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. 2019 Jun 20;24(12):2291. doi: 10.3390/molecules24122291

Figure 5.

Figure 5

Effects of myrothecine A and mytoxin B on the expressions of caspases-3, -8, and -9, and cleaved caspases-3, -8, and -9 in SMMC-7721 cells. Cells were treated with different concentration of mytoxin B (0.01, 1, and 10 µg/mL) and myrothecine A (1, 10, and 50 µg/mL) for 24 h, respectively. Cell lysates under various treatments were isolated and analyzed by Western blot analysis (A). Relative amount of caspases-3, -8, and -9, and cleaved caspases-3, -8, and -9 in mytoxin B-treated cells (B–D). Relative amount of caspases-3, -8, and -9, and cleaved caspases-3, -8, and -9 in mytoxin A-treated cells (E–G). The experiment was independently repeated three times. Compared with negative control (NC), * P < 0.05, ** P < 0.01.