(a) Intravascular PO2 in the diving arterioles (red symbols) and venules (blue symbols) across cortical layers I-V (11 arterioles, 14 venules, from n = 7 mice). (b) SO2 in the diving arterioles (red symbols) and venules (blue symbols) across cortical layers I-V, and the depth-dependent OEF (DOEF, black symbols). For each diving arteriole or surfacing venule in a and b, PO2 was tracked from the cortical surface down to the cortical depth of 600 µm. Data are expressed as mean ± SEM. Please note that the error bars may be too small to be visible.