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. 2019 Jul 9;9(16):4795–4810. doi: 10.7150/thno.30988

Figure 7.

Figure 7

CF129 is downregulated by HIF-1α during Hypoxia. (A) A putative HREs (ACGTGC) were found in the promoter of CF129 gene. (B) PANC-1 cells were cultured during hypoxia, and CF129 mRNA and HIF-1α protein were measured via qRT-PCR and western blotting, respectively, at indicated timepoints. (C) CF129 expression of siHIF-1α-transfected PANC-1 cells was compared to hypoxic siNC-transfected PANC-1 cells via qRT-PCR. (D) Single molecule RNA FISH detection of CF129 (green) in hypoxic or normoxic PANC-1 cells with siNC or siHIF-1α transfection. Nucleus was counterstained with DAPI. (E) ChIP was used to assess hypoxic or normoxic PANC-1 cells transfected with siNC or siHIF-1α by using anti-HIF-1α antibody. (F) PANC-1 cells underwent transfection using WT or MUT plasmid as reporter cells, which were further transfected using siNC or siHIF-1α under normoxia or hypoxia. The luciferase activity was measured and normalized. (G) Western blotting detection of HIF-1α, p53, and FOXC2 in hypoxic or normoxic PANC-1 cells transfected with siNC or siHIF-1α. (H) The protein level of PANC-1 cells transfected with control or CF129-OE under normoxia or hypoxia was analyzed by Western blot.