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. 2019 Jun 25;24(12):2344. doi: 10.3390/molecules24122344

Figure 1.

Figure 1

Effect of Mentha aquatica var. Kenting Water Mint essential oil (KWM-EO) on PDV cells. (A) PDV cells were treated with vehicle or the indicated concentrations of KWM-EO for 24 h. Cell viability (%) was determined by MTT assay. (B) PDV cells were incubated with KWM-EO in the presence or absence of 0.5 μM PLX4032 for 6 days, and colony formation was detected by staining cells with crystal violet. (C) PDV cells were seeded in Matrigel coated–transwell inserts and incubated with vehicle or KWM-EO in the presence or absence of 2 μM PLX4032 for 24 h. The invasive cells were stained with crystal violet. (D) PDV cell migratory ability was examined by wound healing assay. Cells were treated with vehicle or 50 μg/mL KWM-EO in the presence or absence of 2 μM PLX4032, and observed after 0, 6, 12, 24 h. Vehicle controls (C) were obtained from cells treated with 0.5% DMSO. The absorbance at 595 nm was obtained by dissolving crystal violet with 20% acetic acid. The data are representative of three independent experiments and are expressed as mean ± SD. Representative images are shown. P* < 0.05, P** < 0.01, P*** < 0.001 compared to vehicle control; P## < 0.01, P### < 0.001 compared to the PLX4032-treated group (ANOVA). AZD: AZD6244 (MEK inhibitor)