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. 2019 Jul 1;8:e46315. doi: 10.7554/eLife.46315

Figure 2. Elevated Hipk induces robust expression of a subset of glycolytic genes.

Figure 2.

(a, b) qRT-PCR analysis of glycolytic gene expression in hipk-expressing discs (dpp>hipk) relative to control discs (dpp>GFP) (a). Data are mean ± sem. n = 3 independent experiments with samples pooled from 10 to 20 wing discs per genotype. Schematic diagram of glycolysis (b). Fold changes are shown in brackets. Glycolytic enzymes with more than 1.5-fold transcriptional upregulation in hipk-expressing discs are marked in red. Bi- and uni-directional arrows indicate the reversible and essentially irreversible steps in glycolysis, respectively. Dashed arrow (blue) shows the positive allosteric regulation of Pfk by fructose-2,6-BP. Full names of the glycolytic genes are provided in Supplementary file 1. Exact p values are shown and calculated using unpaired two-tailed t-test. (c–d) Expression of Ldh-GFP (green), an enhancer trap to monitor Ldh expression, in control (genotype: Ldh-GFP/+) (c) and hipk-expressing wing discs (genotype: Ldh-GFP/dpp>hipk) (d). DAPI staining for DNA (blue) reveals tissue morphology. White arrowhead in d’’ indicates marked induction of Ldh expression. Scale bars, 50 μm.

Figure 2—source data 1. Expression profile of glycolytic genes inhipk-expressing discs.
elife-46315-fig2-data1.xlsx (11.3KB, xlsx)
DOI: 10.7554/eLife.46315.008