Fig. 5.

Ant-inflammatory efficacy of secreted ANXA1 in AHF mouse model. (a) H&E staining of liver sections from control and AHF (CCl₄-induced) mice, as well as AHF mice receiving CM from AF-MSC-shscramble and AF-MSC-shANXA1 cultures. (Original magnification x20, scale bar: 100 μm). Arrows show necrotic areas. (b) Serum AST and ALT levels were measured in heathy mice (control), AHF mice, AHF mice that were administered CM from AF-MSC-shscramble or CM from AF-MSC-ANXA1 cultures. Data presented as the mean ± SD of five mice per group and analyzed by Student's t-test. P-values were estimated compared to AHF mice (*, p < 0.05; **, p < 0.01, Student's t-test). (c) Analysis of mouse serum cytokine levels, after treatment with shANXA1- or shscramble-CM. Mouse serum levels for IFN-γ, TNF and IL-10 from healthy mice, AHF mice or AHF mice that received shANXA1- and shscramble-CM, 24 h after administration. Each assay was performed in duplicates. Data are presented as the mean ± SD for at least 3 independent experiments and were analyzed by Student's t-test method (*, p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).