Table 2.
Comparison of genomic features among monkey and cattle picobirnaviruses (PBVs) and other PBV strains with a complete/near complete segment 2 sequence available.
| PBV | Genome Features | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Size (bp) | G+C Content (%) | ORF Features | 5′ UTR Features | 3′ UTR Features | ||||||||||
| ORF | Location (nt) | Length (nt) | Length (aa) | Frame | Length (nt) | G+C Content (%) | Beginning Bases | Length (nt) | G+C Content (%) | Ending Bases | ||||
| Reference genogroup I PBVs segment 2 sequences | ||||||||||||||
| Human 1 | 1745 | 46.36 | RdRp | 94-1698 | 1605 | 534 | +1 | 93 | 21.51 | GTAAA | 47 | 53.19 | CTGC | |
| Human 2 | 1696 | 43.63 | RdRp | 58-1650 | 1593 | 530 | +1 | 57 | 24.56 | GTAAA | 46 | 50.00 | CTGC | |
| Reference genogroup II PBV segment 2 sequence | ||||||||||||||
| Human 3 | 1674 | 43.91 | RdRp | 88-1641 | 1554 | 517 | +1 | 87 | 16.09 | GTAAA | 33 | 57.58 | CTC | |
| Genogroup I PBVs detected in this study | ||||||||||||||
| PBVs (Clade) | GenBank accession no. | |||||||||||||
| Monkey/11T-1 (M-R1;) | KY120190 | 1678 | 42.55 | RdRp | 28-1632 | 1605 | 534 | +1 | 27 | 14.81 | GTAAA | 46 | 52.17 | CTGC |
| Monkey/11T-2 (M-R2) | KY120191 | 1681 | 41.05 | RdRp | 47-1636 | 1590 | 529 | +2 | 46 | 26.09 | GTAAA | 45 | 51.11 | CTGC |
| Monkey/35T (M-R1) | KY120193 | 1678 | 42.61 | RdRp | 28-1632 | 1605 | 534 | +1 | 27 | 14.81 | GTAAA | 46 | 52.17 | CTGC |
| Monkey/21T (M-R1) | KY120192 | 1678 | 42.49 | RdRp | 28-1632 | 1605 | 534 | +1 | 27 | 14.81 | GTAAA | 46 | 52.17 | CTGC |
| Monkey/1R-1 4 (M-R3) | KY120194 | 1613 | 42.72 | RdRp | 2-1564 | 1563 | 520 | NA | NA | NA | NA | 49 | 53.06 | CTGC |
| Monkey/1R-2 (M-R2) | KY120182 | 1681 | 41.52 | RdRp | 47-1636 | 1590 | 529 | +2 | 46 | 26.09 | GTAAA | 45 | 51.11 | CTGC |
| Monkey/2R (M-R1) | KY120183 | 1678 | 42.37 | RdRp | 28-1632 | 1605 | 534 | +1 | 27 | 14.81 | GTAAA | 46 | 50.00 | CTGC |
| Monkey/12R (M-R1) | KY120184 | 1678 | 42.61 | RdRp | 28-1632 | 1605 | 534 | +1 | 27 | 14.81 | GTAAA | 46 | 52.17 | CTGC |
| Monkey/13R-1 (M-R3) | KY120185 | 1734 | 41.64 | RdRp | 75-1685 | 1611 | 536 | +3 | 74 | 25.68 | GTAAA | 49 | 53.06 | CTGC |
| Monkey/13R-2 (M-R1) | KY120186 | 1678 | 42.55 | RdRp | 28-1632 | 1605 | 534 | +1 | 27 | 14.81 | GTAAA | 46 | 52.17 | CTGC |
| Monkey/14R (M-R1) | KY120187 | 1678 | 42.55 | RdRp | 28-1632 | 1605 | 534 | +1 | 27 | 14.81 | GTAAA | 46 | 52.17 | CTGC |
| Monkey/15R-1 (M-R1) | KY120188 | 1678 | 42.31 | RdRp | 28-1632 | 1605 | 534 | +1 | 27 | 14.81 | GTAAA | 46 | 50.00 | CTGC |
| Monkey/15R-2 (M-R3) | KY120189 | 1734 | 41.64 | RdRp | 75-1685 | 1611 | 536 | +3 | 74 | 25.68 | GTAAA | 49 | 53.06 | CTGC |
| Cattle/C345N-2 | KY120170 | 1671 | 43.93 | RdRp | 49-1629 | 1581 | 526 | +1 | 48 | 25.00 | GTAAA | 42 | 52.38 | CATC |
| Cattle/C345N-3 | KY120171 | 1694 | 42.15 | RdRp | 44-1657 | 1614 | 537 | +2 | 43 | 23.26 | GTAAA | 37 | 59.46 | CTGC |
| Cattle/C346N-1 | KY120172 | 1701 | 47.68 | RdRp | 49-1656 | 1608 | 535 | +1 | 48 | 29.17 | GTAAA | 45 | 44.44 | CTGC |
| Cattle/C346N-2 | KY120173 | 1696 | 44.99 | RdRp | 52-1647 | 1596 | 531 | +1 | 51 | 27.45 | GTAAA | 49 | 46.94 | CTGC |
| Cattle/C361N | KY120174 | 1696 | 44.58 | RdRp | 49-1653 | 1605 | 534 | +1 | 48 | 35.42 | GTAAA | 43 | 44.19 | CTGC |
| Cattle/C343R-1 | KY120175 | 1692 | 44.03 | RdRp | 50-1642 | 1593 | 530 | +2 | 49 | 20.41 | GTAAA | 50 | 58.00 | CTGC |
| Cattle/C343R-2 | KY120176 | 1695 | 45.25 | RdRp | 48-1652 | 1605 | 534 | +3 | 47 | 25.53 | GTAAA | 43 | 44.19 | CTGC |
| Cattle/C369R-1 | KY120177 | 1736 | 42.45 | RdRp | 36-1721 | 1686 | 561 | +3 | 35 | 22.86 | GTAAA | 15 | 40.00 | CTTC |
| Cattle/C369R-2 5 | KY120179 | 1659 | 44.91 | RdRp | 24-1616 | 1593 | 530 | NA | 23 | 30.43 | NA | 43 | 55.81 | CTGC |
| Cattle/C374R-1 5 | KY120180 | 1651 | 44.16 | RdRp | 8-1606 | 1599 | 532 | NA | 7 | 28.57 | NA | 45 | 55.56 | CTGC |
| Cattle/C374R-2 5 | KY120181 | 1669 | 47.15 | RdRp | 24-1625 | 1602 | 533 | NA | 23 | 34.78 | NA | 44 | 47.73 | CTGC |
| Genogroup II PBVs detected in this study | ||||||||||||||
| Cattle/C372N | KY120178 | 1622 | 40.44 | RdRp | 55-1599 | 1545 | 514 | +1 | 54 | 16.67% | GTAAA | 23 | 52.17 | CTCC |
1 The accession number of human PBV segment 2 is NC_007027; 2 the accession number of human PBV segment 2 is AF246939; 3 the accession number of human PBV segment 2 is AF246940; 4 the partial segment 2 with a near complete RdRp sequence; 5 the partial segment 2 with complete RdRp sequences; NA, not available; T, throat swab; R, rectal swab; N, nasal swab.