Table 2.

Time to the most recent common ancestor (TMRCA) estimates of HPAI H5N5 genotypes. Estimated median TMRCA for each gene segment of highly pathogenic avian influenza (HPAI) H5N5 viruses of genotype H5N5-GT2, the European H5N5-GT2 viruses and H5N5-GT3 viruses with 95% highest posterior density (HPD) intervals. The nodes for which the median TMRCAs estimates were determined are depicted in the time-scaled phylogenetic trees in Figure S3. PB2, polymerase basic protein 2; PB1, polymerase basic protein 1; PA, polymerase acidic protein; HA, hemagglutinin; NP, nucleoprotein; NA, neuraminidase; MP, matrix protein; NS, nonstructural protein.

	H5N5-GT2 Viruses (Node 1)				European H5N5-GT2 Viruses (Node 2)				H5N5-GT3 Viruses (Node 3)
Gene Segment	Median TMRCA	Lower 95% HPD	Upper 95% HPD	Posterior	Median TMRCA	Lower 95% HPD	Upper 95% HPD	Posterior	Median TMRCA	Lower 95% HPD	Upper 95% HPD	Posterior
PB2	Mar-2016	Aug-2015	Aug-2016	0.9997	Sep-2016	May-2016	Nov-2016	0.9997	Oct-2016	Aug-2016	Nov-2016	0.4823
PB1	Dec-2015	Apr-2015	Jun-2016	0.9983	Jul-2016	Feb-2016	Oct-2016	0.9982	Oct-2016	May-2016	Jan-2017	0.9971
PA	Aug-2016	Jun-2016	Sep-2016	0.9982	Oct-2016	Sep-2016	Nov-2016	0.9995	Nov-2016	Oct-2016	Dec-2016	0.0942
HA	Jun-2016	Jan-2016	Aug-2016	0.9995	Oct-2016	Oct-2016	Jun-2016	0.9991	Dec-2016	Oct-2016	Jan-2017	0.9991
NP	Mar-2016	Aug-2015	Aug-2016	0.9988	Aug-2016	Apr-2016	Aug-2016	0.9988	Sep-2016	Mar-2016	Dec-2016	0.9991
NA	Nov-2015	Apr-2015	May-2016	0.9997	Apr-2016	Nov-2015	Aug-2016	0.9929	Oct-2016	Jun-2016	Jan-2017	0.9713
MP	Aug-2016	Jun-2016	Sep-2016	0.9929	Nov-2016	Nov-2016	Dec-2016	0.9928	Jan-2017	Dec-2016	Jan-2017	0.0325
NS	Jul-2016	Mar-2016	Sep-2016	0.9992	Nov-2016	Jun-2016	Aug-2016	0.9835	Dec-2016	Nov-2016	Dec-2016	0.0038