016). In addition, seeds and roots are distributed with low levels of dilignans and sesquilignans. For the first time, two new sesquilignans, namely lappaol A and B were isolated and characterized from A. lappa seeds (Ichihara et al., 1976). Later, 3 more sesquilignans, namely, lappaol C, D, and E, and two dilignans, namely lappaol F and H, were structurally determined from the seeds of A. lappa (Ichihara et al., 1977, 1978; Yong et al., 2007; Su et al., 2015). Lappaol A, C, and F are also found in the fruits of A. tomentosum (Kardosova et al., 2003). Two new lignans, neoarctin A and B, along with other recognized compounds including arctiin, arctigenin, daucosterol, lappaol F, isolappaol C and matairesinol were identified in seeds of A. lappa (Wang and Yang, 1995; Kardosova et al., 2003; Yong et al., 2007; Gao et al., 2008; Qin et al., 2014; Su et al., 2015). A simple RP-HPLC method was developed to identify the presence of arctiin in fruits of A. lappa (Yu et al., 2003; Boldizsar et al., 2010). Using bioactivity-guided fractionation, lappaol A, C and F, arctiin and arctignan E were isolated and characterized from the ethanolic extract (95%) of A. lappa seeds (Ming et al., 2004). Likewise, HPLC/UPLC/LC/MS/MS methods have been developed to identify arctigenin and arctiin in the seeds, leaves and roots of A. lappa (Yu et al., 2003; Liu et al., 2005; Ferracane et al., 2010; Lou et al., 2010a,b; Predes et al., 2011). Further, a supercritical fluid extraction procedure was found to be superior for extracting arctiin from A. lappa fruits (Carlotto et al., 2016). A high-speed counter-current chromatography was employed to obtain the pure compound arctiin from the fruit extracts of A. lappa. Authors obtained 49% of arctiin identified based on LC-MS and NMR techniques (Wang et al., 2005). A novel butyrolactone lignan compound named diarctigenin was found to occur in the methanolic seed extracts of A. lappa (Han et al., 1994). The fruits of A. lappa are reported to contain a total of 13 compounds including 5 new natural products (Umehara et al., 1993). Among them, 6 compounds were identified as arctignan A-E and artctigenin. Later, the occurrence of arctigenin and arctiin was also established from the leaves and seeds of A. lappa (Umehara et al., 1993; Liu et al., 2005; Matsumoto et al., 2006). Besides, the active extract resulted from the bioassay-guided fractionation of seed methanolic extract contained five active compounds including a new sesquilignan named isolappaol C and four known sesquilignan and dilignans namely, diarctigenin, and lappaol C, D, and F (Ferracane et al., 2010). Further, improved methods of extraction and analysis revealed that the seeds and roots of A. lappa contain arctigenin, arctiin, arctignan E, matareisinol, lappaol A, C, and F (Ferracane et al., 2010; Lou et al., 2010a, 2016; Liu et al., 2015; Su et al., 2015). The occurrence of 8 lignans in seeds and 1 lignan in roots of A. lappa was determined. The identified lignans were arctiin, arctigenin arctignan D and E, lappaol A, C, and H, isolappaol C and matairesinol (Liu et al., 2012; Haghi et al., 2013). Likewise, syringaresinol was reported in the chloroform fraction of A. lappa roots (Han et al., 2013). A rare butyrolactone lignan named arctiidilactone, and 11 novel butyrolactone lignans [arctigenin-4-O-β-D-gentiobioside, arctigenin-4-O-α-D-galactopyranosyl-(1→6)-O-β-D-glucopyranoside, arctigenin-4-O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside, 5′-propanediolmatairesinoside, (7′R,8R,8′R)-rafanotrachelogenin-4-O-β-D-glucopyranoside, (7′S,8R,8′R)-rafanotrachelogenin-4-O-β-D-glucopyranoside, (7S,8S,8′R)-4,7-dihydroxy-3,3′, 4-trimethoxyl-9-oxo benzylbutyrolactone lignan-4-O-β-D-glucopyranoside, (7R,8S,8′R) -4,7,4′-trihydroxy-3,3′-dimethoxyl-9-oxo dibenzylbutyrolactone lignan-4-O-β-D-glucopyranoside, (7S,8S,8′R)-4,7-dihydroxy-3,3′,4′-trimethoxyl-9-oxo dibenzylbutyrolactone lignan, arctiidilactone, arctiiapolignan A and arctiisesquineolignan A] were determined in A. lappa fruits (Yang et al., 2015). Phylligenin, matairesinoside and pinoresinol were reported only in the fruits of A. lappa (Boldizsar et al., 2010). Also, 2 secolignans, styraxlignolide D and styraxlignolide E (Yang et al., 2015) and 4 new neolignan glucosides namely, (8R)-4,9,9′-trihydroxy-3,3′-dimethoxy-7-oxo-8-O-4′-neolignan-4-O-β-D-glucopyranoside, (7S,8R)-4,7,9,9′-tetrahydroxy-3,3′-dimethoxy-8-O-4′-neolignan-9′-O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside, (7′S,8′R,8S)-4,4′, 9′-trihydroxy-3,3′-dimethoxy -7′,9-epoxylignan-7-oxo-4-O-β-D-glucopyranoside and (7R, 8S)-dihydrodehydrodiconiferyl alcohol-7′-oxo-4-O-β-D-glucopyranoside are reported from A. lappa fruits (Huang X.Y. et al., 2015). Besides, phytochemical analysis of A. lappa fruits revealed the existence of 2 more lignans named arctiisesquineolignan B and arctiiphenolglycoside A (He et al., 2016). Bioassay-guided separation and purification of hydroethanolic extracts of A. lappa fruits allowed to identify a new lignan, (+)-7,8-didehydroarctigenin along with arctigenin and matairesinol identified previously (Matsumoto et al., 2006).

Fatty Acids and Esters

In search of α-glucosidase inhibitory compounds, Miyazawa et al. (2005) found 11 compounds in A. lappa methanol extract. Among them, 10 compounds belonged to fatty acids. The identified compounds were linolenic acid, linoleic acid, methyl linoleate, methyl oleate, methyl linolenate, oleic acid, palmitic acid, methyl palmitate, methyl stearate, and stearic acid. Methanol extract from Arctium lappa L. which was found to contain sitosterol-β-D-glucopyranoside, methyl palmitate, methyl linoleate and methyl linoleneate showed an inhibitory activity against α-glucosidase at 97.3, 73.4, 66.5, and 68.5% respectively at a concentration of 200.0 μM (Miyazawa et al., 2005). Later, Kuo et al. (2012) identified methyl methyl α-linolenate, linolenic acid and methyl oleate as the chief constituents in the n-hexane fraction of A. lappa root (Kuo et al., 2012). The presence of linoleic acid, oleic acid, palmitic acid and stearic acid were also reported from A. lappa fruits (Boldizsar et al., 2010). Fatty acid composition of A. tomentosum seeds showed the occurrence of docosanoic acid, hexadecanoic acid, 9-hexadecenoic acid, 9,12-octadecadienoic acid, oxiraneoctanoic acid, eicosanoic acid, cis-13-eicosenoic acid, and tetracosanoic acid (Zhou et al., 2011).

Acetylenic Compounds

From the roots of A. lappa, Washino et al. (1986) isolated and characterized 9 sulfur-containing acetylenic compounds namely, arctinone-a, arctinone-b, arctinol-a, arctinol-b, arctinal, arctic acid-b, arctic acid-c, methyl arctate-b, and arctinone-a acetate. Based on the chemical and spectral analysis, it was found that all these compounds were derivatives of 5′- (1-propynyl)-2,2′-bithienyl-5-yl. Later, the occurrence of few guaianolides linked with a sulfur-containing acetylenic compounds namely dehydrodihydrocostus lactone, dehydrocostus lactone, lappaphen-a and lappaphen-b were discovered from the acetone extracts of A. lappa roots (Washino et al., 1986, 1987). Several bioactivities of the key A. lappa constituents have been well-described in literature including antibacterial and antifungal activities of acetylenic compounds (Takasugi et al., 1987) and anti-edematogenic activity on carrageenan-induced paw edema (Carlotto et al., 2016).

Phytosterols

Daucosterol, a natural phytosterol-like compound, was obtained from the seeds of A. lappa (Ahangarpour et al., 2017). The fruits of A. tomentosum are reported to contain 2 steroids, such as daucosterol and β-sitosterol. Using bioactivity-guided fractionation, daucosterol and β-sitosterol were recovered from the ethanolic extract (95%) of A. lappa seeds (Ming et al., 2004). Later, sitosterol-beta-D-glucopyranoside was found in the methanolic extracts of A. lappa (Miyazawa et al., 2005). Also, daucosterol and β-sitosterol compounds were detected from the chloroform extracts of A. lappa roots (Han et al., 2013). It was shown that phytosterol daucosterol inhibited cancer cell proliferation by inducing autophagy through reactive oxygen species-dependent manner (Zhao et al., 2015), and exhibited immunoregulatory activity by inducing protective Th1 immune response (Lee et al., 2007).

Polysaccharides

For the first time, Fuchigami et al. (1990; Ferracane et al., 2010) determined the pectic polysaccharides in edible A. lappa roots. Later investigations revealed the occurrence of several kinds of polysaccharides such as pectic substance, rhamnogalacturonan with neutral sugars, hemicellulose (arabinan, arabinogalactan, galactan, xylan, and xyloglucan), galacturonic acid, glucose, galactose, arabinose, rhamnose, mannose, and cellulose in cell walls of A. lappa and A. minus roots and leaves (Kato and Watanabe, 1993; Carlotto et al., 2016). Also, arabinose, glucose, galactose, rhamnose, and raffinose are reported from fruits of A. lappa (Boldizsar et al., 2010). The xyloglucan characterized from A. minus comprised a repeated unit of oligosaccharides of hepta-(Glc–Xyl = 4:3), deca-(Glc–Xyl–Gal–Fuc = 4:3:2:1) and nona-(Glc–Xyl–Gal–Fuc = 4:3:1:1) saccharides in the ratio of 14:5:12 (Kato and Watanabe, 1993). Biologically active inulin-type fructofuranans and other fructooligosaccharides have been identified from the roots of A. lappa (Kardosova et al., 2003). Inulin, a fiber comprising oligomers and polymers of fructose units linked by β(2→1) fructosyl–fructose bonds, has also been reported in the roots of A. lappa (Rajasekharan et al., 2015). A water-soluble polysaccharide fructan with a molecular weight of 4,600 Da, named as ALP1, was purified from A. lappa root and was composed of fructose and glucose in the molar ratio of 13:1. They were linked in →(1)-Fruf-(2)→, Fruf-(2)→ and Glcp-(1)→ (Liu et al., 2014). The structure was similar to the crude fructan obtained previously by Kardosova et al. (2003). In A. tomentosum, the glucofructans content is 24%, constituted by a polymer of 2 inulin type (GF-A and GF-B) and 1 graminan (a mixed type of glucofructans containing 1,2- and 2-6 bonds) type polysaccharides. HPTLC method was developed by Olennikov and Tankhaeva (2011) to quantify fructans in A. tomentosum and A. lappa (Olennikov and Tankhaeva, 2011). Two sugar alcohols, mannitol and sorbitol were reported from the fruits of A. lappa (Boldizsar et al., 2010). The yield of inulin from A. lappa root was successfully increased by adopting an ultrasonic extraction technology (Milani et al., 2011). It was indicated that water-soluble polysaccharide from A. lappa could significantly ameliorate the dysregulation of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) and anti-inflammatory cytokine (IL-10) caused by colitis (Wang et al., 2019).

Caffeoylquinic Acid Derivatives (Carboxylic Acids)

Caffeoylquinic acids are the major bioactive phenolic compounds of Arctium species and impart superior antioxidant properties to the plant. The roots of A. lappa were reported to contain caffeoylquinic acid derivatives such as 1-0-,5-O-dicaffeoylquinic acid, 1-0-,5-O-dicaffeoyl-3-O-succinylquinic acid, 1-0,-5-O-dicaffeoyl-4-O-succinylquinic acid, 1-0-,5-O-dicaffeoyl-3-O-,4-O-disuccinylquic acid and 1-0-,3-0-,5-O-tricaffeoyl-4-O- succinylquinic acid (Maruta et al., 1995). Chlorogenic acid content is much higher than the caffeic acid and both occur mainly in the skin of A. lappa roots (Chen et al., 2004). HPTLC analysis was used as a chemical profiling tool to estimate chlorogenic acid in A. lappa roots. The content ranged from 0.107 to 0.140%. Lin and Harnly (2008) and Liu et al. (2012) identified several compounds, including 5-sinapoylquinic acid, 3-sinapoyl-5-caffeoylquinic acid, 3-sinapoyl-5-caffeoyl-1- methoxyoxaloylquinic acid, 4-sinapoyl-5-caffeoyl-1-methoxyoxaloylquinic acid, 1,4-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 3,5-dicaffeoyl-1-methoxyoxaloylquinic acid, 3-feruloyl- 5-caffeoylquinic acid, 4,5-dicaffeoyl-1-methoxyoxaloylquinic acid, 3,5-dicaffeoyl-1- methoxyoxaloylquinic acid, 3-feruloyl-5-caffeoylquinic acid, 4,5-dicaffeoyl-1- methoxyoxaloylquinic acid, 3,4,5-tricaffeoylquinic acid, 1,4,5-tricaffeoylquinic acid and 1,4,5-tricaffeoyl-3-methoxyoxaloylquinic acid from the roots of A. lappa. Jaric et al. (2007) and Jaiswal and Kuhnert (2011) have characterized succinic, fumaric and malic acid-containing chlorogenic acid from the roots of A. lappa. These compounds included 3-succinoyl-4,5-dicaffeoyl, 1,5-dicaffeoyl-4-succinoylquinic acid, 1,5-dicaffeoyl-3-succinoylquinic acid, 3,4-dicaffeoyl-5-succinoylquinic acid, 1,5-dicaffeoyl-4-fumaroylquinic acid, 1,3-dicaffeoyl-5-fumaroylquinic acid, 1,4-dicaffeoyl-3-maloylquinic acid, 1,5-dicaffeoyl-3-maloylquinic acid and 1,5-dicaffeoyl-4-maloylquinic acid, 1,3,5-tricaffeoyl-4-succinoylquinic acid, 1,5-dicaffeoyl-3,4-disuccinoylquinic acid, 1,5-dicaffeoyl-3-fumaroyl-4-succinoylquinic acid, 1-fumaroyl-3,5-dicaffeoyl-4-succinoylquinic acid, 1,5-dicaffeoyl-3-succinoyl- 4-dimaloylquinic acid and dicaffeoyldimaloylquinic acid. Further, Liu et al. (2012) isolated and identified 12 caffeoylquinic acids in both seeds and roots of A. lappa. The identified compounds included chlorogenic acid, 1,5-di-O-caffeoylquinic acid, 1,3-di-O-caffeoylquinic acid, dicaffeoyl-maloylquinic acid, dicaffeoyl-maloylquinic acid, 1,3-di-O-caffeoylquinic acid, 1,5-di-O-caffeoyl-3-O-maloylquinic acid, 1,5-di-O-caffeoyl-3-O-succinoylquinic acid, 1,5-di-O-caffeoyl-4-O-maloylquinic acid, dicaffeoyl-dimaloylquinic acid, 1,5-di-O-caffeoylquinic acid, 1,5-di-O-caffeoyl-3-O-succinoyl-4-Omaloyquinic acid, 1,5-di-O-caffeoyl-3,4-di-O-succinoylquinic acid, and 1,3,5-tri-O-caffeoyl-4-O-succinoylquinic acid. In addition, phytochemical analysis of root extracts of A. lappa showed the occurrence of 8 additional isomers of hydroxycinnamic acids (Liu et al., 2012; Tousch et al., 2014). An average content of chlorogenic acid, 1-O-5-O-dicaffeoylquinic acid and 1,5-dicaffeoyl- 3-succinylquinic acid was observed to be between 1.7 and 7.9 mg/g dry weight of roots (Wang et al., 2001). Two new neolignan glucosides named (70S, 80R, 8S)-4,40,90- trihydroxy-3,30-dimethoxy-70,9-epoxylignan-7-oxo-4-O-b-D-glucopyranosyl-40-O-b-D-glucopyranoside and (7S, 8R)-4,7,9,90-tetrahydroxy-3,30-dimethoxyl- 70-oxo-8-40- oxyneolignan-4-O-b-D-glucopyranoside were determined from the fruit extract of A. lappa (Yang et al., 2012). The occurrence of phenolic acids, caffeic acid, cynarin and chlorogenic acid has been reported for the first time in A. lappa seeds and leaves (Ferracane et al., 2010; Tang et al., 2014; Lou et al., 2016) and later in both seeds and roots (Predes et al., 2011). Chlorogenic acid was determined by Tardio et al. (2005) in the seeds of A. lappa. Further, UPLC analysis revealed the presence of caffeic acid, benzoic acid and p-coumaric acid in the leaves of A. lappa (Tardio et al., 2005; Lou et al., 2010a,b). From the ethyl acetate and n-butanol fractions of A. lappa, 1,5-O-two caffeoylquinic acids, succinic acid and 5-hydroxy maltol were identified for the first time (Han et al., 2013). Likewise, HPLC and UPLC with photodiode array (PDA) detector were used to quantify caffeoyl esters, chlorogenic acid and 1,5-dicaffeoylquinic acid in aerial parts and root samples of A. lappa (Haghi et al., 2013). Two more phenolic compounds, namely, coumaroylquinic acid and caffeoyl-hexose-hydroxyphenol were identified by Rajasekharan et al. (2015) in the root extracts of A. lappa (Rajasekharan et al., 2015). It was reported that caffeoylquinic acids and their derivatives show multiple pharmacological activities including decrease in diet-induced obesity via modulation of PPARα and LXRα transcription (Huang K. et al., 2015) and anti-ulcerogenic effect (Lee et al., 2010).

Flavonoids

The reported flavonoids include flavonols, flavones, and their glycosides. Two major constituents, namely rutin and isoquercetin, along with few other minor flavonoids including kaempferol-3-O-rhamnoglucoside, quercimeritrin and astragalin were identified in the ethanolic extracts of A. minus leaves (Saleh and Bohm, 1971). Likewise, the occurrence of quercetin-3-O-rhamnoside was reported from the leaves of A. lappa. Later, the presence of phenolic compounds such as quercetin, quercitrin, rutin, and luteolin have been reported in seeds, fruits, leaves and roots of A. lappa (Saleh and Bohm, 1971; Tamayo et al., 2000; Yu et al., 2003; Lou et al., 2010a,b, 2016; Predes et al., 2011; Liu et al., 2012; Tang et al., 2014). Also, few isoflavone derivatives including genistein, nobilein, biachanin A and tangeretin have been detected in A. lappa roots (Eberding et al., 2007). A comparative study has shown the existence of chemical differences within the A. lappa organs (Ferracane et al., 2010). According to them, luteolin and quercetin rhamnoside were detected in roots whereas rutin, quercetin, quercitrin and luteolin in leaves. On the other hand, no flavonoids were found in the seeds of A. lappa. Two more flavonols, namely quercetin 3-O-glucuronide and quercetin 3-vicianoside, were identified by Rajasekharan et al. (2015) in the root extracts of A. lappa.

Terpenoids

The fruits of A. lappa were found to contain β-eudesmol, a sesquiterpene alcohol (Rajasekharan et al., 2015; Yang et al., 2015). Pentacyclic triterpenoids, such as ursolic and oleanolic acids were detected by Han et al. in the ethanolic extract of A. lappa roots (Han et al., 2013). Arctiopicrin and onopordopicrin are the sesquiterpene lactones isolated from the leaf extract of A. lappa (Barbosa et al., 1993; Machado et al., 2012). Arctiopicrin occurrence is also evidenced in A. lappa. Later, few more sesquiterpene lactones, namely dehydromelitensin-8- (4′-hydroxymethacrylate), dehydromelitensin, and melitensin and a norisoprenoid along with 2 more terpenes such as dehydrovomifoliol and loliolide were identified in A. lappa leaf (Machado et al., 2012). Onopordopicrin, a germacranolide sesquiterpene lactone was isolated from the aerial parts of A. nemorosum (Zimmermann et al., 2012). Two triterpenoids, namely 3α-acetoxy-hop-22(29)-ene and 3α-hydroxylanosta-5, 15-diene were isolated from the leaves of A. lappa (Jaiswal and Kuhnert, 2011).

Others

From the concentrated sap obtained from A. lappa roots (A. lappa and A. tomentosum), β-asparagine was isolated for the first time (Boldizsar et al., 2010). The carotenoid crocin was reported to occur in the leaves of A. lappa (Lou et al., 2016).

Volatile Compounds

A total of 101 volatile chemical constituents were identified in A. lappa. The details of these compounds are partially summarized in Table 2 and described in this section. Carboxylic acids and fatty acids were more prevalent in A. lappa. On the other hand, there are no available literatures on the identification of volatile components in other Arctium species. The chemical structures of some compounds from Arctium species are shown in Figure 2.

Table 2.

Volatile constituents of Arctium spp.

S. No	Compound name	Formula	Species	Plant origin/part	Analytical method	References
	Hydrocarbons
1	Aplotaxene	C17H28	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
2	Clovene	C15H24	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
3	Dihydroaplotaxene	C17H30	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
4	Docosane	C22H46	A. lappa	Leaves	GCMS	Aboutabl et al., 2013
5	Eicosane	C20H42	A. lappa	Roots/leaves/seeds	GCMS	Aboutabl et al., 2013
6	1-Heptadecene	C17H34	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
7	Heptacosane	C27H56	A. lappa	Roots/leaves	GCMS	Aboutabl et al., 2013
8	Hexacosane	C26H54	A. lappa	Roots/leaves	GCMS	Aboutabl et al., 2013
9	Nonadecane	C19H40	A. lappa	Leaves	GCMS	Aboutabl et al., 2013
10	2-Naphthalenemethanol	C11H10O	A. lappa	Roots	GCMS	Wang et al., 2004
11	1-Pentadecene	C15H30	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
12	Pentacosane	C25H52	A. lappa	Roots	GCMS	Aboutabl et al., 2013
13	Pentadecane	C15H32	A. lappa	Roots/leaves	GCMS	Aboutabl et al., 2013
14	Tetracosane	C24H50	A. lappa	Roots/leaves	GCMS	Aboutabl et al., 2013
15	Tetradecane	C14H30	A. lappa	Leaves	GCMS	Aboutabl et al., 2013
	Aldehydes
16	Benzaldehyde	C7H6O	A. lappa	Roots	GCMS	Washino et al., 1986, 1987; Wang et al., 2004
17	Butanal	C4H8O	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
18	Decanal	C10H20O	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
19	Dodecanal	C12H24O	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
20	Heptanal	C7H14O	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
21	Hexanal	C6H12O	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
22	(Z)-3-Hexenal	C6H10O	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
23	(E)-2-Hexenal	C6H10O	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
24	2-Methylpropanal	C4H8O	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
25	3-Methylbutanal	C5H10O	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
26	Nonanal	C9H18O	A. lappa	Roots/leaves/seeds	GCMS	Washino et al., 1986, 1987
27	Octanal	C8H16O	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
28	(E)-2-Octanal	C8H14O	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
29	Phenylacetaldehyde	C8H8O	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
30	Pentanal	C5H10O	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
31	Propanal	C3H6O	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
32	Tridecanal	C13H26O	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
33	4-Methoxybenzaldehyde	C8H8O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
34	Undecanal	C11H22O	A. lappa	Roots		Washino et al., 1986, 1987
	Methoxypyrazines
35	2-Methoxy-3-methylpyrazine	C6H8N2O	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
36	2-Isopropyl- 3-methyoxylpyrazine	C8H12N2O	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
37	2-Methoxy-3- propylpyrazine	C8H12N2O	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
38	2-sec-Butyl-3-methoxypyrazine	C9H14N2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
39	2-Isobutyl-3-methoxypyrazine	C9H14N2O	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
40	2-Butyl-3- methoxypyrazine	C9H14N2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
41	2-Isoamyl-3-methoxypyrazine	C9H14N2O	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
	Fatty acids/Carboxylic acids
42	Acetic acid	CH3COOH	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
43	Benzoic acid	C7H6O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
44	Butyric acid	C4H8O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
45	Cinnamic acid	C9H8O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
46	Costic acid	C15H22O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
47	Decanoic acid	C10H20O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
48	Dodecanoic acid	C12H24O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
49	Ethyl oleate	C20H38O2	A. lappa	Seeds	GCMS	Aboutabl et al., 2013
50	Hexanoic acid	C3H6O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
51	Hexadecanoic acid	C17H34O2	A. lappa	Roots/seeds	GCMS	Washino et al., 1986, 1987; Aboutabl et al., 2013
52	(E)-3-Hexenoic acid	C6H10O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
53	Heptanoic acid	C7H14O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
54	(E)-3-Heptenoic acid	C7H12O2	A. lappa	Roots	GCMS	Washino et al., 1986;, 1987
55	Linoleic acid	C18H32O2	A. lappa	Roots	GCMS	Wang et al., 2004
56	2, 3-Hydroxyoctanoic acid	C8H16O3	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
57	2-Methylpropionic acid	C4H8O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
58	2-Methylbutyric acid	C5H10O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
59	3-Methoxybenzoic acid	C8H8O3	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
60	Methyl palmitate	C17H34O2	A. lappa	Roots/seeds	GCMS	Washino et al., 1986, 1987; Aboutabl et al., 2013
61	Methyl linolenate	C19H32O2	A. lappa	Roots	GCMS	Wang et al., 2004
62	Methyl oleate	C19H36O2	A. lappa	Seeds	GCMS	Aboutabl et al., 2013
63	Nonanoic acid	C9H18O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
64	Nonanedioic acid	C9H16O4	A. lappa	Roots	GCMS	Washino et al., 1986;, 1987
65	(E)-3-nonenoic acid	C9H16O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
66	Octanoic acid	C8H16O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
67	(E)-3-Octenoic acid	C8H14O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
68	Octadecanoic acid	C18H36O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
69	Octadecanoic acid methyl ester	C18H36O2	A. lappa	Seeds	GCMS	Aboutabl et al., 2013
70	Pentanoic acid	C5H10O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
71	Phenylacetic acid	C8H8O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
72	Phenylpropionic acid	C9H10O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
73	Propionic acid	C3H6O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
74	Pentadecanoic acid	C15H30O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
75	Salicylic acid	C7H6O3	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
76	Tridecanoic acid	C13H26O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
77	Tetradecanoic acid	C14H28O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
78	Undecanoic acid	C11H22O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
	Terpenes/terpenoids
	Monoterpenoids
79	Carvomenthone	C10H18O	A. lappa	Roots/leaves	GCMS	Aboutabl et al., 2013
80	Geraniol	C10H18O	A. lappa	Seeds	GCMS	Aboutabl et al., 2013
81	Linalool	C10H18O	A. lappa	Seeds	GCMS	Aboutabl et al., 2013
82	Thymol	C10H14O	A. lappa	Seeds	GCMS	Aboutabl et al., 2013
83	Z-citral	C10H16O	A. lappa	Seeds	GCMS	Aboutabl et al., 2013
84	E-citral	C10H16O	A. lappa	Seeds	GCMS	Aboutabl et al., 2013
	Sesquiterpenoids
85	Dehydrocostus lactone	C15H18O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
86	Dehydrodihydrocostus lactone	C15H29O2	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
	Oxygenated sesquiterpenes
87	Caryophyllene oxide	C15H24O	A. lappa	Roots/leaves	GCMS	Aboutabl et al., 2013
88	β-Costol	C15H24O	A. lappa	Roots	GCMS	Aboutabl et al., 2013
	Sesquiterpene Hydrocarbons
89	Aromadendrene	C15H24	-	Roots/seeds	GCMS	Aboutabl et al., 2013
90	Caryophyllene	C15H24	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
91	γ-Cadinene	C15H24	-	Roots/leaves/seeds	GCMS	Aboutabl et al., 2013
92	Cyperene	C15H24	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
93	β-Elemene	C15H24	A. lappa	Roots	GCMS	Washino et al., 1986, 1987; Aboutabl et al., 2013
94	trans-β-Farnesene	C15H24	A. lappa	Roots/leaves	GCMS	Aboutabl et al., 2013
95	α-Guaiene	C15H24	A. lappa	Roots	GCMS	Washino et al., 1986, 1987
96	Isoaromadendrene epoxide	C15H24O	–	Roots/leaves/seeds	GCMS	Aboutabl et al., 2013
97	Limonene	C10H16	A. lappa	Leaves/seeds	GCMS	Washino et al., 1986, 1987
98	α-Myrcene	C10H16	A. lappa	Seeds	GCMS	Aboutabl et al., 2013
99	α-Pinene	C10H16	A. lappa	Roots/leaves	GCMS	Aboutabl et al., 2013
100	Squalene	C30H50	A. lappa	Seeds	GCMS	Aboutabl et al., 2013
	Sesquiterpene Alcohol
101	β-Copaen-4α-ol	C15H24O	–	Roots/leaves/seeds	GCMS	Aboutabl et al., 2013

Hydrocarbons

Fourteen hydrocarbon compounds, aplotaxene, clovene, dihydroaplotaxene, eicosane, 1-heptadecene, heptacosane, hexacosane, nonadecane, 2-naphthalenemethanol, 1-pentadecene, pentacosane, pentadecane, tetracosane, and tetradecane were detected from the roots, seeds, and leaves of A. lappa (Washino et al., 1986; Wang et al., 2004). In addition, docosane, eicosane, heptacosane, hexacosane, tetracosane, and pentadecane were found only in roots and leaves. Docosane was found only in leaves, while seeds of A. lappa contained only eicosane.

Aldehydes

Nineteen aldehydes, namely, benzaldehyde, butanal, decanal, dodecanal, heptanal, hexanal, (Z)-3-hexenal, (E)-2-hexenal, 2-methylpropanal, 3-methylbutanal, nonanal, octanal, (E)-2-octanal, phenylacetaldehyde, pentanal, propanal, tridecanal, 4-methoxybenzaldehyde, and undecanal were found as root volatile compounds in A. lappa (Washino et al., 1986, 1987; Wang et al., 2004). Interestingly, only the alkyl aldehyde nonanal was present in all plant parts such as roots, leaves, and seeds (Washino et al., 1986, 1987).

Methoxypyrazines

Seven methoxypyrazines, such as 2-methoxy-3-methylpyrazine, 2-methoxy-3- propylpyrazine, 2-isopropyl- 3-methyoxylpyrazine, 2-sec-butyl-3-methoxypyrazine, 2-butyl-3- methoxypyrazine, 2-isobutyl-3-methoxypyrazine, and 2-isoamyl-3-methoxypyrazine were detected in roots of A. lappa (Washino et al., 1986, 1987).

Carboxylic Acids and Fatty Acids

Twenty-two carboxylic acids namely acetic acid, benzoic acid, butyric acid, cinnamic acid, costic acid, dodecanoic acid, hexanoic acid, (E)-3-hexenoic acid, heptanoic acid, (E)-3-heptenoic acid, 2, 3-hydroxyoctanoic acid, 2-methylpropionic acid, 2-methylbutyric acid, 3-methoxybenzoic acid, nonanoic acid, nonanedioic acid, pentanoic acid, phenylacetic acid, phenylpropionic acid, propionic acid, salicylic acid, and undecanoic acid were identified in A. lappa roots (Washino et al., 1986, 1987; Wang et al., 2004). Fatty acids such as decanoic acid, hexadecanoic acid, linoleic acid, octanoic acid, (E)-3-octenoic acid, octadecanoic acid, pentadecanoic acid, tridecanoic acid, and tetradecanoic acid were found in roots while ethyl oleate, methyl oleate, hexadecanoic acid, methyl palmitate, and octadecanoic acid methyl ester were identified in seeds of A. lappa (Washino et al., 1986, 1987; Wang et al., 2004).

Monoterpenes and Sesquiterpenes

Three alcoholic and one phenolic monoterpenoids (carvomenthone, geraniol, linalool, and thymol); 2 sesquiterpene lactones (dehydrocostus lactone and dehydrodihydrocostus lactone, isoaromadendrene epoxide); 2 oxygenated sesquiterpenes (caryophyllene oxide and β-costol) and 12 sesquiterpene hydrocarbons namely, aromadendrene, caryophyllene, γ-cadinene, cyperene, β-elemene, trans-β-farnesene, α-guaiene, limonene, myrcene, α-pinene, and squalene were identified in A. lappa (Washino et al., 1986, 1987; Wang et al., 2004). Geraniol, linalool, thymol, aromadendrene, γ-cadinene, isoaromadendrene epoxide, limonene, α-myrcene, and squalene were identified only in the seeds of A. lappa.α-Pinene, isoaromadendrene epoxide, γ-cadinene, carvomenthone, and caryophyllene oxide were found in the roots and leaves.

Bioactivities of Arctium Species

Arctium lappa is widely used as an ethno-medicinal plant especially in North America, Asia and Europe, and is applied to treat various diseases including diabetes, gout, rheumatism, and skin problems (Chan et al., 2011; Azizov et al., 2012). A. lappa roots have been used as a vegetable in Japanese (referred to as ‘gobo’) and Korean cuisine. Its root has been used to treat constipation, mercury poisoning, upper respiratory infections, inflammation and oxidative stress in patients with knee osteoarthritis (Maghsoumi-Norouzabad et al., 2016), while the leaves were efficacious in healing burns, rashes, and applied in women with labor condition (Force, 2001; Lewis and Elvin-Lewis, 2003; Amish Burn Study et al., 2014). A. lappa has also been found for the treatment of alopecia (loss of hair) among adults (Amish Burn Study et al., 2014). In Western countries, burdock is used as a remedy for several ailments ranging from arthritis, chronic inflammation, and various skin problems (e.g., scaly skin conditions such as psoriasis and eczema) to cancer treatment (Wu et al., 2010; Amish Burn Study et al., 2014).

Studies on the biological activities of extracts of different parts of A. lappa and compounds isolated thereof, were carried out and revealed antipyretic, antimicrobial, diuretic, diaphoretic, hypoglycaemic, antioxidant, anti-inflammatory, anti-hepatotoxicity, antiulcer, antimutagenicity, and antitumour activities.

Anticancer Effects

Arctium lappa fruit has been used in traditional medicine, and it is popular for its various anticancer effects. Arctigenin (ATG), a natural lignan product extracted from the seeds of Arctium lappa, has been shown to have estrogenic properties, that reduced the risk of osteoporosis, heart disease, and menopausal symptoms (Maxwell et al., 2017). It was found to possess antitumor effect by modulating the protein kinase activation pathway and hence rendering the tumor cells susceptible to effects of the nutrient-deprived environment (Awale et al., 2006). Later on, ATG was shown to induce apoptosis (programmed cell death) of estrogen receptor-negative cancer cells (MDA-MB-231) through the ROS/p38 MAPK pathway and epigenetic regulation of Bcl-2 by upregulating trimethylation of histone H3K9 (Hsieh et al., 2014). It was reported that ATG was able to inhibit cell proliferation and may induce apoptosis and cell cycle arrest at the G0/G1 phase in glioma cells (Maimaitili et al., 2017). In more detail, it was found that ATG increased the expression levels of p21, retinoblastoma and p53 proteins, and significantly decreased the expression levels of cyclin D1 and CDK4 proteins (Maimaitili et al., 2017). Furthermore, ATG was able to induce apoptosis in glioma cells, coupled with increased expression levels of cleaved caspase-3 and the pro-apoptotic BCL2-associated X protein (Maimaitili et al., 2017). ATG-induced apoptosis was significantly suppressed by the pretreatment of cells with Z-DEVD-FMK, a caspase-3 inhibitor (Maimaitili et al., 2017). More recently, study by Lou et al. (2017) demonstrated ATG to significantly inhibit in vitro migration and invasion of human breast cancer cells (MDA-MB-231) by downregulation of MMP-2, MMP-9 and heparanase (Lou et al., 2017).

Extracts from A. lappa also showed selective antiproliferative activity against certain human cancer cell lines including K562, MCF-7 and 786-0 (Predes et al., 2011). Lappaol F, a novel natural product isolated from the seeds of A. lappa, was found to suppress cancer cell growth in a dose-dependent manner in various human cancer cell lines through induction of G1 and G2 cell-cycle arrest. This effect was associated with strong induction of p21 and p27 and suppression of cyclin-dependent kinase 1 (CDK1) and cyclin B1 (Sun et al., 2014).

A. lappa is one of the herbs widely used by cancer patients in some Canadian populations to improve quality of life (QOL) and prevent cancer progression. A. lappa is one of the herbs constituting the two proprietary herbal products: Flor-Essence^® and Essiac^® suggested for prolong survival and the improvement of QOL among cancer patients (Tamayo et al., 2000).

Antidiabetic Effects

Root of A. lappa root has been found to mediate hypoglycemic activities making it a popular choice to be used as a traditional medicine in diabetes. Oral administration of burdock root ethanolic extract in streptozotocin-induced diabetic rats significantly lowered blood glucose and increased insulin level in the diabetic rats compared to the control diabetic group (Cao et al., 2012). Additionally, treatment with A. lappa extract also reduced the levels of serum total cholesterol (TC), triglycerides (TG) and low density lipoprotein (LDL), whereas high density lipoprotein (HDL) level was higher in the control rats. More recently in a similar study, Ahangarpour et al. (2017) investigated the antidiabetic and hypolipidemic properties of the root extract of A. lappa on nicotinamide-streptozotocin (NA-STZ)-induced type 2 diabetes in mice (Ahangarpour et al., 2017). The results show that root extract of A. lappa displays anti-diabetic effect at certain doses. It exerts its effects through hypolipidemic and insulinotropic properties and hence the root extract could serve successfully in treating patients with type 2 diabetes in the future. Moreover, sitosterol-β-D-glucopyranoside from burdock’s root acts as a potent inhibitor of alpha-glucosidases, thereby having the potential to reduce glycogenolysis and help to decrease blood glucose level (Tousch et al., 2014). In addition, Zhao and Zhou (2015) demonstrated that trace elements (e.g., Na, K, Mn, Fe, and Mg) present in the root and fruit extracts of A. lappa exhibit antidiabetic effects. While A. lappa constituents do reduce absorption of glucose, they also elevate inulin content in blood and slow digestion of carbohydrates to confer its anti-diabetic activities. The pharmacological mechanisms of A. lappa roots are slightly different from other classes of oral antihyperglycemic agents such as metformin. Metformin decreases hepatic glucose production, decreases intestinal absorption of glucose, and improves insulin sensitivity by increasing peripheral glucose uptake and utilization (Dumitrescu et al., 2015).

Anti-oxidant, Hepatoprotective and Gastroprotective Activities

It is believed that lignans and caffeoylquinic acids from A. lappa are of value because of their antioxidant capacity (Maruta et al., 1995; Mkrtchian et al., 1998; Jaiswal and Kuhnert, 2011) by which they can scavenge free radicals that are thought to play an important role in many diseases.

The hydroalcoholic extracts of burdock roots possess significant antioxidant potential as seen by the application of various assays. Very recently, Fierascu et al. (2018) quantified antioxidant potential of burdock extracts using DPPH (2,2-diphenyl-1-picrylhydrazyl) and phosphomolybdate assays to demonstrate that burdock extracts have very high antioxidative activities, presumably due to the high content of polyphenols (Fierascu et al., 2018). The potent antioxidative property makes these extracts effective inhibitors of lipid peroxidation in rat liver homogenate in vitro (Duh, 1998) and an excellent hepatoprotective agent in vivo and in vitro (Lin et al., 2000). Due to its radical scavenging ability, A. lappa is also used to treat gastrointestinal ulcers (da Silva et al., 2013).

Antimicrobial Effects

Extracts of different parts of A. lappa have been investigated for their microbial-modulatory properties by many researchers. An organic extract from A. lappa has shown inhibiting properties toward the growth of Pseudomonas aeruginosa, Escherichia coli, Lactobacillus acidophilus, Streptococcus mutans, and Candida albicans residing in the teeth of the oral cavity (Gentil et al., 2006). Pereira et al. (2005) further reported potent growth inhibiting activities of A. lappa extract against a broad spectrum of oral microorganisms, specifically those associated with teeth infections, namely Enterococcus faecalis, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, and Candida albicans (Pereira et al., 2005). Very recently, Fierascu et al. (2018) investigated antifungal potential of hydroalcoholic extract of burdock roots and observed that it is active against the fungal lines Aspergillus niger ATCC 15475 and Penicillium hirsutum ATCC 52323 (Fierascu et al., 2018). Fruit extract of A. lappa was tested (Dias et al., 2017) for in vitro antiviral properties against Herpes simplex virus type-1 (HSV-1) and was found to decrease viral load significantly at all concentrations tested (400, 50, and 3.125 μg/mL). At 400 μg/mL concentration, it showed comparable antiviral activity as acyclovir (50 μg/mL). Arctigenin, one of the key constituents of A. lappa extract, has shown potent activities against human immunodeficiency virus type-1 (HIV-1) both in vivo and in vitro presumably by increasing the expression of Heme oxygenase-2 (HO-2) and blocking HIV-1gag proteins (Schroder et al., 1990).

Anti-inflammatory Effects

Various parts of A. lappa demonstrated anti-inflammatory effects (Liu et al., 2005). Burdock extract is known to alleviate wound irritation and swelling and therefore has been traditionally used for healing burn wounds. This effect might be mediated through the inhibition of the cyclooxygenase-2 (COX-2) enzyme. Cyclooxygenase is a lipid metabolizing enzyme that catalyzes the oxygenation of polyunsaturated fatty acids. This process forms prostanoids, specifically eicosanoids, which are known to be potent cell signaling molecules connected to inflammatory processes (Charlier and Michaux, 2003). Phenolic compounds present in burdock extract (e.g., arctigenin, lappaol F, diarctigenin) are inhibitors of this enzyme (Zhao et al., 2009; Lee and Kim, 2010), thereby suppressing lipopolysaccharide (LPS)-stimulated NO production (Park et al., 2007) and pro-inflammatory cytokines secretion (including TNF-α and IL-6) in a dose-dependent manner (Zhao et al., 2009; Kwon et al., 2016). Arctigenin also strongly inhibited the expression of iNOS (Inducible Nitric Oxide Synthase) and its enzymatic activity (Wang et al., 2007; Zhao et al., 2009). Moreover, it induced endothelial nitric oxide synthase (eNOS) and supressed in a rat model subarachnoid hemorrhage-induced vasospasm by regulation of the PI3K/Akt signaling pathway (Chang et al., 2015). Among the studied phenolic compounds, diarctigenin was found to inhibit the DNA binding ability of NF-κB and to inhibit NF-κB-regulated iNOS expression (Kim et al., 2008), thereby overall targeting NF-κB-activating signaling cascade directly to confer anti-inflammatory response. Luteolin, an important flavonoid from burdock was also reported to possess significant anti-inflammatory properties (Ferracane et al., 2010; Nabavi et al., 2015).

Effects Against Skin Conditions

Leaves of Arctium species have been used in traditional medicinal practices in various skin conditions (e.g., rashes, boils, eczema, ichthyosis, acne, psoriasis, and abscesses) presumably due to the presence of various phenolic compounds. The potent antioxidant and anti-inflammatory properties of these compounds serve to detoxify and mediate healing action (Chan et al., 2011). Several hydroxycinnamic acids which are among the active phytochemicals in the A. lappa extracts (Liu et al., 2012; Tousch et al., 2014) have been found to act as free radical scavengers and possess antioxidant activities, which confer them potential to serve as skin protectors and wound healers (Graf, 1992; Phan et al., 2001; Taofiq et al., 2017). In addition, hydroxycinnamic acid derivatives also display anti-collagenase, anti-inflammatory, antimicrobial and anti-tyrosinase activities, as well as ultraviolet (UV) protective effects, suggesting that they can be exploited as anti-aging and anti-inflammatory agents, preservatives and hyperpigmentation-correcting ingredients (Ahangarpour et al., 2017). These bioactivities are the reason why burdock extracts find their use in various commercial cosmetic products.

Effect on Potency and Fertility

Diabetes mellitus induces many complications among which dysfunctions male reproductive system is worth mentioning. Glucose metabolism plays an important regulatory role on the production or development of mature spermatozoa (spermatogenesis) as well as on maintaining specific functions, such as motility and fertilization ability in mature sperm cells. Therefore, it is not surprising that A. lappa root extract, which has hypoglycemic and antioxidative properties, would have beneficial effects on male potency and fertility. Ahangarpour et al. (2015) investigated the effect of A. lappa root extract on gonadotropin, testosterone, and sperm parameters in nicotinamide/streptozotocin-induced diabetic mice (Ahangarpour et al., 2015). The root extract led to increased level of luteinizing hormone (LH), follicle stimulating hormone (FSH), and testosterone as well as enhancement in sperm viability only in diabetic mice compared with the control group, indicating A. lappa root extract to be a potentially effective treatment for male sterility arising from diabetic conditions.

Effect on NO Production

It was reported that arctigenin inhibited NO release by IFN-γ signal, whereas it significantly enhanced lipopolysaccharide-triggered NO production in RAW264.7 cells, suggesting that arctigenin may regulate immune responses in activated macrophages and lymphocytes including TNF-α and NO production and lymphocyte proliferation (Cho et al., 1999). Another study shows that arctigenin suppressed the overproduction of NO through down-regulation of iNOS expression and iNOS enzymatic activity in LPS-stimulated macrophage (Zhao et al., 2009). Besides, lappaol F and diarctigenin from Arctium lappa were shown to significantly inhibit NO production in the LPS-stimulated RAW264.7 cells with IC₅₀ values of 9.5 and 9.6 μM, respectively (Park et al., 2007).

Safety Considerations on Arctium Species

Several adverse effects have been reported in literature stemming from long-term use of A. lappa. For example, contact dermatitis might develop after several days of applying a burdock root plaster to a wound, or even as fast as within 12 h in some cases (Rodriguez et al., 1995). In one instance, anticholinergic poisoning has been reported upon oral consumption of A. lappa extract (Force, 2001). However, this poisoning later turned out to be caused by products that have been contaminated with root of belladonna (deadly nightshade). The latter herb contains the poisonous chemical atropine. Long-term consumption of burdock also has led to anaphylaxis in one case (Chan et al., 2011). Root oil made from A. lappa was also found to cause unfavorable physiological effects such as redness, and anaphylactic shock (Rodriguez et al., 1995; Lewis and Elvin-Lewis, 2003; Sasaki et al., 2003). Caution is advised for pregnant or nursing women to consume burdock or its extract, as it might have detrimental effects on the fetus (Chan et al., 2011). Burdock can also interfere with blood clotting. People who are already on blood thinning medications are advised not take it without approval from their doctors. Even though burdock is considered a ‘safe’ food, consuming it in large amounts should be avoided due to lack of large amount of safety studies on burdock. More in vivo studies are in particular needed on A. lappa to further evaluate its therapeutic potential and safe application window. Due to the presence of sesquiterpene lactones, the use of Arctium species should be avoided in patients with hypersensitivity to Asteraceae/Compositae (Chan et al., 2011).

Summary

In summary, the volatile and non-volatile secondary metabolites present in different parts of Arctium species showed pharmacological potential in the treatment of various diseases. The literature existing on extracts of different parts of A. lappa and isolated compounds demonstrates antipyretic, antimicrobial, diuretic, diaphoretic, hypoglycaemic, antioxidant, anti-inflammatory, anti-hepatotoxicity, antiulcer, antimutagenicity, and antitumour activities. Hence, Arctium species display a broad therapeutic potential but further studies are needed on potential risks associated with their application.

Author Contributions

All authors listed have made a substantial, direct and intellectual contribution to the work, and approved it for publication.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.